YFL001W / DEG1

tRNA:pseudouridine synthase, introduces pseudouridines at position 38 or 39 in tRNA, important for maintenance of translation efficiency and normal cell growth, localizes to both the nucleus and cytoplasm; non-essential for viability

Zygosity: Homozygous strain
fixedexpanded
Profile for YFL001W / DEG1

Click on Significant Values for Screen Details ID:SGTC_2|Compound:0422-0095|FD-Score:-3.65|P-value:5.36E-4 ID:SGTC_131|Compound:0141-0277|FD-Score:3.68|P-value:5.33E-4 ID:SGTC_193|Compound:1273-0060|FD-Score:-4.27|P-value:6.60E-5 ID:SGTC_299|Compound:1273-0059|FD-Score:-9.49|P-value:1.07E-17 ID:SGTC_310|Compound:1273-0058|FD-Score:-13.50|P-value:7.27E-34 ID:SGTC_335|Compound:4077-0256|FD-Score:-4.43|P-value:3.56E-5 ID:SGTC_679|Compound:0422-0091|FD-Score:3.58|P-value:7.25E-4 ID:SGTC_700|Compound:3453-2283|FD-Score:4.56|P-value:2.49E-5 ID:SGTC_1260|Compound:0700-1617|FD-Score:5.59|P-value:3.23E-7 ID:SGTC_1370|Compound:2001-0092|FD-Score:7.86|P-value:1.21E-12 ID:SGTC_1459|Compound:k035-0031|FD-Score:4.15|P-value:1.12E-4 ID:SGTC_1732|Compound:st034308|FD-Score:4.21|P-value:9.01E-5 ID:SGTC_1757|Compound:st041620|FD-Score:-3.67|P-value:5.03E-4 ID:SGTC_1759|Compound:st043059|FD-Score:4.16|P-value:1.06E-4 ID:SGTC_1766|Compound:st045414|FD-Score:3.50|P-value:9.13E-4 ID:SGTC_1829|Compound:berberine|FD-Score:-3.72|P-value:4.34E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:10.90|P-value:8.26E-23 ID:SGTC_1870|Compound:st057514|FD-Score:4.59|P-value:2.16E-5 ID:SGTC_1872|Compound:amphotericin b|FD-Score:3.60|P-value:6.83E-4 ID:SGTC_1876|Compound:myriocin|FD-Score:-3.56|P-value:6.99E-4 ID:SGTC_1935|Compound:st074532|FD-Score:3.60|P-value:6.74E-4 ID:SGTC_1937|Compound:st074714|FD-Score:-4.14|P-value:1.05E-4 ID:SGTC_2047|Compound:4141938|FD-Score:4.10|P-value:1.32E-4 ID:SGTC_2272|Compound:7805186|FD-Score:-4.24|P-value:7.29E-5 ID:SGTC_2305|Compound:7443522|FD-Score:-4.46|P-value:3.26E-5 ID:SGTC_2337|Compound:9009472|FD-Score:-3.53|P-value:7.67E-4 ID:SGTC_2536|Compound:dehydroabietamide|FD-Score:4.03|P-value:1.66E-4 ID:SGTC_2549|Compound:cedrol|FD-Score:-4.32|P-value:5.44E-5 ID:SGTC_2582|Compound:agaric acid|FD-Score:5.81|P-value:1.13E-7 ID:SGTC_2604|Compound:4'-hydroxychalcone|FD-Score:4.29|P-value:6.60E-5 ID:SGTC_2608|Compound:thymoquinone|FD-Score:-3.50|P-value:8.58E-4 ID:SGTC_2611|Compound:pyromycin|FD-Score:5.25|P-value:1.44E-6 ID:SGTC_2634|Compound:4'-methoxyflavone|FD-Score:6.12|P-value:2.50E-8 ID:SGTC_2679|Compound:rapamycin|FD-Score:5.39|P-value:7.97E-7 ID:SGTC_2706|Compound:st078239|FD-Score:4.25|P-value:7.70E-5 ID:SGTC_2752|Compound:diclazuril|FD-Score:-4.81|P-value:8.11E-6 ID:SGTC_2852|Compound:9023854|FD-Score:5.69|P-value:2.02E-7 ID:SGTC_2855|Compound:9016525|FD-Score:3.54|P-value:8.22E-4 ID:SGTC_2882|Compound:9058044|FD-Score:4.03|P-value:1.66E-4 ID:SGTC_2942|Compound:9052777|FD-Score:3.75|P-value:4.20E-4 ID:SGTC_2943|Compound:9068529|FD-Score:4.13|P-value:1.18E-4 ID:SGTC_2948|Compound:9070189|FD-Score:-3.82|P-value:3.11E-4 ID:SGTC_2991|Compound:9074669|FD-Score:-3.45|P-value:9.90E-4 ID:SGTC_3018|Compound:9082602|FD-Score:-5.50|P-value:4.18E-7 ID:SGTC_3113|Compound:9123498|FD-Score:4.32|P-value:6.10E-5 ID:SGTC_3158|Compound:9100007|FD-Score:5.76|P-value:1.45E-7 ID:SGTC_3296|Compound:9122258|FD-Score:-3.48|P-value:8.94E-4 ID:SGTC_2|Compound:0422-0095|FD-Score:-3.65|P-value:5.36E-4 ID:SGTC_131|Compound:0141-0277|FD-Score:3.68|P-value:5.33E-4 ID:SGTC_193|Compound:1273-0060|FD-Score:-4.27|P-value:6.60E-5 ID:SGTC_299|Compound:1273-0059|FD-Score:-9.49|P-value:1.07E-17 ID:SGTC_310|Compound:1273-0058|FD-Score:-13.50|P-value:7.27E-34 ID:SGTC_335|Compound:4077-0256|FD-Score:-4.43|P-value:3.56E-5 ID:SGTC_679|Compound:0422-0091|FD-Score:3.58|P-value:7.25E-4 ID:SGTC_700|Compound:3453-2283|FD-Score:4.56|P-value:2.49E-5 ID:SGTC_1260|Compound:0700-1617|FD-Score:5.59|P-value:3.23E-7 ID:SGTC_1370|Compound:2001-0092|FD-Score:7.86|P-value:1.21E-12 ID:SGTC_1459|Compound:k035-0031|FD-Score:4.15|P-value:1.12E-4 ID:SGTC_1732|Compound:st034308|FD-Score:4.21|P-value:9.01E-5 ID:SGTC_1757|Compound:st041620|FD-Score:-3.67|P-value:5.03E-4 ID:SGTC_1759|Compound:st043059|FD-Score:4.16|P-value:1.06E-4 ID:SGTC_1766|Compound:st045414|FD-Score:3.50|P-value:9.13E-4 ID:SGTC_1829|Compound:berberine|FD-Score:-3.72|P-value:4.34E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:10.90|P-value:8.26E-23 ID:SGTC_1870|Compound:st057514|FD-Score:4.59|P-value:2.16E-5 ID:SGTC_1872|Compound:amphotericin b|FD-Score:3.60|P-value:6.83E-4 ID:SGTC_1876|Compound:myriocin|FD-Score:-3.56|P-value:6.99E-4 ID:SGTC_1935|Compound:st074532|FD-Score:3.60|P-value:6.74E-4 ID:SGTC_1937|Compound:st074714|FD-Score:-4.14|P-value:1.05E-4 ID:SGTC_2047|Compound:4141938|FD-Score:4.10|P-value:1.32E-4 ID:SGTC_2272|Compound:7805186|FD-Score:-4.24|P-value:7.29E-5 ID:SGTC_2305|Compound:7443522|FD-Score:-4.46|P-value:3.26E-5 ID:SGTC_2337|Compound:9009472|FD-Score:-3.53|P-value:7.67E-4 ID:SGTC_2536|Compound:dehydroabietamide|FD-Score:4.03|P-value:1.66E-4 ID:SGTC_2549|Compound:cedrol|FD-Score:-4.32|P-value:5.44E-5 ID:SGTC_2582|Compound:agaric acid|FD-Score:5.81|P-value:1.13E-7 ID:SGTC_2604|Compound:4'-hydroxychalcone|FD-Score:4.29|P-value:6.60E-5 ID:SGTC_2608|Compound:thymoquinone|FD-Score:-3.50|P-value:8.58E-4 ID:SGTC_2611|Compound:pyromycin|FD-Score:5.25|P-value:1.44E-6 ID:SGTC_2634|Compound:4'-methoxyflavone|FD-Score:6.12|P-value:2.50E-8 ID:SGTC_2679|Compound:rapamycin|FD-Score:5.39|P-value:7.97E-7 ID:SGTC_2706|Compound:st078239|FD-Score:4.25|P-value:7.70E-5 ID:SGTC_2752|Compound:diclazuril|FD-Score:-4.81|P-value:8.11E-6 ID:SGTC_2852|Compound:9023854|FD-Score:5.69|P-value:2.02E-7 ID:SGTC_2855|Compound:9016525|FD-Score:3.54|P-value:8.22E-4 ID:SGTC_2882|Compound:9058044|FD-Score:4.03|P-value:1.66E-4 ID:SGTC_2942|Compound:9052777|FD-Score:3.75|P-value:4.20E-4 ID:SGTC_2943|Compound:9068529|FD-Score:4.13|P-value:1.18E-4 ID:SGTC_2948|Compound:9070189|FD-Score:-3.82|P-value:3.11E-4 ID:SGTC_2991|Compound:9074669|FD-Score:-3.45|P-value:9.90E-4 ID:SGTC_3018|Compound:9082602|FD-Score:-5.50|P-value:4.18E-7 ID:SGTC_3113|Compound:9123498|FD-Score:4.32|P-value:6.10E-5 ID:SGTC_3158|Compound:9100007|FD-Score:5.76|P-value:1.45E-7 ID:SGTC_3296|Compound:9122258|FD-Score:-3.48|P-value:8.94E-4

Top fitness defect scores for YFL001W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1844 rapamycin 10.90 4.0 nM 8.26E-23 33 3.50E-28
2 SGTC_1370 2001-0092 7.86 44.6 µM 1.21E-12 1 1.88E-15
3 SGTC_2634 4'-methoxyflavone 6.12 100.0 µM 2.50E-8 7 4.71E-10
4 SGTC_2582 agaric acid 5.81 100.0 µM 1.13E-7 redox potentiating 167 3.10E-9
5 SGTC_3158 9100007 5.76 49.5 µM 1.45E-7 5 4.25E-9
6 SGTC_2852 9023854 5.69 52.0 µM 2.02E-7 11 6.40E-9
7 SGTC_1260 0700-1617 5.59 7.8 µM 3.23E-7 calcium & mitochondrial duress 12 1.15E-8
8 SGTC_2679 rapamycin 5.39 1.0 nM 7.97E-7 91 3.56E-8
9 SGTC_2611 pyromycin 5.25 23.9 µM 1.44E-6 5 7.46E-8
10 SGTC_1870 st057514 4.59 26.5 µM 2.16E-5 RSC & ERG11 12 2.17E-6
11 SGTC_700 3453-2283 4.56 142.0 µM 2.49E-5 amide catabolism 13 2.60E-6
12 SGTC_3113 9123498 4.32 49.5 µM 6.10E-5 9 7.93E-6
13 SGTC_2604 4'-hydroxychalcone 4.29 11.3 µM 6.60E-5 27 8.74E-6
14 SGTC_2706 st078239 4.25 82.5 µM 7.70E-5 5 1.06E-5
15 SGTC_1732 st034308 4.21 51.8 µM 9.01E-5 21 1.29E-5
16 SGTC_1759 st043059 4.16 70.0 µM 1.06E-4 amide catabolism 21 1.58E-5
17 SGTC_1459 k035-0031 4.15 35.7 µM 1.12E-4 DNA intercalators 20 1.68E-5
18 SGTC_2943 9068529 4.13 45.3 µM 1.18E-4 15 1.80E-5
19 SGTC_2047 4141938 4.10 200.0 µM 1.32E-4 8 2.06E-5
20 SGTC_2536 dehydroabietamide 4.03 32.0 µM 1.66E-4 33 2.74E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.243 4.13E-46 YJR066W TOR1 hom PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that controls growth in response to nutrients by regulating translation, transcription, ribosome biogenesis, nutrient transport and autophagy; involved in meiosis; TOR1 has a paralog, TOR2, that arose from the whole genome duplication
0.218 2.03E-37 YKL203C TOR2 het PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that regulates growth in response to nutrients and TORC2, a complex that regulates cell-cycle dependent polarization of the actin cytoskeleton; involved in meiosis; TOR2 has a paralog, TOR1, that arose from the whole genome duplication
0.211 9.58E-35 YLR315W NKP2 hom Central kinetochore protein and subunit of the Ctf19 complex; mutants have elevated rates of chromosome loss; orthologous to fission yeast kinetochore protein cnl2
0.208 5.94E-34 YML121W GTR1 hom Cytoplasmic GTP binding protein and negative regulator of the Ran/Tc4 GTPase cycle; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport and telomeric silencing; similar to human RagA and RagB
0.207 7.35E-34 YOR073W SGO1 hom Component of the spindle checkpoint, involved in sensing lack of tension on mitotic chromosomes; protects centromeric Rec8p at meiosis I; required for accurate chromosomal segregation at meiosis II and for mitotic chromosome stability
0.205 3.30E-33 YGR163W GTR2 hom Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD
0.202 3.29E-32 YDL172C_d YDL172C_d hom Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data
0.201 8.49E-32 YLR114C AVL9 hom Conserved protein involved in exocytic transport from the Golgi; mutation is synthetically lethal with apl2 vps1 double mutation; member of a protein superfamily with orthologs in diverse organisms; relocalizes from bud neck to cytoplasm upon DNA replication stress
0.189 2.93E-28 YKL110C KTI12 hom Protein that plays a role, with Elongator complex, in modification of wobble nucleosides in tRNA; involved in sensitivity to G1 arrest induced by zymocin; interacts with chromatin throughout the genome; also interacts with Cdc19p
0.176 8.05E-25 YDR417C_d YDR417C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RPL12B/YDR418W
0.173 4.93E-24 YAL059W ECM1 hom Pre-ribosomal factor involved in 60S ribosomal protein subunit export; associates with the pre-60S particle; shuttles between the nucleus and cytoplasm
0.168 1.67E-22 YGL211W NCS6 hom Protein required for thiolation of the uridine at the wobble position of Gln, Lys, and Glu tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae
0.167 2.00E-22 YHR187W IKI1 hom Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; iki1 mutations confer resistance to the K. lactis toxin zymocin
0.166 3.40E-22 YHR186C KOG1 het Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors
0.161 5.86E-21 YLR384C IKI3 hom Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; maintains structural integrity of Elongator; homolog of human IKAP, mutations in which cause familial dysautonomia (FD)