YFL031W / HAC1

Basic leucine zipper (bZIP) transcription factor (ATF/CREB1 homolog); regulates the unfolded protein response, via UPRE binding, and membrane biogenesis; ER stress-induced splicing pathway facilitates efficient Hac1p synthesis; protein abundance increases in response to DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YFL031W / HAC1

Click on Significant Values for Screen Details ID:SGTC_16|Compound:erodoxin|FD-Score:33.00|P-value:3.13E-259 ID:SGTC_22|Compound:0964-0034|FD-Score:33.80|P-value:2.02E-270 ID:SGTC_58|Compound:0957-0393|FD-Score:-3.02|P-value:9.49E-4 ID:SGTC_67|Compound:0335-0847|FD-Score:5.40|P-value:7.93E-9 ID:SGTC_86|Compound:0417-1630|FD-Score:7.13|P-value:4.73E-14 ID:SGTC_107|Compound:0358-0022|FD-Score:4.46|P-value:1.50E-6 ID:SGTC_108|Compound:0509-0257|FD-Score:17.70|P-value:2.39E-76 ID:SGTC_112|Compound:phenothiazine|FD-Score:7.10|P-value:6.10E-14 ID:SGTC_132|Compound:0417-1715|FD-Score:-3.75|P-value:5.77E-5 ID:SGTC_137|Compound:0438-0332|FD-Score:8.19|P-value:6.06E-18 ID:SGTC_142|Compound:0335-0849|FD-Score:3.49|P-value:1.26E-4 ID:SGTC_143|Compound:4032-1194|FD-Score:7.36|P-value:7.60E-15 ID:SGTC_160|Compound:3453-0671|FD-Score:-3.08|P-value:7.80E-4 ID:SGTC_165|Compound:k038-0023|FD-Score:4.27|P-value:3.92E-6 ID:SGTC_196|Compound:k015-0023|FD-Score:3.10|P-value:5.60E-4 ID:SGTC_255|Compound:tunicamycin|FD-Score:24.30|P-value:4.40E-141 ID:SGTC_353|Compound:1988-1175|FD-Score:23.90|P-value:1.17E-136 ID:SGTC_367|Compound:0335-0881|FD-Score:3.24|P-value:3.36E-4 ID:SGTC_380|Compound:3970-0968|FD-Score:5.02|P-value:7.48E-8 ID:SGTC_406|Compound:estrone|FD-Score:3.09|P-value:5.76E-4 ID:SGTC_410|Compound:α-linolenic acid|FD-Score:3.95|P-value:1.76E-5 ID:SGTC_418|Compound:lividomycin a|FD-Score:3.53|P-value:1.03E-4 ID:SGTC_433|Compound:pimozide|FD-Score:-3.42|P-value:2.19E-4 ID:SGTC_513|Compound:4245-1575|FD-Score:12.20|P-value:2.90E-37 ID:SGTC_531|Compound:3788-1495|FD-Score:19.60|P-value:3.09E-93 ID:SGTC_588|Compound:3970-1144|FD-Score:3.26|P-value:3.02E-4 ID:SGTC_592|Compound:k241-0073|FD-Score:3.06|P-value:6.54E-4 ID:SGTC_780|Compound:0109-0199|FD-Score:3.44|P-value:1.50E-4 ID:SGTC_842|Compound:0271-0008|FD-Score:3.01|P-value:7.85E-4 ID:SGTC_852|Compound:0418-0143|FD-Score:3.72|P-value:4.75E-5 ID:SGTC_902|Compound:1120-0002|FD-Score:3.36|P-value:2.07E-4 ID:SGTC_983|Compound:1491-0821|FD-Score:-3.05|P-value:8.62E-4 ID:SGTC_989|Compound:1501-0895|FD-Score:-3.07|P-value:8.03E-4 ID:SGTC_1121|Compound:fusaric acid|FD-Score:-3.39|P-value:2.43E-4 ID:SGTC_1124|Compound:fisetin|FD-Score:3.03|P-value:7.32E-4 ID:SGTC_1133|Compound:3473-0247|FD-Score:4.01|P-value:1.28E-5 ID:SGTC_1239|Compound:0337-0584|FD-Score:23.70|P-value:1.09E-134 ID:SGTC_1253|Compound:0550-0034|FD-Score:-3.41|P-value:2.24E-4 ID:SGTC_1256|Compound:0570-0116|FD-Score:4.23|P-value:4.61E-6 ID:SGTC_1260|Compound:0700-1617|FD-Score:-3.30|P-value:3.45E-4 ID:SGTC_1262|Compound:0737-0114|FD-Score:-4.21|P-value:7.10E-6 ID:SGTC_1264|Compound:0783-0040|FD-Score:-4.85|P-value:2.83E-7 ID:SGTC_1321|Compound:1326-2144|FD-Score:3.12|P-value:5.13E-4 ID:SGTC_1323|Compound:1348-1351|FD-Score:3.57|P-value:8.87E-5 ID:SGTC_1337|Compound:1486-1011|FD-Score:3.01|P-value:7.82E-4 ID:SGTC_1351|Compound:1496-0003|FD-Score:3.20|P-value:3.82E-4 ID:SGTC_1452|Compound:4194-0072|FD-Score:-4.06|P-value:1.45E-5 ID:SGTC_1462|Compound:k081-0014|FD-Score:3.74|P-value:4.37E-5 ID:SGTC_1637|Compound:st007924|FD-Score:3.40|P-value:1.75E-4 ID:SGTC_1683|Compound:st010134|FD-Score:3.12|P-value:5.27E-4 ID:SGTC_1713|Compound:st033146|FD-Score:3.38|P-value:1.90E-4 ID:SGTC_1718|Compound:roemerine|FD-Score:3.47|P-value:1.33E-4 ID:SGTC_1724|Compound:st037281|FD-Score:3.08|P-value:6.10E-4 ID:SGTC_1789|Compound:tunicamycin|FD-Score:9.44|P-value:3.46E-23 ID:SGTC_1872|Compound:amphotericin b|FD-Score:3.05|P-value:6.79E-4 ID:SGTC_1887|Compound:st057648|FD-Score:4.26|P-value:3.98E-6 ID:SGTC_1889|Compound:st059080|FD-Score:-4.15|P-value:9.50E-6 ID:SGTC_1895|Compound:st058434|FD-Score:5.07|P-value:5.73E-8 ID:SGTC_1962|Compound:st076595|FD-Score:3.43|P-value:1.56E-4 ID:SGTC_1992|Compound:st073643|FD-Score:-3.37|P-value:2.65E-4 ID:SGTC_2413|Compound:inermin|FD-Score:3.63|P-value:7.00E-5 ID:SGTC_2436|Compound:5633522|FD-Score:-3.20|P-value:5.00E-4 ID:SGTC_2450|Compound:5739737|FD-Score:2.95|P-value:9.73E-4 ID:SGTC_2637|Compound:3-alpha-acetoxydihydrodeoxygedunin|FD-Score:3.55|P-value:9.75E-5 ID:SGTC_2651|Compound:1327-0036|FD-Score:9.45|P-value:3.19E-23 ID:SGTC_2665|Compound:lovastatin|FD-Score:4.72|P-value:3.86E-7 ID:SGTC_2694|Compound:st077685|FD-Score:-3.43|P-value:2.11E-4 ID:SGTC_2757|Compound:clomiphene|FD-Score:-3.14|P-value:6.39E-4 ID:SGTC_2781|Compound:5567276|FD-Score:-3.73|P-value:6.21E-5 ID:SGTC_2804|Compound:7954240|FD-Score:-3.05|P-value:8.81E-4 ID:SGTC_2851|Compound:9015922|FD-Score:3.19|P-value:4.03E-4 ID:SGTC_2929|Compound:9013348|FD-Score:3.39|P-value:1.82E-4 ID:SGTC_2940|Compound:9060638|FD-Score:-3.33|P-value:3.10E-4 ID:SGTC_2978|Compound:9011784|FD-Score:-3.49|P-value:1.67E-4 ID:SGTC_2997|Compound:9067075|FD-Score:14.40|P-value:6.41E-51 ID:SGTC_3059|Compound:1327-0034|FD-Score:9.48|P-value:2.43E-23 ID:SGTC_3060|Compound:1327-0027|FD-Score:9.03|P-value:2.21E-21 ID:SGTC_3063|Compound:1327-0034|FD-Score:10.50|P-value:4.58E-28 ID:SGTC_3078|Compound:9116413|FD-Score:11.00|P-value:1.74E-30 ID:SGTC_3137|Compound:9097352|FD-Score:3.66|P-value:6.12E-5 ID:SGTC_3138|Compound:9097216|FD-Score:5.60|P-value:2.39E-9 ID:SGTC_3142|Compound:9086498|FD-Score:4.36|P-value:2.45E-6 ID:SGTC_3153|Compound:9100258|FD-Score:3.11|P-value:5.30E-4 ID:SGTC_3166|Compound:9102297|FD-Score:4.23|P-value:4.65E-6 ID:SGTC_3199|Compound:9111318|FD-Score:2.98|P-value:8.56E-4 ID:SGTC_3229|Compound:9132204|FD-Score:3.57|P-value:8.80E-5 ID:SGTC_3331|Compound:9141060|FD-Score:-3.34|P-value:2.98E-4 ID:SGTC_16|Compound:erodoxin|FD-Score:33.00|P-value:3.13E-259 ID:SGTC_22|Compound:0964-0034|FD-Score:33.80|P-value:2.02E-270 ID:SGTC_58|Compound:0957-0393|FD-Score:-3.02|P-value:9.49E-4 ID:SGTC_67|Compound:0335-0847|FD-Score:5.40|P-value:7.93E-9 ID:SGTC_86|Compound:0417-1630|FD-Score:7.13|P-value:4.73E-14 ID:SGTC_107|Compound:0358-0022|FD-Score:4.46|P-value:1.50E-6 ID:SGTC_108|Compound:0509-0257|FD-Score:17.70|P-value:2.39E-76 ID:SGTC_112|Compound:phenothiazine|FD-Score:7.10|P-value:6.10E-14 ID:SGTC_132|Compound:0417-1715|FD-Score:-3.75|P-value:5.77E-5 ID:SGTC_137|Compound:0438-0332|FD-Score:8.19|P-value:6.06E-18 ID:SGTC_142|Compound:0335-0849|FD-Score:3.49|P-value:1.26E-4 ID:SGTC_143|Compound:4032-1194|FD-Score:7.36|P-value:7.60E-15 ID:SGTC_160|Compound:3453-0671|FD-Score:-3.08|P-value:7.80E-4 ID:SGTC_165|Compound:k038-0023|FD-Score:4.27|P-value:3.92E-6 ID:SGTC_196|Compound:k015-0023|FD-Score:3.10|P-value:5.60E-4 ID:SGTC_255|Compound:tunicamycin|FD-Score:24.30|P-value:4.40E-141 ID:SGTC_353|Compound:1988-1175|FD-Score:23.90|P-value:1.17E-136 ID:SGTC_367|Compound:0335-0881|FD-Score:3.24|P-value:3.36E-4 ID:SGTC_380|Compound:3970-0968|FD-Score:5.02|P-value:7.48E-8 ID:SGTC_406|Compound:estrone|FD-Score:3.09|P-value:5.76E-4 ID:SGTC_410|Compound:α-linolenic acid|FD-Score:3.95|P-value:1.76E-5 ID:SGTC_418|Compound:lividomycin a|FD-Score:3.53|P-value:1.03E-4 ID:SGTC_433|Compound:pimozide|FD-Score:-3.42|P-value:2.19E-4 ID:SGTC_513|Compound:4245-1575|FD-Score:12.20|P-value:2.90E-37 ID:SGTC_531|Compound:3788-1495|FD-Score:19.60|P-value:3.09E-93 ID:SGTC_588|Compound:3970-1144|FD-Score:3.26|P-value:3.02E-4 ID:SGTC_592|Compound:k241-0073|FD-Score:3.06|P-value:6.54E-4 ID:SGTC_780|Compound:0109-0199|FD-Score:3.44|P-value:1.50E-4 ID:SGTC_842|Compound:0271-0008|FD-Score:3.01|P-value:7.85E-4 ID:SGTC_852|Compound:0418-0143|FD-Score:3.72|P-value:4.75E-5 ID:SGTC_902|Compound:1120-0002|FD-Score:3.36|P-value:2.07E-4 ID:SGTC_983|Compound:1491-0821|FD-Score:-3.05|P-value:8.62E-4 ID:SGTC_989|Compound:1501-0895|FD-Score:-3.07|P-value:8.03E-4 ID:SGTC_1121|Compound:fusaric acid|FD-Score:-3.39|P-value:2.43E-4 ID:SGTC_1124|Compound:fisetin|FD-Score:3.03|P-value:7.32E-4 ID:SGTC_1133|Compound:3473-0247|FD-Score:4.01|P-value:1.28E-5 ID:SGTC_1239|Compound:0337-0584|FD-Score:23.70|P-value:1.09E-134 ID:SGTC_1253|Compound:0550-0034|FD-Score:-3.41|P-value:2.24E-4 ID:SGTC_1256|Compound:0570-0116|FD-Score:4.23|P-value:4.61E-6 ID:SGTC_1260|Compound:0700-1617|FD-Score:-3.30|P-value:3.45E-4 ID:SGTC_1262|Compound:0737-0114|FD-Score:-4.21|P-value:7.10E-6 ID:SGTC_1264|Compound:0783-0040|FD-Score:-4.85|P-value:2.83E-7 ID:SGTC_1321|Compound:1326-2144|FD-Score:3.12|P-value:5.13E-4 ID:SGTC_1323|Compound:1348-1351|FD-Score:3.57|P-value:8.87E-5 ID:SGTC_1337|Compound:1486-1011|FD-Score:3.01|P-value:7.82E-4 ID:SGTC_1351|Compound:1496-0003|FD-Score:3.20|P-value:3.82E-4 ID:SGTC_1452|Compound:4194-0072|FD-Score:-4.06|P-value:1.45E-5 ID:SGTC_1462|Compound:k081-0014|FD-Score:3.74|P-value:4.37E-5 ID:SGTC_1637|Compound:st007924|FD-Score:3.40|P-value:1.75E-4 ID:SGTC_1683|Compound:st010134|FD-Score:3.12|P-value:5.27E-4 ID:SGTC_1713|Compound:st033146|FD-Score:3.38|P-value:1.90E-4 ID:SGTC_1718|Compound:roemerine|FD-Score:3.47|P-value:1.33E-4 ID:SGTC_1724|Compound:st037281|FD-Score:3.08|P-value:6.10E-4 ID:SGTC_1789|Compound:tunicamycin|FD-Score:9.44|P-value:3.46E-23 ID:SGTC_1872|Compound:amphotericin b|FD-Score:3.05|P-value:6.79E-4 ID:SGTC_1887|Compound:st057648|FD-Score:4.26|P-value:3.98E-6 ID:SGTC_1889|Compound:st059080|FD-Score:-4.15|P-value:9.50E-6 ID:SGTC_1895|Compound:st058434|FD-Score:5.07|P-value:5.73E-8 ID:SGTC_1962|Compound:st076595|FD-Score:3.43|P-value:1.56E-4 ID:SGTC_1992|Compound:st073643|FD-Score:-3.37|P-value:2.65E-4 ID:SGTC_2413|Compound:inermin|FD-Score:3.63|P-value:7.00E-5 ID:SGTC_2436|Compound:5633522|FD-Score:-3.20|P-value:5.00E-4 ID:SGTC_2450|Compound:5739737|FD-Score:2.95|P-value:9.73E-4 ID:SGTC_2637|Compound:3-alpha-acetoxydihydrodeoxygedunin|FD-Score:3.55|P-value:9.75E-5 ID:SGTC_2651|Compound:1327-0036|FD-Score:9.45|P-value:3.19E-23 ID:SGTC_2665|Compound:lovastatin|FD-Score:4.72|P-value:3.86E-7 ID:SGTC_2694|Compound:st077685|FD-Score:-3.43|P-value:2.11E-4 ID:SGTC_2757|Compound:clomiphene|FD-Score:-3.14|P-value:6.39E-4 ID:SGTC_2781|Compound:5567276|FD-Score:-3.73|P-value:6.21E-5 ID:SGTC_2804|Compound:7954240|FD-Score:-3.05|P-value:8.81E-4 ID:SGTC_2851|Compound:9015922|FD-Score:3.19|P-value:4.03E-4 ID:SGTC_2929|Compound:9013348|FD-Score:3.39|P-value:1.82E-4 ID:SGTC_2940|Compound:9060638|FD-Score:-3.33|P-value:3.10E-4 ID:SGTC_2978|Compound:9011784|FD-Score:-3.49|P-value:1.67E-4 ID:SGTC_2997|Compound:9067075|FD-Score:14.40|P-value:6.41E-51 ID:SGTC_3059|Compound:1327-0034|FD-Score:9.48|P-value:2.43E-23 ID:SGTC_3060|Compound:1327-0027|FD-Score:9.03|P-value:2.21E-21 ID:SGTC_3063|Compound:1327-0034|FD-Score:10.50|P-value:4.58E-28 ID:SGTC_3078|Compound:9116413|FD-Score:11.00|P-value:1.74E-30 ID:SGTC_3137|Compound:9097352|FD-Score:3.66|P-value:6.12E-5 ID:SGTC_3138|Compound:9097216|FD-Score:5.60|P-value:2.39E-9 ID:SGTC_3142|Compound:9086498|FD-Score:4.36|P-value:2.45E-6 ID:SGTC_3153|Compound:9100258|FD-Score:3.11|P-value:5.30E-4 ID:SGTC_3166|Compound:9102297|FD-Score:4.23|P-value:4.65E-6 ID:SGTC_3199|Compound:9111318|FD-Score:2.98|P-value:8.56E-4 ID:SGTC_3229|Compound:9132204|FD-Score:3.57|P-value:8.80E-5 ID:SGTC_3331|Compound:9141060|FD-Score:-3.34|P-value:2.98E-4

Top fitness defect scores for YFL031W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_22 0964-0034 33.80 14.5 µM 2.02E-270 unfolded protein response 1 4.32E-250
2 SGTC_16 erodoxin 33.00 22.8 µM 3.13E-259 unfolded protein response 2 9.73E-240
3 SGTC_255 tunicamycin 24.30 200.5 nM 4.40E-141 unfolded protein response 3 1.90E-130
4 SGTC_353 1988-1175 23.90 58.3 µM 1.17E-136 unfolded protein response 1 2.34E-126
5 SGTC_1239 0337-0584 23.70 35.4 µM 1.09E-134 unfolded protein response 4 1.55E-124
6 SGTC_531 3788-1495 19.60 174.0 µM 3.09E-93 unfolded protein response 3 3.34E-86
7 SGTC_108 0509-0257 17.70 621.1 µM 2.39E-76 1 1.37E-70
8 SGTC_2997 9067075 14.40 71.4 µM 6.41E-51 unfolded protein response 3 4.38E-47
9 SGTC_513 4245-1575 12.20 41.7 µM 2.90E-37 unfolded protein response 9 1.81E-34
10 SGTC_3078 9116413 11.00 49.5 µM 1.74E-30 unfolded protein response 3 3.32E-28
11 SGTC_3063 1327-0034 10.50 4.0 µM 4.58E-28 6 5.70E-26
12 SGTC_3059 1327-0034 9.48 3.0 µM 2.43E-23 12 1.32E-21
13 SGTC_2651 1327-0036 9.45 5.0 µM 3.19E-23 29 1.69E-21
14 SGTC_1789 tunicamycin 9.44 25.0 nM 3.46E-23 unfolded protein response 7 1.83E-21
15 SGTC_3060 1327-0027 9.03 12.5 µM 2.21E-21 8 8.49E-20
16 SGTC_137 0438-0332 8.19 85.4 µM 6.06E-18 ubiquinone biosynthesis & proteasome 35 1.26E-16
17 SGTC_143 4032-1194 7.36 15.7 µM 7.60E-15 11 9.16E-14
18 SGTC_86 0417-1630 7.13 471.9 µM 4.73E-14 ubiquinone biosynthesis & proteasome 70 4.95E-13
19 SGTC_112 phenothiazine 7.10 287.2 µM 6.10E-14 unfolded protein response 2 6.26E-13
20 SGTC_3138 9097216 5.60 49.5 µM 2.39E-9 1 1.08E-8

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.759 0 YHR079C IRE1 hom Serine-threonine kinase and endoribonuclease; transmembrane protein that mediates the unfolded protein response (UPR) by regulating Hac1p synthesis through HAC1 mRNA splicing; Kar2p binds inactive Ire1p and releases from it upon ER stress
0.731 0 YFL032W_d YFL032W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene HAC1/YFL031W; YFL032W is not an essential gene
0.467 3.13E-181 YML130C ERO1 het Thiol oxidase required for oxidative protein folding in the ER; essential for maintaining proper redox balance in ER; feedback regulation of Ero1p occurs via reduction and oxidation of Ero1p regulatory bonds; reduced Pdi1p activates Ero1p by direct reduction of Ero1p regulatory bonds; depletion of thiol substrates and accumulation of oxidized Pdi1p results in inactivation of Ero1p by both Pdi1p-mediated oxidation and autonomous oxidation of Ero1p regulatory bonds
0.324 1.16E-82 YDL045C FAD1 het Flavin adenine dinucleotide (FAD) synthetase, performs the second step in synthesis of FAD from riboflavin
0.301 4.68E-71 YPL244C HUT1 hom Protein with a role in UDP-galactose transport to the Golgi lumen, has similarity to human UDP-galactose transporter UGTrel1, exhibits a genetic interaction with S. cerevisiae ERO1
0.283 1.04E-62 YMR161W HLJ1 hom Co-chaperone for Hsp40p, anchored in the ER membrane; with its homolog Ydj1p promotes ER-associated protein degradation (ERAD) of integral membrane substrates; similar to E. coli DnaJ
0.270 5.28E-57 YBR243C ALG7 het UDP-N-acetyl-glucosamine-1-P transferase, transfers Glc-Nac-P from UDP-GlcNac to Dol-P in the ER in the first step of the dolichol pathway of protein asparagine-linked glycosylation; inhibited by tunicamycin
0.254 1.47E-50 YLR292C SEC72 hom Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER
0.224 1.65E-39 YBR260C RGD1 hom GTPase-activating protein (RhoGAP) for Rho3p and Rho4p, possibly involved in control of actin cytoskeleton organization
0.213 7.52E-36 YGL093W SPC105 het Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components
0.213 1.06E-35 YPR107C YTH1 het Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation
0.204 9.50E-33 YGR196C FYV8 hom Protein of unknown function, required for survival upon exposure to K1 killer toxin
0.200 9.67E-32 YNL287W SEC21 het Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo
0.184 4.76E-27 YDL096C_d OPI6_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene PMT1/YDL095W; YDL096C is not essential
0.169 6.04E-23 YMR215W GAS3 hom Low abundance, possibly inactive member of the GAS family of GPI-containing proteins; putative 1,3-beta-glucanosyltransferase with similarity to other GAS family members; localizes to the cell wall; mRNA induced during sporulation