YER174C / GRX4

Hydroperoxide and superoxide-radical responsive glutathione-dependent oxidoreductase; monothiol glutaredoxin subfamily member along with Grx3p and Grx5p; protects cells from oxidative damage; mutant has increased aneuploidy tolerance; transcription is regulated by Yap5

Zygosity: Homozygous strain
fixedexpanded
Profile for YER174C / GRX4

Click on Significant Values for Screen Details ID:SGTC_28|Compound:pentachloropyridine N-oxide|FD-Score:-3.10|P-value:9.81E-4 ID:SGTC_44|Compound:0929-0055|FD-Score:-5.44|P-value:2.88E-8 ID:SGTC_259|Compound:usnic acid|FD-Score:3.21|P-value:6.99E-4 ID:SGTC_300|Compound:r073-0011|FD-Score:3.52|P-value:2.27E-4 ID:SGTC_571|Compound:k831-0106|FD-Score:-3.62|P-value:1.53E-4 ID:SGTC_710|Compound:3448-4187|FD-Score:3.37|P-value:3.85E-4 ID:SGTC_831|Compound:k061-1017|FD-Score:3.92|P-value:4.63E-5 ID:SGTC_862|Compound:0452-0056|FD-Score:3.58|P-value:1.82E-4 ID:SGTC_912|Compound:3474-0017|FD-Score:4.02|P-value:3.05E-5 ID:SGTC_981|Compound:1488-1094|FD-Score:3.23|P-value:6.40E-4 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:3.16|P-value:8.31E-4 ID:SGTC_1131|Compound:3454-3128|FD-Score:4.47|P-value:4.28E-6 ID:SGTC_1133|Compound:3473-0247|FD-Score:-3.87|P-value:5.76E-5 ID:SGTC_1175|Compound:0741-0056|FD-Score:3.60|P-value:1.70E-4 ID:SGTC_1193|Compound:1488-0126|FD-Score:3.41|P-value:3.35E-4 ID:SGTC_1339|Compound:1486-1321|FD-Score:-3.85|P-value:6.29E-5 ID:SGTC_1448|Compound:4255-0083|FD-Score:3.60|P-value:1.68E-4 ID:SGTC_1454|Compound:4239-0054|FD-Score:4.86|P-value:6.36E-7 ID:SGTC_1542|Compound:7-hydroxy-3'-methoxyflavone|FD-Score:4.27|P-value:1.05E-5 ID:SGTC_1611|Compound:st001856|FD-Score:3.54|P-value:2.12E-4 ID:SGTC_1614|Compound:st002383|FD-Score:3.21|P-value:6.87E-4 ID:SGTC_1619|Compound:st003326|FD-Score:-3.15|P-value:8.44E-4 ID:SGTC_1677|Compound:st019204|FD-Score:3.14|P-value:8.85E-4 ID:SGTC_1748|Compound:st037455|FD-Score:-3.64|P-value:1.40E-4 ID:SGTC_1796|Compound:st049173|FD-Score:-3.50|P-value:2.39E-4 ID:SGTC_2026|Compound:5148700|FD-Score:3.52|P-value:2.22E-4 ID:SGTC_2033|Compound:5192550|FD-Score:3.39|P-value:3.64E-4 ID:SGTC_2243|Compound:6634756|FD-Score:-3.34|P-value:4.35E-4 ID:SGTC_2265|Compound:7929201|FD-Score:3.29|P-value:5.13E-4 ID:SGTC_2364|Compound:9055074|FD-Score:3.25|P-value:5.99E-4 ID:SGTC_2365|Compound:9042475|FD-Score:4.27|P-value:1.05E-5 ID:SGTC_2499|Compound:avocadenofuran|FD-Score:3.21|P-value:6.93E-4 ID:SGTC_2632|Compound:3-hydroxy-4-(succin-2-yl)-caryolane delta-lactone|FD-Score:3.24|P-value:6.30E-4 ID:SGTC_2802|Compound:7925815|FD-Score:3.56|P-value:1.91E-4 ID:SGTC_2878|Compound:9043973|FD-Score:3.49|P-value:2.51E-4 ID:SGTC_3188|Compound:9110789|FD-Score:3.69|P-value:1.19E-4 ID:SGTC_3211|Compound:9128781|FD-Score:3.24|P-value:6.27E-4 ID:SGTC_28|Compound:pentachloropyridine N-oxide|FD-Score:-3.10|P-value:9.81E-4 ID:SGTC_44|Compound:0929-0055|FD-Score:-5.44|P-value:2.88E-8 ID:SGTC_259|Compound:usnic acid|FD-Score:3.21|P-value:6.99E-4 ID:SGTC_300|Compound:r073-0011|FD-Score:3.52|P-value:2.27E-4 ID:SGTC_571|Compound:k831-0106|FD-Score:-3.62|P-value:1.53E-4 ID:SGTC_710|Compound:3448-4187|FD-Score:3.37|P-value:3.85E-4 ID:SGTC_831|Compound:k061-1017|FD-Score:3.92|P-value:4.63E-5 ID:SGTC_862|Compound:0452-0056|FD-Score:3.58|P-value:1.82E-4 ID:SGTC_912|Compound:3474-0017|FD-Score:4.02|P-value:3.05E-5 ID:SGTC_981|Compound:1488-1094|FD-Score:3.23|P-value:6.40E-4 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:3.16|P-value:8.31E-4 ID:SGTC_1131|Compound:3454-3128|FD-Score:4.47|P-value:4.28E-6 ID:SGTC_1133|Compound:3473-0247|FD-Score:-3.87|P-value:5.76E-5 ID:SGTC_1175|Compound:0741-0056|FD-Score:3.60|P-value:1.70E-4 ID:SGTC_1193|Compound:1488-0126|FD-Score:3.41|P-value:3.35E-4 ID:SGTC_1339|Compound:1486-1321|FD-Score:-3.85|P-value:6.29E-5 ID:SGTC_1448|Compound:4255-0083|FD-Score:3.60|P-value:1.68E-4 ID:SGTC_1454|Compound:4239-0054|FD-Score:4.86|P-value:6.36E-7 ID:SGTC_1542|Compound:7-hydroxy-3'-methoxyflavone|FD-Score:4.27|P-value:1.05E-5 ID:SGTC_1611|Compound:st001856|FD-Score:3.54|P-value:2.12E-4 ID:SGTC_1614|Compound:st002383|FD-Score:3.21|P-value:6.87E-4 ID:SGTC_1619|Compound:st003326|FD-Score:-3.15|P-value:8.44E-4 ID:SGTC_1677|Compound:st019204|FD-Score:3.14|P-value:8.85E-4 ID:SGTC_1748|Compound:st037455|FD-Score:-3.64|P-value:1.40E-4 ID:SGTC_1796|Compound:st049173|FD-Score:-3.50|P-value:2.39E-4 ID:SGTC_2026|Compound:5148700|FD-Score:3.52|P-value:2.22E-4 ID:SGTC_2033|Compound:5192550|FD-Score:3.39|P-value:3.64E-4 ID:SGTC_2243|Compound:6634756|FD-Score:-3.34|P-value:4.35E-4 ID:SGTC_2265|Compound:7929201|FD-Score:3.29|P-value:5.13E-4 ID:SGTC_2364|Compound:9055074|FD-Score:3.25|P-value:5.99E-4 ID:SGTC_2365|Compound:9042475|FD-Score:4.27|P-value:1.05E-5 ID:SGTC_2499|Compound:avocadenofuran|FD-Score:3.21|P-value:6.93E-4 ID:SGTC_2632|Compound:3-hydroxy-4-(succin-2-yl)-caryolane delta-lactone|FD-Score:3.24|P-value:6.30E-4 ID:SGTC_2802|Compound:7925815|FD-Score:3.56|P-value:1.91E-4 ID:SGTC_2878|Compound:9043973|FD-Score:3.49|P-value:2.51E-4 ID:SGTC_3188|Compound:9110789|FD-Score:3.69|P-value:1.19E-4 ID:SGTC_3211|Compound:9128781|FD-Score:3.24|P-value:6.27E-4

Top fitness defect scores for YER174C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1454 4239-0054 4.86 42.6 µM 6.36E-7 12 5.88E-7
2 SGTC_1131 3454-3128 4.47 32.3 µM 4.28E-6 23 4.00E-6
3 SGTC_1542 7-hydroxy-3'-methoxyflavone 4.27 49.7 µM 1.05E-5 9 9.85E-6
4 SGTC_2365 9042475 4.27 200.0 µM 1.05E-5 24 9.90E-6
5 SGTC_912 3474-0017 4.02 2.2 µM 3.05E-5 20 2.89E-5
6 SGTC_831 k061-1017 3.92 217.0 µM 4.63E-5 translation 65 4.39E-5
7 SGTC_3188 9110789 3.69 49.5 µM 1.19E-4 iron homeostasis 30 1.13E-4
8 SGTC_1448 4255-0083 3.60 152.0 µM 1.68E-4 ERG2 26 1.61E-4
9 SGTC_1175 0741-0056 3.60 27.6 µM 1.70E-4 32 1.62E-4
10 SGTC_862 0452-0056 3.58 10.2 µM 1.82E-4 azole & statin 40 1.74E-4
11 SGTC_2802 7925815 3.56 54.5 µM 1.91E-4 54 1.83E-4
12 SGTC_1611 st001856 3.54 99.4 µM 2.12E-4 27 2.03E-4
13 SGTC_2026 5148700 3.52 152.0 µM 2.22E-4 29 2.12E-4
14 SGTC_300 r073-0011 3.52 5.0 µM 2.27E-4 34 2.18E-4
15 SGTC_2878 9043973 3.49 26.0 µM 2.51E-4 32 2.40E-4
16 SGTC_1193 1488-0126 3.41 41.5 µM 3.35E-4 44 3.21E-4
17 SGTC_2033 5192550 3.39 145.0 µM 3.64E-4 28 3.49E-4
18 SGTC_710 3448-4187 3.37 58.0 µM 3.85E-4 fatty acid desaturase (OLE1) 86 3.70E-4
19 SGTC_2265 7929201 3.29 200.0 µM 5.13E-4 48 4.94E-4
20 SGTC_2364 9055074 3.25 200.0 µM 5.99E-4 42 5.77E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.146 1.93E-17 YGL090W LIF1 hom Component of the DNA ligase IV complex that mediates nonhomologous end joining in DNA double-strand break repair; physically interacts with Dnl4p and Nej1p; homologous to mammalian XRCC4 protein
0.138 1.26E-15 YHL045W_d YHL045W_d hom Putative protein of unknown function; not an essential gene
0.129 5.39E-14 YPL251W_d YPL251W_d het Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified, essential gene YAH1/YPL252C
0.119 4.44E-12 YKL132C RMA1 hom Putative dihydrofolate synthetase; has similarity to Fol3p and to E. coli folylpolyglutamate synthetase/dihydrofolate synthetase; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.111 1.16E-10 YBR026C ETR1 hom 2-enoyl thioester reductase, member of the medium chain dehydrogenase/reductase family; localized to in mitochondria, where it has a probable role in fatty acid synthesis
0.109 2.22E-10 YPL252C YAH1 het Ferredoxin of the mitochondrial matrix required for formation of cellular iron-sulfur proteins; involved in heme A biosynthesis; homologous to human adrenodoxin
0.098 1.39E-8 YLR264C-A_p YLR264C-A_p hom Putative protein of unknown function
0.090 1.61E-7 YGR243W FMP43 hom Highly conserved subunit of the mitochondrial pyruvate carrier; a mitochondrial inner membrane complex comprised of Fmp37p/Mpc1p and either Mpc2p or Fmp43p/Mpc3p mediates mitochondrial pyruvate uptake; more highly expressed in glucose-containing minimal medium than in lactate-containing medium; expression regulated by osmotic and alkaline stresses; protein abundance increases in response to DNA replication stress
0.089 2.08E-7 YKL143W LTV1 hom Component of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; required for ribosomal small subunit export from nucleus; required for growth at low temperature
0.089 2.14E-7 YDR431W_d YDR431W_d hom Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.088 3.03E-7 YOR014W RTS1 hom B-type regulatory subunit of protein phosphatase 2A (PP2A); Rts1p and Cdc55p are alternative regulatory subunits for PP2A catalytic subunits, Pph21p and Pph22p; PP2A-Rts1p protects cohesin when recruited by Sgo1p to the pericentromere; highly enriched at centromeres in the absence of Cdc55p; required for maintenance of septin ring organization during cytokinesis, for ring disassembly in G1 and for dephosphorylation of septin, Shs1p; homolog of the mammalian B' subunit of PP2A
0.086 6.08E-7 YLL010C PSR1 hom Plasma membrane associated protein phosphatase involved in the general stress response; required along with binding partner Whi2p for full activation of STRE-mediated gene expression, possibly through dephosphorylation of Msn2p
0.083 1.43E-6 YOR360C PDE2 hom High-affinity cyclic AMP phosphodiesterase, component of the cAMP-dependent protein kinase signaling system, protects the cell from extracellular cAMP, contains readthrough motif surrounding termination codon
0.083 1.48E-6 YMR142C RPL13B hom Ribosomal 60S subunit protein L13B; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13B has a paralog, RPL13A, that arose from the whole genome duplication
0.083 1.49E-6 YER069W ARG5,6 hom Acetylglutamate kinase and N-acetyl-gamma-glutamyl-phosphate reductase; N-acetyl-L-glutamate kinase (NAGK) catalyzes the 2nd and N-acetyl-gamma-glutamyl-phosphate reductase (NAGSA), the 3rd step in arginine biosynthesis; synthesized as a precursor which is processed in the mitochondrion to yield mature NAGK and NAGSA; enzymes form a metabolon complex with Arg2p; NAGK C-terminal domain stabilizes the enzymes, slows catalysis and is involved in feed-back inhibition by arginine