YFL022C / FRS2

Alpha subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs1p to form active enzyme; evolutionarily distant from mitochondrial phenylalanyl-tRNA synthetase based on protein sequence, but substrate binding is similar

Zygosity: Heterozygous strain
fixedexpanded
Profile for YFL022C / FRS2

Click on Significant Values for Screen Details ID:SGTC_36|Compound:0335-0881|FD-Score:3.78|P-value:9.06E-5 ID:SGTC_96|Compound:0929-0093|FD-Score:3.27|P-value:6.22E-4 ID:SGTC_143|Compound:4032-1194|FD-Score:7.84|P-value:3.48E-15 ID:SGTC_144|Compound:4161-2749|FD-Score:8.55|P-value:1.02E-17 ID:SGTC_145|Compound:3737-0113|FD-Score:11.60|P-value:3.47E-31 ID:SGTC_146|Compound:3448-8650|FD-Score:7.26|P-value:2.74E-13 ID:SGTC_166|Compound:k072-0058|FD-Score:3.88|P-value:6.06E-5 ID:SGTC_184|Compound:1494-0393|FD-Score:3.57|P-value:2.04E-4 ID:SGTC_608|Compound:k048-0125|FD-Score:10.30|P-value:9.44E-25 ID:SGTC_669|Compound:0108-0021|FD-Score:3.37|P-value:4.36E-4 ID:SGTC_712|Compound:r015-0009|FD-Score:6.22|P-value:3.36E-10 ID:SGTC_813|Compound:k007-0158|FD-Score:3.62|P-value:1.72E-4 ID:SGTC_858|Compound:0438-0351|FD-Score:4.25|P-value:1.31E-5 ID:SGTC_900|Compound:1112-0045|FD-Score:-4.30|P-value:8.10E-6 ID:SGTC_1187|Compound:1391-0724|FD-Score:3.50|P-value:2.64E-4 ID:SGTC_1196|Compound:1588-0294|FD-Score:4.29|P-value:1.09E-5 ID:SGTC_1248|Compound:0469-0687|FD-Score:9.55|P-value:1.15E-21 ID:SGTC_1406|Compound:3909-7906|FD-Score:3.80|P-value:8.47E-5 ID:SGTC_1417|Compound:3966-0327|FD-Score:-3.29|P-value:4.73E-4 ID:SGTC_1432|Compound:4049-0298|FD-Score:3.54|P-value:2.32E-4 ID:SGTC_1434|Compound:4073-0095|FD-Score:4.01|P-value:3.52E-5 ID:SGTC_1915|Compound:st067105|FD-Score:5.60|P-value:1.43E-8 ID:SGTC_1947|Compound:st076332|FD-Score:3.54|P-value:2.30E-4 ID:SGTC_2017|Compound:5132927|FD-Score:4.52|P-value:3.85E-6 ID:SGTC_2018|Compound:5133068|FD-Score:-3.80|P-value:6.83E-5 ID:SGTC_2413|Compound:inermin|FD-Score:-3.22|P-value:6.16E-4 ID:SGTC_2415|Compound:st077333|FD-Score:-5.90|P-value:1.78E-9 ID:SGTC_2426|Compound:5582046|FD-Score:-3.76|P-value:8.13E-5 ID:SGTC_2477|Compound:5967686|FD-Score:-3.80|P-value:6.82E-5 ID:SGTC_2488|Compound:5268135|FD-Score:4.04|P-value:3.11E-5 ID:SGTC_2525|Compound:harmine|FD-Score:-3.20|P-value:6.54E-4 ID:SGTC_2568|Compound:diosmetin|FD-Score:-3.14|P-value:7.94E-4 ID:SGTC_2574|Compound:2',4'-dihydroxychalcone|FD-Score:-3.73|P-value:9.13E-5 ID:SGTC_2666|Compound:tannic acid|FD-Score:3.84|P-value:7.23E-5 ID:SGTC_3051|Compound:9093778|FD-Score:3.60|P-value:1.81E-4 ID:SGTC_3085|Compound:9117376|FD-Score:-3.16|P-value:7.46E-4 ID:SGTC_3126|Compound:9127922|FD-Score:4.30|P-value:1.02E-5 ID:SGTC_3129|Compound:9127257|FD-Score:3.34|P-value:4.71E-4 ID:SGTC_3131|Compound:9127911|FD-Score:4.80|P-value:9.70E-7 ID:SGTC_3136|Compound:9095672|FD-Score:-3.51|P-value:2.14E-4 ID:SGTC_3265|Compound:9137266|FD-Score:-3.30|P-value:4.65E-4 ID:SGTC_3302|Compound:9125395|FD-Score:3.75|P-value:1.03E-4 ID:SGTC_3303|Compound:9125618|FD-Score:-4.40|P-value:5.02E-6 ID:SGTC_36|Compound:0335-0881|FD-Score:3.78|P-value:9.06E-5 ID:SGTC_96|Compound:0929-0093|FD-Score:3.27|P-value:6.22E-4 ID:SGTC_143|Compound:4032-1194|FD-Score:7.84|P-value:3.48E-15 ID:SGTC_144|Compound:4161-2749|FD-Score:8.55|P-value:1.02E-17 ID:SGTC_145|Compound:3737-0113|FD-Score:11.60|P-value:3.47E-31 ID:SGTC_146|Compound:3448-8650|FD-Score:7.26|P-value:2.74E-13 ID:SGTC_166|Compound:k072-0058|FD-Score:3.88|P-value:6.06E-5 ID:SGTC_184|Compound:1494-0393|FD-Score:3.57|P-value:2.04E-4 ID:SGTC_608|Compound:k048-0125|FD-Score:10.30|P-value:9.44E-25 ID:SGTC_669|Compound:0108-0021|FD-Score:3.37|P-value:4.36E-4 ID:SGTC_712|Compound:r015-0009|FD-Score:6.22|P-value:3.36E-10 ID:SGTC_813|Compound:k007-0158|FD-Score:3.62|P-value:1.72E-4 ID:SGTC_858|Compound:0438-0351|FD-Score:4.25|P-value:1.31E-5 ID:SGTC_900|Compound:1112-0045|FD-Score:-4.30|P-value:8.10E-6 ID:SGTC_1187|Compound:1391-0724|FD-Score:3.50|P-value:2.64E-4 ID:SGTC_1196|Compound:1588-0294|FD-Score:4.29|P-value:1.09E-5 ID:SGTC_1248|Compound:0469-0687|FD-Score:9.55|P-value:1.15E-21 ID:SGTC_1406|Compound:3909-7906|FD-Score:3.80|P-value:8.47E-5 ID:SGTC_1417|Compound:3966-0327|FD-Score:-3.29|P-value:4.73E-4 ID:SGTC_1432|Compound:4049-0298|FD-Score:3.54|P-value:2.32E-4 ID:SGTC_1434|Compound:4073-0095|FD-Score:4.01|P-value:3.52E-5 ID:SGTC_1915|Compound:st067105|FD-Score:5.60|P-value:1.43E-8 ID:SGTC_1947|Compound:st076332|FD-Score:3.54|P-value:2.30E-4 ID:SGTC_2017|Compound:5132927|FD-Score:4.52|P-value:3.85E-6 ID:SGTC_2018|Compound:5133068|FD-Score:-3.80|P-value:6.83E-5 ID:SGTC_2413|Compound:inermin|FD-Score:-3.22|P-value:6.16E-4 ID:SGTC_2415|Compound:st077333|FD-Score:-5.90|P-value:1.78E-9 ID:SGTC_2426|Compound:5582046|FD-Score:-3.76|P-value:8.13E-5 ID:SGTC_2477|Compound:5967686|FD-Score:-3.80|P-value:6.82E-5 ID:SGTC_2488|Compound:5268135|FD-Score:4.04|P-value:3.11E-5 ID:SGTC_2525|Compound:harmine|FD-Score:-3.20|P-value:6.54E-4 ID:SGTC_2568|Compound:diosmetin|FD-Score:-3.14|P-value:7.94E-4 ID:SGTC_2574|Compound:2',4'-dihydroxychalcone|FD-Score:-3.73|P-value:9.13E-5 ID:SGTC_2666|Compound:tannic acid|FD-Score:3.84|P-value:7.23E-5 ID:SGTC_3051|Compound:9093778|FD-Score:3.60|P-value:1.81E-4 ID:SGTC_3085|Compound:9117376|FD-Score:-3.16|P-value:7.46E-4 ID:SGTC_3126|Compound:9127922|FD-Score:4.30|P-value:1.02E-5 ID:SGTC_3129|Compound:9127257|FD-Score:3.34|P-value:4.71E-4 ID:SGTC_3131|Compound:9127911|FD-Score:4.80|P-value:9.70E-7 ID:SGTC_3136|Compound:9095672|FD-Score:-3.51|P-value:2.14E-4 ID:SGTC_3265|Compound:9137266|FD-Score:-3.30|P-value:4.65E-4 ID:SGTC_3302|Compound:9125395|FD-Score:3.75|P-value:1.03E-4 ID:SGTC_3303|Compound:9125618|FD-Score:-4.40|P-value:5.02E-6

Top fitness defect scores for YFL022C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_145 3737-0113 11.60 38.4 µM 3.47E-31 DNA intercalators 3 1.62E-31 0.92
2 SGTC_608 k048-0125 10.30 159.0 µM 9.44E-25 2 5.03E-25 6.12
3 SGTC_1248 0469-0687 9.55 58.6 µM 1.15E-21 2 6.57E-22 3.59
4 SGTC_144 4161-2749 8.55 112.4 µM 1.02E-17 DNA intercalators 2 6.34E-18 0.95
5 SGTC_143 4032-1194 7.84 15.7 µM 3.48E-15 1 2.29E-15 1.31
6 SGTC_146 3448-8650 7.26 53.1 µM 2.74E-13 NEO1-PIK1 3 1.89E-13 0.27
7 SGTC_712 r015-0009 6.22 80.6 µM 3.36E-10 3 2.49E-10 1.60
8 SGTC_1915 st067105 5.60 30.6 µM 1.43E-8 RNA pol III & RNase P/MRP 22 1.10E-8 0.06
9 SGTC_3131 9127911 4.80 49.5 µM 9.70E-7 5 7.84E-7 0.32
10 SGTC_2017 5132927 4.52 60.6 µM 3.85E-6 3 3.16E-6 0.24
11 SGTC_3126 9127922 4.30 49.5 µM 1.02E-5 fatty acid desaturase (OLE1) 27 8.45E-6 0.23
12 SGTC_1196 1588-0294 4.29 184.0 µM 1.09E-5 4 9.09E-6 0.19
13 SGTC_858 0438-0351 4.25 2.8 µM 1.31E-5 copper-dependent oxidative stress 4 1.09E-5 0.22
14 SGTC_2488 5268135 4.04 45.7 µM 3.11E-5 5 2.62E-5 0.04
15 SGTC_1434 4073-0095 4.01 163.0 µM 3.52E-5 2 2.98E-5 0.23
16 SGTC_166 k072-0058 3.88 166.4 µM 6.06E-5 DNA intercalators 7 5.15E-5 0.30
17 SGTC_2666 tannic acid 3.84 100.0 µM 7.23E-5 iron homeostasis 10 6.16E-5 0.01
18 SGTC_1406 3909-7906 3.80 11.9 µM 8.47E-5 60S ribosome export 6 7.23E-5 0.08
19 SGTC_36 0335-0881 3.78 14.2 µM 9.06E-5 ubiquinone biosynthesis & proteasome 35 7.74E-5 0.03
20 SGTC_3302 9125395 3.75 16.4 µM 1.03E-4 5 8.80E-5 0.30

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.342 1.27E-92 YLR060W FRS1 het Beta subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs2p to generate active enzyme; able to hydrolyze mis-aminoacylated tRNA-Phe, which could contribute to translational quality control
0.291 1.47E-66 YDR401W_d YDR401W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.269 8.12E-57 YPL183C RTT10 hom WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rrt2p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; evidence it interacts with Trm7p for 2'-O-methylation of N34 of substrate tRNAs; has a role in regulation of Ty1 transposition; human ortholog is WDR6
0.238 1.48E-44 YGL118C_d YGL118C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.231 1.04E-41 YNL124W NAF1 het RNA-binding protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Shq1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; similar to Gar1p
0.218 1.42E-37 YHL039W EFM1 hom Lysine methyltransferase involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.214 3.17E-36 YHR149C SKG6 hom Integral membrane protein that localizes primarily to growing sites such as the bud tip or the cell periphery; potential Cdc28p substrate; Skg6p interacts with Zds1p and Zds2p
0.206 1.33E-33 YMR168C CEP3 het Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain
0.197 1.23E-30 YOR077W RTS2 het Basic zinc-finger protein, similar to human and mouse Kin17 proteins which are chromatin-associated proteins involved in UV response and DNA replication
0.190 1.84E-28 YEL017C-A PMP2 hom Proteolipid associated with plasma membrane H(+)-ATPase (Pma1p); regulates plasma membrane H(+)-ATPase activity; protein abundance increases in response to DNA replication stress; PMP2 has a paralog, PMP1, that arose from the whole genome duplication
0.188 4.76E-28 YMR289W ABZ2 hom Aminodeoxychorismate lyase (4-amino-4-deoxychorismate lyase), catalyzes the third step in para-aminobenzoic acid biosynthesis; involved in folic acid biosynthesis
0.188 5.96E-28 YGL200C EMP24 hom Component of the p24 complex; binds to GPI anchor proteins and mediates their efficient transport from the ER to the Golgi; integral membrane protein that associates with endoplasmic reticulum-derived COPII-coated vesicles
0.187 8.56E-28 YHR020W YHR020W het Prolyl-tRNA synthetase; N-terminal domain shows weak homology to prokaryotic posttransfer editing domain, but does not possess posttransfer editing activity; may interact with ribosomes, based on co-purification experiments
0.185 3.78E-27 YNL264C PDR17 hom Phosphatidylinositol transfer protein (PITP), downregulates Plb1p-mediated turnover of phosphatidylcholine, found in the cytosol and microsomes, homologous to Pdr16p, deletion affects phospholipid composition
0.177 4.00E-25 YEL064C AVT2 hom Putative transporter, member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters