YER169W / RPH1

JmjC domain-containing histone demethylase; specifically demethylates H3K36 tri- and dimethyl modification states; associates with actively transcribed (RNA polymerase II) regions in vivo and specifically targets H3K36 in its trimethylation state as its substrate; transcriptional repressor of PHR1; Rph1p phosphorylation during DNA damage is under control of the MEC1-RAD53 pathway; RPH1 has a paralog, GIS1, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YER169W / RPH1

Click on Significant Values for Screen Details ID:SGTC_258|Compound:haloperidol|FD-Score:4.09|P-value:5.73E-5 ID:SGTC_268|Compound:camptothecin|FD-Score:5.40|P-value:1.78E-7 ID:SGTC_271|Compound:rotenone|FD-Score:-3.38|P-value:7.29E-4 ID:SGTC_535|Compound:2293-4129|FD-Score:-3.61|P-value:3.35E-4 ID:SGTC_550|Compound:4123-0385|FD-Score:3.60|P-value:3.48E-4 ID:SGTC_679|Compound:0422-0091|FD-Score:3.74|P-value:2.14E-4 ID:SGTC_871|Compound:0574-0004|FD-Score:6.33|P-value:1.24E-9 ID:SGTC_882|Compound:0843-0131|FD-Score:-3.80|P-value:1.71E-4 ID:SGTC_919|Compound:tnp00227|FD-Score:4.78|P-value:3.32E-6 ID:SGTC_952|Compound:1185-0122|FD-Score:3.63|P-value:3.11E-4 ID:SGTC_1448|Compound:4255-0083|FD-Score:4.11|P-value:5.41E-5 ID:SGTC_1451|Compound:4152-0210|FD-Score:4.77|P-value:3.44E-6 ID:SGTC_1477|Compound:0139-0234|FD-Score:3.59|P-value:3.58E-4 ID:SGTC_1505|Compound:k297-0037|FD-Score:3.29|P-value:9.52E-4 ID:SGTC_1719|Compound:st032227|FD-Score:5.01|P-value:1.19E-6 ID:SGTC_1822|Compound:st052468|FD-Score:3.48|P-value:5.15E-4 ID:SGTC_1944|Compound:st076371|FD-Score:-3.34|P-value:8.12E-4 ID:SGTC_1945|Compound:st075991|FD-Score:-3.73|P-value:2.22E-4 ID:SGTC_2174|Compound:5790901|FD-Score:4.16|P-value:4.48E-5 ID:SGTC_2313|Compound:7726885|FD-Score:3.66|P-value:2.85E-4 ID:SGTC_2376|Compound:9072922|FD-Score:7.73|P-value:1.57E-13 ID:SGTC_2378|Compound:9074213|FD-Score:3.65|P-value:2.93E-4 ID:SGTC_2385|Compound:9074692|FD-Score:3.82|P-value:1.61E-4 ID:SGTC_2476|Compound:5806916|FD-Score:-3.62|P-value:3.22E-4 ID:SGTC_2500|Compound:5-alpha-androstan-3,17-dione|FD-Score:3.28|P-value:9.98E-4 ID:SGTC_2527|Compound:2',3'-dihydroxy-4-methoxy-4'-ethoxybenzophenone|FD-Score:-4.44|P-value:1.41E-5 ID:SGTC_2533|Compound:agelasine|FD-Score:3.36|P-value:7.69E-4 ID:SGTC_2638|Compound:epoxomicin|FD-Score:4.23|P-value:3.41E-5 ID:SGTC_2657|Compound:nystatin|FD-Score:3.94|P-value:1.03E-4 ID:SGTC_2767|Compound:sibutramine|FD-Score:-3.64|P-value:3.04E-4 ID:SGTC_2768|Compound:sulfasalazine|FD-Score:-4.42|P-value:1.53E-5 ID:SGTC_2771|Compound:fluoxetine|FD-Score:-3.37|P-value:7.54E-4 ID:SGTC_2774|Compound:tamoxifen|FD-Score:5.71|P-value:3.63E-8 ID:SGTC_2778|Compound:tioconazole|FD-Score:3.93|P-value:1.06E-4 ID:SGTC_2784|Compound:7567673|FD-Score:4.73|P-value:4.08E-6 ID:SGTC_2852|Compound:9023854|FD-Score:3.53|P-value:4.35E-4 ID:SGTC_2899|Compound:9053889|FD-Score:3.98|P-value:8.92E-5 ID:SGTC_2975|Compound:9084847|FD-Score:3.61|P-value:3.37E-4 ID:SGTC_3058|Compound:9093861|FD-Score:3.36|P-value:7.80E-4 ID:SGTC_3059|Compound:1327-0034|FD-Score:3.52|P-value:4.58E-4 ID:SGTC_3185|Compound:9108267|FD-Score:3.37|P-value:7.47E-4 ID:SGTC_3208|Compound:9114115|FD-Score:3.92|P-value:1.10E-4 ID:SGTC_3240|Compound:9133527|FD-Score:4.00|P-value:8.08E-5 ID:SGTC_258|Compound:haloperidol|FD-Score:4.09|P-value:5.73E-5 ID:SGTC_268|Compound:camptothecin|FD-Score:5.40|P-value:1.78E-7 ID:SGTC_271|Compound:rotenone|FD-Score:-3.38|P-value:7.29E-4 ID:SGTC_535|Compound:2293-4129|FD-Score:-3.61|P-value:3.35E-4 ID:SGTC_550|Compound:4123-0385|FD-Score:3.60|P-value:3.48E-4 ID:SGTC_679|Compound:0422-0091|FD-Score:3.74|P-value:2.14E-4 ID:SGTC_871|Compound:0574-0004|FD-Score:6.33|P-value:1.24E-9 ID:SGTC_882|Compound:0843-0131|FD-Score:-3.80|P-value:1.71E-4 ID:SGTC_919|Compound:tnp00227|FD-Score:4.78|P-value:3.32E-6 ID:SGTC_952|Compound:1185-0122|FD-Score:3.63|P-value:3.11E-4 ID:SGTC_1448|Compound:4255-0083|FD-Score:4.11|P-value:5.41E-5 ID:SGTC_1451|Compound:4152-0210|FD-Score:4.77|P-value:3.44E-6 ID:SGTC_1477|Compound:0139-0234|FD-Score:3.59|P-value:3.58E-4 ID:SGTC_1505|Compound:k297-0037|FD-Score:3.29|P-value:9.52E-4 ID:SGTC_1719|Compound:st032227|FD-Score:5.01|P-value:1.19E-6 ID:SGTC_1822|Compound:st052468|FD-Score:3.48|P-value:5.15E-4 ID:SGTC_1944|Compound:st076371|FD-Score:-3.34|P-value:8.12E-4 ID:SGTC_1945|Compound:st075991|FD-Score:-3.73|P-value:2.22E-4 ID:SGTC_2174|Compound:5790901|FD-Score:4.16|P-value:4.48E-5 ID:SGTC_2313|Compound:7726885|FD-Score:3.66|P-value:2.85E-4 ID:SGTC_2376|Compound:9072922|FD-Score:7.73|P-value:1.57E-13 ID:SGTC_2378|Compound:9074213|FD-Score:3.65|P-value:2.93E-4 ID:SGTC_2385|Compound:9074692|FD-Score:3.82|P-value:1.61E-4 ID:SGTC_2476|Compound:5806916|FD-Score:-3.62|P-value:3.22E-4 ID:SGTC_2500|Compound:5-alpha-androstan-3,17-dione|FD-Score:3.28|P-value:9.98E-4 ID:SGTC_2527|Compound:2',3'-dihydroxy-4-methoxy-4'-ethoxybenzophenone|FD-Score:-4.44|P-value:1.41E-5 ID:SGTC_2533|Compound:agelasine|FD-Score:3.36|P-value:7.69E-4 ID:SGTC_2638|Compound:epoxomicin|FD-Score:4.23|P-value:3.41E-5 ID:SGTC_2657|Compound:nystatin|FD-Score:3.94|P-value:1.03E-4 ID:SGTC_2767|Compound:sibutramine|FD-Score:-3.64|P-value:3.04E-4 ID:SGTC_2768|Compound:sulfasalazine|FD-Score:-4.42|P-value:1.53E-5 ID:SGTC_2771|Compound:fluoxetine|FD-Score:-3.37|P-value:7.54E-4 ID:SGTC_2774|Compound:tamoxifen|FD-Score:5.71|P-value:3.63E-8 ID:SGTC_2778|Compound:tioconazole|FD-Score:3.93|P-value:1.06E-4 ID:SGTC_2784|Compound:7567673|FD-Score:4.73|P-value:4.08E-6 ID:SGTC_2852|Compound:9023854|FD-Score:3.53|P-value:4.35E-4 ID:SGTC_2899|Compound:9053889|FD-Score:3.98|P-value:8.92E-5 ID:SGTC_2975|Compound:9084847|FD-Score:3.61|P-value:3.37E-4 ID:SGTC_3058|Compound:9093861|FD-Score:3.36|P-value:7.80E-4 ID:SGTC_3059|Compound:1327-0034|FD-Score:3.52|P-value:4.58E-4 ID:SGTC_3185|Compound:9108267|FD-Score:3.37|P-value:7.47E-4 ID:SGTC_3208|Compound:9114115|FD-Score:3.92|P-value:1.10E-4 ID:SGTC_3240|Compound:9133527|FD-Score:4.00|P-value:8.08E-5

Top fitness defect scores for YER169W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature		Screen
Rank
Screen
P-value
1 SGTC_2376 9072922 7.73 81.3 µM 1.57E-13 3 5.26E-15
2 SGTC_871 0574-0004 6.33 149.0 µM 1.24E-9 11 1.25E-10
3 SGTC_2774 tamoxifen 5.71 10.9 µM 3.63E-8 3 5.54E-9
4 SGTC_268 camptothecin 5.40 39.6 µM 1.78E-7 DNA damage response 39 3.29E-8
5 SGTC_1719 st032227 5.01 49.2 µM 1.19E-6 11 2.79E-7
6 SGTC_919 tnp00227 4.78 403.6 nM 3.32E-6 RNA pol III & RNase P/MRP 15 8.75E-7
7 SGTC_1451 4152-0210 4.77 104.0 µM 3.44E-6 10 9.12E-7
8 SGTC_2784 7567673 4.73 72.7 µM 4.08E-6 iron homeostasis 17 1.10E-6
9 SGTC_2638 epoxomicin 4.23 18.0 µM 3.41E-5 26 1.19E-5
10 SGTC_2174 5790901 4.16 200.0 µM 4.48E-5 tubulin folding & SWR complex 32 1.62E-5
11 SGTC_1448 4255-0083 4.11 152.0 µM 5.41E-5 ERG2 15 1.99E-5
12 SGTC_258 haloperidol 4.09 50.8 µM 5.73E-5 fatty acid desaturase (OLE1) 27 2.13E-5
13 SGTC_3240 9133527 4.00 49.5 µM 8.08E-5 10 3.12E-5
14 SGTC_2899 9053889 3.98 58.4 µM 8.92E-5 10 3.49E-5
15 SGTC_2657 nystatin 3.94 74.4 µM 1.03E-4 11 4.09E-5
16 SGTC_2778 tioconazole 3.93 65.1 nM 1.06E-4 ergosterol depletion effects on membrane 62 4.22E-5
17 SGTC_3208 9114115 3.92 49.5 µM 1.10E-4 36 4.39E-5
18 SGTC_2385 9074692 3.82 200.0 µM 1.61E-4 49 6.75E-5
19 SGTC_679 0422-0091 3.74 41.4 µM 2.14E-4 Golgi 38 9.27E-5
20 SGTC_2313 7726885 3.66 200.0 µM 2.85E-4 RPP1 & pyrimidine depletion 44 1.28E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.107 6.21E-10 YNL125C ESBP6 hom Protein with similarity to monocarboxylate permeases, appears not to be involved in transport of monocarboxylates such as lactate, pyruvate or acetate across the plasma membrane
0.101 4.63E-9 YDR217C RAD9 hom DNA damage-dependent checkpoint protein; required for cell-cycle arrest in G1/S, intra-S, and G2/M; transmits checkpoint signal by activating Rad53p and Chk1p; hyperphosphorylated by Mec1p and Tel1p; multiple cyclin dependent kinase consensus sites and the C-terminal BRCT domain contribute to DNA damage checkpoint activation; potential Cdc28p substrate
0.098 1.43E-8 YNL298W CLA4 hom Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p
0.096 2.47E-8 YDR413C_d YDR413C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the essential uncharacterized ORF YDR412W that is implicated in rRNA processing
0.092 1.04E-7 YPR009W SUT2 hom Putative transcription factor; multicopy suppressor of mutations that cause low activity of the cAMP/protein kinase A pathway; highly similar to Sut1p
0.089 2.33E-7 YPR122W AXL1 hom Haploid specific endoprotease that performs one of two N-terminal cleavages during maturation of a-factor mating pheromone; required for axial budding pattern of haploid cells
0.084 1.02E-6 YOR236W DFR1 het Dihydrofolate reductase involved in tetrahydrofolate biosynthesis; required for respiratory metabolism
0.083 1.35E-6 YGR047C TFC4 het One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA domain of TFIIIC that binds BoxA DNA promoter sites of tRNA and similar genes; has TPR motifs; human homolog is TFIIIC-102
0.082 2.05E-6 YER165W PAB1 het Poly(A) binding protein, part of the 3'-end RNA-processing complex, mediates interactions between the 5' cap structure and the 3' mRNA poly(A) tail, involved in control of poly(A) tail length, interacts with translation factor eIF-4G
0.081 2.28E-6 YJL216C IMA5 hom Alpha-glucosidase; specificity for isomaltose, maltose, and palatinose, but not alpha-methylglucoside; member of the IMA isomaltase family; not required for isomaltose utilization, but Ima5p overexpression allows the ima1 null mutant to grow on isomaltose
0.081 2.47E-6 YOR257W CDC31 het Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation
0.081 2.65E-6 YOL032W OPI10 hom Protein with a possible role in phospholipid biosynthesis; null mutant displays an inositol-excreting phenotype that is suppressed by exogenous choline; protein abundance increases in response to DNA replication stress
0.081 2.78E-6 YPR201W ARR3 hom Plasma membrane metalloid/H+ antiporter; transports arsenite and antimonite; required for resistance to arsenic compounds; transcription is activated by Arr1p in the presence of arsenite
0.079 4.85E-6 YER157W COG3 het Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
0.078 6.24E-6 YBR098W MMS4 hom Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in recombination, DNA repair, and joint molecule formation/resolution during meiotic recombination; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p