YFL045C / SEC53

Phosphomannomutase, involved in synthesis of GDP-mannose and dolichol-phosphate-mannose; required for folding and glycosylation of secretory proteins in the ER lumen

Zygosity: Heterozygous strain
fixedexpanded
Profile for YFL045C / SEC53

Click on Significant Values for Screen Details ID:SGTC_72|Compound:0744-0168|FD-Score:-3.12|P-value:5.21E-4 ID:SGTC_218|Compound:alverine citrate|FD-Score:-3.08|P-value:5.95E-4 ID:SGTC_335|Compound:4077-0256|FD-Score:-4.68|P-value:4.52E-7 ID:SGTC_353|Compound:1988-1175|FD-Score:-3.34|P-value:2.22E-4 ID:SGTC_674|Compound:0498-0004|FD-Score:-3.83|P-value:2.87E-5 ID:SGTC_899|Compound:1083-0020|FD-Score:-3.01|P-value:7.77E-4 ID:SGTC_1054|Compound:3429-0080|FD-Score:-4.08|P-value:9.29E-6 ID:SGTC_1081|Compound:sertraline|FD-Score:4.25|P-value:4.83E-6 ID:SGTC_1129|Compound:3453-1384|FD-Score:-3.76|P-value:3.98E-5 ID:SGTC_1148|Compound:3909-7663|FD-Score:-3.40|P-value:1.78E-4 ID:SGTC_1150|Compound:3909-7983|FD-Score:-4.37|P-value:2.29E-6 ID:SGTC_1152|Compound:3909-8331|FD-Score:4.10|P-value:9.77E-6 ID:SGTC_1227|Compound:3909-8010|FD-Score:-3.08|P-value:5.99E-4 ID:SGTC_1291|Compound:0993-0025|FD-Score:-3.27|P-value:3.02E-4 ID:SGTC_1459|Compound:k035-0031|FD-Score:5.10|P-value:5.43E-8 ID:SGTC_1478|Compound:0453-0656|FD-Score:4.61|P-value:7.72E-7 ID:SGTC_1519|Compound:esterone|FD-Score:4.38|P-value:2.55E-6 ID:SGTC_1590|Compound:st077108|FD-Score:-3.03|P-value:7.18E-4 ID:SGTC_2020|Compound:4017018|FD-Score:3.41|P-value:1.94E-4 ID:SGTC_2133|Compound:5320837|FD-Score:3.32|P-value:2.77E-4 ID:SGTC_2340|Compound:7987427|FD-Score:4.90|P-value:1.64E-7 ID:SGTC_2344|Compound:7990960|FD-Score:4.20|P-value:5.91E-6 ID:SGTC_2346|Compound:9031566|FD-Score:-2.95|P-value:9.53E-4 ID:SGTC_2349|Compound:9023730|FD-Score:4.59|P-value:8.73E-7 ID:SGTC_2497|Compound:7-hydroxyflavone|FD-Score:3.20|P-value:4.29E-4 ID:SGTC_2500|Compound:5-alpha-androstan-3,17-dione|FD-Score:3.50|P-value:1.36E-4 ID:SGTC_2532|Compound:xanthone|FD-Score:-3.14|P-value:4.84E-4 ID:SGTC_2593|Compound:nobiletin|FD-Score:-3.18|P-value:4.22E-4 ID:SGTC_2595|Compound:hexamethylquercetagetin|FD-Score:-3.00|P-value:8.00E-4 ID:SGTC_2614|Compound:arthonioic acid|FD-Score:3.96|P-value:1.89E-5 ID:SGTC_2620|Compound:irigenin|FD-Score:-4.23|P-value:4.58E-6 ID:SGTC_2642|Compound:cholecalciferol|FD-Score:4.49|P-value:1.42E-6 ID:SGTC_2706|Compound:st078239|FD-Score:3.16|P-value:4.98E-4 ID:SGTC_2823|Compound:7987624|FD-Score:2.99|P-value:9.45E-4 ID:SGTC_2851|Compound:9015922|FD-Score:4.74|P-value:3.99E-7 ID:SGTC_2965|Compound:9082772|FD-Score:-4.14|P-value:7.03E-6 ID:SGTC_3107|Compound:9121808|FD-Score:4.24|P-value:5.11E-6 ID:SGTC_3108|Compound:9121785|FD-Score:4.07|P-value:1.14E-5 ID:SGTC_3182|Compound:9108849|FD-Score:-3.63|P-value:6.82E-5 ID:SGTC_3281|Compound:9140613|FD-Score:-3.00|P-value:8.06E-4 ID:SGTC_72|Compound:0744-0168|FD-Score:-3.12|P-value:5.21E-4 ID:SGTC_218|Compound:alverine citrate|FD-Score:-3.08|P-value:5.95E-4 ID:SGTC_335|Compound:4077-0256|FD-Score:-4.68|P-value:4.52E-7 ID:SGTC_353|Compound:1988-1175|FD-Score:-3.34|P-value:2.22E-4 ID:SGTC_674|Compound:0498-0004|FD-Score:-3.83|P-value:2.87E-5 ID:SGTC_899|Compound:1083-0020|FD-Score:-3.01|P-value:7.77E-4 ID:SGTC_1054|Compound:3429-0080|FD-Score:-4.08|P-value:9.29E-6 ID:SGTC_1081|Compound:sertraline|FD-Score:4.25|P-value:4.83E-6 ID:SGTC_1129|Compound:3453-1384|FD-Score:-3.76|P-value:3.98E-5 ID:SGTC_1148|Compound:3909-7663|FD-Score:-3.40|P-value:1.78E-4 ID:SGTC_1150|Compound:3909-7983|FD-Score:-4.37|P-value:2.29E-6 ID:SGTC_1152|Compound:3909-8331|FD-Score:4.10|P-value:9.77E-6 ID:SGTC_1227|Compound:3909-8010|FD-Score:-3.08|P-value:5.99E-4 ID:SGTC_1291|Compound:0993-0025|FD-Score:-3.27|P-value:3.02E-4 ID:SGTC_1459|Compound:k035-0031|FD-Score:5.10|P-value:5.43E-8 ID:SGTC_1478|Compound:0453-0656|FD-Score:4.61|P-value:7.72E-7 ID:SGTC_1519|Compound:esterone|FD-Score:4.38|P-value:2.55E-6 ID:SGTC_1590|Compound:st077108|FD-Score:-3.03|P-value:7.18E-4 ID:SGTC_2020|Compound:4017018|FD-Score:3.41|P-value:1.94E-4 ID:SGTC_2133|Compound:5320837|FD-Score:3.32|P-value:2.77E-4 ID:SGTC_2340|Compound:7987427|FD-Score:4.90|P-value:1.64E-7 ID:SGTC_2344|Compound:7990960|FD-Score:4.20|P-value:5.91E-6 ID:SGTC_2346|Compound:9031566|FD-Score:-2.95|P-value:9.53E-4 ID:SGTC_2349|Compound:9023730|FD-Score:4.59|P-value:8.73E-7 ID:SGTC_2497|Compound:7-hydroxyflavone|FD-Score:3.20|P-value:4.29E-4 ID:SGTC_2500|Compound:5-alpha-androstan-3,17-dione|FD-Score:3.50|P-value:1.36E-4 ID:SGTC_2532|Compound:xanthone|FD-Score:-3.14|P-value:4.84E-4 ID:SGTC_2593|Compound:nobiletin|FD-Score:-3.18|P-value:4.22E-4 ID:SGTC_2595|Compound:hexamethylquercetagetin|FD-Score:-3.00|P-value:8.00E-4 ID:SGTC_2614|Compound:arthonioic acid|FD-Score:3.96|P-value:1.89E-5 ID:SGTC_2620|Compound:irigenin|FD-Score:-4.23|P-value:4.58E-6 ID:SGTC_2642|Compound:cholecalciferol|FD-Score:4.49|P-value:1.42E-6 ID:SGTC_2706|Compound:st078239|FD-Score:3.16|P-value:4.98E-4 ID:SGTC_2823|Compound:7987624|FD-Score:2.99|P-value:9.45E-4 ID:SGTC_2851|Compound:9015922|FD-Score:4.74|P-value:3.99E-7 ID:SGTC_2965|Compound:9082772|FD-Score:-4.14|P-value:7.03E-6 ID:SGTC_3107|Compound:9121808|FD-Score:4.24|P-value:5.11E-6 ID:SGTC_3108|Compound:9121785|FD-Score:4.07|P-value:1.14E-5 ID:SGTC_3182|Compound:9108849|FD-Score:-3.63|P-value:6.82E-5 ID:SGTC_3281|Compound:9140613|FD-Score:-3.00|P-value:8.06E-4

Top fitness defect scores for YFL045C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_1459 k035-0031 5.10 35.7 µM 5.43E-8 DNA intercalators 4 1.74E-7 0.05
2 SGTC_2340 7987427 4.90 183.4 µM 1.64E-7 4 4.81E-7 0.62
3 SGTC_2851 9015922 4.74 71.4 µM 3.99E-7 5 1.09E-6 0.17
4 SGTC_1478 0453-0656 4.61 35.7 µM 7.72E-7 4 2.00E-6 1.03
5 SGTC_2349 9023730 4.59 187.1 µM 8.73E-7 1 2.24E-6 0.36
6 SGTC_2642 cholecalciferol 4.49 100.0 µM 1.42E-6 2 3.51E-6 0.67
7 SGTC_1519 esterone 4.38 74.0 µM 2.55E-6 DNA intercalators 4 6.03E-6 0.05
8 SGTC_1081 sertraline 4.25 6.0 µM 4.83E-6 60S ribosome export 4 1.08E-5 0.02
9 SGTC_3107 9121808 4.24 49.5 µM 5.11E-6 3 1.14E-5 0.66
10 SGTC_2344 7990960 4.20 77.0 µM 5.91E-6 33 1.31E-5 0.01
11 SGTC_1152 3909-8331 4.10 15.9 µM 9.77E-6 5 2.07E-5 0.55
12 SGTC_3108 9121785 4.07 49.5 µM 1.14E-5 8 2.38E-5 0.00
13 SGTC_2614 arthonioic acid 3.96 13.4 µM 1.89E-5 3 3.81E-5 0.10
14 SGTC_2500 5-alpha-androstan-3,17-dione 3.50 61.4 µM 1.36E-4 7 2.36E-4 0.11
15 SGTC_2020 4017018 3.41 200.0 µM 1.94E-4 12 3.27E-4 0.11
16 SGTC_2133 5320837 3.32 200.0 µM 2.77E-4 ERG2 13 4.54E-4 0.22
17 SGTC_2497 7-hydroxyflavone 3.20 100.0 µM 4.29E-4 9 6.80E-4 0.06
18 SGTC_2706 st078239 3.16 82.5 µM 4.98E-4 9 7.80E-4 0.03
19 SGTC_2823 7987624 2.99 58.4 µM 9.45E-4 29 0.00141 0.03
20 SGTC_2894 9048625 2.97 32.5 µM 0.00100 22 0.00149 0.02

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.151 1.93E-18 YFL034C-B MOB2 het Activator of Cbk1p kinase; component of the RAM signaling network that regulates cellular polarity and morphogenesis; activation of Cbk1p facilitates the Ace2p-dependent daughter cell-specific transcription of genes involved in cell separation; similar to Mob1p
0.099 9.80E-9 YIR033W MGA2 hom ER membrane protein involved in regulation of OLE1 transcription, acts with homolog Spt23p; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting
0.097 1.65E-8 YPR059C_d YPR059C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YMC1/YPR058W
0.094 5.41E-8 YIL150C MCM10 het Essential chromatin-associated protein involved in the initiation of DNA replication; required for the association of the MCM2-7 complex with replication origins
0.092 9.32E-8 YNL026W SAM50 het Essential component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; homologous to bacterial Omp85
0.083 1.35E-6 YLR438C-A LSM3 het Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
0.083 1.49E-6 YCR036W RBK1 hom Putative ribokinase
0.083 1.76E-6 YOR345C_d YOR345C_d hom Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene REV1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2
0.082 1.84E-6 YKL192C ACP1 het Mitochondrial matrix acyl carrier protein, involved in biosynthesis of octanoate, which is a precursor to lipoic acid; activated by phosphopantetheinylation catalyzed by Ppt2p
0.082 1.88E-6 YBL013W FMT1 hom Methionyl-tRNA formyltransferase, catalyzes the formylation of initiator Met-tRNA in mitochondria; potential Cdc28p substrate
0.080 3.66E-6 YJL175W_d YJL175W_d hom Dubious open reading frame unlikely to encode a functional protein; deletion confers resistance to cisplatin, hypersensitivity to 5-fluorouracil, and growth defect at high pH with high calcium; overlaps gene for SWI3 transcription factor
0.080 4.06E-6 YJL115W ASF1 hom Nucleosome assembly factor, involved in chromatin assembly and disassembly, anti-silencing protein that causes derepression of silent loci when overexpressed; plays a role in regulating Ty1 transposition
0.080 3.99E-6 YBR196C-A_p YBR196C-A_p hom Putative protein of unknown function; identified by fungal homology and RT-PCR
0.077 9.07E-6 YOL045W PSK2 hom PAS-domain containing serine/threonine protein kinase; regulates sugar flux and translation in response to an unknown metabolite by phosphorylating Ugp1p and Gsy2p (sugar flux) and Caf20p, Tif11p and Sro9p (translation); PSK2 has a paralog, PSK1, that arose from the whole genome duplication
0.076 1.16E-5 YKR047W_d YKR047W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NAP1