HIPHOP chemogenomics database

YFL030W / AGX1

Alanine:glyoxylate aminotransferase (AGT), catalyzes the synthesis of glycine from glyoxylate, which is one of three pathways for glycine biosynthesis in yeast; has similarity to mammalian and plant alanine:glyoxylate aminotransferases

Zygosity: Homozygous strain

fixedexpanded
Profile for YFL030W / AGX1

Click on Significant Values for Screen Details

Top fitness defect scores for YFL030W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_2086	5274770	6.19	176.6 µM	2.28E-11		5	3.04E-10
2	SGTC_2005	4024620	6.06	188.0 µM	5.58E-11		1	6.70E-10
3	SGTC_1259	0671-0132	4.96	43.0 µM	6.75E-8		6	3.57E-7
4	SGTC_416	estradiol valerate	4.80	100.0 µM	1.65E-7		2	7.88E-7
5	SGTC_2002	4023503	4.61	131.0 µM	4.83E-7	mitochondrial processes	17	2.04E-6
6	SGTC_2795	7780010	4.24	54.5 µM	3.29E-6		22	1.12E-5
7	SGTC_2595	hexamethylquercetagetin	4.00	100.0 µM	1.06E-5		23	3.14E-5
8	SGTC_1127	asiatic acid	3.96	681.4 nM	1.28E-5		22	3.73E-5
9	SGTC_656	4090-1979	3.95	187.0 µM	1.38E-5		17	3.98E-5
10	SGTC_3019	9081971	3.94	49.5 µM	1.44E-5		17	4.13E-5
11	SGTC_1547	harmine	3.82	94.2 µM	2.42E-5	ergosterol biosynthesis	48	6.55E-5
12	SGTC_759	3486-0358	3.69	89.3 µM	4.41E-5		25	1.12E-4
13	SGTC_2781	5567276	3.51	71.4 µM	9.68E-5		74	2.25E-4
14	SGTC_1013	4112-3890	3.46	241.0 µM	1.19E-4	TSC3-RPN4	55	2.70E-4
15	SGTC_553	k007-0256	3.43	45.7 µM	1.37E-4	heme biosynthesis & mitochondrial translocase	39	3.05E-4
16	SGTC_6	0844-0013	3.42	19.8 µM	1.42E-4		148	3.17E-4
17	SGTC_2842	9000675	3.42	19.5 µM	1.44E-4		42	3.19E-4
18	SGTC_678	1218-2179	3.36	85.9 µM	1.78E-4	RNA pol III & RNase P/MRP	9	3.85E-4
19	SGTC_1034	k072-0165	3.33	12.7 µM	1.99E-4	Golgi	44	4.27E-4
20	SGTC_1248	0469-0687	3.33	58.6 µM	2.07E-4		54	4.41E-4

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.107	5.61E-10	YML003W_p	YML003W_p	hom	Putative protein of unknown function
0.107	6.07E-10	YOR155C	ISN1	hom	Inosine 5'-monophosphate (IMP)-specific 5'-nucleotidase, catalyzes the breakdown of IMP to inosine, does not show similarity to known 5'-nucleotidases from other organisms
0.105	1.18E-9	YJL106W	IME2	hom	Serine/threonine protein kinase involved in activation of meiosis, associates with Ime1p and mediates its stability, activates Ndt80p; IME2 expression is positively regulated by Ime1p
0.096	2.75E-8	YDL028C	MPS1	het	Dual-specificity kinase; required for spindle pole body (SPB) duplication and spindle checkpoint function; substrates include SPB proteins Spc42p, Spc110p, and Spc98p, mitotic exit network protein Mob1p, kinetochore protein Cnn1p, and checkpoint protein Mad1p
0.089	2.18E-7	YOR227W	HER1	hom	Protein of unknown function required for proliferation or remodeling of the ER that is caused by overexpression of Hmg2p; may interact with ribosomes, based on co-purification experiments; HER1 has a paralog, GIP3, that arose from the whole genome duplication
0.089	2.71E-7	YJR079W_p	YJR079W_p	hom	Putative protein of unknown function; mutation results in impaired mitochondrial respiration
0.089	2.68E-7	YDR312W	SSF2	hom	Protein required for ribosomal large subunit maturation; functionally redundant with Ssf1p; member of the Brix family; SSF2 has a paralog, SSF1, that arose from the whole genome duplication
0.087	4.99E-7	YGL210W	YPT32	hom	Rab family GTPase involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; protein abundance increases in response to DNA replication stress; YPT32 has a paralog, YPT31, that arose from the whole genome duplication
0.085	8.72E-7	YER067C-A_d	YER067C-A_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YER067W
0.084	1.13E-6	YNL218W	MGS1	hom	Protein with DNA-dependent ATPase and ssDNA annealing activities; involved in maintenance of genome; interacts functionally with DNA polymerase delta; homolog of human Werner helicase interacting protein (WHIP); forms nuclear foci upon DNA replication stress
0.080	3.75E-6	YAR031W	PRM9	hom	Pheromone-regulated protein with 3 predicted transmembrane segments and an FF sequence, a motif involved in COPII binding; member of DUP240 gene family
0.076	1.04E-5	YBR004C	GPI18	het	Functional ortholog of human PIG-V, which is a mannosyltransferase that transfers the second mannose in glycosylphosphatidylinositol biosynthesis; the authentic, non-tagged protein was localized to mitochondria
0.076	1.08E-5	YLR177W_p	YLR177W_p	hom	Putative protein of unknown function; phosphorylated by Dbf2p-Mob1p in vitro; some strains contain microsatellite polymophisms at this locus; not an essential gene; YLR177W has a paralog, PSP1, that arose from the whole genome duplication
0.076	1.11E-5	YDL022W	GPD1	hom	NAD-dependent glycerol-3-phosphate dehydrogenase; key enzyme of glycerol synthesis, essential for growth under osmotic stress; expression regulated by high-osmolarity glycerol response pathway; homolog of Gpd2p; protein abundance increases in response to DNA replication stress; constitutively inactivated via phosphorylation by the protein kinases YPK1 and YPK2, dephosphorylation increases catalytic activity
0.075	1.40E-5	YGR009C	SEC9	het	t-SNARE protein important for fusion of secretory vesicles with the plasma membrane; similar to but not functionally redundant with Spo20p; SNAP-25 homolog