YFL036W / RPO41

Mitochondrial RNA polymerase; single subunit enzyme similar to those of T3 and T7 bacteriophages; requires a specificity subunit encoded by MTF1 for promoter recognition; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation

Zygosity: Homozygous strain
fixedexpanded
Profile for YFL036W / RPO41

Click on Significant Values for Screen Details ID:SGTC_73|Compound:0327-0325|FD-Score:3.54|P-value:2.34E-4 ID:SGTC_116|Compound:0155-0182|FD-Score:7.43|P-value:9.24E-14 ID:SGTC_119|Compound:0121-0043|FD-Score:3.41|P-value:3.68E-4 ID:SGTC_141|Compound:0443-0274|FD-Score:4.64|P-value:2.19E-6 ID:SGTC_267|Compound:4-Nitroquinoline-1-oxide  (4-NQO)|FD-Score:3.49|P-value:2.78E-4 ID:SGTC_275|Compound:trichlorophene|FD-Score:28.90|P-value:6.38E-181 ID:SGTC_310|Compound:1273-0058|FD-Score:8.00|P-value:1.12E-15 ID:SGTC_325|Compound:2810-4341|FD-Score:4.33|P-value:9.04E-6 ID:SGTC_341|Compound:1486-1430|FD-Score:4.37|P-value:7.43E-6 ID:SGTC_350|Compound:k064-0020|FD-Score:8.80|P-value:1.41E-18 ID:SGTC_365|Compound:0121-0052|FD-Score:20.10|P-value:9.74E-89 ID:SGTC_372|Compound:0155-0182|FD-Score:6.47|P-value:7.21E-11 ID:SGTC_383|Compound:2324-0395|FD-Score:12.80|P-value:3.21E-37 ID:SGTC_397|Compound:arbidol|FD-Score:3.20|P-value:7.80E-4 ID:SGTC_403|Compound:arphamenine a|FD-Score:-3.56|P-value:1.97E-4 ID:SGTC_431|Compound:k089-0097|FD-Score:-3.49|P-value:2.59E-4 ID:SGTC_440|Compound:fluspirilene|FD-Score:-4.23|P-value:1.27E-5 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-5.15|P-value:1.54E-7 ID:SGTC_490|Compound:phenamil|FD-Score:3.48|P-value:2.86E-4 ID:SGTC_547|Compound:1120-0019|FD-Score:7.82|P-value:4.75E-15 ID:SGTC_607|Compound:4491-0986|FD-Score:4.52|P-value:3.87E-6 ID:SGTC_617|Compound:3770-0098|FD-Score:-3.64|P-value:1.44E-4 ID:SGTC_620|Compound:0109-0052|FD-Score:4.22|P-value:1.50E-5 ID:SGTC_636|Compound:0kpi-0074|FD-Score:3.18|P-value:8.37E-4 ID:SGTC_639|Compound:0833-0042|FD-Score:-4.45|P-value:4.75E-6 ID:SGTC_647|Compound:0140-0236|FD-Score:4.74|P-value:1.35E-6 ID:SGTC_778|Compound:3456-2434|FD-Score:6.18|P-value:4.62E-10 ID:SGTC_847|Compound:0342-0274|FD-Score:4.56|P-value:3.16E-6 ID:SGTC_848|Compound:0368-0089|FD-Score:3.37|P-value:4.35E-4 ID:SGTC_851|Compound:0394-0702|FD-Score:3.94|P-value:4.90E-5 ID:SGTC_902|Compound:1120-0002|FD-Score:3.16|P-value:8.78E-4 ID:SGTC_908|Compound:3448-7491|FD-Score:10.70|P-value:1.19E-26 ID:SGTC_909|Compound:3448-9333|FD-Score:3.37|P-value:4.26E-4 ID:SGTC_1036|Compound:k216-0215|FD-Score:3.52|P-value:2.45E-4 ID:SGTC_1057|Compound:3448-5381|FD-Score:3.59|P-value:1.90E-4 ID:SGTC_1123|Compound:mangostin|FD-Score:6.29|P-value:2.36E-10 ID:SGTC_1147|Compound:3809-1045|FD-Score:3.17|P-value:8.65E-4 ID:SGTC_1154|Compound:4088-0362|FD-Score:5.87|P-value:3.03E-9 ID:SGTC_1162|Compound:k048-0130|FD-Score:-3.34|P-value:4.35E-4 ID:SGTC_1240|Compound:0356-0016|FD-Score:25.20|P-value:3.07E-138 ID:SGTC_1248|Compound:0469-0687|FD-Score:-3.33|P-value:4.54E-4 ID:SGTC_1347|Compound:1493-0251|FD-Score:11.10|P-value:2.50E-28 ID:SGTC_1353|Compound:1498-1206|FD-Score:4.13|P-value:2.20E-5 ID:SGTC_1683|Compound:st010134|FD-Score:-3.63|P-value:1.50E-4 ID:SGTC_1787|Compound:fluconazole|FD-Score:-3.72|P-value:1.06E-4 ID:SGTC_1862|Compound:st057513|FD-Score:-3.67|P-value:1.29E-4 ID:SGTC_1982|Compound:st072467|FD-Score:-3.85|P-value:6.39E-5 ID:SGTC_1988|Compound:st071872|FD-Score:3.36|P-value:4.39E-4 ID:SGTC_1998|Compound:st075797|FD-Score:9.10|P-value:9.20E-20 ID:SGTC_2037|Compound:5169046|FD-Score:3.19|P-value:8.02E-4 ID:SGTC_2098|Compound:5376439|FD-Score:3.77|P-value:9.45E-5 ID:SGTC_2408|Compound:sulbentine|FD-Score:3.87|P-value:6.37E-5 ID:SGTC_2476|Compound:5806916|FD-Score:-3.64|P-value:1.48E-4 ID:SGTC_2504|Compound:purpurogallin|FD-Score:-3.60|P-value:1.66E-4 ID:SGTC_2505|Compound:galangin|FD-Score:-3.78|P-value:8.41E-5 ID:SGTC_2542|Compound:2-hydroxy-5 (6)epoxy-tetrahydrocaryophyllene|FD-Score:4.17|P-value:1.83E-5 ID:SGTC_2613|Compound:3',4'-dimethoxydalbergione|FD-Score:3.41|P-value:3.66E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:5.74|P-value:6.48E-9 ID:SGTC_2829|Compound:7992743|FD-Score:-3.14|P-value:8.89E-4 ID:SGTC_2833|Compound:9001315|FD-Score:4.87|P-value:7.04E-7 ID:SGTC_2911|Compound:7942208|FD-Score:3.69|P-value:1.28E-4 ID:SGTC_2925|Compound:9010536|FD-Score:6.19|P-value:4.28E-10 ID:SGTC_2930|Compound:9013230|FD-Score:4.30|P-value:1.02E-5 ID:SGTC_2933|Compound:9026794|FD-Score:-3.99|P-value:3.57E-5 ID:SGTC_2965|Compound:9082772|FD-Score:10.40|P-value:2.86E-25 ID:SGTC_3062|Compound:9077494|FD-Score:6.63|P-value:2.48E-11 ID:SGTC_73|Compound:0327-0325|FD-Score:3.54|P-value:2.34E-4 ID:SGTC_116|Compound:0155-0182|FD-Score:7.43|P-value:9.24E-14 ID:SGTC_119|Compound:0121-0043|FD-Score:3.41|P-value:3.68E-4 ID:SGTC_141|Compound:0443-0274|FD-Score:4.64|P-value:2.19E-6 ID:SGTC_267|Compound:4-Nitroquinoline-1-oxide  (4-NQO)|FD-Score:3.49|P-value:2.78E-4 ID:SGTC_275|Compound:trichlorophene|FD-Score:28.90|P-value:6.38E-181 ID:SGTC_310|Compound:1273-0058|FD-Score:8.00|P-value:1.12E-15 ID:SGTC_325|Compound:2810-4341|FD-Score:4.33|P-value:9.04E-6 ID:SGTC_341|Compound:1486-1430|FD-Score:4.37|P-value:7.43E-6 ID:SGTC_350|Compound:k064-0020|FD-Score:8.80|P-value:1.41E-18 ID:SGTC_365|Compound:0121-0052|FD-Score:20.10|P-value:9.74E-89 ID:SGTC_372|Compound:0155-0182|FD-Score:6.47|P-value:7.21E-11 ID:SGTC_383|Compound:2324-0395|FD-Score:12.80|P-value:3.21E-37 ID:SGTC_397|Compound:arbidol|FD-Score:3.20|P-value:7.80E-4 ID:SGTC_403|Compound:arphamenine a|FD-Score:-3.56|P-value:1.97E-4 ID:SGTC_431|Compound:k089-0097|FD-Score:-3.49|P-value:2.59E-4 ID:SGTC_440|Compound:fluspirilene|FD-Score:-4.23|P-value:1.27E-5 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-5.15|P-value:1.54E-7 ID:SGTC_490|Compound:phenamil|FD-Score:3.48|P-value:2.86E-4 ID:SGTC_547|Compound:1120-0019|FD-Score:7.82|P-value:4.75E-15 ID:SGTC_607|Compound:4491-0986|FD-Score:4.52|P-value:3.87E-6 ID:SGTC_617|Compound:3770-0098|FD-Score:-3.64|P-value:1.44E-4 ID:SGTC_620|Compound:0109-0052|FD-Score:4.22|P-value:1.50E-5 ID:SGTC_636|Compound:0kpi-0074|FD-Score:3.18|P-value:8.37E-4 ID:SGTC_639|Compound:0833-0042|FD-Score:-4.45|P-value:4.75E-6 ID:SGTC_647|Compound:0140-0236|FD-Score:4.74|P-value:1.35E-6 ID:SGTC_778|Compound:3456-2434|FD-Score:6.18|P-value:4.62E-10 ID:SGTC_847|Compound:0342-0274|FD-Score:4.56|P-value:3.16E-6 ID:SGTC_848|Compound:0368-0089|FD-Score:3.37|P-value:4.35E-4 ID:SGTC_851|Compound:0394-0702|FD-Score:3.94|P-value:4.90E-5 ID:SGTC_902|Compound:1120-0002|FD-Score:3.16|P-value:8.78E-4 ID:SGTC_908|Compound:3448-7491|FD-Score:10.70|P-value:1.19E-26 ID:SGTC_909|Compound:3448-9333|FD-Score:3.37|P-value:4.26E-4 ID:SGTC_1036|Compound:k216-0215|FD-Score:3.52|P-value:2.45E-4 ID:SGTC_1057|Compound:3448-5381|FD-Score:3.59|P-value:1.90E-4 ID:SGTC_1123|Compound:mangostin|FD-Score:6.29|P-value:2.36E-10 ID:SGTC_1147|Compound:3809-1045|FD-Score:3.17|P-value:8.65E-4 ID:SGTC_1154|Compound:4088-0362|FD-Score:5.87|P-value:3.03E-9 ID:SGTC_1162|Compound:k048-0130|FD-Score:-3.34|P-value:4.35E-4 ID:SGTC_1240|Compound:0356-0016|FD-Score:25.20|P-value:3.07E-138 ID:SGTC_1248|Compound:0469-0687|FD-Score:-3.33|P-value:4.54E-4 ID:SGTC_1347|Compound:1493-0251|FD-Score:11.10|P-value:2.50E-28 ID:SGTC_1353|Compound:1498-1206|FD-Score:4.13|P-value:2.20E-5 ID:SGTC_1683|Compound:st010134|FD-Score:-3.63|P-value:1.50E-4 ID:SGTC_1787|Compound:fluconazole|FD-Score:-3.72|P-value:1.06E-4 ID:SGTC_1862|Compound:st057513|FD-Score:-3.67|P-value:1.29E-4 ID:SGTC_1982|Compound:st072467|FD-Score:-3.85|P-value:6.39E-5 ID:SGTC_1988|Compound:st071872|FD-Score:3.36|P-value:4.39E-4 ID:SGTC_1998|Compound:st075797|FD-Score:9.10|P-value:9.20E-20 ID:SGTC_2037|Compound:5169046|FD-Score:3.19|P-value:8.02E-4 ID:SGTC_2098|Compound:5376439|FD-Score:3.77|P-value:9.45E-5 ID:SGTC_2408|Compound:sulbentine|FD-Score:3.87|P-value:6.37E-5 ID:SGTC_2476|Compound:5806916|FD-Score:-3.64|P-value:1.48E-4 ID:SGTC_2504|Compound:purpurogallin|FD-Score:-3.60|P-value:1.66E-4 ID:SGTC_2505|Compound:galangin|FD-Score:-3.78|P-value:8.41E-5 ID:SGTC_2542|Compound:2-hydroxy-5 (6)epoxy-tetrahydrocaryophyllene|FD-Score:4.17|P-value:1.83E-5 ID:SGTC_2613|Compound:3',4'-dimethoxydalbergione|FD-Score:3.41|P-value:3.66E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:5.74|P-value:6.48E-9 ID:SGTC_2829|Compound:7992743|FD-Score:-3.14|P-value:8.89E-4 ID:SGTC_2833|Compound:9001315|FD-Score:4.87|P-value:7.04E-7 ID:SGTC_2911|Compound:7942208|FD-Score:3.69|P-value:1.28E-4 ID:SGTC_2925|Compound:9010536|FD-Score:6.19|P-value:4.28E-10 ID:SGTC_2930|Compound:9013230|FD-Score:4.30|P-value:1.02E-5 ID:SGTC_2933|Compound:9026794|FD-Score:-3.99|P-value:3.57E-5 ID:SGTC_2965|Compound:9082772|FD-Score:10.40|P-value:2.86E-25 ID:SGTC_3062|Compound:9077494|FD-Score:6.63|P-value:2.48E-11

Top fitness defect scores for YFL036W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_275 trichlorophene 28.90 5.7 µM 6.38E-181 mitochondrial stress 2 9.31E-184
2 SGTC_1240 0356-0016 25.20 42.1 µM 3.07E-138 mitochondrial response to ROS 1 2.05E-140
3 SGTC_365 0121-0052 20.10 13.6 µM 9.74E-89 mitochondrial response to ROS 1 3.83E-90
4 SGTC_383 2324-0395 12.80 7.4 µM 3.21E-37 azole & statin 2 8.14E-38
5 SGTC_1347 1493-0251 11.10 600.0 nM 2.50E-28 mitochondrial response to ROS 1 8.79E-29
6 SGTC_908 3448-7491 10.70 47.7 µM 1.19E-26 mitochondrial stress 5 4.46E-27
7 SGTC_2965 9082772 10.40 38.6 µM 2.86E-25 mitochondrial response to ROS 2 1.13E-25
8 SGTC_1998 st075797 9.10 76.8 µM 9.20E-20 mitochondrial response to ROS 1 4.44E-20
9 SGTC_350 k064-0020 8.80 19.8 µM 1.41E-18 heme requiring 3 7.09E-19
10 SGTC_310 1273-0058 8.00 1.1 µM 1.12E-15 mitochondrial stress 89 6.30E-16
11 SGTC_547 1120-0019 7.82 610.0 nM 4.75E-15 azole & statin 2 2.73E-15
12 SGTC_116 0155-0182 7.43 31.8 µM 9.24E-14 mitochondrial response to ROS 14 5.58E-14
13 SGTC_3062 9077494 6.63 49.5 µM 2.48E-11 mitochondrial response to ROS 9 1.64E-11
14 SGTC_372 0155-0182 6.47 15.9 µM 7.21E-11 mitochondrial response to ROS 3 4.86E-11
15 SGTC_1123 mangostin 6.29 811.3 nM 2.36E-10 mitochondrial response to ROS 11 1.62E-10
16 SGTC_2925 9010536 6.19 32.5 µM 4.28E-10 azole & statin 13 2.97E-10
17 SGTC_778 3456-2434 6.18 196.0 µM 4.62E-10 mitochondrial response to ROS 24 3.21E-10
18 SGTC_1154 4088-0362 5.87 150.0 µM 3.03E-9 2 2.17E-9
19 SGTC_2688 kanamycin b 5.74 96.7 µM 6.48E-9 calcium & mitochondrial duress 174 4.70E-9
20 SGTC_2833 9001315 4.87 45.5 µM 7.04E-7 amide catabolism 30 5.53E-7

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.359 1.39E-102 YBR078W ECM33 hom GPI-anchored protein of unknown function, has a possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; phosphorylated in mitochondria; similar to Sps2p and Pst1p
0.357 3.90E-101 YOR101W RAS1 hom GTPase involved in G-protein signaling in adenylate cyclase activation; plays a role in cell proliferation; localized to the plasma membrane; homolog of mammalian RAS proto-oncogenes; relative distribution to the nucleus increases upon DNA replication stress
0.343 4.46E-93 YKL139W CTK1 hom Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I); phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity; similar to the Drosophila dCDK12 and human CDK12 and probably CDK13
0.293 1.31E-67 YDR115W YDR115W hom Putative mitochondrial ribosomal protein of the large subunit; similar to E. coli L34 ribosomal protein; required for respiratory growth, as are most mitochondrial ribosomal proteins; protein increases in abundance and relocalizes to the plasma membrane upon DNA replication stress
0.271 1.38E-57 YNR037C RSM19 hom Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S19 ribosomal protein
0.266 1.88E-55 YBL080C PET112 hom Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; mutation is functionally complemented by the bacterial GatB ortholog
0.257 8.08E-52 YIL093C RSM25 hom Mitochondrial ribosomal protein of the small subunit
0.242 8.03E-46 YGR220C MRPL9 hom Mitochondrial ribosomal protein of the large subunit
0.237 5.18E-44 YMR098C ATP25 hom Mitochondrial protein required for the stability of Oli1p (Atp9p) mRNA and for the Oli1p ring formation; YMR098C is not an essential gene
0.234 4.15E-43 YNR015W SMM1 hom Dihydrouridine synthase, member of a family of dihydrouridine synthases including Dus1p, Smm1p, Dus3p, and Dus4p; modifies uridine residues at position 20 of cytoplasmic tRNAs
0.232 3.82E-42 YLR067C PET309 hom Specific translational activator for the COX1 mRNA, also influences stability of intron-containing COX1 primary transcripts; localizes to the mitochondrial inner membrane; contains seven pentatricopeptide repeats (PPRs)
0.231 9.11E-42 YPR166C MRP2 hom Mitochondrial ribosomal protein of the small subunit
0.227 2.46E-40 YLR439W MRPL4 hom Mitochondrial ribosomal protein of the large subunit, homolog of prokaryotic L29 ribosomal protein; located at the ribosomal tunnel exit
0.226 5.38E-40 YDR337W MRPS28 hom Mitochondrial ribosomal protein of the small subunit
0.220 4.31E-38 YBR179C FZO1 hom Mitofusin; integral membrane protein involved in mitochondrial outer membrane tethering and fusion; role in mitochondrial genome maintenance; efficient tethering and degradation of Fzo1p requires an intact N-terminal GTPase domain; targeted for destruction by the ubiquitin ligase SCF-Mdm30p and the cytosolic ubiquitin-proteasome system