YFL004W / VTC2

Subunit of the vacuolar transporter chaperone (VTC) complex involved in membrane trafficking, vacuolar polyphosphate accumulation, microautophagy and non-autophagic vacuolar fusion

Zygosity: Homozygous strain
fixedexpanded
Profile for YFL004W / VTC2

Click on Significant Values for Screen Details ID:SGTC_160|Compound:3453-0671|FD-Score:-3.75|P-value:5.45E-5 ID:SGTC_163|Compound:1327-0036|FD-Score:4.87|P-value:2.42E-7 ID:SGTC_358|Compound:0075-0058|FD-Score:3.45|P-value:1.81E-4 ID:SGTC_423|Compound:5-fluorouracil|FD-Score:3.31|P-value:3.20E-4 ID:SGTC_429|Compound:1112-0049|FD-Score:3.78|P-value:4.73E-5 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:3.34|P-value:2.79E-4 ID:SGTC_516|Compound:3992-2719|FD-Score:-3.58|P-value:1.07E-4 ID:SGTC_539|Compound:4859-0462|FD-Score:3.02|P-value:9.02E-4 ID:SGTC_543|Compound:3844-1095|FD-Score:3.88|P-value:3.01E-5 ID:SGTC_550|Compound:4123-0385|FD-Score:4.62|P-value:9.16E-7 ID:SGTC_707|Compound:0865-0008|FD-Score:3.62|P-value:9.14E-5 ID:SGTC_953|Compound:1188-0045|FD-Score:3.39|P-value:2.36E-4 ID:SGTC_965|Compound:1291-5240|FD-Score:5.57|P-value:4.43E-9 ID:SGTC_1205|Compound:4161-2785|FD-Score:-5.57|P-value:4.41E-9 ID:SGTC_1210|Compound:3013-0140|FD-Score:3.25|P-value:3.95E-4 ID:SGTC_1324|Compound:1418-0026|FD-Score:4.32|P-value:3.97E-6 ID:SGTC_1371|Compound:2421-0008|FD-Score:-3.43|P-value:2.00E-4 ID:SGTC_1379|Compound:2144-0755|FD-Score:5.29|P-value:2.30E-8 ID:SGTC_1462|Compound:k081-0014|FD-Score:-3.02|P-value:8.90E-4 ID:SGTC_1578|Compound:josamycin|FD-Score:-3.07|P-value:7.67E-4 ID:SGTC_1810|Compound:st050138|FD-Score:3.69|P-value:6.86E-5 ID:SGTC_2178|Compound:5812956|FD-Score:-3.02|P-value:9.11E-4 ID:SGTC_2260|Compound:7964312|FD-Score:3.04|P-value:8.56E-4 ID:SGTC_2306|Compound:7482397|FD-Score:3.88|P-value:3.14E-5 ID:SGTC_2979|Compound:9012552|FD-Score:-3.77|P-value:4.83E-5 ID:SGTC_2984|Compound:9022109|FD-Score:-3.16|P-value:5.54E-4 ID:SGTC_2987|Compound:9018338|FD-Score:-3.05|P-value:8.01E-4 ID:SGTC_3023|Compound:9089636|FD-Score:-3.03|P-value:8.86E-4 ID:SGTC_3034|Compound:9090421|FD-Score:3.70|P-value:6.59E-5 ID:SGTC_3042|Compound:9091578|FD-Score:3.18|P-value:5.17E-4 ID:SGTC_160|Compound:3453-0671|FD-Score:-3.75|P-value:5.45E-5 ID:SGTC_163|Compound:1327-0036|FD-Score:4.87|P-value:2.42E-7 ID:SGTC_358|Compound:0075-0058|FD-Score:3.45|P-value:1.81E-4 ID:SGTC_423|Compound:5-fluorouracil|FD-Score:3.31|P-value:3.20E-4 ID:SGTC_429|Compound:1112-0049|FD-Score:3.78|P-value:4.73E-5 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:3.34|P-value:2.79E-4 ID:SGTC_516|Compound:3992-2719|FD-Score:-3.58|P-value:1.07E-4 ID:SGTC_539|Compound:4859-0462|FD-Score:3.02|P-value:9.02E-4 ID:SGTC_543|Compound:3844-1095|FD-Score:3.88|P-value:3.01E-5 ID:SGTC_550|Compound:4123-0385|FD-Score:4.62|P-value:9.16E-7 ID:SGTC_707|Compound:0865-0008|FD-Score:3.62|P-value:9.14E-5 ID:SGTC_953|Compound:1188-0045|FD-Score:3.39|P-value:2.36E-4 ID:SGTC_965|Compound:1291-5240|FD-Score:5.57|P-value:4.43E-9 ID:SGTC_1205|Compound:4161-2785|FD-Score:-5.57|P-value:4.41E-9 ID:SGTC_1210|Compound:3013-0140|FD-Score:3.25|P-value:3.95E-4 ID:SGTC_1324|Compound:1418-0026|FD-Score:4.32|P-value:3.97E-6 ID:SGTC_1371|Compound:2421-0008|FD-Score:-3.43|P-value:2.00E-4 ID:SGTC_1379|Compound:2144-0755|FD-Score:5.29|P-value:2.30E-8 ID:SGTC_1462|Compound:k081-0014|FD-Score:-3.02|P-value:8.90E-4 ID:SGTC_1578|Compound:josamycin|FD-Score:-3.07|P-value:7.67E-4 ID:SGTC_1810|Compound:st050138|FD-Score:3.69|P-value:6.86E-5 ID:SGTC_2178|Compound:5812956|FD-Score:-3.02|P-value:9.11E-4 ID:SGTC_2260|Compound:7964312|FD-Score:3.04|P-value:8.56E-4 ID:SGTC_2306|Compound:7482397|FD-Score:3.88|P-value:3.14E-5 ID:SGTC_2979|Compound:9012552|FD-Score:-3.77|P-value:4.83E-5 ID:SGTC_2984|Compound:9022109|FD-Score:-3.16|P-value:5.54E-4 ID:SGTC_2987|Compound:9018338|FD-Score:-3.05|P-value:8.01E-4 ID:SGTC_3023|Compound:9089636|FD-Score:-3.03|P-value:8.86E-4 ID:SGTC_3034|Compound:9090421|FD-Score:3.70|P-value:6.59E-5 ID:SGTC_3042|Compound:9091578|FD-Score:3.18|P-value:5.17E-4

Top fitness defect scores for YFL004W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_965 1291-5240 5.57 308.0 µM 4.43E-9 10 1.29E-8
2 SGTC_1379 2144-0755 5.29 36.5 µM 2.30E-8 8 6.04E-8
3 SGTC_163 1327-0036 4.87 7.5 µM 2.42E-7 68 5.51E-7
4 SGTC_550 4123-0385 4.62 25.7 µM 9.16E-7 TSC3-RPN4 13 1.92E-6
5 SGTC_1324 1418-0026 4.32 34.4 µM 3.97E-6 7 7.63E-6
6 SGTC_543 3844-1095 3.88 191.0 µM 3.01E-5 ERG2 52 5.12E-5
7 SGTC_2306 7482397 3.88 179.5 µM 3.14E-5 RPP1 & pyrimidine depletion 21 5.33E-5
8 SGTC_429 1112-0049 3.78 25.5 µM 4.73E-5 DNA damage response 37 7.85E-5
9 SGTC_3034 9090421 3.70 49.5 µM 6.59E-5 60S ribosome export 10 1.07E-4
10 SGTC_1810 st050138 3.69 53.0 µM 6.86E-5 PDR1 18 1.11E-4
11 SGTC_707 0865-0008 3.62 204.0 µM 9.14E-5 DNA damage response 9 1.46E-4
12 SGTC_358 0075-0058 3.45 9.4 µM 1.81E-4 88 2.77E-4
13 SGTC_953 1188-0045 3.39 9.7 µM 2.36E-4 60S ribosome export 47 3.56E-4
14 SGTC_453 enantio-paf c16 3.34 10.0 µM 2.79E-4 204 4.17E-4
15 SGTC_423 5-fluorouracil 3.31 457.0 µM 3.20E-4 exosome 46 4.73E-4
16 SGTC_1210 3013-0140 3.25 750.0 nM 3.95E-4 22 5.78E-4
17 SGTC_3042 9091578 3.18 49.5 µM 5.17E-4 ERG2 18 7.44E-4
18 SGTC_2260 7964312 3.04 200.0 µM 8.56E-4 47 0.00120
19 SGTC_539 4859-0462 3.02 2.5 µM 9.02E-4 RNA pol III & RNase P/MRP 60 0.00126
20 SGTC_3021 9087658 2.98 49.5 µM 0.00105 27 0.00145

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.175 2.54E-24 YGR193C PDX1 hom Dihydrolipoamide dehydrogenase (E3)-binding protein (E3BP) of the mitochondrial pyruvate dehydrogenase (PDH) complex, plays a structural role in the complex by binding and positioning E3 to the dihydrolipoamide acetyltransferase (E2) core
0.150 2.14E-18 YML101C-A_d YML101C-A_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.141 1.91E-16 YOL071W EMI5 hom Subunit of succinate dehydrogenase, which couples succinate oxidation to ubiquinone reduction; required for FAD cofactor attachment to Sdh1p; mutations in human ortholog PGL2 are associated with neuroendocrine tumors (paraganglioma)
0.135 3.61E-15 YJR013W GPI14 het Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M
0.132 2.17E-14 YIR008C PRI1 het Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair
0.126 2.74E-13 YGR161W-C_p YGR161W-C_p hom Putative protein of unknown function; identified by sequence comparison with hemiascomycetous yeast species
0.121 2.07E-12 YOL019W_p YOL019W_p hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and vacuole; YOL019W has a paralog, DCV1, that arose from the whole genome duplication
0.119 4.43E-12 YER052C HOM3 hom Aspartate kinase (L-aspartate 4-P-transferase); cytoplasmic enzyme that catalyzes the first step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis
0.110 1.74E-10 YFL037W TUB2 het Beta-tubulin; associates with alpha-tubulin (Tub1p and Tub3p) to form tubulin dimer, which polymerizes to form microtubules; mutation in human ortholog is associated with congenital fibrosis of the extraocular muscles (CFEOM) with polymicrogyria
0.110 1.95E-10 YEL001C IRC22 hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion localizes to the ER; YEL001C is non-essential; null mutant displays increased levels of spontaneous Rad52p foci
0.107 5.82E-10 YOL024W_p YOL024W_p hom Putative protein of unknown function; predicted to have thiol-disulfide oxidoreductase active site; YOL024W has a paralog, IGD1, that arose from the whole genome duplication
0.106 6.35E-10 YDR295C HDA2 hom Subunit of a possibly tetrameric trichostatin A-sensitive class II histone deacetylase complex containing an Hda1p homodimer and an Hda2p-Hda3p heterodimer; involved in telomere maintenance
0.101 4.06E-9 YER089C PTC2 hom Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p to limit maximal osmostress induced kinase activity; dephosphorylates Ire1p to downregulate the unfolded protein response; dephosphorylates Cdc28p; inactivates the DNA damage checkpoint; PTC2 has a paralog, PTC3, that arose from the whole genome duplication
0.101 4.38E-9 YDR403W DIT1 hom Sporulation-specific enzyme required for spore wall maturation, involved in the production of a soluble LL-dityrosine-containing precursor of the spore wall; transcripts accumulate at the time of prospore enclosure
0.101 5.37E-9 YDR381W YRA1 het RNA binding protein required for export of poly(A)+ mRNA from the nucleus; proposed to couple mRNA export with 3' end processing via its interactions with Mex67p and Pcf11p; functionally redundant with Yra2p, another REF family member