YFL009W / CDC4

F-box protein required for G1/S and G2/M transition, associates with Skp1p and Cdc53p to form a complex, SCFCdc4, which acts as ubiquitin-protein ligase directing ubiquitination of the phosphorylated CDK inhibitor Sic1p

Zygosity: Heterozygous strain
fixedexpanded
Profile for YFL009W / CDC4

Click on Significant Values for Screen Details ID:SGTC_73|Compound:0327-0325|FD-Score:-7.17|P-value:6.72E-15 ID:SGTC_173|Compound:1273-0061|FD-Score:-3.83|P-value:2.03E-5 ID:SGTC_251|Compound:melphalan|FD-Score:2.87|P-value:9.61E-4 ID:SGTC_344|Compound:k064-0035|FD-Score:2.93|P-value:7.58E-4 ID:SGTC_406|Compound:estrone|FD-Score:3.09|P-value:4.09E-4 ID:SGTC_431|Compound:k089-0097|FD-Score:-3.16|P-value:3.60E-4 ID:SGTC_527|Compound:4476-4215|FD-Score:-2.90|P-value:9.63E-4 ID:SGTC_528|Compound:3492-0019|FD-Score:2.90|P-value:8.45E-4 ID:SGTC_530|Compound:4358-1714|FD-Score:3.89|P-value:1.34E-5 ID:SGTC_606|Compound:k015-0008|FD-Score:3.26|P-value:2.07E-4 ID:SGTC_762|Compound:1165-0429|FD-Score:3.07|P-value:4.56E-4 ID:SGTC_958|Compound:1222-0007|FD-Score:2.94|P-value:7.45E-4 ID:SGTC_999|Compound:4022-0066|FD-Score:-3.04|P-value:5.61E-4 ID:SGTC_1006|Compound:4099-6547|FD-Score:-3.25|P-value:2.49E-4 ID:SGTC_1240|Compound:0356-0016|FD-Score:-3.69|P-value:3.73E-5 ID:SGTC_1347|Compound:1493-0251|FD-Score:-3.62|P-value:5.29E-5 ID:SGTC_1377|Compound:2944-0135|FD-Score:-3.65|P-value:4.48E-5 ID:SGTC_1514|Compound:1189-1591|FD-Score:2.95|P-value:7.00E-4 ID:SGTC_1522|Compound:st012842|FD-Score:-3.20|P-value:3.00E-4 ID:SGTC_1573|Compound:oleylamine|FD-Score:-3.19|P-value:3.10E-4 ID:SGTC_1580|Compound:hydrocortisone 21-hemisuccinate|FD-Score:5.42|P-value:2.36E-9 ID:SGTC_1589|Compound:2',5'-dimethoxyflavone|FD-Score:-3.25|P-value:2.52E-4 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:-3.71|P-value:3.41E-5 ID:SGTC_1787|Compound:fluconazole|FD-Score:-6.02|P-value:5.05E-11 ID:SGTC_1814|Compound:st052110|FD-Score:3.21|P-value:2.56E-4 ID:SGTC_2096|Compound:5468907|FD-Score:2.88|P-value:9.28E-4 ID:SGTC_2126|Compound:5251174|FD-Score:3.56|P-value:5.87E-5 ID:SGTC_2127|Compound:5253358|FD-Score:3.04|P-value:5.05E-4 ID:SGTC_2241|Compound:6772625|FD-Score:-3.26|P-value:2.35E-4 ID:SGTC_2410|Compound:aloe-emodin-8-o-glycoside|FD-Score:2.90|P-value:8.44E-4 ID:SGTC_2549|Compound:cedrol|FD-Score:3.90|P-value:1.28E-5 ID:SGTC_2607|Compound:solasodine|FD-Score:-3.77|P-value:2.69E-5 ID:SGTC_2616|Compound:abietic acid|FD-Score:3.67|P-value:3.71E-5 ID:SGTC_2749|Compound:bexarotene|FD-Score:3.64|P-value:4.22E-5 ID:SGTC_2755|Compound:lomerizine|FD-Score:-4.43|P-value:9.92E-7 ID:SGTC_2760|Compound:etidronate|FD-Score:-3.59|P-value:5.87E-5 ID:SGTC_2787|Compound:5284540|FD-Score:3.11|P-value:3.77E-4 ID:SGTC_2792|Compound:7535235|FD-Score:-3.84|P-value:1.94E-5 ID:SGTC_2794|Compound:7785382|FD-Score:6.08|P-value:2.73E-11 ID:SGTC_2803|Compound:7707263|FD-Score:-2.94|P-value:8.24E-4 ID:SGTC_73|Compound:0327-0325|FD-Score:-7.17|P-value:6.72E-15 ID:SGTC_173|Compound:1273-0061|FD-Score:-3.83|P-value:2.03E-5 ID:SGTC_251|Compound:melphalan|FD-Score:2.87|P-value:9.61E-4 ID:SGTC_344|Compound:k064-0035|FD-Score:2.93|P-value:7.58E-4 ID:SGTC_406|Compound:estrone|FD-Score:3.09|P-value:4.09E-4 ID:SGTC_431|Compound:k089-0097|FD-Score:-3.16|P-value:3.60E-4 ID:SGTC_527|Compound:4476-4215|FD-Score:-2.90|P-value:9.63E-4 ID:SGTC_528|Compound:3492-0019|FD-Score:2.90|P-value:8.45E-4 ID:SGTC_530|Compound:4358-1714|FD-Score:3.89|P-value:1.34E-5 ID:SGTC_606|Compound:k015-0008|FD-Score:3.26|P-value:2.07E-4 ID:SGTC_762|Compound:1165-0429|FD-Score:3.07|P-value:4.56E-4 ID:SGTC_958|Compound:1222-0007|FD-Score:2.94|P-value:7.45E-4 ID:SGTC_999|Compound:4022-0066|FD-Score:-3.04|P-value:5.61E-4 ID:SGTC_1006|Compound:4099-6547|FD-Score:-3.25|P-value:2.49E-4 ID:SGTC_1240|Compound:0356-0016|FD-Score:-3.69|P-value:3.73E-5 ID:SGTC_1347|Compound:1493-0251|FD-Score:-3.62|P-value:5.29E-5 ID:SGTC_1377|Compound:2944-0135|FD-Score:-3.65|P-value:4.48E-5 ID:SGTC_1514|Compound:1189-1591|FD-Score:2.95|P-value:7.00E-4 ID:SGTC_1522|Compound:st012842|FD-Score:-3.20|P-value:3.00E-4 ID:SGTC_1573|Compound:oleylamine|FD-Score:-3.19|P-value:3.10E-4 ID:SGTC_1580|Compound:hydrocortisone 21-hemisuccinate|FD-Score:5.42|P-value:2.36E-9 ID:SGTC_1589|Compound:2',5'-dimethoxyflavone|FD-Score:-3.25|P-value:2.52E-4 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:-3.71|P-value:3.41E-5 ID:SGTC_1787|Compound:fluconazole|FD-Score:-6.02|P-value:5.05E-11 ID:SGTC_1814|Compound:st052110|FD-Score:3.21|P-value:2.56E-4 ID:SGTC_2096|Compound:5468907|FD-Score:2.88|P-value:9.28E-4 ID:SGTC_2126|Compound:5251174|FD-Score:3.56|P-value:5.87E-5 ID:SGTC_2127|Compound:5253358|FD-Score:3.04|P-value:5.05E-4 ID:SGTC_2241|Compound:6772625|FD-Score:-3.26|P-value:2.35E-4 ID:SGTC_2410|Compound:aloe-emodin-8-o-glycoside|FD-Score:2.90|P-value:8.44E-4 ID:SGTC_2549|Compound:cedrol|FD-Score:3.90|P-value:1.28E-5 ID:SGTC_2607|Compound:solasodine|FD-Score:-3.77|P-value:2.69E-5 ID:SGTC_2616|Compound:abietic acid|FD-Score:3.67|P-value:3.71E-5 ID:SGTC_2749|Compound:bexarotene|FD-Score:3.64|P-value:4.22E-5 ID:SGTC_2755|Compound:lomerizine|FD-Score:-4.43|P-value:9.92E-7 ID:SGTC_2760|Compound:etidronate|FD-Score:-3.59|P-value:5.87E-5 ID:SGTC_2787|Compound:5284540|FD-Score:3.11|P-value:3.77E-4 ID:SGTC_2792|Compound:7535235|FD-Score:-3.84|P-value:1.94E-5 ID:SGTC_2794|Compound:7785382|FD-Score:6.08|P-value:2.73E-11 ID:SGTC_2803|Compound:7707263|FD-Score:-2.94|P-value:8.24E-4

Top fitness defect scores for YFL009W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2794 7785382 6.08 27.3 µM 2.73E-11 3 6.15E-10 0.04
2 SGTC_1580 hydrocortisone 21-hemisuccinate 5.42 43.2 µM 2.36E-9 1 2.90E-8 0.89
3 SGTC_2549 cedrol 3.90 20.9 µM 1.28E-5 10 4.91E-5 0.05
4 SGTC_530 4358-1714 3.89 28.0 µM 1.34E-5 6 5.11E-5 0.05
5 SGTC_2616 abietic acid 3.67 100.0 µM 3.71E-5 6 1.23E-4 0.00
6 SGTC_2749 bexarotene 3.64 57.4 µM 4.22E-5 8 1.38E-4 0.04
7 SGTC_2126 5251174 3.56 33.9 µM 5.87E-5 13 1.84E-4 0.02
8 SGTC_606 k015-0008 3.26 38.6 µM 2.07E-4 6 5.47E-4 0.10
9 SGTC_1814 st052110 3.21 31.5 µM 2.56E-4 RNA processing & uracil transport 21 6.59E-4 0.06
10 SGTC_2787 5284540 3.11 71.4 µM 3.77E-4 19 9.22E-4 8.24E-4
11 SGTC_406 estrone 3.09 100.0 µM 4.09E-4 7 9.90E-4 0.03
12 SGTC_762 1165-0429 3.07 115.0 µM 4.56E-4 TSC3-RPN4 13 0.00109 5.62E-4
13 SGTC_2127 5253358 3.04 187.9 µM 5.05E-4 16 0.00119 0.04
14 SGTC_1514 1189-1591 2.95 158.0 µM 7.00E-4 15 0.00158 0.05
15 SGTC_958 1222-0007 2.94 33.0 µM 7.45E-4 20 0.00166 0.02
16 SGTC_344 k064-0035 2.93 6.4 µM 7.58E-4 60S ribosome export 14 0.00169 0.01
17 SGTC_2410 aloe-emodin-8-o-glycoside 2.90 46.3 µM 8.44E-4 mitochondrial processes 19 0.00185 0.03
18 SGTC_528 3492-0019 2.90 172.0 µM 8.45E-4 calcium & mitochondrial duress 42 0.00186 0.07
19 SGTC_2096 5468907 2.88 200.0 µM 9.28E-4 8 0.00201 0.05
20 SGTC_251 melphalan 2.87 456.5 µM 9.61E-4 DNA damage response 16 0.00208 0.00

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.151 1.24E-18 YNL312W RFA2 het Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination; protein abundance increases in response to DNA replication stress
0.147 1.52E-17 YMR132C_p JLP2_p hom Protein of unknown function, contains sequence that closely resembles a J domain (typified by the E. coli DnaJ protein)
0.130 4.18E-14 YOR084W LPX1 hom Oleic acid-inducible, peroxisomal matrix localized lipase; transcriptionally activated by Yrm1p along with genes involved in multidrug resistance; peroxisomal import is dependent on the PTS1 receptor, Pex5p and on self-interaction
0.119 4.50E-12 YFR014C CMK1 hom Calmodulin-dependent protein kinase; may play a role in stress response, many Ca++/calmodulin dependent phosphorylation substrates demonstrated in vitro, amino acid sequence similar to mammalian Cam Kinase II; CMK1 has a paralog, CMK2, that arose from the whole genome duplication
0.117 8.56E-12 YDR327W_d YDR327W_d het Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene SKP1
0.115 2.13E-11 YOL139C CDC33 het mRNA cap binding protein and translation initiation factor eIF4E; the eIF4E-cap complex is responsible for mediating cap-dependent mRNA translation via interactions with translation initiation factor eIF4G (Tif4631p or Tif4632p); protein abundance increases in response to DNA replication stress; mutants are defective for adhesion and pseudohyphal growth
0.115 2.39E-11 YLR117C CLF1 het Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; homolog of Drosophila crooked neck protein; interacts with U1 snRNP proteins
0.111 1.35E-10 YOR374W ALD4 hom Mitochondrial aldehyde dehydrogenase, required for growth on ethanol and conversion of acetaldehyde to acetate; phosphorylated; activity is K+ dependent; utilizes NADP+ or NAD+ equally as coenzymes; expression is glucose repressed
0.109 2.42E-10 YKR022C NTR2 het Essential protein that forms a dimer with Ntr1p; also forms a trimer, with Ntr2p and the DExD/H-box RNA helicase Prp43p, that is involved in spliceosome disassembly
0.107 5.43E-10 YJL064W_d YJL064W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene YJL065C/DLS1
0.104 1.43E-9 YDR531W CAB1 het Pantothenate kinase (ATP:D-pantothenate 4'-phosphotransferase, EC 2.7.1.33); catalyzes the first committed step in the universal biosynthetic pathway for synthesis of coenzyme A (CoA); transcriptionally regulated by Upc2p via a sterol response element
0.102 3.07E-9 YJR016C ILV3 het Dihydroxyacid dehydratase, catalyzes third step in the common pathway leading to biosynthesis of branched-chain amino acids
0.101 4.05E-9 YDR328C SKP1 het Evolutionarily conserved kinetochore protein; part of multiple protein complexes, including the SCF ubiquitin ligase complex, the CBF3 complex that binds centromeric DNA, and the RAVE complex that regulates assembly of the V-ATPase; protein abundance increases in response to DNA replication stress
0.101 4.22E-9 YOR087W YVC1 hom Vacuolar cation channel, mediates release of Ca(2+) from the vacuole in response to hyperosmotic shock
0.100 5.78E-9 YMR112C MED11 het Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential protein