YER185W / PUG1

Plasma membrane protein with roles in the uptake of protoprophyrin IX and the efflux of heme; expression is induced under both low-heme and low-oxygen conditions; member of the fungal lipid-translocating exporter (LTE) family of proteins

Zygosity: Homozygous strain
fixedexpanded
Profile for YER185W / PUG1

Click on Significant Values for Screen Details ID:SGTC_23|Compound:0213-0011|FD-Score:4.09|P-value:2.73E-5 ID:SGTC_69|Compound:0096-0274|FD-Score:5.07|P-value:2.92E-7 ID:SGTC_119|Compound:0121-0043|FD-Score:-3.75|P-value:1.60E-4 ID:SGTC_164|Compound:4100-3994|FD-Score:3.33|P-value:4.80E-4 ID:SGTC_167|Compound:3672-0051|FD-Score:4.28|P-value:1.19E-5 ID:SGTC_183|Compound:3914-0051|FD-Score:3.29|P-value:5.69E-4 ID:SGTC_230|Compound:MPP+|FD-Score:-3.69|P-value:1.95E-4 ID:SGTC_231|Compound:b-estradiol|FD-Score:-3.26|P-value:8.84E-4 ID:SGTC_246|Compound:cycloheximide|FD-Score:3.63|P-value:1.66E-4 ID:SGTC_247|Compound:mechlorethamine|FD-Score:4.06|P-value:3.04E-5 ID:SGTC_249|Compound:wiskostatin|FD-Score:3.58|P-value:2.01E-4 ID:SGTC_382|Compound:3229-0966|FD-Score:3.22|P-value:7.16E-4 ID:SGTC_431|Compound:k089-0097|FD-Score:3.53|P-value:2.39E-4 ID:SGTC_434|Compound:1-hexadecyl-2-methylglycero-3 PC|FD-Score:-4.19|P-value:2.73E-5 ID:SGTC_565|Compound:1418-0001|FD-Score:4.00|P-value:3.97E-5 ID:SGTC_570|Compound:0987-0065|FD-Score:3.67|P-value:1.42E-4 ID:SGTC_572|Compound:0960-0087|FD-Score:5.16|P-value:1.86E-7 ID:SGTC_738|Compound:1315-0003|FD-Score:3.52|P-value:2.49E-4 ID:SGTC_872|Compound:0588-0139|FD-Score:5.71|P-value:9.55E-9 ID:SGTC_967|Compound:1306-0166|FD-Score:3.78|P-value:9.49E-5 ID:SGTC_1097|Compound:itavastatin|FD-Score:-3.46|P-value:4.56E-4 ID:SGTC_1124|Compound:fisetin|FD-Score:-3.95|P-value:7.26E-5 ID:SGTC_1156|Compound:4092-0367|FD-Score:4.85|P-value:8.67E-7 ID:SGTC_1265|Compound:0819-1001|FD-Score:3.66|P-value:1.47E-4 ID:SGTC_1362|Compound:1611-0397|FD-Score:4.15|P-value:2.09E-5 ID:SGTC_1390|Compound:3413-2614|FD-Score:3.22|P-value:7.16E-4 ID:SGTC_1409|Compound:3909-8004|FD-Score:3.47|P-value:3.02E-4 ID:SGTC_1426|Compound:4048-4480|FD-Score:3.86|P-value:6.79E-5 ID:SGTC_1455|Compound:4262-0120|FD-Score:3.17|P-value:8.51E-4 ID:SGTC_1639|Compound:Lupinine o-methylbenzoicacid ester hydrochloride|FD-Score:3.58|P-value:2.02E-4 ID:SGTC_1659|Compound:st012954|FD-Score:3.72|P-value:1.18E-4 ID:SGTC_1666|Compound:st014829|FD-Score:-3.40|P-value:5.48E-4 ID:SGTC_1766|Compound:st045414|FD-Score:3.30|P-value:5.40E-4 ID:SGTC_1774|Compound:st048371|FD-Score:3.98|P-value:4.21E-5 ID:SGTC_1792|Compound:st045773|FD-Score:5.12|P-value:2.33E-7 ID:SGTC_1825|Compound:st052143|FD-Score:6.24|P-value:4.17E-10 ID:SGTC_1830|Compound:st055982|FD-Score:-4.36|P-value:1.35E-5 ID:SGTC_1843|Compound:st055809|FD-Score:-5.23|P-value:2.25E-7 ID:SGTC_1854|Compound:st056233|FD-Score:-3.62|P-value:2.51E-4 ID:SGTC_1856|Compound:st057054|FD-Score:-3.26|P-value:8.83E-4 ID:SGTC_1861|Compound:st057647|FD-Score:3.14|P-value:9.33E-4 ID:SGTC_2156|Compound:5649887|FD-Score:3.86|P-value:6.93E-5 ID:SGTC_2203|Compound:7227154|FD-Score:3.15|P-value:9.03E-4 ID:SGTC_2369|Compound:9071472|FD-Score:3.32|P-value:5.04E-4 ID:SGTC_2572|Compound:astragaloside iv|FD-Score:3.45|P-value:3.18E-4 ID:SGTC_2676|Compound:harmalol|FD-Score:5.40|P-value:5.34E-8 ID:SGTC_2769|Compound:toremifene|FD-Score:3.55|P-value:2.25E-4 ID:SGTC_2851|Compound:9015922|FD-Score:3.13|P-value:9.58E-4 ID:SGTC_2874|Compound:9043904|FD-Score:3.55|P-value:2.20E-4 ID:SGTC_2980|Compound:9014342|FD-Score:-4.53|P-value:6.40E-6 ID:SGTC_2987|Compound:9018338|FD-Score:4.10|P-value:2.56E-5 ID:SGTC_3103|Compound:9120822|FD-Score:-4.64|P-value:3.83E-6 ID:SGTC_3108|Compound:9121785|FD-Score:3.24|P-value:6.72E-4 ID:SGTC_3120|Compound:9124077|FD-Score:-3.46|P-value:4.54E-4 ID:SGTC_3137|Compound:9097352|FD-Score:4.54|P-value:3.72E-6 ID:SGTC_3139|Compound:9095020|FD-Score:-3.92|P-value:8.09E-5 ID:SGTC_3150|Compound:9097574|FD-Score:3.90|P-value:5.80E-5 ID:SGTC_3152|Compound:9099253|FD-Score:4.18|P-value:1.83E-5 ID:SGTC_3198|Compound:9112019|FD-Score:3.28|P-value:5.88E-4 ID:SGTC_3199|Compound:9111318|FD-Score:4.07|P-value:2.97E-5 ID:SGTC_3311|Compound:9131146|FD-Score:-3.51|P-value:3.79E-4 ID:SGTC_23|Compound:0213-0011|FD-Score:4.09|P-value:2.73E-5 ID:SGTC_69|Compound:0096-0274|FD-Score:5.07|P-value:2.92E-7 ID:SGTC_119|Compound:0121-0043|FD-Score:-3.75|P-value:1.60E-4 ID:SGTC_164|Compound:4100-3994|FD-Score:3.33|P-value:4.80E-4 ID:SGTC_167|Compound:3672-0051|FD-Score:4.28|P-value:1.19E-5 ID:SGTC_183|Compound:3914-0051|FD-Score:3.29|P-value:5.69E-4 ID:SGTC_230|Compound:MPP+|FD-Score:-3.69|P-value:1.95E-4 ID:SGTC_231|Compound:b-estradiol|FD-Score:-3.26|P-value:8.84E-4 ID:SGTC_246|Compound:cycloheximide|FD-Score:3.63|P-value:1.66E-4 ID:SGTC_247|Compound:mechlorethamine|FD-Score:4.06|P-value:3.04E-5 ID:SGTC_249|Compound:wiskostatin|FD-Score:3.58|P-value:2.01E-4 ID:SGTC_382|Compound:3229-0966|FD-Score:3.22|P-value:7.16E-4 ID:SGTC_431|Compound:k089-0097|FD-Score:3.53|P-value:2.39E-4 ID:SGTC_434|Compound:1-hexadecyl-2-methylglycero-3 PC|FD-Score:-4.19|P-value:2.73E-5 ID:SGTC_565|Compound:1418-0001|FD-Score:4.00|P-value:3.97E-5 ID:SGTC_570|Compound:0987-0065|FD-Score:3.67|P-value:1.42E-4 ID:SGTC_572|Compound:0960-0087|FD-Score:5.16|P-value:1.86E-7 ID:SGTC_738|Compound:1315-0003|FD-Score:3.52|P-value:2.49E-4 ID:SGTC_872|Compound:0588-0139|FD-Score:5.71|P-value:9.55E-9 ID:SGTC_967|Compound:1306-0166|FD-Score:3.78|P-value:9.49E-5 ID:SGTC_1097|Compound:itavastatin|FD-Score:-3.46|P-value:4.56E-4 ID:SGTC_1124|Compound:fisetin|FD-Score:-3.95|P-value:7.26E-5 ID:SGTC_1156|Compound:4092-0367|FD-Score:4.85|P-value:8.67E-7 ID:SGTC_1265|Compound:0819-1001|FD-Score:3.66|P-value:1.47E-4 ID:SGTC_1362|Compound:1611-0397|FD-Score:4.15|P-value:2.09E-5 ID:SGTC_1390|Compound:3413-2614|FD-Score:3.22|P-value:7.16E-4 ID:SGTC_1409|Compound:3909-8004|FD-Score:3.47|P-value:3.02E-4 ID:SGTC_1426|Compound:4048-4480|FD-Score:3.86|P-value:6.79E-5 ID:SGTC_1455|Compound:4262-0120|FD-Score:3.17|P-value:8.51E-4 ID:SGTC_1639|Compound:Lupinine o-methylbenzoicacid ester hydrochloride|FD-Score:3.58|P-value:2.02E-4 ID:SGTC_1659|Compound:st012954|FD-Score:3.72|P-value:1.18E-4 ID:SGTC_1666|Compound:st014829|FD-Score:-3.40|P-value:5.48E-4 ID:SGTC_1766|Compound:st045414|FD-Score:3.30|P-value:5.40E-4 ID:SGTC_1774|Compound:st048371|FD-Score:3.98|P-value:4.21E-5 ID:SGTC_1792|Compound:st045773|FD-Score:5.12|P-value:2.33E-7 ID:SGTC_1825|Compound:st052143|FD-Score:6.24|P-value:4.17E-10 ID:SGTC_1830|Compound:st055982|FD-Score:-4.36|P-value:1.35E-5 ID:SGTC_1843|Compound:st055809|FD-Score:-5.23|P-value:2.25E-7 ID:SGTC_1854|Compound:st056233|FD-Score:-3.62|P-value:2.51E-4 ID:SGTC_1856|Compound:st057054|FD-Score:-3.26|P-value:8.83E-4 ID:SGTC_1861|Compound:st057647|FD-Score:3.14|P-value:9.33E-4 ID:SGTC_2156|Compound:5649887|FD-Score:3.86|P-value:6.93E-5 ID:SGTC_2203|Compound:7227154|FD-Score:3.15|P-value:9.03E-4 ID:SGTC_2369|Compound:9071472|FD-Score:3.32|P-value:5.04E-4 ID:SGTC_2572|Compound:astragaloside iv|FD-Score:3.45|P-value:3.18E-4 ID:SGTC_2676|Compound:harmalol|FD-Score:5.40|P-value:5.34E-8 ID:SGTC_2769|Compound:toremifene|FD-Score:3.55|P-value:2.25E-4 ID:SGTC_2851|Compound:9015922|FD-Score:3.13|P-value:9.58E-4 ID:SGTC_2874|Compound:9043904|FD-Score:3.55|P-value:2.20E-4 ID:SGTC_2980|Compound:9014342|FD-Score:-4.53|P-value:6.40E-6 ID:SGTC_2987|Compound:9018338|FD-Score:4.10|P-value:2.56E-5 ID:SGTC_3103|Compound:9120822|FD-Score:-4.64|P-value:3.83E-6 ID:SGTC_3108|Compound:9121785|FD-Score:3.24|P-value:6.72E-4 ID:SGTC_3120|Compound:9124077|FD-Score:-3.46|P-value:4.54E-4 ID:SGTC_3137|Compound:9097352|FD-Score:4.54|P-value:3.72E-6 ID:SGTC_3139|Compound:9095020|FD-Score:-3.92|P-value:8.09E-5 ID:SGTC_3150|Compound:9097574|FD-Score:3.90|P-value:5.80E-5 ID:SGTC_3152|Compound:9099253|FD-Score:4.18|P-value:1.83E-5 ID:SGTC_3198|Compound:9112019|FD-Score:3.28|P-value:5.88E-4 ID:SGTC_3199|Compound:9111318|FD-Score:4.07|P-value:2.97E-5 ID:SGTC_3311|Compound:9131146|FD-Score:-3.51|P-value:3.79E-4

Top fitness defect scores for YER185W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1825 st052143 6.24 62.6 µM 4.17E-10 2 2.13E-10
2 SGTC_872 0588-0139 5.71 234.0 µM 9.55E-9 9 5.56E-9
3 SGTC_2676 harmalol 5.40 100.0 µM 5.34E-8 mitochondrial processes 2 3.34E-8
4 SGTC_572 0960-0087 5.16 17.7 µM 1.86E-7 6 1.23E-7
5 SGTC_1792 st045773 5.12 43.6 µM 2.33E-7 10 1.55E-7
6 SGTC_69 0096-0274 5.07 206.7 µM 2.92E-7 mitochondrial processes 17 1.96E-7
7 SGTC_1156 4092-0367 4.85 4.9 µM 8.67E-7 6 6.07E-7
8 SGTC_3137 9097352 4.54 49.5 µM 3.72E-6 24 2.76E-6
9 SGTC_167 3672-0051 4.28 66.2 µM 1.19E-5 35 9.26E-6
10 SGTC_3152 9099253 4.18 49.5 µM 1.83E-5 NEO1-PIK1 117 1.44E-5
11 SGTC_1362 1611-0397 4.15 101.0 µM 2.09E-5 17 1.66E-5
12 SGTC_2987 9018338 4.10 71.4 µM 2.56E-5 RPP1 & pyrimidine depletion 12 2.04E-5
13 SGTC_23 0213-0011 4.09 40.0 µM 2.73E-5 copper-dependent oxidative stress 22 2.19E-5
14 SGTC_3199 9111318 4.07 49.5 µM 2.97E-5 16 2.39E-5
15 SGTC_247 mechlorethamine 4.06 29.7 µM 3.04E-5 DNA damage response 13 2.45E-5
16 SGTC_565 1418-0001 4.00 44.5 µM 3.97E-5 17 3.23E-5
17 SGTC_1774 st048371 3.98 48.5 µM 4.21E-5 RNA processing & uracil transport 16 3.43E-5
18 SGTC_3150 9097574 3.90 49.5 µM 5.80E-5 10 4.78E-5
19 SGTC_1426 4048-4480 3.86 71.7 µM 6.79E-5 60S ribosome export 19 5.63E-5
20 SGTC_2156 5649887 3.86 115.0 µM 6.93E-5 RPP1 & pyrimidine depletion 12 5.75E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.099 9.29E-9 YKL052C ASK1 het Essential subunit of the Dam1 complex (aka DASH complex); couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; phosphorylated during the cell cycle by cyclin-dependent kinases; protein abundance increases in response to DNA replication stress
0.078 6.53E-6 YER025W GCD11 het Gamma subunit of the translation initiation factor eIF2, involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met
0.075 1.47E-5 YLR109W AHP1 hom Thiol-specific peroxiredoxin, reduces hydroperoxides to protect against oxidative damage; function in vivo requires covalent conjugation to Urm1p
0.073 2.15E-5 YAR002C-A ERP1 hom Member of the p24 family involved in ER to Golgi transport; forms heterotrimeric complex with Erp2p, Emp24p, and Erv25p; localized to COPII-coated vesicles; ERP1 has a paralog, ERP6, that arose from the whole genome duplication
0.073 2.43E-5 YNL014W HEF3 hom Translational elongation factor EF-3; member of the ABC superfamily; stimulates EF-1 alpha-dependent binding of aminoacyl-tRNA by the ribosome; normally expressed in zinc deficient cells; HEF3 has a paralog, YEF3, that arose from the whole genome duplication
0.069 6.10E-5 YMR003W AIM34 hom Protein of unknown function; GFP-fusion protein localizes to the mitochondria; null mutant is viable and displays reduced frequency of mitochondrial genome loss
0.067 9.33E-5 YNL236W SIN4 hom Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; contributes to both postive and negative transcriptional regulation; dispensible for basal transcription
0.066 1.36E-4 YJR126C VPS70 hom Protein of unknown function involved in vacuolar protein sorting
0.064 2.21E-4 YBR182C SMP1 hom Putative transcription factor of the MADS-box family; involved in regulating the response to osmotic stress; SMP1 has a paralog, RLM1, that arose from the whole genome duplication
0.064 2.33E-4 YMR053C STB2 hom Protein that interacts with Sin3p in a two-hybrid assay and is part of a large protein complex with Sin3p and Stb1p
0.063 2.77E-4 YDR154C_d YDR154C_d hom Dubious open reading frame, null mutant exhibits synthetic phenotype with alpha-synuclein
0.062 3.09E-4 YGL232W TAN1 hom Putative tRNA acetyltransferase; RNA-binding protein required for the formation of the modified nucleoside N(4)-acetylcytidine in serine and leucine tRNAs but not required for the same modification in 18S rRNA; protein abundance increases in response to DNA replication stress
0.062 3.14E-4 YGL044C RNA15 het Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; interacts with the A-rich polyadenylation signal in complex with Rna14p and Hrp1p; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping
0.062 3.46E-4 YMR009W ADI1 hom Acireductone dioxygenease involved in the methionine salvage pathway; ortholog of human MTCBP-1; transcribed as polycistronic mRNA with YMR010W and regulated post-transcriptionally by RNase III (Rnt1p) cleavage; ADI1 mRNA is induced in heat shock conditions
0.060 4.90E-4 YOR242C SSP2 hom Sporulation specific protein that localizes to the spore wall; required for sporulation at a point after meiosis II and during spore wall formation; SSP2 expression is induced midway in meiosis