YER168C / CCA1

ATP (CTP):tRNA-specific tRNA nucleotidyltransferase; different forms targeted to the nucleus, cytosol, and mitochondrion are generated via the use of multiple transcriptional and translational start sites

Zygosity: Heterozygous strain
fixedexpanded
Profile for YER168C / CCA1

Click on Significant Values for Screen Details ID:SGTC_86|Compound:0417-1630|FD-Score:3.08|P-value:7.96E-4 ID:SGTC_249|Compound:wiskostatin|FD-Score:3.07|P-value:8.24E-4 ID:SGTC_753|Compound:1322-0200|FD-Score:3.51|P-value:1.62E-4 ID:SGTC_813|Compound:k007-0158|FD-Score:3.26|P-value:4.18E-4 ID:SGTC_932|Compound:2857-0803|FD-Score:3.76|P-value:5.89E-5 ID:SGTC_1036|Compound:k216-0215|FD-Score:-4.47|P-value:2.77E-6 ID:SGTC_1053|Compound:3366-3204|FD-Score:4.79|P-value:4.78E-7 ID:SGTC_1076|Compound:itraconazole|FD-Score:3.55|P-value:1.39E-4 ID:SGTC_1118|Compound:chelidonine|FD-Score:3.82|P-value:4.57E-5 ID:SGTC_1511|Compound:0906-3109|FD-Score:4.47|P-value:2.46E-6 ID:SGTC_1512|Compound:0259-0544|FD-Score:-3.75|P-value:6.85E-5 ID:SGTC_1517|Compound:deoxycholic acid methyl ester|FD-Score:3.10|P-value:7.50E-4 ID:SGTC_1528|Compound:arabinosylhypoxanthine|FD-Score:3.58|P-value:1.23E-4 ID:SGTC_1593|Compound:justicidin b . 9-(1,3-benzodioxol-5-yl)- 6,7-dimethoxynaphtho[2,3-c]furan-1(3h)-one|FD-Score:3.48|P-value:1.82E-4 ID:SGTC_1602|Compound:st001878|FD-Score:-3.04|P-value:9.98E-4 ID:SGTC_1731|Compound:st035964|FD-Score:3.85|P-value:4.12E-5 ID:SGTC_1787|Compound:fluconazole|FD-Score:3.36|P-value:2.95E-4 ID:SGTC_2015|Compound:5119600|FD-Score:4.41|P-value:3.23E-6 ID:SGTC_2019|Compound:4016004|FD-Score:3.54|P-value:1.45E-4 ID:SGTC_2040|Compound:5100729|FD-Score:3.12|P-value:7.06E-4 ID:SGTC_2049|Compound:5262831|FD-Score:3.89|P-value:3.46E-5 ID:SGTC_2054|Compound:5233173|FD-Score:-4.40|P-value:3.84E-6 ID:SGTC_2079|Compound:5212404|FD-Score:3.12|P-value:6.95E-4 ID:SGTC_2122|Compound:5258151|FD-Score:3.27|P-value:4.01E-4 ID:SGTC_2128|Compound:5255449|FD-Score:-3.21|P-value:5.52E-4 ID:SGTC_2146|Compound:5545642|FD-Score:-3.07|P-value:9.27E-4 ID:SGTC_2188|Compound:6126565|FD-Score:3.34|P-value:3.16E-4 ID:SGTC_2200|Compound:6600604|FD-Score:3.14|P-value:6.56E-4 ID:SGTC_2477|Compound:5967686|FD-Score:3.61|P-value:1.10E-4 ID:SGTC_2765|Compound:myclobutanil|FD-Score:3.02|P-value:0.00100 ID:SGTC_2780|Compound:5215298|FD-Score:-3.32|P-value:3.73E-4 ID:SGTC_2789|Compound:5281112|FD-Score:3.99|P-value:2.22E-5 ID:SGTC_2796|Compound:7949634|FD-Score:-3.15|P-value:6.85E-4 ID:SGTC_2801|Compound:7907800|FD-Score:-3.21|P-value:5.59E-4 ID:SGTC_2803|Compound:7707263|FD-Score:4.03|P-value:1.87E-5 ID:SGTC_2943|Compound:9068529|FD-Score:4.66|P-value:9.37E-7 ID:SGTC_2994|Compound:9053520|FD-Score:6.34|P-value:4.55E-11 ID:SGTC_2998|Compound:9066762|FD-Score:5.17|P-value:6.31E-8 ID:SGTC_3031|Compound:9088762|FD-Score:3.13|P-value:6.71E-4 ID:SGTC_3052|Compound:9092717|FD-Score:-3.43|P-value:2.44E-4 ID:SGTC_3102|Compound:9121669|FD-Score:3.29|P-value:3.79E-4 ID:SGTC_3170|Compound:9103054|FD-Score:-3.29|P-value:4.20E-4 ID:SGTC_3337|Compound:9144342|FD-Score:-3.66|P-value:9.87E-5 ID:SGTC_3341|Compound:9147222|FD-Score:5.12|P-value:8.17E-8 ID:SGTC_86|Compound:0417-1630|FD-Score:3.08|P-value:7.96E-4 ID:SGTC_249|Compound:wiskostatin|FD-Score:3.07|P-value:8.24E-4 ID:SGTC_753|Compound:1322-0200|FD-Score:3.51|P-value:1.62E-4 ID:SGTC_813|Compound:k007-0158|FD-Score:3.26|P-value:4.18E-4 ID:SGTC_932|Compound:2857-0803|FD-Score:3.76|P-value:5.89E-5 ID:SGTC_1036|Compound:k216-0215|FD-Score:-4.47|P-value:2.77E-6 ID:SGTC_1053|Compound:3366-3204|FD-Score:4.79|P-value:4.78E-7 ID:SGTC_1076|Compound:itraconazole|FD-Score:3.55|P-value:1.39E-4 ID:SGTC_1118|Compound:chelidonine|FD-Score:3.82|P-value:4.57E-5 ID:SGTC_1511|Compound:0906-3109|FD-Score:4.47|P-value:2.46E-6 ID:SGTC_1512|Compound:0259-0544|FD-Score:-3.75|P-value:6.85E-5 ID:SGTC_1517|Compound:deoxycholic acid methyl ester|FD-Score:3.10|P-value:7.50E-4 ID:SGTC_1528|Compound:arabinosylhypoxanthine|FD-Score:3.58|P-value:1.23E-4 ID:SGTC_1593|Compound:justicidin b . 9-(1,3-benzodioxol-5-yl)- 6,7-dimethoxynaphtho[2,3-c]furan-1(3h)-one|FD-Score:3.48|P-value:1.82E-4 ID:SGTC_1602|Compound:st001878|FD-Score:-3.04|P-value:9.98E-4 ID:SGTC_1731|Compound:st035964|FD-Score:3.85|P-value:4.12E-5 ID:SGTC_1787|Compound:fluconazole|FD-Score:3.36|P-value:2.95E-4 ID:SGTC_2015|Compound:5119600|FD-Score:4.41|P-value:3.23E-6 ID:SGTC_2019|Compound:4016004|FD-Score:3.54|P-value:1.45E-4 ID:SGTC_2040|Compound:5100729|FD-Score:3.12|P-value:7.06E-4 ID:SGTC_2049|Compound:5262831|FD-Score:3.89|P-value:3.46E-5 ID:SGTC_2054|Compound:5233173|FD-Score:-4.40|P-value:3.84E-6 ID:SGTC_2079|Compound:5212404|FD-Score:3.12|P-value:6.95E-4 ID:SGTC_2122|Compound:5258151|FD-Score:3.27|P-value:4.01E-4 ID:SGTC_2128|Compound:5255449|FD-Score:-3.21|P-value:5.52E-4 ID:SGTC_2146|Compound:5545642|FD-Score:-3.07|P-value:9.27E-4 ID:SGTC_2188|Compound:6126565|FD-Score:3.34|P-value:3.16E-4 ID:SGTC_2200|Compound:6600604|FD-Score:3.14|P-value:6.56E-4 ID:SGTC_2477|Compound:5967686|FD-Score:3.61|P-value:1.10E-4 ID:SGTC_2765|Compound:myclobutanil|FD-Score:3.02|P-value:0.00100 ID:SGTC_2780|Compound:5215298|FD-Score:-3.32|P-value:3.73E-4 ID:SGTC_2789|Compound:5281112|FD-Score:3.99|P-value:2.22E-5 ID:SGTC_2796|Compound:7949634|FD-Score:-3.15|P-value:6.85E-4 ID:SGTC_2801|Compound:7907800|FD-Score:-3.21|P-value:5.59E-4 ID:SGTC_2803|Compound:7707263|FD-Score:4.03|P-value:1.87E-5 ID:SGTC_2943|Compound:9068529|FD-Score:4.66|P-value:9.37E-7 ID:SGTC_2994|Compound:9053520|FD-Score:6.34|P-value:4.55E-11 ID:SGTC_2998|Compound:9066762|FD-Score:5.17|P-value:6.31E-8 ID:SGTC_3031|Compound:9088762|FD-Score:3.13|P-value:6.71E-4 ID:SGTC_3052|Compound:9092717|FD-Score:-3.43|P-value:2.44E-4 ID:SGTC_3102|Compound:9121669|FD-Score:3.29|P-value:3.79E-4 ID:SGTC_3170|Compound:9103054|FD-Score:-3.29|P-value:4.20E-4 ID:SGTC_3337|Compound:9144342|FD-Score:-3.66|P-value:9.87E-5 ID:SGTC_3341|Compound:9147222|FD-Score:5.12|P-value:8.17E-8

Top fitness defect scores for YER168C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2994 9053520 6.34 71.4 µM 4.55E-11 RPP1 & pyrimidine depletion 2 1.14E-10 1.35
2 SGTC_2998 9066762 5.17 71.4 µM 6.31E-8 RPP1 & pyrimidine depletion 4 1.18E-7 0.25
3 SGTC_3341 9147222 5.12 25.9 µM 8.17E-8 RNA processing & uracil transport 4 1.52E-7 0.56
4 SGTC_1053 3366-3204 4.79 134.0 µM 4.78E-7 cell wall 4 8.28E-7 0.46
5 SGTC_2943 9068529 4.66 45.3 µM 9.37E-7 10 1.58E-6 0.06
6 SGTC_1511 0906-3109 4.47 28.5 µM 2.46E-6 excess fatty acid 7 4.00E-6 0.04
7 SGTC_2015 5119600 4.41 184.0 µM 3.23E-6 6 5.18E-6 0.24
8 SGTC_2803 7707263 4.03 71.4 µM 1.87E-5 4 2.80E-5 0.50
9 SGTC_2789 5281112 3.99 71.4 µM 2.22E-5 10 3.30E-5 0.07
10 SGTC_2049 5262831 3.89 67.2 µM 3.46E-5 7 5.05E-5 0.03
11 SGTC_1731 st035964 3.85 63.0 µM 4.12E-5 12 5.97E-5 0.03
12 SGTC_1118 chelidonine 3.82 707.5 nM 4.57E-5 2 6.60E-5 0.25
13 SGTC_932 2857-0803 3.76 154.0 µM 5.89E-5 8 8.42E-5 0.16
14 SGTC_2477 5967686 3.61 25.2 µM 1.10E-4 12 1.53E-4 0.18
15 SGTC_1528 arabinosylhypoxanthine 3.58 74.6 µM 1.23E-4 6 1.71E-4 0.07
16 SGTC_1076 itraconazole 3.55 331.0 nM 1.39E-4 8 1.92E-4 0.06
17 SGTC_2019 4016004 3.54 108.0 µM 1.45E-4 13 2.00E-4 0.02
18 SGTC_753 1322-0200 3.51 289.0 µM 1.62E-4 3 2.22E-4 0.12
19 SGTC_1593 justicidin b . 9-(1,3-benzodioxol-5-yl)- 6,7-dimethoxynaphtho[2,3-c]furan-1(3h)-one 3.48 54.9 µM 1.82E-4 redox potentiating 9 2.48E-4 0.16
20 SGTC_1787 fluconazole 3.36 80.0 µM 2.95E-4 iron homeostasis 17 3.96E-4 0.13

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.121 1.69E-12 YOL062C APM4 hom Mu2-like subunit of the clathrin associated protein complex (AP-2); involved in vesicle transport
0.101 4.90E-9 YLR416C_d YLR416C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.099 9.75E-9 YLL059C_d YLL059C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.097 1.61E-8 YAL062W GDH3 hom NADP(+)-dependent glutamate dehydrogenase; synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh1p; expression regulated by nitrogen and carbon sources; GDH3 has a paralog, GDH1, that arose from the whole genome duplication
0.094 4.22E-8 YGL047W ALG13 het Catalytic component of UDP-GlcNAc transferase; required for the second step of dolichyl-linked oligosaccharide synthesis; anchored to the ER membrane via interaction with Alg14p; similar to bacterial and human glycosyltransferases; protein abundance increases in response to DNA replication stress
0.093 6.24E-8 YDR037W KRS1 het Lysyl-tRNA synthetase
0.093 6.58E-8 YCL043C PDI1 het Protein disulfide isomerase; multifunctional protein of ER lumen, essential for formation of disulfide bonds in secretory and cell-surface proteins, unscrambles non-native disulfide bonds; key regulator of Ero1p; forms complex with Mnl1p that has exomannosidase activity, processing unfolded protein-bound Man8GlcNAc2 oligosaccharides to Man7GlcNAc2, promoting degradation in unfolded protein response; PDI1 has a paralog, EUG1, that arose from the whole genome duplication
0.084 1.16E-6 YCL025C AGP1 hom Low-affinity amino acid permease with broad substrate range; involved in uptake of asparagine, glutamine, and other amino acids; expression regulated by SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p); AGP1 has a paralog, GNP1, that arose from the whole genome duplication
0.082 1.72E-6 YKL122C SRP21 het Subunit of the signal recognition particle (SRP), which functions in protein targeting to the endoplasmic reticulum membrane; not found in mammalian SRP; forms a pre-SRP structure in the nucleolus that is translocated to the cytoplasm
0.079 4.07E-6 YPL190C NAB3 het RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3′ end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; required for termination of non-poly(A) transcripts and efficient splicing
0.079 4.67E-6 YOR304W ISW2 hom ATP-dependent DNA translocase involved in chromatin remodeling; ATPase component that, with Itc1p, forms a complex required for repression of a-specific genes, INO1, and early meiotic genes during mitotic growth
0.079 5.04E-6 YIR019C FLO11 hom GPI-anchored cell surface glycoprotein (flocculin); required for pseudohyphal formation, invasive growth, flocculation, and biofilms; transcriptionally regulated by the MAPK pathway (via Ste12p and Tec1p) and the cAMP pathway (via Flo8p); required for the formation of fibrous interconnections between cells in a colony of a wild S. cerevisiae strain
0.079 5.15E-6 YNR008W LRO1 hom Acyltransferase that catalyzes diacylglycerol esterification; one of several acyltransferases that contribute to triglyceride synthesis; putative homolog of human lecithin cholesterol acyltransferase
0.078 5.83E-6 YIL046W MET30 het F-box protein containing five copies of the WD40 motif, controls cell cycle function, sulfur metabolism, and methionine biosynthesis as part of the ubiquitin ligase complex; interacts with and regulates Met4p, localizes within the nucleus
0.078 6.23E-6 YNL161W CBK1 het Serine/threonine protein kinase of the the RAM signaling network; Ndr/LATS family member; binds regulatory subunit Mob2p; involved in regulation of cellular morphogenesis, polarized growth, and septum destruction; phosphorylation by Cbk1p regulates localization and activity of Ace2p transcription factor and Ssd1p translational repressor; Cbk1p activity is regulated by both phosphorylation and specific localization; relocalizes to cytoplasm upon DNA replication stress