YER173W / RAD24

Checkpoint protein, involved in the activation of the DNA damage and meiotic pachytene checkpoints; subunit of a clamp loader that loads Rad17p-Mec3p-Ddc1p onto DNA; homolog of human and S. pombe Rad17 protein

Zygosity: Homozygous strain
fixedexpanded
Profile for YER173W / RAD24

Click on Significant Values for Screen Details ID:SGTC_119|Compound:0121-0043|FD-Score:3.94|P-value:6.39E-5 ID:SGTC_184|Compound:1494-0393|FD-Score:-3.77|P-value:1.15E-4 ID:SGTC_233|Compound:rapamycin|FD-Score:3.69|P-value:1.68E-4 ID:SGTC_268|Compound:camptothecin|FD-Score:3.94|P-value:6.35E-5 ID:SGTC_334|Compound:1502-1024|FD-Score:-3.75|P-value:1.24E-4 ID:SGTC_338|Compound:4055-0136|FD-Score:3.26|P-value:7.65E-4 ID:SGTC_462|Compound:ag-879|FD-Score:3.49|P-value:3.41E-4 ID:SGTC_573|Compound:3702-0553|FD-Score:4.41|P-value:8.77E-6 ID:SGTC_803|Compound:4488-1585|FD-Score:-3.36|P-value:5.13E-4 ID:SGTC_865|Compound:0488-0499|FD-Score:-3.50|P-value:3.07E-4 ID:SGTC_963|Compound:1269-2853|FD-Score:3.76|P-value:1.30E-4 ID:SGTC_1011|Compound:4112-3381|FD-Score:3.22|P-value:8.86E-4 ID:SGTC_1070|Compound:indatraline|FD-Score:4.38|P-value:1.01E-5 ID:SGTC_1197|Compound:1601-0112|FD-Score:4.23|P-value:1.92E-5 ID:SGTC_1433|Compound:4060-0023|FD-Score:3.80|P-value:1.11E-4 ID:SGTC_1437|Compound:4092-1038|FD-Score:3.30|P-value:6.72E-4 ID:SGTC_1493|Compound:4348-0038|FD-Score:-3.45|P-value:3.75E-4 ID:SGTC_1502|Compound:4534-4282|FD-Score:3.25|P-value:7.87E-4 ID:SGTC_1516|Compound:st013856|FD-Score:4.47|P-value:6.76E-6 ID:SGTC_1588|Compound:farnesol|FD-Score:3.39|P-value:4.92E-4 ID:SGTC_1664|Compound:st014217|FD-Score:3.20|P-value:9.42E-4 ID:SGTC_1948|Compound:st076329|FD-Score:-3.68|P-value:1.59E-4 ID:SGTC_1966|Compound:st070145|FD-Score:3.24|P-value:8.23E-4 ID:SGTC_2003|Compound:4023581|FD-Score:3.61|P-value:2.22E-4 ID:SGTC_2107|Compound:5363541|FD-Score:-3.35|P-value:5.24E-4 ID:SGTC_2120|Compound:5538279|FD-Score:3.72|P-value:1.49E-4 ID:SGTC_2246|Compound:7293507|FD-Score:-3.63|P-value:1.95E-4 ID:SGTC_2270|Compound:7924575|FD-Score:3.93|P-value:6.75E-5 ID:SGTC_2298|Compound:7976326|FD-Score:-4.94|P-value:7.01E-7 ID:SGTC_2507|Compound:purpurin|FD-Score:3.78|P-value:1.19E-4 ID:SGTC_2600|Compound:tangeritin|FD-Score:3.65|P-value:1.95E-4 ID:SGTC_2650|Compound:prasterone|FD-Score:-3.29|P-value:6.56E-4 ID:SGTC_2667|Compound:digitoxin|FD-Score:-4.43|P-value:7.61E-6 ID:SGTC_2686|Compound:alizarin|FD-Score:3.25|P-value:7.90E-4 ID:SGTC_2838|Compound:9002687|FD-Score:4.24|P-value:1.88E-5 ID:SGTC_2843|Compound:9000894|FD-Score:-4.29|P-value:1.35E-5 ID:SGTC_3102|Compound:9121669|FD-Score:3.19|P-value:9.64E-4 ID:SGTC_3217|Compound:9129673|FD-Score:3.29|P-value:6.83E-4 ID:SGTC_119|Compound:0121-0043|FD-Score:3.94|P-value:6.39E-5 ID:SGTC_184|Compound:1494-0393|FD-Score:-3.77|P-value:1.15E-4 ID:SGTC_233|Compound:rapamycin|FD-Score:3.69|P-value:1.68E-4 ID:SGTC_268|Compound:camptothecin|FD-Score:3.94|P-value:6.35E-5 ID:SGTC_334|Compound:1502-1024|FD-Score:-3.75|P-value:1.24E-4 ID:SGTC_338|Compound:4055-0136|FD-Score:3.26|P-value:7.65E-4 ID:SGTC_462|Compound:ag-879|FD-Score:3.49|P-value:3.41E-4 ID:SGTC_573|Compound:3702-0553|FD-Score:4.41|P-value:8.77E-6 ID:SGTC_803|Compound:4488-1585|FD-Score:-3.36|P-value:5.13E-4 ID:SGTC_865|Compound:0488-0499|FD-Score:-3.50|P-value:3.07E-4 ID:SGTC_963|Compound:1269-2853|FD-Score:3.76|P-value:1.30E-4 ID:SGTC_1011|Compound:4112-3381|FD-Score:3.22|P-value:8.86E-4 ID:SGTC_1070|Compound:indatraline|FD-Score:4.38|P-value:1.01E-5 ID:SGTC_1197|Compound:1601-0112|FD-Score:4.23|P-value:1.92E-5 ID:SGTC_1433|Compound:4060-0023|FD-Score:3.80|P-value:1.11E-4 ID:SGTC_1437|Compound:4092-1038|FD-Score:3.30|P-value:6.72E-4 ID:SGTC_1493|Compound:4348-0038|FD-Score:-3.45|P-value:3.75E-4 ID:SGTC_1502|Compound:4534-4282|FD-Score:3.25|P-value:7.87E-4 ID:SGTC_1516|Compound:st013856|FD-Score:4.47|P-value:6.76E-6 ID:SGTC_1588|Compound:farnesol|FD-Score:3.39|P-value:4.92E-4 ID:SGTC_1664|Compound:st014217|FD-Score:3.20|P-value:9.42E-4 ID:SGTC_1948|Compound:st076329|FD-Score:-3.68|P-value:1.59E-4 ID:SGTC_1966|Compound:st070145|FD-Score:3.24|P-value:8.23E-4 ID:SGTC_2003|Compound:4023581|FD-Score:3.61|P-value:2.22E-4 ID:SGTC_2107|Compound:5363541|FD-Score:-3.35|P-value:5.24E-4 ID:SGTC_2120|Compound:5538279|FD-Score:3.72|P-value:1.49E-4 ID:SGTC_2246|Compound:7293507|FD-Score:-3.63|P-value:1.95E-4 ID:SGTC_2270|Compound:7924575|FD-Score:3.93|P-value:6.75E-5 ID:SGTC_2298|Compound:7976326|FD-Score:-4.94|P-value:7.01E-7 ID:SGTC_2507|Compound:purpurin|FD-Score:3.78|P-value:1.19E-4 ID:SGTC_2600|Compound:tangeritin|FD-Score:3.65|P-value:1.95E-4 ID:SGTC_2650|Compound:prasterone|FD-Score:-3.29|P-value:6.56E-4 ID:SGTC_2667|Compound:digitoxin|FD-Score:-4.43|P-value:7.61E-6 ID:SGTC_2686|Compound:alizarin|FD-Score:3.25|P-value:7.90E-4 ID:SGTC_2838|Compound:9002687|FD-Score:4.24|P-value:1.88E-5 ID:SGTC_2843|Compound:9000894|FD-Score:-4.29|P-value:1.35E-5 ID:SGTC_3102|Compound:9121669|FD-Score:3.19|P-value:9.64E-4 ID:SGTC_3217|Compound:9129673|FD-Score:3.29|P-value:6.83E-4

Top fitness defect scores for YER173W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1516 st013856 4.47 110.0 µM 6.76E-6 7 3.84E-6
2 SGTC_573 3702-0553 4.41 84.7 µM 8.77E-6 7 5.05E-6
3 SGTC_1070 indatraline 4.38 8.8 µM 1.01E-5 60S ribosome export 17 5.88E-6
4 SGTC_2838 9002687 4.24 71.4 µM 1.88E-5 fatty acid desaturase (OLE1) 18 1.13E-5
5 SGTC_1197 1601-0112 4.23 267.0 µM 1.92E-5 14 1.15E-5
6 SGTC_268 camptothecin 3.94 39.6 µM 6.35E-5 DNA damage response 74 4.06E-5
7 SGTC_119 0121-0043 3.94 24.8 µM 6.39E-5 mitochondrial stress 113 4.08E-5
8 SGTC_2270 7924575 3.93 90.9 µM 6.75E-5 RPP1 & pyrimidine depletion 16 4.32E-5
9 SGTC_1433 4060-0023 3.80 7.6 µM 1.11E-4 tubulin folding & SWR complex 23 7.30E-5
10 SGTC_2507 purpurin 3.78 79.7 µM 1.19E-4 copper-dependent oxidative stress 44 7.87E-5
11 SGTC_963 1269-2853 3.76 151.0 µM 1.30E-4 31 8.61E-5
12 SGTC_2120 5538279 3.72 152.0 µM 1.49E-4 23 9.98E-5
13 SGTC_233 rapamycin 3.69 362.0 pM 1.68E-4 16 1.13E-4
14 SGTC_2600 tangeritin 3.65 88.0 µM 1.95E-4 46 1.32E-4
15 SGTC_2003 4023581 3.61 200.0 µM 2.22E-4 cell wall signaling 41 1.51E-4
16 SGTC_462 ag-879 3.49 158.0 µM 3.41E-4 47 2.38E-4
17 SGTC_1588 farnesol 3.39 29.9 µM 4.92E-4 23 3.49E-4
18 SGTC_1437 4092-1038 3.30 11.6 µM 6.72E-4 35 4.84E-4
19 SGTC_3217 9129673 3.29 49.5 µM 6.83E-4 68 4.93E-4
20 SGTC_338 4055-0136 3.26 114.0 µM 7.65E-4 25 5.55E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.114 3.55E-11 YJL216C IMA5 hom Alpha-glucosidase; specificity for isomaltose, maltose, and palatinose, but not alpha-methylglucoside; member of the IMA isomaltase family; not required for isomaltose utilization, but Ima5p overexpression allows the ima1 null mutant to grow on isomaltose
0.109 2.63E-10 YNR073C_p YNR073C_p hom Putative mannitol dehydrogenase; identical in amino acid sequence to Dsf1p
0.101 4.54E-9 YMR026C PEX12 hom C3HC4-type RING-finger peroxin and E3 ubiquitin ligase, required for peroxisome biogenesis and peroxisomal matrix protein import; forms translocation subcomplex with Pex2p and Pex10p; mutations in human homolog cause peroxisomal disorder
0.097 1.56E-8 YPL274W SAM3 hom High-affinity S-adenosylmethionine permease, required for utilization of S-adenosylmethionine as a sulfur source; has similarity to S-methylmethionine permease Mmp1p
0.094 5.00E-8 YDR217C RAD9 hom DNA damage-dependent checkpoint protein; required for cell-cycle arrest in G1/S, intra-S, and G2/M; transmits checkpoint signal by activating Rad53p and Chk1p; hyperphosphorylated by Mec1p and Tel1p; multiple cyclin dependent kinase consensus sites and the C-terminal BRCT domain contribute to DNA damage checkpoint activation; potential Cdc28p substrate
0.083 1.33E-6 YFR001W LOC1 hom Nuclear protein involved in asymmetric localization of ASH1 mRNA; binds double-stranded RNA in vitro; constituent of 66S pre-ribosomal particles; relocalizes from nucleus to cytoplasm upon DNA replication stress
0.081 2.58E-6 YNL173C MDG1 hom Plasma membrane protein involved in G-protein mediated pheromone signaling pathway; overproduction suppresses bem1 mutations
0.080 3.43E-6 YBR158W AMN1 hom Protein required for daughter cell separation, multiple mitotic checkpoints, and chromosome stability; contains 12 degenerate leucine-rich repeat motifs; expression is induced by the Mitotic Exit Network (MEN)
0.075 1.21E-5 YDR452W PPN1 hom Vacuolar endopolyphosphatase with a role in phosphate metabolism; functions as a homodimer; relocalizes from vacuole to cytoplasm upon DNA replication stress
0.075 1.52E-5 YKL215C OXP1 hom 5-oxoprolinase; enzyme is ATP-dependent and functions as a dimer; similar to mouse Oplah gene; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress
0.074 1.79E-5 YGR110W CLD1 hom Mitochondrial cardiolipin-specific phospholipase; functions upstream of Taz1p to generate monolyso-cardiolipin; transcription increases upon genotoxic stress; involved in restricting Ty1 transposition; has homology to mammalian CGI-58
0.074 1.87E-5 YIR020W-A_d YIR020W-A_d hom Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data
0.073 2.28E-5 YLR459W GAB1 het GPI transamidase subunit, involved in attachment of glycosylphosphatidylinositol (GPI) anchors to proteins; may have a role in recognition of the attachment signal or of the lipid portion of GPI
0.070 4.55E-5 YPR013C_p CMR3_p hom Putative zinc finger protein; YPR013C is not an essential gene
0.069 6.86E-5 YPR145W ASN1 hom Asparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN1 has a paralog, ASN2, that arose from the whole genome duplication