HIPHOP chemogenomics database

YFL007W / BLM10

Proteasome activator; binds the core proteasome and stimulates proteasome-mediated protein degradation by inducing gate opening; required for resistance to bleomycin, may be involved in protecting against oxidative damage; similar to mammalian PA200

Zygosity: Homozygous strain

fixedexpanded
Profile for YFL007W / BLM10

Click on Significant Values for Screen Details

Top fitness defect scores for YFL007W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_1882	5615643	6.74	20.0 µM	3.59E-12	TRP & mitochondrial translation	14	7.87E-12
2	SGTC_2778	tioconazole	5.79	65.1 nM	2.03E-9	ergosterol depletion effects on membrane	32	3.57E-9
3	SGTC_1854	st056233	5.29	32.9 µM	3.81E-8	amide catabolism	6	6.03E-8
4	SGTC_2688	kanamycin b	5.14	96.7 µM	8.96E-8	calcium & mitochondrial duress	232	1.38E-7
5	SGTC_1745	st081495	4.76	74.0 µM	6.74E-7	RNA processing & uracil transport	47	9.68E-7
6	SGTC_1190	1486-1332	4.49	13.0 µM	2.55E-6		8	3.51E-6
7	SGTC_213	1246-0773	4.37	967.0 nM	4.50E-6		15	6.08E-6
8	SGTC_37	0566-0070	4.24	1.6 µM	8.43E-6		21	1.11E-5
9	SGTC_1247	0448-0043	4.23	5.8 µM	8.86E-6	ERG2	30	1.17E-5
10	SGTC_1712	st032288	4.15	53.4 µM	1.29E-5	calcium & mitochondrial duress	178	1.68E-5
11	SGTC_1977	st070720	3.90	65.3 µM	3.79E-5		10	4.77E-5
12	SGTC_1874	staurosporine	3.81	7.6 µM	5.70E-5	cell wall signaling	31	7.08E-5
13	SGTC_1169	aniline yellow	3.76	25.8 µM	6.83E-5		14	8.43E-5
14	SGTC_382	3229-0966	3.76	23.2 µM	6.95E-5		27	8.57E-5
15	SGTC_1876	myriocin	3.73	605.0 nM	7.77E-5		41	9.55E-5
16	SGTC_2698	st077697	3.65	67.5 µM	1.07E-4		21	1.31E-4
17	SGTC_3304	9125678	3.60	46.1 µM	1.30E-4		66	1.57E-4
18	SGTC_1526	st051518	3.49	75.0 µM	2.06E-4		35	2.45E-4
19	SGTC_3014	9081533	3.40	49.5 µM	2.85E-4		17	3.37E-4
20	SGTC_1218	1348-1416	3.39	30.7 µM	2.94E-4	SWF1 & branched chain AA biosynthesis	32	3.47E-4

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.124	5.76E-13	YBL067C	UBP13	hom	Putative ubiquitin-specific protease that cleaves Ub-protein fusions; UBP13 has a paralog, UBP9, that arose from the whole genome duplication
0.122	1.13E-12	YMR099C	YMR099C	hom	Glucose-6-phosphate 1-epimerase (hexose-6-phosphate mutarotase), likely involved in carbohydrate metabolism; GFP-fusion protein localizes to both the nucleus and cytoplasm and is induced in response to the DNA-damaging agent MMS
0.122	1.48E-12	YBR287W_p	YBR287W_p	hom	Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the ER; YBR287W is not an essential gene
0.120	3.56E-12	YGR212W	SLI1	hom	N-acetyltransferase, confers resistance to the sphingolipid biosynthesis inhibitor myriocin (ISP-1) by converting it into N-acetyl-myriocin, co-operates with Ypk1p in mediating resistance to myriocin
0.117	9.31E-12	YDL019C	OSH2	hom	Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability
0.109	2.52E-10	YKL051W	SFK1	hom	Plasma membrane protein that may act together with or upstream of Stt4p to generate normal levels of the essential phospholipid PI4P, at least partially mediates proper localization of Stt4p to the plasma membrane
0.107	4.36E-10	YDR467C_d	YDR467C_d	hom	Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.105	1.18E-9	YKL058W	TOA2	het	TFIIA small subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to smallest subunit of human and Drosophila TFIIA; protein abundance increases in response to DNA replication stress
0.103	2.05E-9	YCL021W-A_p	YCL021W-A_p	hom	Putative protein of unknown function
0.101	4.39E-9	YDR349C	YPS7	hom	Putative GPI-anchored aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; located in the cytoplasm and endoplasmic reticulum
0.098	1.41E-8	YKL019W	RAM2	het	Alpha subunit of both the farnesyltransferase and type I geranylgeranyltransferase that catalyze prenylation of proteins containing a CAAX consensus motif; essential protein required for membrane localization of Ras proteins and a-factor
0.094	5.59E-8	YGL079W_p	KXD1_p	hom	Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; null mutant is sensitive to drug inducing secretion of vacuolar cargo; GFP-fusion protein localizes to the endosome
0.093	6.33E-8	YMR296C	LCB1	het	Component of serine palmitoyltransferase, responsible along with Lcb2p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine
0.093	6.94E-8	YBR197C	YBR197C	hom	Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR197C is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress; YBR197C has a paralog, YPL077C, that arose from the whole genome duplication
0.089	2.68E-7	YGL155W	CDC43	het	Beta subunit of geranylgeranyltransferase type I, catalyzes geranylgeranylation to the cysteine residue in proteins containing a C-terminal CaaX sequence ending in Leu or Phe; has substrates important for morphogenesis