YER166W / DNF1

Aminophospholipid translocase (flippase) that localizes primarily to the plasma membrane; contributes to endocytosis, protein transport and cell polarity; type 4 P-type ATPase

Zygosity: Homozygous strain
fixedexpanded
Profile for YER166W / DNF1

Click on Significant Values for Screen Details ID:SGTC_120|Compound:rhodamine 6G|FD-Score:3.66|P-value:2.59E-4 ID:SGTC_173|Compound:1273-0061|FD-Score:-3.32|P-value:7.69E-4 ID:SGTC_363|Compound:0455-0021|FD-Score:4.35|P-value:1.84E-5 ID:SGTC_416|Compound:estradiol valerate|FD-Score:3.87|P-value:1.20E-4 ID:SGTC_424|Compound:splitomicin|FD-Score:-6.84|P-value:3.45E-11 ID:SGTC_425|Compound:3825-7017|FD-Score:3.54|P-value:4.02E-4 ID:SGTC_435|Compound:c-PAF|FD-Score:-3.46|P-value:4.75E-4 ID:SGTC_641|Compound:4092-0595|FD-Score:3.77|P-value:1.77E-4 ID:SGTC_696|Compound:1560-0013|FD-Score:4.77|P-value:3.03E-6 ID:SGTC_756|Compound:0508-1999|FD-Score:3.61|P-value:3.09E-4 ID:SGTC_933|Compound:2914-0560|FD-Score:3.45|P-value:5.39E-4 ID:SGTC_1092|Compound:toremifene|FD-Score:3.72|P-value:2.13E-4 ID:SGTC_1132|Compound:3456-3614|FD-Score:3.51|P-value:4.36E-4 ID:SGTC_1174|Compound:0739-0097|FD-Score:4.26|P-value:2.68E-5 ID:SGTC_1193|Compound:1488-0126|FD-Score:3.29|P-value:9.13E-4 ID:SGTC_1194|Compound:1488-0194|FD-Score:5.65|P-value:4.12E-8 ID:SGTC_1217|Compound:1183-1422|FD-Score:-3.52|P-value:3.84E-4 ID:SGTC_1235|Compound:benzylparaben|FD-Score:4.29|P-value:2.37E-5 ID:SGTC_1242|Compound:0402-0103|FD-Score:3.53|P-value:4.15E-4 ID:SGTC_1504|Compound:k297-0033|FD-Score:4.28|P-value:2.49E-5 ID:SGTC_1557|Compound:sumaresinolic acid|FD-Score:3.29|P-value:9.12E-4 ID:SGTC_1624|Compound:st003710|FD-Score:3.48|P-value:4.79E-4 ID:SGTC_1754|Compound:st042065|FD-Score:3.66|P-value:2.63E-4 ID:SGTC_1800|Compound:st050079|FD-Score:3.63|P-value:2.87E-4 ID:SGTC_1841|Compound:st054148|FD-Score:3.29|P-value:9.11E-4 ID:SGTC_1908|Compound:st060353|FD-Score:4.54|P-value:8.15E-6 ID:SGTC_1959|Compound:st077091|FD-Score:4.03|P-value:6.57E-5 ID:SGTC_2033|Compound:5192550|FD-Score:3.69|P-value:2.37E-4 ID:SGTC_2133|Compound:5320837|FD-Score:5.11|P-value:6.19E-7 ID:SGTC_2265|Compound:7929201|FD-Score:4.40|P-value:1.50E-5 ID:SGTC_2415|Compound:st077333|FD-Score:-3.82|P-value:1.33E-4 ID:SGTC_2434|Compound:5670830|FD-Score:-4.65|P-value:4.68E-6 ID:SGTC_2466|Compound:5983409|FD-Score:3.50|P-value:4.59E-4 ID:SGTC_2513|Compound:baicalein|FD-Score:-3.44|P-value:5.07E-4 ID:SGTC_2520|Compound:obtusaquinone|FD-Score:4.74|P-value:3.42E-6 ID:SGTC_2561|Compound:acetosyringone|FD-Score:-3.35|P-value:6.92E-4 ID:SGTC_2653|Compound:dihydrofissinolide|FD-Score:3.31|P-value:8.60E-4 ID:SGTC_2781|Compound:5567276|FD-Score:5.13|P-value:5.70E-7 ID:SGTC_2844|Compound:9010103|FD-Score:4.40|P-value:1.50E-5 ID:SGTC_2855|Compound:9016525|FD-Score:3.92|P-value:1.00E-4 ID:SGTC_2996|Compound:9066192|FD-Score:-3.49|P-value:4.38E-4 ID:SGTC_3049|Compound:9092223|FD-Score:3.31|P-value:8.62E-4 ID:SGTC_3084|Compound:9116877|FD-Score:3.36|P-value:7.15E-4 ID:SGTC_3148|Compound:9097893|FD-Score:3.49|P-value:4.65E-4 ID:SGTC_3225|Compound:9130819|FD-Score:-4.04|P-value:5.88E-5 ID:SGTC_3245|Compound:9134821|FD-Score:4.91|P-value:1.59E-6 ID:SGTC_3264|Compound:9137442|FD-Score:3.46|P-value:5.29E-4 ID:SGTC_120|Compound:rhodamine 6G|FD-Score:3.66|P-value:2.59E-4 ID:SGTC_173|Compound:1273-0061|FD-Score:-3.32|P-value:7.69E-4 ID:SGTC_363|Compound:0455-0021|FD-Score:4.35|P-value:1.84E-5 ID:SGTC_416|Compound:estradiol valerate|FD-Score:3.87|P-value:1.20E-4 ID:SGTC_424|Compound:splitomicin|FD-Score:-6.84|P-value:3.45E-11 ID:SGTC_425|Compound:3825-7017|FD-Score:3.54|P-value:4.02E-4 ID:SGTC_435|Compound:c-PAF|FD-Score:-3.46|P-value:4.75E-4 ID:SGTC_641|Compound:4092-0595|FD-Score:3.77|P-value:1.77E-4 ID:SGTC_696|Compound:1560-0013|FD-Score:4.77|P-value:3.03E-6 ID:SGTC_756|Compound:0508-1999|FD-Score:3.61|P-value:3.09E-4 ID:SGTC_933|Compound:2914-0560|FD-Score:3.45|P-value:5.39E-4 ID:SGTC_1092|Compound:toremifene|FD-Score:3.72|P-value:2.13E-4 ID:SGTC_1132|Compound:3456-3614|FD-Score:3.51|P-value:4.36E-4 ID:SGTC_1174|Compound:0739-0097|FD-Score:4.26|P-value:2.68E-5 ID:SGTC_1193|Compound:1488-0126|FD-Score:3.29|P-value:9.13E-4 ID:SGTC_1194|Compound:1488-0194|FD-Score:5.65|P-value:4.12E-8 ID:SGTC_1217|Compound:1183-1422|FD-Score:-3.52|P-value:3.84E-4 ID:SGTC_1235|Compound:benzylparaben|FD-Score:4.29|P-value:2.37E-5 ID:SGTC_1242|Compound:0402-0103|FD-Score:3.53|P-value:4.15E-4 ID:SGTC_1504|Compound:k297-0033|FD-Score:4.28|P-value:2.49E-5 ID:SGTC_1557|Compound:sumaresinolic acid|FD-Score:3.29|P-value:9.12E-4 ID:SGTC_1624|Compound:st003710|FD-Score:3.48|P-value:4.79E-4 ID:SGTC_1754|Compound:st042065|FD-Score:3.66|P-value:2.63E-4 ID:SGTC_1800|Compound:st050079|FD-Score:3.63|P-value:2.87E-4 ID:SGTC_1841|Compound:st054148|FD-Score:3.29|P-value:9.11E-4 ID:SGTC_1908|Compound:st060353|FD-Score:4.54|P-value:8.15E-6 ID:SGTC_1959|Compound:st077091|FD-Score:4.03|P-value:6.57E-5 ID:SGTC_2033|Compound:5192550|FD-Score:3.69|P-value:2.37E-4 ID:SGTC_2133|Compound:5320837|FD-Score:5.11|P-value:6.19E-7 ID:SGTC_2265|Compound:7929201|FD-Score:4.40|P-value:1.50E-5 ID:SGTC_2415|Compound:st077333|FD-Score:-3.82|P-value:1.33E-4 ID:SGTC_2434|Compound:5670830|FD-Score:-4.65|P-value:4.68E-6 ID:SGTC_2466|Compound:5983409|FD-Score:3.50|P-value:4.59E-4 ID:SGTC_2513|Compound:baicalein|FD-Score:-3.44|P-value:5.07E-4 ID:SGTC_2520|Compound:obtusaquinone|FD-Score:4.74|P-value:3.42E-6 ID:SGTC_2561|Compound:acetosyringone|FD-Score:-3.35|P-value:6.92E-4 ID:SGTC_2653|Compound:dihydrofissinolide|FD-Score:3.31|P-value:8.60E-4 ID:SGTC_2781|Compound:5567276|FD-Score:5.13|P-value:5.70E-7 ID:SGTC_2844|Compound:9010103|FD-Score:4.40|P-value:1.50E-5 ID:SGTC_2855|Compound:9016525|FD-Score:3.92|P-value:1.00E-4 ID:SGTC_2996|Compound:9066192|FD-Score:-3.49|P-value:4.38E-4 ID:SGTC_3049|Compound:9092223|FD-Score:3.31|P-value:8.62E-4 ID:SGTC_3084|Compound:9116877|FD-Score:3.36|P-value:7.15E-4 ID:SGTC_3148|Compound:9097893|FD-Score:3.49|P-value:4.65E-4 ID:SGTC_3225|Compound:9130819|FD-Score:-4.04|P-value:5.88E-5 ID:SGTC_3245|Compound:9134821|FD-Score:4.91|P-value:1.59E-6 ID:SGTC_3264|Compound:9137442|FD-Score:3.46|P-value:5.29E-4

Top fitness defect scores for YER166W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1194 1488-0194 5.65 225.0 µM 4.12E-8 8 8.07E-9
2 SGTC_2781 5567276 5.13 71.4 µM 5.70E-7 22 1.47E-7
3 SGTC_2133 5320837 5.11 200.0 µM 6.19E-7 ERG2 10 1.61E-7
4 SGTC_3245 9134821 4.91 49.5 µM 1.59E-6 4 4.56E-7
5 SGTC_696 1560-0013 4.77 611.0 µM 3.03E-6 13 9.27E-7
6 SGTC_2520 obtusaquinone 4.74 4.7 µM 3.42E-6 7 1.06E-6
7 SGTC_1908 st060353 4.54 56.5 µM 8.15E-6 RPP1 & pyrimidine depletion 25 2.77E-6
8 SGTC_2844 9010103 4.40 26.0 µM 1.50E-5 21 5.40E-6
9 SGTC_2265 7929201 4.40 200.0 µM 1.50E-5 17 5.41E-6
10 SGTC_363 0455-0021 4.35 3.1 µM 1.84E-5 azole & statin 52 6.80E-6
11 SGTC_1235 benzylparaben 4.29 21.5 µM 2.37E-5 8 8.95E-6
12 SGTC_1504 k297-0033 4.28 30.0 µM 2.49E-5 8 9.49E-6
13 SGTC_1174 0739-0097 4.26 51.1 µM 2.68E-5 redox potentiating 21 1.03E-5
14 SGTC_1959 st077091 4.03 55.8 µM 6.57E-5 RPP1 & pyrimidine depletion 46 2.76E-5
15 SGTC_2855 9016525 3.92 58.4 µM 1.00E-4 16 4.39E-5
16 SGTC_416 estradiol valerate 3.87 100.0 µM 1.20E-4 10 5.38E-5
17 SGTC_641 4092-0595 3.77 30.0 µM 1.77E-4 14 8.23E-5
18 SGTC_1092 toremifene 3.72 6.3 µM 2.13E-4 25 1.01E-4
19 SGTC_2033 5192550 3.69 145.0 µM 2.37E-4 20 1.14E-4
20 SGTC_120 rhodamine 6G 3.66 52.3 µM 2.59E-4 mitochondrial processes 45 1.25E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.140 4.60E-16 YLR215C CDC123 het Protein involved in nutritional control of the cell cycle; regulates abundance of the translation initiation factor eIF2; ortholog of human D123 protein
0.094 4.82E-8 YNL086W_p SNN1_p hom Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; green fluorescent protein (GFP)-fusion protein localizes to endosomes
0.084 1.26E-6 YOL089C HAL9 hom Putative transcription factor containing a zinc finger; overexpression increases salt tolerance through increased expression of the ENA1 (Na+/Li+ extrusion pump) gene while gene disruption decreases both salt tolerance and ENA1 expression; HAL9 has a paralog, TBS1, that arose from the whole genome duplication
0.080 3.36E-6 YJR036C HUL4 hom Protein with similarity to hect domain E3 ubiquitin-protein ligases; not essential for viability; found in association with Trf4 in TRAMP complex
0.080 3.42E-6 YLR404W FLD1 hom Seipin protein involved in lipid droplet morphology, number, and size; proposed to be involved in lipid metabolism; related to the human BSCL2 which is associated with lipodystrophy
0.078 6.65E-6 YNR032W PPG1 hom Putative serine/threonine protein phosphatase of the type 2A-like phosphatase family, required for glycogen accumulation; interacts with Tap42p, which binds to and regulates other protein phosphatases
0.078 6.69E-6 YEL011W GLC3 hom Glycogen branching enzyme, involved in glycogen accumulation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
0.077 7.30E-6 YEL063C CAN1 hom Plasma membrane arginine permease; requires phosphatidyl ethanolamine (PE) for localization, exclusively associated with lipid rafts; mutation confers canavanine resistance; CAN1 has a paralog, ALP1, that arose from the whole genome duplication
0.074 1.57E-5 YOR275C RIM20 hom Protein involved in proteolytic activation of Rim101p in response to alkaline pH; PalA/AIP1/Alix family member; interaction with the ESCRT-III subunit Snf7p suggests a relationship between pH response and multivesicular body formation
0.073 2.05E-5 YFR023W PES4 hom Poly(A) binding protein, suppressor of DNA polymerase epsilon mutation; PES4 has a paralog, MIP6, that arose from the whole genome duplication
0.072 3.25E-5 YNL038W GPI15 het Protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; homologous to the human PIG-H protein
0.068 7.20E-5 YPL152W-A_p YPL152W-A_p hom Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
0.065 1.52E-4 YKR073C_d YKR073C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.064 2.06E-4 YPR069C SPE3 hom Spermidine synthase, involved in biosynthesis of spermidine and also in biosynthesis of pantothenic acid; spermidine is required for growth of wild-type cells
0.064 2.30E-4 YHR131C_p YHR131C_p hom Putative protein of unknown function; GFP-fusion protein localizes to the cytoplasm; overexpression causes cell cycle delay or arrest; contains a PH domain and binds phosphatidylinositols and other lipids in a large-scale study