YFL005W / SEC4

Rab family GTPase essential for vesicle-mediated exocytic secretion and autophagy; associates with the exocyst component Sec15p and may regulate polarized delivery of transport vesicles to the exocyst at the plasma membrane

Zygosity: Heterozygous strain
fixedexpanded
Profile for YFL005W / SEC4

Click on Significant Values for Screen Details ID:SGTC_57|Compound:miconazole|FD-Score:2.93|P-value:8.17E-4 ID:SGTC_66|Compound:0109-0187|FD-Score:-4.67|P-value:1.64E-7 ID:SGTC_74|Compound:000s-0487|FD-Score:-2.96|P-value:5.86E-4 ID:SGTC_108|Compound:0509-0257|FD-Score:3.65|P-value:4.35E-5 ID:SGTC_143|Compound:4032-1194|FD-Score:6.05|P-value:3.31E-11 ID:SGTC_151|Compound:3013-0144|FD-Score:-3.57|P-value:4.71E-5 ID:SGTC_157|Compound:1348-1695|FD-Score:-3.16|P-value:2.64E-4 ID:SGTC_197|Compound:4544-0043|FD-Score:5.57|P-value:8.91E-10 ID:SGTC_252|Compound:miconazole|FD-Score:5.55|P-value:1.04E-9 ID:SGTC_256|Compound:clotrimazole|FD-Score:6.74|P-value:1.61E-13 ID:SGTC_312|Compound:4092-0653|FD-Score:7.97|P-value:3.47E-18 ID:SGTC_320|Compound:4092-0846|FD-Score:6.27|P-value:6.50E-12 ID:SGTC_330|Compound:1488-0319|FD-Score:-3.63|P-value:3.48E-5 ID:SGTC_433|Compound:pimozide|FD-Score:2.92|P-value:8.70E-4 ID:SGTC_515|Compound:4092-1064|FD-Score:7.67|P-value:5.37E-17 ID:SGTC_528|Compound:3492-0019|FD-Score:3.44|P-value:1.06E-4 ID:SGTC_549|Compound:1661-1316|FD-Score:4.23|P-value:2.54E-6 ID:SGTC_550|Compound:4123-0385|FD-Score:3.91|P-value:1.26E-5 ID:SGTC_562|Compound:3772-2733|FD-Score:3.04|P-value:5.49E-4 ID:SGTC_684|Compound:1140-0066|FD-Score:5.15|P-value:1.36E-8 ID:SGTC_685|Compound:1278-0033|FD-Score:4.74|P-value:1.57E-7 ID:SGTC_689|Compound:0833-0039|FD-Score:3.46|P-value:1.01E-4 ID:SGTC_704|Compound:1310-0270|FD-Score:2.96|P-value:7.30E-4 ID:SGTC_748|Compound:0136-0228|FD-Score:3.67|P-value:3.89E-5 ID:SGTC_752|Compound:1896-0090|FD-Score:-2.93|P-value:6.41E-4 ID:SGTC_836|Compound:0330-0104|FD-Score:-3.41|P-value:9.28E-5 ID:SGTC_844|Compound:0330-0132|FD-Score:7.14|P-value:5.84E-15 ID:SGTC_898|Compound:1000-0849|FD-Score:2.96|P-value:7.36E-4 ID:SGTC_976|Compound:1348-1067|FD-Score:3.44|P-value:1.08E-4 ID:SGTC_1072|Compound:oxiconazole|FD-Score:3.73|P-value:2.96E-5 ID:SGTC_1119|Compound:parthenolide|FD-Score:5.85|P-value:1.30E-10 ID:SGTC_1123|Compound:mangostin|FD-Score:-2.98|P-value:5.38E-4 ID:SGTC_1282|Compound:0886-0010|FD-Score:3.01|P-value:6.10E-4 ID:SGTC_1286|Compound:0929-0070|FD-Score:-3.89|P-value:1.02E-5 ID:SGTC_1289|Compound:0976-0073|FD-Score:4.03|P-value:7.15E-6 ID:SGTC_1353|Compound:1498-1206|FD-Score:-2.99|P-value:5.22E-4 ID:SGTC_1467|Compound:k284-1082|FD-Score:3.94|P-value:1.12E-5 ID:SGTC_1470|Compound:k295-0292|FD-Score:3.74|P-value:2.80E-5 ID:SGTC_1499|Compound:4480-0877|FD-Score:3.37|P-value:1.43E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:9.20|P-value:1.30E-23 ID:SGTC_1599|Compound:8-chlro-1-tetrahydronorharmanone|FD-Score:-3.31|P-value:1.46E-4 ID:SGTC_1605|Compound:st000082|FD-Score:3.84|P-value:1.79E-5 ID:SGTC_1621|Compound:st003705|FD-Score:3.57|P-value:6.17E-5 ID:SGTC_1626|Compound:st003712|FD-Score:3.06|P-value:5.12E-4 ID:SGTC_1679|Compound:4130-1276|FD-Score:4.53|P-value:5.27E-7 ID:SGTC_1690|Compound:st023502|FD-Score:3.41|P-value:1.23E-4 ID:SGTC_1704|Compound:st028805|FD-Score:3.04|P-value:5.52E-4 ID:SGTC_1712|Compound:st032288|FD-Score:4.62|P-value:3.09E-7 ID:SGTC_1713|Compound:st033146|FD-Score:2.93|P-value:8.35E-4 ID:SGTC_1714|Compound:st033236|FD-Score:3.00|P-value:6.43E-4 ID:SGTC_1724|Compound:st037281|FD-Score:-3.62|P-value:3.70E-5 ID:SGTC_1844|Compound:rapamycin|FD-Score:3.11|P-value:4.10E-4 ID:SGTC_1975|Compound:st070967|FD-Score:5.04|P-value:2.79E-8 ID:SGTC_1976|Compound:st070609|FD-Score:3.67|P-value:3.99E-5 ID:SGTC_2036|Compound:5160441|FD-Score:4.60|P-value:3.56E-7 ID:SGTC_2038|Compound:4100038|FD-Score:4.14|P-value:4.20E-6 ID:SGTC_2106|Compound:5357045|FD-Score:4.79|P-value:1.22E-7 ID:SGTC_2249|Compound:haloperidol|FD-Score:5.00|P-value:3.54E-8 ID:SGTC_2250|Compound:alverine citrate|FD-Score:3.46|P-value:9.98E-5 ID:SGTC_2297|Compound:7971671|FD-Score:3.48|P-value:9.14E-5 ID:SGTC_2301|Compound:7769476|FD-Score:3.19|P-value:3.08E-4 ID:SGTC_2303|Compound:7774051|FD-Score:3.60|P-value:5.47E-5 ID:SGTC_2306|Compound:7482397|FD-Score:3.05|P-value:5.28E-4 ID:SGTC_2313|Compound:7726885|FD-Score:-2.96|P-value:5.89E-4 ID:SGTC_2374|Compound:9071832|FD-Score:3.79|P-value:2.30E-5 ID:SGTC_2397|Compound:5161689|FD-Score:3.39|P-value:1.35E-4 ID:SGTC_2402|Compound:5553605|FD-Score:-3.20|P-value:2.27E-4 ID:SGTC_2451|Compound:5856223|FD-Score:-4.04|P-value:4.92E-6 ID:SGTC_2492|Compound:derrusnin|FD-Score:3.35|P-value:1.57E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:3.61|P-value:5.13E-5 ID:SGTC_2694|Compound:st077685|FD-Score:-2.82|P-value:9.74E-4 ID:SGTC_2778|Compound:tioconazole|FD-Score:5.05|P-value:2.58E-8 ID:SGTC_2858|Compound:9025781|FD-Score:3.58|P-value:5.94E-5 ID:SGTC_2905|Compound:7799065|FD-Score:3.22|P-value:2.65E-4 ID:SGTC_2908|Compound:7930301|FD-Score:3.54|P-value:6.88E-5 ID:SGTC_2996|Compound:9066192|FD-Score:-3.39|P-value:1.03E-4 ID:SGTC_3060|Compound:1327-0027|FD-Score:3.04|P-value:5.41E-4 ID:SGTC_3082|Compound:9117008|FD-Score:-3.10|P-value:3.43E-4 ID:SGTC_3098|Compound:9119039|FD-Score:5.03|P-value:2.97E-8 ID:SGTC_3182|Compound:9108849|FD-Score:3.41|P-value:1.24E-4 ID:SGTC_3219|Compound:9131112|FD-Score:3.93|P-value:1.14E-5 ID:SGTC_3220|Compound:9131269|FD-Score:3.58|P-value:5.99E-5 ID:SGTC_57|Compound:miconazole|FD-Score:2.93|P-value:8.17E-4 ID:SGTC_66|Compound:0109-0187|FD-Score:-4.67|P-value:1.64E-7 ID:SGTC_74|Compound:000s-0487|FD-Score:-2.96|P-value:5.86E-4 ID:SGTC_108|Compound:0509-0257|FD-Score:3.65|P-value:4.35E-5 ID:SGTC_143|Compound:4032-1194|FD-Score:6.05|P-value:3.31E-11 ID:SGTC_151|Compound:3013-0144|FD-Score:-3.57|P-value:4.71E-5 ID:SGTC_157|Compound:1348-1695|FD-Score:-3.16|P-value:2.64E-4 ID:SGTC_197|Compound:4544-0043|FD-Score:5.57|P-value:8.91E-10 ID:SGTC_252|Compound:miconazole|FD-Score:5.55|P-value:1.04E-9 ID:SGTC_256|Compound:clotrimazole|FD-Score:6.74|P-value:1.61E-13 ID:SGTC_312|Compound:4092-0653|FD-Score:7.97|P-value:3.47E-18 ID:SGTC_320|Compound:4092-0846|FD-Score:6.27|P-value:6.50E-12 ID:SGTC_330|Compound:1488-0319|FD-Score:-3.63|P-value:3.48E-5 ID:SGTC_433|Compound:pimozide|FD-Score:2.92|P-value:8.70E-4 ID:SGTC_515|Compound:4092-1064|FD-Score:7.67|P-value:5.37E-17 ID:SGTC_528|Compound:3492-0019|FD-Score:3.44|P-value:1.06E-4 ID:SGTC_549|Compound:1661-1316|FD-Score:4.23|P-value:2.54E-6 ID:SGTC_550|Compound:4123-0385|FD-Score:3.91|P-value:1.26E-5 ID:SGTC_562|Compound:3772-2733|FD-Score:3.04|P-value:5.49E-4 ID:SGTC_684|Compound:1140-0066|FD-Score:5.15|P-value:1.36E-8 ID:SGTC_685|Compound:1278-0033|FD-Score:4.74|P-value:1.57E-7 ID:SGTC_689|Compound:0833-0039|FD-Score:3.46|P-value:1.01E-4 ID:SGTC_704|Compound:1310-0270|FD-Score:2.96|P-value:7.30E-4 ID:SGTC_748|Compound:0136-0228|FD-Score:3.67|P-value:3.89E-5 ID:SGTC_752|Compound:1896-0090|FD-Score:-2.93|P-value:6.41E-4 ID:SGTC_836|Compound:0330-0104|FD-Score:-3.41|P-value:9.28E-5 ID:SGTC_844|Compound:0330-0132|FD-Score:7.14|P-value:5.84E-15 ID:SGTC_898|Compound:1000-0849|FD-Score:2.96|P-value:7.36E-4 ID:SGTC_976|Compound:1348-1067|FD-Score:3.44|P-value:1.08E-4 ID:SGTC_1072|Compound:oxiconazole|FD-Score:3.73|P-value:2.96E-5 ID:SGTC_1119|Compound:parthenolide|FD-Score:5.85|P-value:1.30E-10 ID:SGTC_1123|Compound:mangostin|FD-Score:-2.98|P-value:5.38E-4 ID:SGTC_1282|Compound:0886-0010|FD-Score:3.01|P-value:6.10E-4 ID:SGTC_1286|Compound:0929-0070|FD-Score:-3.89|P-value:1.02E-5 ID:SGTC_1289|Compound:0976-0073|FD-Score:4.03|P-value:7.15E-6 ID:SGTC_1353|Compound:1498-1206|FD-Score:-2.99|P-value:5.22E-4 ID:SGTC_1467|Compound:k284-1082|FD-Score:3.94|P-value:1.12E-5 ID:SGTC_1470|Compound:k295-0292|FD-Score:3.74|P-value:2.80E-5 ID:SGTC_1499|Compound:4480-0877|FD-Score:3.37|P-value:1.43E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:9.20|P-value:1.30E-23 ID:SGTC_1599|Compound:8-chlro-1-tetrahydronorharmanone|FD-Score:-3.31|P-value:1.46E-4 ID:SGTC_1605|Compound:st000082|FD-Score:3.84|P-value:1.79E-5 ID:SGTC_1621|Compound:st003705|FD-Score:3.57|P-value:6.17E-5 ID:SGTC_1626|Compound:st003712|FD-Score:3.06|P-value:5.12E-4 ID:SGTC_1679|Compound:4130-1276|FD-Score:4.53|P-value:5.27E-7 ID:SGTC_1690|Compound:st023502|FD-Score:3.41|P-value:1.23E-4 ID:SGTC_1704|Compound:st028805|FD-Score:3.04|P-value:5.52E-4 ID:SGTC_1712|Compound:st032288|FD-Score:4.62|P-value:3.09E-7 ID:SGTC_1713|Compound:st033146|FD-Score:2.93|P-value:8.35E-4 ID:SGTC_1714|Compound:st033236|FD-Score:3.00|P-value:6.43E-4 ID:SGTC_1724|Compound:st037281|FD-Score:-3.62|P-value:3.70E-5 ID:SGTC_1844|Compound:rapamycin|FD-Score:3.11|P-value:4.10E-4 ID:SGTC_1975|Compound:st070967|FD-Score:5.04|P-value:2.79E-8 ID:SGTC_1976|Compound:st070609|FD-Score:3.67|P-value:3.99E-5 ID:SGTC_2036|Compound:5160441|FD-Score:4.60|P-value:3.56E-7 ID:SGTC_2038|Compound:4100038|FD-Score:4.14|P-value:4.20E-6 ID:SGTC_2106|Compound:5357045|FD-Score:4.79|P-value:1.22E-7 ID:SGTC_2249|Compound:haloperidol|FD-Score:5.00|P-value:3.54E-8 ID:SGTC_2250|Compound:alverine citrate|FD-Score:3.46|P-value:9.98E-5 ID:SGTC_2297|Compound:7971671|FD-Score:3.48|P-value:9.14E-5 ID:SGTC_2301|Compound:7769476|FD-Score:3.19|P-value:3.08E-4 ID:SGTC_2303|Compound:7774051|FD-Score:3.60|P-value:5.47E-5 ID:SGTC_2306|Compound:7482397|FD-Score:3.05|P-value:5.28E-4 ID:SGTC_2313|Compound:7726885|FD-Score:-2.96|P-value:5.89E-4 ID:SGTC_2374|Compound:9071832|FD-Score:3.79|P-value:2.30E-5 ID:SGTC_2397|Compound:5161689|FD-Score:3.39|P-value:1.35E-4 ID:SGTC_2402|Compound:5553605|FD-Score:-3.20|P-value:2.27E-4 ID:SGTC_2451|Compound:5856223|FD-Score:-4.04|P-value:4.92E-6 ID:SGTC_2492|Compound:derrusnin|FD-Score:3.35|P-value:1.57E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:3.61|P-value:5.13E-5 ID:SGTC_2694|Compound:st077685|FD-Score:-2.82|P-value:9.74E-4 ID:SGTC_2778|Compound:tioconazole|FD-Score:5.05|P-value:2.58E-8 ID:SGTC_2858|Compound:9025781|FD-Score:3.58|P-value:5.94E-5 ID:SGTC_2905|Compound:7799065|FD-Score:3.22|P-value:2.65E-4 ID:SGTC_2908|Compound:7930301|FD-Score:3.54|P-value:6.88E-5 ID:SGTC_2996|Compound:9066192|FD-Score:-3.39|P-value:1.03E-4 ID:SGTC_3060|Compound:1327-0027|FD-Score:3.04|P-value:5.41E-4 ID:SGTC_3082|Compound:9117008|FD-Score:-3.10|P-value:3.43E-4 ID:SGTC_3098|Compound:9119039|FD-Score:5.03|P-value:2.97E-8 ID:SGTC_3182|Compound:9108849|FD-Score:3.41|P-value:1.24E-4 ID:SGTC_3219|Compound:9131112|FD-Score:3.93|P-value:1.14E-5 ID:SGTC_3220|Compound:9131269|FD-Score:3.58|P-value:5.99E-5

Top fitness defect scores for YFL005W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_1597 7-benzyloxygramine 9.20 47.5 µM 1.30E-23 calcium & mitochondrial duress 19 1.87E-20 0.14
2 SGTC_312 4092-0653 7.97 6.8 µM 3.47E-18 ergosterol depletion effects on membrane 9 8.03E-16 0.14
3 SGTC_515 4092-1064 7.67 102.0 µM 5.37E-17 plasma membrane duress 4 8.31E-15 0.43
4 SGTC_844 0330-0132 7.14 15.3 µM 5.84E-15 1 4.57E-13 1.85
5 SGTC_256 clotrimazole 6.74 625.0 nM 1.61E-13 ergosterol depletion effects on membrane 6 7.77E-12 0.12
6 SGTC_320 4092-0846 6.27 5.3 µM 6.50E-12 ergosterol depletion effects on membrane 7 1.84E-10 0.33
7 SGTC_143 4032-1194 6.05 15.7 µM 3.31E-11 3 7.41E-10 0.43
8 SGTC_1119 parthenolide 5.85 318.5 nM 1.30E-10 TSC3-RPN4 4 2.40E-9 1.14
9 SGTC_197 4544-0043 5.57 41.6 µM 8.91E-10 endomembrane recycling 12 1.24E-8 0.08
10 SGTC_252 miconazole 5.55 1.3 nM 1.04E-9 ergosterol depletion effects on membrane 8 1.42E-8 0.04
11 SGTC_684 1140-0066 5.15 328.0 µM 1.36E-8 Golgi 2 1.28E-7 1.18
12 SGTC_2778 tioconazole 5.05 65.1 nM 2.58E-8 ergosterol depletion effects on membrane 18 2.23E-7 0.10
13 SGTC_1975 st070967 5.04 30.8 µM 2.79E-8 4 2.39E-7 0.08
14 SGTC_3098 9119039 5.03 49.5 µM 2.97E-8 Golgi 6 2.51E-7 0.83
15 SGTC_2249 haloperidol 5.00 116.4 µM 3.54E-8 ergosterol depletion effects on membrane 23 2.93E-7 0.18
16 SGTC_2106 5357045 4.79 167.3 µM 1.22E-7 4 8.47E-7 1.04
17 SGTC_685 1278-0033 4.74 54.9 µM 1.57E-7 60S ribosome export 5 1.05E-6 0.54
18 SGTC_1712 st032288 4.62 53.4 µM 3.09E-7 calcium & mitochondrial duress 27 1.88E-6 0.10
19 SGTC_2036 5160441 4.60 105.0 µM 3.56E-7 NEO1 21 2.12E-6 0.09
20 SGTC_1679 4130-1276 4.53 6.7 µM 5.27E-7 Golgi 21 2.97E-6 0.10

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.277 3.08E-60 YPR055W SEC8 het Essential 121 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in ER and Golgi inheritance in small buds; relocalizes away from bud neck upon DNA replication stress
0.254 1.26E-50 YBR102C EXO84 het Exocyst subunit with dual roles in exocytosis and spliceosome assembly; subunit of the the exocyst complex which mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane (PM) prior to SNARE-mediated fusion; required for exocyst assembly and targeting the complex to specific sites on the bud tip PM; associates the U1 snRNP; role in pre-mRNA splicing and prespliceosome formation; possible Cdc28 substrate
0.239 7.79E-45 YIL068C SEC6 het Essential 88kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; anchors the assembled complex to sites of secretion; interacts with SM-like protein and SNARE regulator Sec1p and may recruit it to sites of secretion; interacts with Sec9p and inhibits formation of the t-SNARE complex between Sec9p and Sso1p
0.233 1.18E-42 YOL081W IRA2 hom GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions; similar to human neurofibromin; IRA2 has a paralog, IRA1, that arose from the whole genome duplication
0.232 3.84E-42 YKL051W SFK1 hom Plasma membrane protein that may act together with or upstream of Stt4p to generate normal levels of the essential phospholipid PI4P, at least partially mediates proper localization of Stt4p to the plasma membrane
0.231 7.77E-42 YGL233W SEC15 het Essential 113 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; interacts with and functions as a downstream effector of active, GTP-bound Sec4p, a Rab family GTPase
0.228 8.26E-41 YBL010C_p YBL010C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein colocalizes with clathrin-coated vesicles
0.223 5.72E-39 YJL085W EXO70 het Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in an actin-independent manner, targeting and anchoring the exocyst with Sec3p; involved in exocyst assembly; direct downstream effector of Rho3p and Cdc42p; relocalizes from bud neck to cytoplasm upon DNA replication stress
0.222 1.44E-38 YFL034W_p YFL034W_p hom Putative integral membrane protein that interacts with Rpp0p, which is a component of the ribosomal stalk
0.218 2.71E-37 YAL038W CDC19 het Pyruvate kinase; functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration; regulated via allosteric activation by fructose bisphosphate; CDC19 has a paralog, PYK2, that arose from the whole genome duplication
0.199 2.26E-31 YGR253C PUP2 het Alpha 5 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit zeta
0.198 5.87E-31 YJL165C HAL5 hom Putative protein kinase; overexpression increases sodium and lithium tolerance, whereas gene disruption increases cation and low pH sensitivity and impairs potassium uptake, suggesting a role in regulation of Trk1p and/or Trk2p transporters; HAL5 has a paralog, KKQ8, that arose from the whole genome duplication
0.196 3.01E-30 YMR218C TRS130 het One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in ER to Golgi membrane traffic; mutation activates transcription of OCH1; mutants are defective in cytoplasm-to-vacuole targeting (Cvt) pathway and starvation-induced autophagy
0.190 8.93E-29 YPR091C NVJ2 hom Lipid-binding ER protein, enriched at nucleus-vacuolar junctions (NVJ); may be involved in sterol metabolism or signaling at the NVJ; contains a synaptotagmin-like-mitochondrial- lipid binding protein (SMP) domain; binds phosphatidylinositols and other lipids in a large-scale study; may interact with ribosomes, based on co-purification experiments
0.189 3.19E-28 YJL129C TRK1 hom Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter; phosphorylated in vivo and interacts physically with the phosphatase Ppz1p, suggesting Trk1p acitivy is regulated by phosphorylation; TRK1 has a paralog, TRK2, that arose from the whole genome duplication