YFL027C / GYP8

GTPase-activating protein for yeast Rab family members; Ypt1p is the preferred in vitro substrate but also acts on Sec4p, Ypt31p and Ypt32p; involved in the regulation of ER to Golgi vesicle transport

Zygosity: Homozygous strain
fixedexpanded
Profile for YFL027C / GYP8

Click on Significant Values for Screen Details ID:SGTC_66|Compound:0109-0187|FD-Score:-3.32|P-value:3.97E-4 ID:SGTC_176|Compound:1486-1328|FD-Score:-3.14|P-value:7.73E-4 ID:SGTC_428|Compound:3448-4153|FD-Score:-3.14|P-value:7.72E-4 ID:SGTC_523|Compound:1934-0222|FD-Score:3.32|P-value:3.12E-4 ID:SGTC_532|Compound:1548-0774|FD-Score:-4.14|P-value:1.45E-5 ID:SGTC_540|Compound:1187-0821|FD-Score:-3.17|P-value:7.04E-4 ID:SGTC_543|Compound:3844-1095|FD-Score:-3.33|P-value:3.84E-4 ID:SGTC_750|Compound:4073-0104|FD-Score:-3.68|P-value:1.02E-4 ID:SGTC_934|Compound:2950-0684|FD-Score:4.02|P-value:1.81E-5 ID:SGTC_936|Compound:3005-4620|FD-Score:3.90|P-value:3.09E-5 ID:SGTC_1011|Compound:4112-3381|FD-Score:-3.29|P-value:4.43E-4 ID:SGTC_1082|Compound:camptothecin|FD-Score:-3.40|P-value:2.95E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-4.14|P-value:1.39E-5 ID:SGTC_1116|Compound:jasmonic acid|FD-Score:3.20|P-value:4.97E-4 ID:SGTC_1211|Compound:1056-0033|FD-Score:3.63|P-value:9.44E-5 ID:SGTC_1241|Compound:0364-0022|FD-Score:3.30|P-value:3.42E-4 ID:SGTC_1270|Compound:0831-1170|FD-Score:3.33|P-value:3.08E-4 ID:SGTC_1280|Compound:0880-0253|FD-Score:-3.53|P-value:1.85E-4 ID:SGTC_1967|Compound:st070177|FD-Score:3.25|P-value:4.11E-4 ID:SGTC_2088|Compound:5278280|FD-Score:-4.96|P-value:2.56E-7 ID:SGTC_2238|Compound:6671620|FD-Score:-3.86|P-value:4.87E-5 ID:SGTC_2241|Compound:6772625|FD-Score:-3.60|P-value:1.38E-4 ID:SGTC_2371|Compound:9071574|FD-Score:3.37|P-value:2.63E-4 ID:SGTC_2373|Compound:9071805|FD-Score:4.76|P-value:5.07E-7 ID:SGTC_2379|Compound:9035213|FD-Score:3.74|P-value:5.92E-5 ID:SGTC_2396|Compound:5342950|FD-Score:-3.29|P-value:4.57E-4 ID:SGTC_2535|Compound:4',4'-dimethoxydalbergione|FD-Score:4.28|P-value:5.52E-6 ID:SGTC_2614|Compound:arthonioic acid|FD-Score:-3.75|P-value:7.51E-5 ID:SGTC_2658|Compound:polymyxin b sulfate|FD-Score:3.32|P-value:3.16E-4 ID:SGTC_2691|Compound:st077356|FD-Score:3.68|P-value:7.84E-5 ID:SGTC_2801|Compound:7907800|FD-Score:3.12|P-value:6.63E-4 ID:SGTC_2834|Compound:9001755|FD-Score:-3.77|P-value:6.93E-5 ID:SGTC_2838|Compound:9002687|FD-Score:3.03|P-value:8.86E-4 ID:SGTC_3020|Compound:9089140|FD-Score:3.36|P-value:2.75E-4 ID:SGTC_3141|Compound:9094609|FD-Score:3.26|P-value:3.98E-4 ID:SGTC_3144|Compound:9087057|FD-Score:-4.30|P-value:6.96E-6 ID:SGTC_3259|Compound:9138485|FD-Score:3.89|P-value:3.19E-5 ID:SGTC_3282|Compound:9108498|FD-Score:3.66|P-value:8.31E-5 ID:SGTC_3346|Compound:9158254|FD-Score:-3.74|P-value:7.85E-5 ID:SGTC_66|Compound:0109-0187|FD-Score:-3.32|P-value:3.97E-4 ID:SGTC_176|Compound:1486-1328|FD-Score:-3.14|P-value:7.73E-4 ID:SGTC_428|Compound:3448-4153|FD-Score:-3.14|P-value:7.72E-4 ID:SGTC_523|Compound:1934-0222|FD-Score:3.32|P-value:3.12E-4 ID:SGTC_532|Compound:1548-0774|FD-Score:-4.14|P-value:1.45E-5 ID:SGTC_540|Compound:1187-0821|FD-Score:-3.17|P-value:7.04E-4 ID:SGTC_543|Compound:3844-1095|FD-Score:-3.33|P-value:3.84E-4 ID:SGTC_750|Compound:4073-0104|FD-Score:-3.68|P-value:1.02E-4 ID:SGTC_934|Compound:2950-0684|FD-Score:4.02|P-value:1.81E-5 ID:SGTC_936|Compound:3005-4620|FD-Score:3.90|P-value:3.09E-5 ID:SGTC_1011|Compound:4112-3381|FD-Score:-3.29|P-value:4.43E-4 ID:SGTC_1082|Compound:camptothecin|FD-Score:-3.40|P-value:2.95E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-4.14|P-value:1.39E-5 ID:SGTC_1116|Compound:jasmonic acid|FD-Score:3.20|P-value:4.97E-4 ID:SGTC_1211|Compound:1056-0033|FD-Score:3.63|P-value:9.44E-5 ID:SGTC_1241|Compound:0364-0022|FD-Score:3.30|P-value:3.42E-4 ID:SGTC_1270|Compound:0831-1170|FD-Score:3.33|P-value:3.08E-4 ID:SGTC_1280|Compound:0880-0253|FD-Score:-3.53|P-value:1.85E-4 ID:SGTC_1967|Compound:st070177|FD-Score:3.25|P-value:4.11E-4 ID:SGTC_2088|Compound:5278280|FD-Score:-4.96|P-value:2.56E-7 ID:SGTC_2238|Compound:6671620|FD-Score:-3.86|P-value:4.87E-5 ID:SGTC_2241|Compound:6772625|FD-Score:-3.60|P-value:1.38E-4 ID:SGTC_2371|Compound:9071574|FD-Score:3.37|P-value:2.63E-4 ID:SGTC_2373|Compound:9071805|FD-Score:4.76|P-value:5.07E-7 ID:SGTC_2379|Compound:9035213|FD-Score:3.74|P-value:5.92E-5 ID:SGTC_2396|Compound:5342950|FD-Score:-3.29|P-value:4.57E-4 ID:SGTC_2535|Compound:4',4'-dimethoxydalbergione|FD-Score:4.28|P-value:5.52E-6 ID:SGTC_2614|Compound:arthonioic acid|FD-Score:-3.75|P-value:7.51E-5 ID:SGTC_2658|Compound:polymyxin b sulfate|FD-Score:3.32|P-value:3.16E-4 ID:SGTC_2691|Compound:st077356|FD-Score:3.68|P-value:7.84E-5 ID:SGTC_2801|Compound:7907800|FD-Score:3.12|P-value:6.63E-4 ID:SGTC_2834|Compound:9001755|FD-Score:-3.77|P-value:6.93E-5 ID:SGTC_2838|Compound:9002687|FD-Score:3.03|P-value:8.86E-4 ID:SGTC_3020|Compound:9089140|FD-Score:3.36|P-value:2.75E-4 ID:SGTC_3141|Compound:9094609|FD-Score:3.26|P-value:3.98E-4 ID:SGTC_3144|Compound:9087057|FD-Score:-4.30|P-value:6.96E-6 ID:SGTC_3259|Compound:9138485|FD-Score:3.89|P-value:3.19E-5 ID:SGTC_3282|Compound:9108498|FD-Score:3.66|P-value:8.31E-5 ID:SGTC_3346|Compound:9158254|FD-Score:-3.74|P-value:7.85E-5

Top fitness defect scores for YFL027C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2373 9071805 4.76 200.0 µM 5.07E-7 4 9.49E-7
2 SGTC_2535 4',4'-dimethoxydalbergione 4.28 51.2 µM 5.52E-6 DNA damage response 12 9.34E-6
3 SGTC_934 2950-0684 4.02 338.0 µM 1.81E-5 22 2.91E-5
4 SGTC_936 3005-4620 3.90 401.0 µM 3.09E-5 19 4.86E-5
5 SGTC_3259 9138485 3.89 49.5 µM 3.19E-5 13 5.01E-5
6 SGTC_2379 9035213 3.74 200.0 µM 5.92E-5 27 9.04E-5
7 SGTC_2691 st077356 3.68 60.2 µM 7.84E-5 38 1.18E-4
8 SGTC_3282 9108498 3.66 15.5 µM 8.31E-5 35 1.25E-4
9 SGTC_1211 1056-0033 3.63 32.3 µM 9.44E-5 13 1.41E-4
10 SGTC_2371 9071574 3.37 200.0 µM 2.63E-4 28 3.77E-4
11 SGTC_3020 9089140 3.36 49.5 µM 2.75E-4 48 3.93E-4
12 SGTC_1270 0831-1170 3.33 17.3 µM 3.08E-4 26 4.39E-4
13 SGTC_523 1934-0222 3.32 13.7 µM 3.12E-4 50 4.44E-4
14 SGTC_2658 polymyxin b sulfate 3.32 77.0 µM 3.16E-4 NEO1 64 4.49E-4
15 SGTC_1241 0364-0022 3.30 50.8 µM 3.42E-4 ERG2 58 4.85E-4
16 SGTC_3141 9094609 3.26 49.5 µM 3.98E-4 iron homeostasis 63 5.60E-4
17 SGTC_1967 st070177 3.25 51.4 µM 4.11E-4 42 5.78E-4
18 SGTC_1116 jasmonic acid 3.20 1.6 µM 4.97E-4 46 6.93E-4
19 SGTC_2801 7907800 3.12 81.8 µM 6.63E-4 66 9.13E-4
20 SGTC_2838 9002687 3.03 71.4 µM 8.86E-4 fatty acid desaturase (OLE1) 62 0.00120

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.186 1.56E-27 YEL049W PAU2 hom Member of the seripauperin multigene family encoded mainly in subtelomeric regions, active during alcoholic fermentation, regulated by anaerobiosis, negatively regulated by oxygen, repressed by heme
0.177 4.69E-25 YKL131W_d YKL131W_d hom Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.176 6.98E-25 YGL146C_p RRT6_p hom Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; contains two putative transmembrane spans, but no significant homology to other known proteins
0.173 7.08E-24 YER071C_p TDA2_p hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; null mutant is sensitive to expression of the top1-T722A allele
0.170 3.93E-23 YDL110C TMA17 hom Protein of unknown function that associates with ribosomes; heterozygous deletion demonstrated increases in chromosome instability in a rad9 deletion background; protein abundance is decreased upon intracellular iron depletion
0.170 4.73E-23 YML052W SUR7 hom Plasma membrane protein that localizes to furrow-like invaginations (MCC patches); component of eisosomes; associated with endocytosis, along with Pil1p and Lsp1p; sporulation and plasma membrane sphingolipid content are altered in mutants
0.170 4.85E-23 YJR099W YUH1 hom Ubiquitin C-terminal hydrolase that cleaves ubiquitin-protein fusions to generate monomeric ubiquitin; hydrolyzes the peptide bond at the C-terminus of ubiquitin; also the major processing enzyme for the ubiquitin-like protein Rub1p
0.164 1.01E-21 YLR016C PML1 hom Subunit of the RES complex, which is required for nuclear retention of unspliced pre-mRNAs; acts in the same pathway as Pml39p and Mlp1p
0.164 1.41E-21 YOL060C MAM3 hom Protein required for normal mitochondrial morphology, has similarity to hemolysins
0.162 3.06E-21 YLR205C HMX1 hom ER localized heme oxygenase, involved in heme degradation during iron starvation and in the oxidative stress response; expression is regulated by AFT1 and oxidative stress; relocates to the perinuclear region in the presence of oxidants
0.148 7.27E-18 YCR102W-A_d YCR102W-A_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.148 7.79E-18 YER064C VHR2 hom Non-essential nuclear protein; null mutation has global effects on transcription; VHR2 has a paralog, VHR1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
0.144 4.20E-17 YNR028W CPR8 hom Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; similarity to Cpr4p suggests a potential role in the secretory pathway
0.143 8.01E-17 YMR147W_p YMR147W_p hom Putative protein of unknown function
0.143 8.97E-17 YOR300W_d YOR300W_d hom Dubious open reading frame, unlikely to encode a protein; overlaps with verified gene BUD7/YOR299W; mutation affects bipolar budding and bud site selection, though phenotype could be due to the mutation's effects on BUD7