YFL016C / MDJ1

Co-chaperone that stimulates the ATPase activity of the HSP70 protein Ssc1p; involved in protein folding/refolding in the mitochodrial matrix; required for proteolysis of misfolded proteins; member of the HSP40 (DnaJ) family of chaperones

Zygosity: Homozygous strain
fixedexpanded
Profile for YFL016C / MDJ1

Click on Significant Values for Screen Details ID:SGTC_34|Compound:0469-0683|FD-Score:4.63|P-value:2.79E-5 ID:SGTC_36|Compound:0335-0881|FD-Score:6.28|P-value:1.72E-8 ID:SGTC_59|Compound:0330-0160|FD-Score:4.24|P-value:1.14E-4 ID:SGTC_63|Compound:0099-0242|FD-Score:7.14|P-value:1.52E-10 ID:SGTC_64|Compound:000l-0018|FD-Score:6.52|P-value:4.79E-9 ID:SGTC_67|Compound:0335-0847|FD-Score:5.47|P-value:8.37E-7 ID:SGTC_70|Compound:0141-0289|FD-Score:5.77|P-value:2.09E-7 ID:SGTC_86|Compound:0417-1630|FD-Score:8.46|P-value:3.67E-14 ID:SGTC_100|Compound:0086-0128|FD-Score:4.22|P-value:1.24E-4 ID:SGTC_116|Compound:0155-0182|FD-Score:9.77|P-value:2.23E-18 ID:SGTC_119|Compound:0121-0043|FD-Score:4.46|P-value:5.19E-5 ID:SGTC_134|Compound:0269-0018|FD-Score:4.63|P-value:2.76E-5 ID:SGTC_137|Compound:0438-0332|FD-Score:4.32|P-value:8.53E-5 ID:SGTC_141|Compound:0443-0274|FD-Score:4.58|P-value:3.30E-5 ID:SGTC_142|Compound:0335-0849|FD-Score:3.98|P-value:2.84E-4 ID:SGTC_158|Compound:k038-0087|FD-Score:5.54|P-value:6.09E-7 ID:SGTC_173|Compound:1273-0061|FD-Score:5.41|P-value:1.09E-6 ID:SGTC_200|Compound:k038-0079|FD-Score:4.85|P-value:1.16E-5 ID:SGTC_227|Compound:fluconazole|FD-Score:4.05|P-value:2.25E-4 ID:SGTC_236|Compound:1509-0011|FD-Score:9.14|P-value:2.90E-16 ID:SGTC_251|Compound:melphalan|FD-Score:7.18|P-value:1.25E-10 ID:SGTC_275|Compound:trichlorophene|FD-Score:7.59|P-value:1.06E-11 ID:SGTC_386|Compound:1509-0011|FD-Score:4.80|P-value:1.39E-5 ID:SGTC_430|Compound:0958-0115|FD-Score:3.82|P-value:4.75E-4 ID:SGTC_525|Compound:k781-2675|FD-Score:4.03|P-value:2.41E-4 ID:SGTC_582|Compound:0987-0078|FD-Score:3.65|P-value:8.10E-4 ID:SGTC_778|Compound:3456-2434|FD-Score:8.20|P-value:2.13E-13 ID:SGTC_831|Compound:k061-1017|FD-Score:4.25|P-value:1.12E-4 ID:SGTC_927|Compound:2425-1185|FD-Score:6.69|P-value:1.92E-9 ID:SGTC_1036|Compound:k216-0215|FD-Score:6.37|P-value:1.06E-8 ID:SGTC_1044|Compound:1222-0021|FD-Score:3.87|P-value:4.02E-4 ID:SGTC_1093|Compound:fluphenazine|FD-Score:3.73|P-value:6.38E-4 ID:SGTC_1145|Compound:3546-0597|FD-Score:5.48|P-value:8.01E-7 ID:SGTC_1159|Compound:4092-0538|FD-Score:3.89|P-value:3.81E-4 ID:SGTC_1227|Compound:3909-8010|FD-Score:4.22|P-value:1.22E-4 ID:SGTC_1317|Compound:1273-0028|FD-Score:4.03|P-value:2.40E-4 ID:SGTC_1353|Compound:1498-1206|FD-Score:4.54|P-value:3.92E-5 ID:SGTC_1413|Compound:3910-0535|FD-Score:3.66|P-value:7.93E-4 ID:SGTC_1635|Compound:st007035|FD-Score:4.40|P-value:6.51E-5 ID:SGTC_1717|Compound:st033143|FD-Score:-3.75|P-value:2.25E-4 ID:SGTC_1718|Compound:roemerine|FD-Score:-3.30|P-value:9.47E-4 ID:SGTC_1723|Compound:st034307|FD-Score:3.61|P-value:9.31E-4 ID:SGTC_1854|Compound:st056233|FD-Score:3.97|P-value:2.89E-4 ID:SGTC_1861|Compound:st057647|FD-Score:-4.46|P-value:1.68E-5 ID:SGTC_1960|Compound:st011366|FD-Score:3.59|P-value:9.71E-4 ID:SGTC_2003|Compound:4023581|FD-Score:-4.21|P-value:4.31E-5 ID:SGTC_2029|Compound:5175081|FD-Score:4.06|P-value:2.19E-4 ID:SGTC_2037|Compound:5169046|FD-Score:3.99|P-value:2.71E-4 ID:SGTC_2250|Compound:alverine citrate|FD-Score:3.71|P-value:6.63E-4 ID:SGTC_2309|Compound:7664088|FD-Score:-5.25|P-value:5.92E-7 ID:SGTC_2509|Compound:methyl 7-deshydroxypyrogallin-4-carboxylate|FD-Score:3.73|P-value:6.25E-4 ID:SGTC_2513|Compound:baicalein|FD-Score:5.12|P-value:3.81E-6 ID:SGTC_2582|Compound:agaric acid|FD-Score:-3.42|P-value:6.51E-4 ID:SGTC_2650|Compound:prasterone|FD-Score:-4.57|P-value:1.05E-5 ID:SGTC_2651|Compound:1327-0036|FD-Score:-4.75|P-value:5.04E-6 ID:SGTC_2672|Compound:12a-hydroxy-5-deoxydehydromunduserone|FD-Score:3.98|P-value:2.81E-4 ID:SGTC_2759|Compound:disulfiram|FD-Score:4.52|P-value:4.23E-5 ID:SGTC_2802|Compound:7925815|FD-Score:3.86|P-value:4.16E-4 ID:SGTC_2840|Compound:9008771|FD-Score:-3.30|P-value:9.55E-4 ID:SGTC_2943|Compound:9068529|FD-Score:6.32|P-value:1.37E-8 ID:SGTC_2965|Compound:9082772|FD-Score:5.09|P-value:4.26E-6 ID:SGTC_3049|Compound:9092223|FD-Score:4.12|P-value:1.77E-4 ID:SGTC_3062|Compound:9077494|FD-Score:5.40|P-value:1.14E-6 ID:SGTC_3195|Compound:9113082|FD-Score:5.51|P-value:6.80E-7 ID:SGTC_3196|Compound:9111891|FD-Score:5.24|P-value:2.27E-6 ID:SGTC_3299|Compound:9121982|FD-Score:4.33|P-value:8.49E-5 ID:SGTC_34|Compound:0469-0683|FD-Score:4.63|P-value:2.79E-5 ID:SGTC_36|Compound:0335-0881|FD-Score:6.28|P-value:1.72E-8 ID:SGTC_59|Compound:0330-0160|FD-Score:4.24|P-value:1.14E-4 ID:SGTC_63|Compound:0099-0242|FD-Score:7.14|P-value:1.52E-10 ID:SGTC_64|Compound:000l-0018|FD-Score:6.52|P-value:4.79E-9 ID:SGTC_67|Compound:0335-0847|FD-Score:5.47|P-value:8.37E-7 ID:SGTC_70|Compound:0141-0289|FD-Score:5.77|P-value:2.09E-7 ID:SGTC_86|Compound:0417-1630|FD-Score:8.46|P-value:3.67E-14 ID:SGTC_100|Compound:0086-0128|FD-Score:4.22|P-value:1.24E-4 ID:SGTC_116|Compound:0155-0182|FD-Score:9.77|P-value:2.23E-18 ID:SGTC_119|Compound:0121-0043|FD-Score:4.46|P-value:5.19E-5 ID:SGTC_134|Compound:0269-0018|FD-Score:4.63|P-value:2.76E-5 ID:SGTC_137|Compound:0438-0332|FD-Score:4.32|P-value:8.53E-5 ID:SGTC_141|Compound:0443-0274|FD-Score:4.58|P-value:3.30E-5 ID:SGTC_142|Compound:0335-0849|FD-Score:3.98|P-value:2.84E-4 ID:SGTC_158|Compound:k038-0087|FD-Score:5.54|P-value:6.09E-7 ID:SGTC_173|Compound:1273-0061|FD-Score:5.41|P-value:1.09E-6 ID:SGTC_200|Compound:k038-0079|FD-Score:4.85|P-value:1.16E-5 ID:SGTC_227|Compound:fluconazole|FD-Score:4.05|P-value:2.25E-4 ID:SGTC_236|Compound:1509-0011|FD-Score:9.14|P-value:2.90E-16 ID:SGTC_251|Compound:melphalan|FD-Score:7.18|P-value:1.25E-10 ID:SGTC_275|Compound:trichlorophene|FD-Score:7.59|P-value:1.06E-11 ID:SGTC_386|Compound:1509-0011|FD-Score:4.80|P-value:1.39E-5 ID:SGTC_430|Compound:0958-0115|FD-Score:3.82|P-value:4.75E-4 ID:SGTC_525|Compound:k781-2675|FD-Score:4.03|P-value:2.41E-4 ID:SGTC_582|Compound:0987-0078|FD-Score:3.65|P-value:8.10E-4 ID:SGTC_778|Compound:3456-2434|FD-Score:8.20|P-value:2.13E-13 ID:SGTC_831|Compound:k061-1017|FD-Score:4.25|P-value:1.12E-4 ID:SGTC_927|Compound:2425-1185|FD-Score:6.69|P-value:1.92E-9 ID:SGTC_1036|Compound:k216-0215|FD-Score:6.37|P-value:1.06E-8 ID:SGTC_1044|Compound:1222-0021|FD-Score:3.87|P-value:4.02E-4 ID:SGTC_1093|Compound:fluphenazine|FD-Score:3.73|P-value:6.38E-4 ID:SGTC_1145|Compound:3546-0597|FD-Score:5.48|P-value:8.01E-7 ID:SGTC_1159|Compound:4092-0538|FD-Score:3.89|P-value:3.81E-4 ID:SGTC_1227|Compound:3909-8010|FD-Score:4.22|P-value:1.22E-4 ID:SGTC_1317|Compound:1273-0028|FD-Score:4.03|P-value:2.40E-4 ID:SGTC_1353|Compound:1498-1206|FD-Score:4.54|P-value:3.92E-5 ID:SGTC_1413|Compound:3910-0535|FD-Score:3.66|P-value:7.93E-4 ID:SGTC_1635|Compound:st007035|FD-Score:4.40|P-value:6.51E-5 ID:SGTC_1717|Compound:st033143|FD-Score:-3.75|P-value:2.25E-4 ID:SGTC_1718|Compound:roemerine|FD-Score:-3.30|P-value:9.47E-4 ID:SGTC_1723|Compound:st034307|FD-Score:3.61|P-value:9.31E-4 ID:SGTC_1854|Compound:st056233|FD-Score:3.97|P-value:2.89E-4 ID:SGTC_1861|Compound:st057647|FD-Score:-4.46|P-value:1.68E-5 ID:SGTC_1960|Compound:st011366|FD-Score:3.59|P-value:9.71E-4 ID:SGTC_2003|Compound:4023581|FD-Score:-4.21|P-value:4.31E-5 ID:SGTC_2029|Compound:5175081|FD-Score:4.06|P-value:2.19E-4 ID:SGTC_2037|Compound:5169046|FD-Score:3.99|P-value:2.71E-4 ID:SGTC_2250|Compound:alverine citrate|FD-Score:3.71|P-value:6.63E-4 ID:SGTC_2309|Compound:7664088|FD-Score:-5.25|P-value:5.92E-7 ID:SGTC_2509|Compound:methyl 7-deshydroxypyrogallin-4-carboxylate|FD-Score:3.73|P-value:6.25E-4 ID:SGTC_2513|Compound:baicalein|FD-Score:5.12|P-value:3.81E-6 ID:SGTC_2582|Compound:agaric acid|FD-Score:-3.42|P-value:6.51E-4 ID:SGTC_2650|Compound:prasterone|FD-Score:-4.57|P-value:1.05E-5 ID:SGTC_2651|Compound:1327-0036|FD-Score:-4.75|P-value:5.04E-6 ID:SGTC_2672|Compound:12a-hydroxy-5-deoxydehydromunduserone|FD-Score:3.98|P-value:2.81E-4 ID:SGTC_2759|Compound:disulfiram|FD-Score:4.52|P-value:4.23E-5 ID:SGTC_2802|Compound:7925815|FD-Score:3.86|P-value:4.16E-4 ID:SGTC_2840|Compound:9008771|FD-Score:-3.30|P-value:9.55E-4 ID:SGTC_2943|Compound:9068529|FD-Score:6.32|P-value:1.37E-8 ID:SGTC_2965|Compound:9082772|FD-Score:5.09|P-value:4.26E-6 ID:SGTC_3049|Compound:9092223|FD-Score:4.12|P-value:1.77E-4 ID:SGTC_3062|Compound:9077494|FD-Score:5.40|P-value:1.14E-6 ID:SGTC_3195|Compound:9113082|FD-Score:5.51|P-value:6.80E-7 ID:SGTC_3196|Compound:9111891|FD-Score:5.24|P-value:2.27E-6 ID:SGTC_3299|Compound:9121982|FD-Score:4.33|P-value:8.49E-5

Top fitness defect scores for YFL016C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_116 0155-0182 9.77 31.8 µM 2.23E-18 mitochondrial response to ROS 5 7.23E-23
2 SGTC_236 1509-0011 9.14 5.3 µM 2.90E-16 213 3.17E-20
3 SGTC_86 0417-1630 8.46 471.9 µM 3.67E-14 ubiquinone biosynthesis & proteasome 54 1.35E-17
4 SGTC_778 3456-2434 8.20 196.0 µM 2.13E-13 mitochondrial response to ROS 7 1.22E-16
5 SGTC_275 trichlorophene 7.59 5.7 µM 1.06E-11 mitochondrial stress 82 1.63E-14
6 SGTC_251 melphalan 7.18 456.5 µM 1.25E-10 DNA damage response 4 3.59E-13
7 SGTC_63 0099-0242 7.14 26.7 µM 1.52E-10 mitochondrial stress 16 4.55E-13
8 SGTC_927 2425-1185 6.69 123.0 µM 1.92E-9 2 1.10E-11
9 SGTC_64 000l-0018 6.52 272.1 µM 4.79E-9 ubiquinone biosynthesis & proteasome 68 3.46E-11
10 SGTC_1036 k216-0215 6.37 33.5 µM 1.06E-8 mitochondrial response to ROS 13 9.40E-11
11 SGTC_2943 9068529 6.32 45.3 µM 1.37E-8 1 1.30E-10
12 SGTC_36 0335-0881 6.28 14.2 µM 1.72E-8 ubiquinone biosynthesis & proteasome 59 1.72E-10
13 SGTC_70 0141-0289 5.77 43.3 µM 2.09E-7 mitochondrial stress 89 3.96E-9
14 SGTC_158 k038-0087 5.54 26.4 µM 6.09E-7 ubiquinone biosynthesis & proteasome 79 1.52E-8
15 SGTC_3195 9113082 5.51 49.5 µM 6.80E-7 5 1.75E-8
16 SGTC_1145 3546-0597 5.48 10.2 µM 8.01E-7 mitochondrial processes 5 2.15E-8
17 SGTC_67 0335-0847 5.47 266.0 µM 8.37E-7 ubiquinone biosynthesis & proteasome 73 2.27E-8
18 SGTC_173 1273-0061 5.41 2.7 µM 1.09E-6 mitochondrial stress 128 3.15E-8
19 SGTC_3062 9077494 5.40 49.5 µM 1.14E-6 mitochondrial response to ROS 20 3.33E-8
20 SGTC_3196 9111891 5.24 49.5 µM 2.27E-6 mitochondrial processes 5 7.94E-8

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.408 1.39E-134 YDR204W COQ4 hom Protein with a role in ubiquinone (Coenzyme Q) biosynthesis, possibly functioning in stabilization of Coq7p; located on the matrix face of the mitochondrial inner membrane; component of a mitochondrial ubiquinone-synthesizing complex
0.400 2.08E-129 YOR125C CAT5 hom Protein required for ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with ubiquinone biosynthetic enzymes; required for gluconeogenic gene activation
0.393 1.61E-124 YLR201C COQ9 hom Protein required for ubiquinone (coenzyme Q) biosynthesis and respiratory growth; localizes to the matrix face of the mitochondrial inner membrane in a large complex with ubiquinone biosynthetic enzymes
0.390 2.94E-122 YGR257C MTM1 hom Mitochondrial protein of the mitochondrial carrier family, involved in activating mitochondrial Sod2p probably by facilitating insertion of an essential manganese cofactor
0.383 1.03E-117 YGL119W COQ8 hom Protein required for ubiquinone biosynthesis and respiratory growth; exhibits genetic interaction with COQ9, suggesting a common function; similar to prokaryotic proteins involved in early steps of ubiquinone biosynthesis; COQ8 has a paralog, YBR230W-A, that arose from the whole genome duplication
0.376 4.20E-113 YNR041C COQ2 hom Para hydroxybenzoate: polyprenyl transferase, catalyzes the second step in ubiquinone (coenzyme Q) biosynthesis
0.369 5.71E-109 YNR042W_d YNR042W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene COQ2
0.369 1.09E-108 YLR239C LIP2 hom Lipoyl ligase, involved in the modification of mitochondrial enzymes by the attachment of lipoic acid groups
0.368 7.21E-108 YOL096C COQ3 hom O-methyltransferase, catalyzes two different O-methylation steps in ubiquinone (Coenzyme Q) biosynthesis; component of a mitochondrial ubiquinone-synthesizing complex; phosphoprotein
0.340 1.66E-91 YOR158W PET123 hom Mitochondrial ribosomal protein of the small subunit; PET123 exhibits genetic interactions with PET122, which encodes a COX3 mRNA-specific translational activator
0.340 2.09E-91 YNL252C MRPL17 hom Mitochondrial ribosomal protein of the large subunit
0.339 6.26E-91 YLR202C_d YLR202C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YLR201C; ORF contains a putative intron
0.333 1.93E-87 YML110C COQ5 hom 2-hexaprenyl-6-methoxy-1,4-benzoquinone methyltransferase, involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes
0.332 2.59E-87 YOR196C LIP5 hom Protein involved in biosynthesis of the coenzyme lipoic acid, has similarity to E. coli lipoic acid synthase
0.329 1.08E-85 YGR255C COQ6 hom Putative flavin-dependent monooxygenase; involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes; human COX6 can rescue a yeast cox6 mutant and is implicated in steroid-resistant nephrotic syndrome (SRNS)