YER177W / BMH1

14-3-3 protein, major isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YER177W / BMH1

Click on Significant Values for Screen Details ID:SGTC_65|Compound:0798-0905|FD-Score:7.28|P-value:5.78E-12 ID:SGTC_97|Compound:0535-0701|FD-Score:4.62|P-value:7.65E-6 ID:SGTC_221|Compound:trifluoperazine|FD-Score:7.22|P-value:8.51E-12 ID:SGTC_227|Compound:fluconazole|FD-Score:7.45|P-value:1.84E-12 ID:SGTC_256|Compound:clotrimazole|FD-Score:4.45|P-value:1.51E-5 ID:SGTC_304|Compound:1310-0298|FD-Score:-3.45|P-value:8.28E-4 ID:SGTC_314|Compound:1671-0008|FD-Score:3.57|P-value:4.01E-4 ID:SGTC_363|Compound:0455-0021|FD-Score:3.60|P-value:3.62E-4 ID:SGTC_410|Compound:α-linolenic acid|FD-Score:3.71|P-value:2.47E-4 ID:SGTC_422|Compound:cantharidin|FD-Score:3.71|P-value:2.45E-4 ID:SGTC_491|Compound:tetrandrine|FD-Score:3.84|P-value:1.54E-4 ID:SGTC_526|Compound:4469-0568|FD-Score:5.96|P-value:1.31E-8 ID:SGTC_547|Compound:1120-0019|FD-Score:4.05|P-value:7.26E-5 ID:SGTC_558|Compound:k064-0027|FD-Score:4.13|P-value:5.36E-5 ID:SGTC_581|Compound:0958-0271|FD-Score:-4.53|P-value:1.70E-5 ID:SGTC_658|Compound:3253-0951|FD-Score:3.46|P-value:5.73E-4 ID:SGTC_747|Compound:3448-8087|FD-Score:5.25|P-value:4.62E-7 ID:SGTC_822|Compound:1487-0950|FD-Score:3.52|P-value:4.67E-4 ID:SGTC_887|Compound:0885-0007|FD-Score:3.95|P-value:1.04E-4 ID:SGTC_902|Compound:1120-0002|FD-Score:6.30|P-value:2.05E-9 ID:SGTC_917|Compound:harmaline|FD-Score:4.35|P-value:2.27E-5 ID:SGTC_1067|Compound:clotrimazole|FD-Score:8.28|P-value:5.89E-15 ID:SGTC_1074|Compound:carmofur|FD-Score:3.80|P-value:1.77E-4 ID:SGTC_1084|Compound:voriconazole|FD-Score:5.75|P-value:3.93E-8 ID:SGTC_1338|Compound:1486-1223|FD-Score:3.99|P-value:9.05E-5 ID:SGTC_1368|Compound:1852-0141|FD-Score:3.91|P-value:1.23E-4 ID:SGTC_1445|Compound:4239-0156|FD-Score:-3.90|P-value:1.88E-4 ID:SGTC_1495|Compound:4469-0029|FD-Score:3.58|P-value:3.83E-4 ID:SGTC_1503|Compound:4585-0012|FD-Score:3.57|P-value:3.99E-4 ID:SGTC_1547|Compound:harmine|FD-Score:3.78|P-value:1.91E-4 ID:SGTC_1599|Compound:8-chlro-1-tetrahydronorharmanone|FD-Score:4.89|P-value:2.42E-6 ID:SGTC_1628|Compound:st004252|FD-Score:3.43|P-value:6.25E-4 ID:SGTC_1636|Compound:st007707|FD-Score:3.64|P-value:3.16E-4 ID:SGTC_1745|Compound:st081495|FD-Score:6.04|P-value:8.42E-9 ID:SGTC_1754|Compound:st042065|FD-Score:8.59|P-value:6.14E-16 ID:SGTC_1759|Compound:st043059|FD-Score:-4.05|P-value:1.08E-4 ID:SGTC_1788|Compound:fluconazole|FD-Score:4.64|P-value:6.85E-6 ID:SGTC_1800|Compound:st050079|FD-Score:4.79|P-value:3.71E-6 ID:SGTC_1802|Compound:st050112|FD-Score:3.57|P-value:3.91E-4 ID:SGTC_1803|Compound:st050068|FD-Score:7.72|P-value:3.01E-13 ID:SGTC_1814|Compound:st052110|FD-Score:-4.14|P-value:7.82E-5 ID:SGTC_1818|Compound:st052136|FD-Score:6.24|P-value:2.88E-9 ID:SGTC_1844|Compound:rapamycin|FD-Score:4.35|P-value:2.25E-5 ID:SGTC_1876|Compound:myriocin|FD-Score:6.71|P-value:1.96E-10 ID:SGTC_1970|Compound:st070122|FD-Score:-4.11|P-value:8.67E-5 ID:SGTC_2021|Compound:4019055|FD-Score:3.39|P-value:7.26E-4 ID:SGTC_2057|Compound:5236571|FD-Score:3.48|P-value:5.37E-4 ID:SGTC_2089|Compound:5281248|FD-Score:3.44|P-value:6.08E-4 ID:SGTC_2248|Compound:tofa|FD-Score:11.00|P-value:5.72E-25 ID:SGTC_2264|Compound:7948757|FD-Score:3.70|P-value:2.53E-4 ID:SGTC_2283|Compound:7944793|FD-Score:3.93|P-value:1.13E-4 ID:SGTC_2309|Compound:7664088|FD-Score:3.32|P-value:8.89E-4 ID:SGTC_2331|Compound:7980721|FD-Score:3.66|P-value:2.92E-4 ID:SGTC_2397|Compound:5161689|FD-Score:3.61|P-value:3.50E-4 ID:SGTC_2416|Compound:tofa|FD-Score:17.00|P-value:4.03E-56 ID:SGTC_2495|Compound:cafestol|FD-Score:-3.65|P-value:4.41E-4 ID:SGTC_2591|Compound:skatole|FD-Score:3.42|P-value:6.60E-4 ID:SGTC_2623|Compound:humulene|FD-Score:-3.72|P-value:3.47E-4 ID:SGTC_2645|Compound:abamectin|FD-Score:4.07|P-value:6.83E-5 ID:SGTC_2659|Compound:sanguinarine|FD-Score:3.41|P-value:6.62E-4 ID:SGTC_2665|Compound:lovastatin|FD-Score:3.60|P-value:3.65E-4 ID:SGTC_2679|Compound:rapamycin|FD-Score:5.84|P-value:2.48E-8 ID:SGTC_2688|Compound:kanamycin b|FD-Score:6.65|P-value:2.71E-10 ID:SGTC_2765|Compound:myclobutanil|FD-Score:5.74|P-value:4.16E-8 ID:SGTC_2778|Compound:tioconazole|FD-Score:4.35|P-value:2.27E-5 ID:SGTC_2816|Compound:7982706|FD-Score:5.09|P-value:9.56E-7 ID:SGTC_2823|Compound:7987624|FD-Score:-3.97|P-value:1.47E-4 ID:SGTC_2830|Compound:7992007|FD-Score:3.35|P-value:8.25E-4 ID:SGTC_2970|Compound:9090938|FD-Score:3.34|P-value:8.41E-4 ID:SGTC_3043|Compound:9092303|FD-Score:-3.41|P-value:9.44E-4 ID:SGTC_3152|Compound:9099253|FD-Score:3.92|P-value:1.17E-4 ID:SGTC_3196|Compound:9111891|FD-Score:-4.13|P-value:8.11E-5 ID:SGTC_3197|Compound:9113331|FD-Score:-5.13|P-value:1.31E-6 ID:SGTC_3306|Compound:9127676|FD-Score:-3.62|P-value:4.79E-4 ID:SGTC_3308|Compound:9128332|FD-Score:3.41|P-value:6.68E-4 ID:SGTC_3339|Compound:9145883|FD-Score:4.01|P-value:8.49E-5 ID:SGTC_3354|Compound:9151612|FD-Score:3.82|P-value:1.67E-4 ID:SGTC_65|Compound:0798-0905|FD-Score:7.28|P-value:5.78E-12 ID:SGTC_97|Compound:0535-0701|FD-Score:4.62|P-value:7.65E-6 ID:SGTC_221|Compound:trifluoperazine|FD-Score:7.22|P-value:8.51E-12 ID:SGTC_227|Compound:fluconazole|FD-Score:7.45|P-value:1.84E-12 ID:SGTC_256|Compound:clotrimazole|FD-Score:4.45|P-value:1.51E-5 ID:SGTC_304|Compound:1310-0298|FD-Score:-3.45|P-value:8.28E-4 ID:SGTC_314|Compound:1671-0008|FD-Score:3.57|P-value:4.01E-4 ID:SGTC_363|Compound:0455-0021|FD-Score:3.60|P-value:3.62E-4 ID:SGTC_410|Compound:α-linolenic acid|FD-Score:3.71|P-value:2.47E-4 ID:SGTC_422|Compound:cantharidin|FD-Score:3.71|P-value:2.45E-4 ID:SGTC_491|Compound:tetrandrine|FD-Score:3.84|P-value:1.54E-4 ID:SGTC_526|Compound:4469-0568|FD-Score:5.96|P-value:1.31E-8 ID:SGTC_547|Compound:1120-0019|FD-Score:4.05|P-value:7.26E-5 ID:SGTC_558|Compound:k064-0027|FD-Score:4.13|P-value:5.36E-5 ID:SGTC_581|Compound:0958-0271|FD-Score:-4.53|P-value:1.70E-5 ID:SGTC_658|Compound:3253-0951|FD-Score:3.46|P-value:5.73E-4 ID:SGTC_747|Compound:3448-8087|FD-Score:5.25|P-value:4.62E-7 ID:SGTC_822|Compound:1487-0950|FD-Score:3.52|P-value:4.67E-4 ID:SGTC_887|Compound:0885-0007|FD-Score:3.95|P-value:1.04E-4 ID:SGTC_902|Compound:1120-0002|FD-Score:6.30|P-value:2.05E-9 ID:SGTC_917|Compound:harmaline|FD-Score:4.35|P-value:2.27E-5 ID:SGTC_1067|Compound:clotrimazole|FD-Score:8.28|P-value:5.89E-15 ID:SGTC_1074|Compound:carmofur|FD-Score:3.80|P-value:1.77E-4 ID:SGTC_1084|Compound:voriconazole|FD-Score:5.75|P-value:3.93E-8 ID:SGTC_1338|Compound:1486-1223|FD-Score:3.99|P-value:9.05E-5 ID:SGTC_1368|Compound:1852-0141|FD-Score:3.91|P-value:1.23E-4 ID:SGTC_1445|Compound:4239-0156|FD-Score:-3.90|P-value:1.88E-4 ID:SGTC_1495|Compound:4469-0029|FD-Score:3.58|P-value:3.83E-4 ID:SGTC_1503|Compound:4585-0012|FD-Score:3.57|P-value:3.99E-4 ID:SGTC_1547|Compound:harmine|FD-Score:3.78|P-value:1.91E-4 ID:SGTC_1599|Compound:8-chlro-1-tetrahydronorharmanone|FD-Score:4.89|P-value:2.42E-6 ID:SGTC_1628|Compound:st004252|FD-Score:3.43|P-value:6.25E-4 ID:SGTC_1636|Compound:st007707|FD-Score:3.64|P-value:3.16E-4 ID:SGTC_1745|Compound:st081495|FD-Score:6.04|P-value:8.42E-9 ID:SGTC_1754|Compound:st042065|FD-Score:8.59|P-value:6.14E-16 ID:SGTC_1759|Compound:st043059|FD-Score:-4.05|P-value:1.08E-4 ID:SGTC_1788|Compound:fluconazole|FD-Score:4.64|P-value:6.85E-6 ID:SGTC_1800|Compound:st050079|FD-Score:4.79|P-value:3.71E-6 ID:SGTC_1802|Compound:st050112|FD-Score:3.57|P-value:3.91E-4 ID:SGTC_1803|Compound:st050068|FD-Score:7.72|P-value:3.01E-13 ID:SGTC_1814|Compound:st052110|FD-Score:-4.14|P-value:7.82E-5 ID:SGTC_1818|Compound:st052136|FD-Score:6.24|P-value:2.88E-9 ID:SGTC_1844|Compound:rapamycin|FD-Score:4.35|P-value:2.25E-5 ID:SGTC_1876|Compound:myriocin|FD-Score:6.71|P-value:1.96E-10 ID:SGTC_1970|Compound:st070122|FD-Score:-4.11|P-value:8.67E-5 ID:SGTC_2021|Compound:4019055|FD-Score:3.39|P-value:7.26E-4 ID:SGTC_2057|Compound:5236571|FD-Score:3.48|P-value:5.37E-4 ID:SGTC_2089|Compound:5281248|FD-Score:3.44|P-value:6.08E-4 ID:SGTC_2248|Compound:tofa|FD-Score:11.00|P-value:5.72E-25 ID:SGTC_2264|Compound:7948757|FD-Score:3.70|P-value:2.53E-4 ID:SGTC_2283|Compound:7944793|FD-Score:3.93|P-value:1.13E-4 ID:SGTC_2309|Compound:7664088|FD-Score:3.32|P-value:8.89E-4 ID:SGTC_2331|Compound:7980721|FD-Score:3.66|P-value:2.92E-4 ID:SGTC_2397|Compound:5161689|FD-Score:3.61|P-value:3.50E-4 ID:SGTC_2416|Compound:tofa|FD-Score:17.00|P-value:4.03E-56 ID:SGTC_2495|Compound:cafestol|FD-Score:-3.65|P-value:4.41E-4 ID:SGTC_2591|Compound:skatole|FD-Score:3.42|P-value:6.60E-4 ID:SGTC_2623|Compound:humulene|FD-Score:-3.72|P-value:3.47E-4 ID:SGTC_2645|Compound:abamectin|FD-Score:4.07|P-value:6.83E-5 ID:SGTC_2659|Compound:sanguinarine|FD-Score:3.41|P-value:6.62E-4 ID:SGTC_2665|Compound:lovastatin|FD-Score:3.60|P-value:3.65E-4 ID:SGTC_2679|Compound:rapamycin|FD-Score:5.84|P-value:2.48E-8 ID:SGTC_2688|Compound:kanamycin b|FD-Score:6.65|P-value:2.71E-10 ID:SGTC_2765|Compound:myclobutanil|FD-Score:5.74|P-value:4.16E-8 ID:SGTC_2778|Compound:tioconazole|FD-Score:4.35|P-value:2.27E-5 ID:SGTC_2816|Compound:7982706|FD-Score:5.09|P-value:9.56E-7 ID:SGTC_2823|Compound:7987624|FD-Score:-3.97|P-value:1.47E-4 ID:SGTC_2830|Compound:7992007|FD-Score:3.35|P-value:8.25E-4 ID:SGTC_2970|Compound:9090938|FD-Score:3.34|P-value:8.41E-4 ID:SGTC_3043|Compound:9092303|FD-Score:-3.41|P-value:9.44E-4 ID:SGTC_3152|Compound:9099253|FD-Score:3.92|P-value:1.17E-4 ID:SGTC_3196|Compound:9111891|FD-Score:-4.13|P-value:8.11E-5 ID:SGTC_3197|Compound:9113331|FD-Score:-5.13|P-value:1.31E-6 ID:SGTC_3306|Compound:9127676|FD-Score:-3.62|P-value:4.79E-4 ID:SGTC_3308|Compound:9128332|FD-Score:3.41|P-value:6.68E-4 ID:SGTC_3339|Compound:9145883|FD-Score:4.01|P-value:8.49E-5 ID:SGTC_3354|Compound:9151612|FD-Score:3.82|P-value:1.67E-4

Top fitness defect scores for YER177W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2416 tofa 17.00 880.0 nM 4.03E-56 sphingolipid biosynthesis & PDR1 6 9.41E-65
2 SGTC_2248 tofa 11.00 1.1 µM 5.72E-25 sphingolipid biosynthesis & PDR1 7 1.47E-28
3 SGTC_1754 st042065 8.59 16.1 µM 6.14E-16 NEO1-PIK1 48 4.31E-18
4 SGTC_1067 clotrimazole 8.28 1.4 µM 5.89E-15 azole & statin 2 5.91E-17
5 SGTC_1803 st050068 7.72 52.4 µM 3.01E-13 NEO1-PIK1 43 5.60E-15
6 SGTC_227 fluconazole 7.45 33.5 µM 1.84E-12 azole & statin 25 4.55E-14
7 SGTC_65 0798-0905 7.28 35.0 µM 5.78E-12 calcium & mitochondrial duress 18 1.71E-13
8 SGTC_221 trifluoperazine 7.22 10.3 µM 8.51E-12 NEO1-PIK1 99 2.67E-13
9 SGTC_1876 myriocin 6.71 605.0 nM 1.96E-10 11 9.97E-12
10 SGTC_2688 kanamycin b 6.65 96.7 µM 2.71E-10 calcium & mitochondrial duress 121 1.45E-11
11 SGTC_902 1120-0002 6.30 2.1 µM 2.05E-9 azole & statin 18 1.50E-10
12 SGTC_1818 st052136 6.24 56.3 µM 2.88E-9 NEO1-PIK1 10 2.22E-10
13 SGTC_1745 st081495 6.04 74.0 µM 8.42E-9 RNA processing & uracil transport 26 7.65E-10
14 SGTC_526 4469-0568 5.96 170.0 µM 1.31E-8 azole & statin 17 1.28E-9
15 SGTC_2679 rapamycin 5.84 1.0 nM 2.48E-8 81 2.66E-9
16 SGTC_1084 voriconazole 5.75 435.0 nM 3.93E-8 azole & statin 8 4.52E-9
17 SGTC_2765 myclobutanil 5.74 7.7 µM 4.16E-8 azole & statin 14 4.83E-9
18 SGTC_747 3448-8087 5.25 38.7 µM 4.62E-7 8 7.70E-8
19 SGTC_2816 7982706 5.09 58.4 µM 9.56E-7 azole & statin 28 1.78E-7
20 SGTC_1599 8-chlro-1-tetrahydronorharmanone 4.89 90.6 µM 2.42E-6 16 5.16E-7

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.316 8.34E-79 YKR028W SAP190 hom Protein that forms a complex with the Sit4p protein phosphatase; required for Sit4p function; member of a family of similar proteins including Sap4p, Sap155p, and Sap185p; SAP190 has a paralog, SAP185, that arose from the whole genome duplication
0.239 7.26E-45 YHR012W VPS29 hom Endosomal protein that is a subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps35p and Vps26p that selects cargo proteins for endosome-to-Golgi retrieval
0.223 6.54E-39 YLR420W URA4 hom Dihydroorotase, catalyzes the third enzymatic step in the de novo biosynthesis of pyrimidines, converting carbamoyl-L-aspartate into dihydroorotate
0.219 8.65E-38 YJL054W TIM54 het Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane
0.212 1.71E-35 YGL045W RIM8 hom Protein involved in proteolytic activation of Rim101p in response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family
0.207 7.19E-34 YGR122W YGR122W hom Protein that may be involved in pH regulation; probable ortholog of A. nidulans PalC, which is involved in pH regulation and binds to the ESCRT-III complex; null mutant does not properly process Rim101p and has decreased resistance to rapamycin; GFP-fusion protein is cytoplasmic; relative distribution to cytoplasm increases upon DNA replication stress
0.206 2.05E-33 YOR357C SNX3 hom Sorting nexin required to maintain late-Golgi resident enzymes in their proper location by recycling molecules from the prevacuolar compartment; contains a PX domain and sequence similarity to human Snx3p
0.204 8.97E-33 YMR154C RIM13 hom Calpain-like cysteine protease; involved in proteolytic activation of Rim101p in response to alkaline pH; localizes to punctate structures in alkaline conditions and in vps4 mutant; has similarity to A. nidulans palB
0.203 1.06E-32 YOR275C RIM20 hom Protein involved in proteolytic activation of Rim101p in response to alkaline pH; PalA/AIP1/Alix family member; interaction with the ESCRT-III subunit Snf7p suggests a relationship between pH response and multivesicular body formation
0.203 1.30E-32 YGR166W TRS65 hom Subunit of TRAPPII, a multimeric guanine nucleotide-exchange factor for Ypt1p; involved in intra-Golgi traffic and the retrograde pathway from the endosome to Golgi; role in cell wall beta-glucan biosynthesis and the stress response
0.197 7.66E-31 YNL294C RIM21 hom pH sensor molecule, component of the RIM101 pathway; has a role in cell wall construction and alkaline pH response; is glycosylated and phosphorylated; interacts with Dfg16p and Rim9p to form a pH-sensing complex; localization to the plasma membrane is dependent on Dfg16p and Rim9p; has similarity to A. nidulans PalH
0.196 2.81E-30 YBR246W RRT2 hom Protein required for last step of diphthamide biosynthesis; deletion leads to resistance to sordarin and accumulation of diphthine; WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rtt10p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription
0.195 5.51E-30 YPL202C AFT2 hom Iron-regulated transcriptional activator; activates genes involved in intracellular iron use and required for iron homeostasis and resistance to oxidative stress; AFT2 has a paralog, AFT1, that arose from the whole genome duplication
0.194 9.20E-30 YGR202C PCT1 hom Cholinephosphate cytidylyltransferase; a rate-determining enzyme of the CDP-choline pathway for phosphatidylcholine synthesis, inhibited by Sec14p, activated upon lipid-binding; contains an element within the regulatory domain involved in both silencing and activation of enzymatic activity
0.193 1.74E-29 YHR045W_p YHR045W_p hom Putative protein of unknown function; possible role in iron metabolism and/or amino acid and carbohydrate metabolism; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum