YFL003C / MSH4

Protein involved in meiotic recombination, required for normal levels of crossing over, colocalizes with Zip2p to discrete foci on meiotic chromosomes, has homology to bacterial MutS protein

Zygosity: Homozygous strain
fixedexpanded
Profile for YFL003C / MSH4

Click on Significant Values for Screen Details ID:SGTC_163|Compound:1327-0036|FD-Score:2.64|P-value:6.99E-4 ID:SGTC_166|Compound:k072-0058|FD-Score:-4.18|P-value:1.36E-7 ID:SGTC_230|Compound:MPP+|FD-Score:-2.51|P-value:9.74E-4 ID:SGTC_238|Compound:5hr heat shock (37°C) + nsc-638432|FD-Score:-2.58|P-value:7.30E-4 ID:SGTC_374|Compound:0880-0231|FD-Score:3.68|P-value:4.13E-6 ID:SGTC_380|Compound:3970-0968|FD-Score:3.67|P-value:4.26E-6 ID:SGTC_448|Compound:monastrol|FD-Score:3.82|P-value:1.85E-6 ID:SGTC_460|Compound:trequinsin|FD-Score:3.05|P-value:1.11E-4 ID:SGTC_517|Compound:1365-0151|FD-Score:-3.46|P-value:1.03E-5 ID:SGTC_518|Compound:1021-0119|FD-Score:-3.16|P-value:5.03E-5 ID:SGTC_686|Compound:1319-0111|FD-Score:2.75|P-value:4.38E-4 ID:SGTC_1110|Compound:3-hydroxy-3'-methoxyflavone|FD-Score:2.80|P-value:3.51E-4 ID:SGTC_1272|Compound:0839-0021|FD-Score:3.29|P-value:3.36E-5 ID:SGTC_1274|Compound:0865-0114|FD-Score:2.62|P-value:7.60E-4 ID:SGTC_1678|Compound:st019265|FD-Score:3.48|P-value:1.26E-5 ID:SGTC_2013|Compound:4024628|FD-Score:3.84|P-value:1.59E-6 ID:SGTC_2014|Compound:4013930|FD-Score:4.13|P-value:2.72E-7 ID:SGTC_2016|Compound:5121758|FD-Score:-3.72|P-value:2.30E-6 ID:SGTC_2059|Compound:5238311|FD-Score:-2.84|P-value:2.32E-4 ID:SGTC_2293|Compound:7624732|FD-Score:-3.04|P-value:8.77E-5 ID:SGTC_2325|Compound:6158266|FD-Score:-2.72|P-value:3.90E-4 ID:SGTC_2351|Compound:9026858|FD-Score:-2.51|P-value:9.77E-4 ID:SGTC_2390|Compound:9097792|FD-Score:-2.97|P-value:1.28E-4 ID:SGTC_2424|Compound:5537928|FD-Score:2.95|P-value:1.78E-4 ID:SGTC_2459|Compound:5811157|FD-Score:-2.78|P-value:3.02E-4 ID:SGTC_2468|Compound:5987765|FD-Score:3.12|P-value:8.07E-5 ID:SGTC_2532|Compound:xanthone|FD-Score:3.44|P-value:1.52E-5 ID:SGTC_2602|Compound:kawain|FD-Score:3.74|P-value:2.88E-6 ID:SGTC_2686|Compound:alizarin|FD-Score:-2.65|P-value:5.42E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:-3.53|P-value:6.91E-6 ID:SGTC_2695|Compound:st077686|FD-Score:2.70|P-value:5.44E-4 ID:SGTC_2711|Compound:st079103|FD-Score:2.58|P-value:9.17E-4 ID:SGTC_3039|Compound:9090270|FD-Score:3.20|P-value:5.23E-5 ID:SGTC_3080|Compound:9117754|FD-Score:2.79|P-value:3.72E-4 ID:SGTC_3159|Compound:9100096|FD-Score:-3.26|P-value:3.03E-5 ID:SGTC_3178|Compound:9107506|FD-Score:2.60|P-value:8.23E-4 ID:SGTC_3286|Compound:9117135|FD-Score:-3.02|P-value:9.69E-5 ID:SGTC_163|Compound:1327-0036|FD-Score:2.64|P-value:6.99E-4 ID:SGTC_166|Compound:k072-0058|FD-Score:-4.18|P-value:1.36E-7 ID:SGTC_230|Compound:MPP+|FD-Score:-2.51|P-value:9.74E-4 ID:SGTC_238|Compound:5hr heat shock (37°C) + nsc-638432|FD-Score:-2.58|P-value:7.30E-4 ID:SGTC_374|Compound:0880-0231|FD-Score:3.68|P-value:4.13E-6 ID:SGTC_380|Compound:3970-0968|FD-Score:3.67|P-value:4.26E-6 ID:SGTC_448|Compound:monastrol|FD-Score:3.82|P-value:1.85E-6 ID:SGTC_460|Compound:trequinsin|FD-Score:3.05|P-value:1.11E-4 ID:SGTC_517|Compound:1365-0151|FD-Score:-3.46|P-value:1.03E-5 ID:SGTC_518|Compound:1021-0119|FD-Score:-3.16|P-value:5.03E-5 ID:SGTC_686|Compound:1319-0111|FD-Score:2.75|P-value:4.38E-4 ID:SGTC_1110|Compound:3-hydroxy-3'-methoxyflavone|FD-Score:2.80|P-value:3.51E-4 ID:SGTC_1272|Compound:0839-0021|FD-Score:3.29|P-value:3.36E-5 ID:SGTC_1274|Compound:0865-0114|FD-Score:2.62|P-value:7.60E-4 ID:SGTC_1678|Compound:st019265|FD-Score:3.48|P-value:1.26E-5 ID:SGTC_2013|Compound:4024628|FD-Score:3.84|P-value:1.59E-6 ID:SGTC_2014|Compound:4013930|FD-Score:4.13|P-value:2.72E-7 ID:SGTC_2016|Compound:5121758|FD-Score:-3.72|P-value:2.30E-6 ID:SGTC_2059|Compound:5238311|FD-Score:-2.84|P-value:2.32E-4 ID:SGTC_2293|Compound:7624732|FD-Score:-3.04|P-value:8.77E-5 ID:SGTC_2325|Compound:6158266|FD-Score:-2.72|P-value:3.90E-4 ID:SGTC_2351|Compound:9026858|FD-Score:-2.51|P-value:9.77E-4 ID:SGTC_2390|Compound:9097792|FD-Score:-2.97|P-value:1.28E-4 ID:SGTC_2424|Compound:5537928|FD-Score:2.95|P-value:1.78E-4 ID:SGTC_2459|Compound:5811157|FD-Score:-2.78|P-value:3.02E-4 ID:SGTC_2468|Compound:5987765|FD-Score:3.12|P-value:8.07E-5 ID:SGTC_2532|Compound:xanthone|FD-Score:3.44|P-value:1.52E-5 ID:SGTC_2602|Compound:kawain|FD-Score:3.74|P-value:2.88E-6 ID:SGTC_2686|Compound:alizarin|FD-Score:-2.65|P-value:5.42E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:-3.53|P-value:6.91E-6 ID:SGTC_2695|Compound:st077686|FD-Score:2.70|P-value:5.44E-4 ID:SGTC_2711|Compound:st079103|FD-Score:2.58|P-value:9.17E-4 ID:SGTC_3039|Compound:9090270|FD-Score:3.20|P-value:5.23E-5 ID:SGTC_3080|Compound:9117754|FD-Score:2.79|P-value:3.72E-4 ID:SGTC_3159|Compound:9100096|FD-Score:-3.26|P-value:3.03E-5 ID:SGTC_3178|Compound:9107506|FD-Score:2.60|P-value:8.23E-4 ID:SGTC_3286|Compound:9117135|FD-Score:-3.02|P-value:9.69E-5

Top fitness defect scores for YFL003C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2014 4013930 4.13 200.0 µM 2.72E-7 25 1.82E-5
2 SGTC_2013 4024628 3.84 200.0 µM 1.59E-6 44 6.13E-5
3 SGTC_448 monastrol 3.82 171.0 µM 1.85E-6 11 6.79E-5
4 SGTC_2602 kawain 3.74 100.0 µM 2.88E-6 35 9.22E-5
5 SGTC_374 0880-0231 3.68 82.3 µM 4.13E-6 iron homeostasis 27 1.18E-4
6 SGTC_380 3970-0968 3.67 6.0 µM 4.26E-6 20 1.20E-4
7 SGTC_1678 st019265 3.48 80.2 µM 1.26E-5 60S ribosome export 35 2.55E-4
8 SGTC_2532 xanthone 3.44 78.9 µM 1.52E-5 46 2.89E-4
9 SGTC_1272 0839-0021 3.29 27.0 µM 3.36E-5 38 5.00E-4
10 SGTC_3039 9090270 3.20 49.5 µM 5.23E-5 RPP1 & pyrimidine depletion 29 6.80E-4
11 SGTC_2468 5987765 3.12 72.9 µM 8.07E-5 46 9.17E-4
12 SGTC_460 trequinsin 3.05 123.0 µM 1.11E-4 12 0.00114
13 SGTC_2424 5537928 2.95 59.2 µM 1.78E-4 96 0.00159
14 SGTC_1110 3-hydroxy-3'-methoxyflavone 2.80 1.2 µM 3.51E-4 iron homeostasis 118 0.00254
15 SGTC_3080 9117754 2.79 49.5 µM 3.72E-4 72 0.00265
16 SGTC_686 1319-0111 2.75 510.0 µM 4.38E-4 60S ribosome export 86 0.00297
17 SGTC_2695 st077686 2.70 52.0 µM 5.44E-4 105 0.00345
18 SGTC_163 1327-0036 2.64 7.5 µM 6.99E-4 244 0.00411
19 SGTC_1274 0865-0114 2.62 173.0 µM 7.60E-4 89 0.00436
20 SGTC_3178 9107506 2.60 49.5 µM 8.23E-4 92 0.00461

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.142 1.50E-16 YPL004C LSP1 hom Primary component of eisosomes, which are large immobile patch structures at the cell cortex associated with endocytosis, along with Pil1p and Sur7p; null mutants show activation of Pkc1p/Ypk1p stress resistance pathways; member of the BAR domain family
0.141 2.91E-16 YDR374C_p YDR374C_p hom Putative protein of unknown function
0.137 1.79E-15 YGR149W_p YGR149W_p hom Putative protein of unknown function; predicted to be an integal membrane protein
0.131 3.35E-14 YDR097C MSH6 hom Protein required for mismatch repair in mitosis and meiosis, forms a complex with Msh2p to repair both single-base & insertion-deletion mispairs; also involved in interstrand cross-link repair; potentially phosphorylated by Cdc28p
0.126 2.19E-13 YHL040C ARN1 hom ARN family transporter for siderophore-iron chelates; responsible for uptake of iron bound to ferrirubin, ferrirhodin, and related siderophores; protein increases in abundance and relocalizes to the vacuole upon DNA replication stress
0.121 2.05E-12 YEL001C IRC22 hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion localizes to the ER; YEL001C is non-essential; null mutant displays increased levels of spontaneous Rad52p foci
0.121 2.49E-12 YDR133C_d YDR133C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps YDR134C
0.120 3.15E-12 YBL019W APN2 hom Class II abasic (AP) endonuclease involved in repair of DNA damage; homolog of human HAP1 and E. coli exoIII
0.118 7.67E-12 YFL063W_d YFL063W_d hom Dubious open reading frame, based on available experimental and comparative sequence data
0.114 3.48E-11 YLR413W_p YLR413W_p hom Putative protein of unknown function; YLR413W is not an essential gene
0.114 4.06E-11 YFR015C GSY1 hom Glycogen synthase; expression induced by glucose limitation, nitrogen starvation, environmental stress, and entry into stationary phase; GSY1 has a paralog, GSY2, that arose from the whole genome duplication; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
0.107 5.85E-10 YKL105C SEG2 hom Eisosome component and paralog of Seg1p; likely plays only minor role in eisosome assembly; shown to interact with Seg1p; similar to A. gossypii SEG gene which is important for stabilizing eisosomes
0.105 1.22E-9 YFL014W HSP12 hom Plasma membrane protein involved in maintaining membrane organization in stress conditions; induced by heat shock, oxidative stress, osmostress, stationary phase, glucose depletion, oleate and alcohol; protein abundance increased in response to DNA replication stress and dietary restriction; regulated by the HOG and Ras-Pka pathways; required for dietary restriction-induced lifespan extension
0.105 1.27E-9 YKL164C PIR1 hom O-glycosylated protein required for cell wall stability; attached to the cell wall via beta-1,3-glucan; mediates mitochondrial translocation of Apn1p; expression regulated by the cell integrity pathway and by Swi5p during the cell cycle; PIR1 has a paralog, YJL160C, that arose from the whole genome duplication
0.104 1.34E-9 YML047C PRM6 hom Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; regulated by Ste12p during mating; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family