YFL047W / RGD2

GTPase-activating protein (RhoGAP) for Cdc42p and Rho5p; relocalizes from bud neck to cytoplasm upon DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YFL047W / RGD2

Click on Significant Values for Screen Details ID:SGTC_66|Compound:0109-0187|FD-Score:-3.66|P-value:1.20E-4 ID:SGTC_105|Compound:0111-0055|FD-Score:-3.73|P-value:9.15E-5 ID:SGTC_260|Compound:nsc-65238|FD-Score:3.91|P-value:5.01E-5 ID:SGTC_267|Compound:4-Nitroquinoline-1-oxide  (4-NQO)|FD-Score:5.29|P-value:6.63E-8 ID:SGTC_392|Compound:N-ethylmaleimide|FD-Score:3.83|P-value:6.84E-5 ID:SGTC_394|Compound:menadione|FD-Score:-3.24|P-value:5.70E-4 ID:SGTC_406|Compound:estrone|FD-Score:3.14|P-value:8.89E-4 ID:SGTC_591|Compound:4299-0174|FD-Score:5.21|P-value:1.06E-7 ID:SGTC_593|Compound:1203-0218|FD-Score:-4.93|P-value:3.92E-7 ID:SGTC_597|Compound:k018-0003|FD-Score:3.81|P-value:7.40E-5 ID:SGTC_607|Compound:4491-0986|FD-Score:-3.21|P-value:6.31E-4 ID:SGTC_609|Compound:0986-0026|FD-Score:-3.85|P-value:5.51E-5 ID:SGTC_617|Compound:3770-0098|FD-Score:3.41|P-value:3.51E-4 ID:SGTC_618|Compound:k072-0232|FD-Score:4.54|P-value:3.11E-6 ID:SGTC_740|Compound:k014-0057|FD-Score:-3.23|P-value:5.97E-4 ID:SGTC_965|Compound:1291-5240|FD-Score:4.24|P-value:1.20E-5 ID:SGTC_996|Compound:3970-0799|FD-Score:5.68|P-value:7.48E-9 ID:SGTC_1016|Compound:4226-1401|FD-Score:3.40|P-value:3.53E-4 ID:SGTC_1023|Compound:4482-3888|FD-Score:4.37|P-value:6.69E-6 ID:SGTC_1125|Compound:tnp00248|FD-Score:3.47|P-value:2.78E-4 ID:SGTC_1303|Compound:1160-0052|FD-Score:-3.29|P-value:4.73E-4 ID:SGTC_1313|Compound:1220-0047|FD-Score:-3.47|P-value:2.45E-4 ID:SGTC_1376|Compound:2910-0861|FD-Score:3.31|P-value:4.90E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-3.45|P-value:2.67E-4 ID:SGTC_1463|Compound:k081-0032|FD-Score:-3.84|P-value:5.90E-5 ID:SGTC_1773|Compound:st048542|FD-Score:3.35|P-value:4.31E-4 ID:SGTC_1779|Compound:st039427|FD-Score:3.20|P-value:7.37E-4 ID:SGTC_1812|Compound:st052057|FD-Score:3.48|P-value:2.67E-4 ID:SGTC_2012|Compound:4023501|FD-Score:3.28|P-value:5.53E-4 ID:SGTC_2442|Compound:5158906|FD-Score:3.14|P-value:8.81E-4 ID:SGTC_2534|Compound:helenine|FD-Score:3.17|P-value:8.15E-4 ID:SGTC_2537|Compound:larixol|FD-Score:3.80|P-value:7.78E-5 ID:SGTC_2542|Compound:2-hydroxy-5 (6)epoxy-tetrahydrocaryophyllene|FD-Score:4.27|P-value:1.05E-5 ID:SGTC_2560|Compound:caryophyllene|FD-Score:-3.69|P-value:1.05E-4 ID:SGTC_2614|Compound:arthonioic acid|FD-Score:-7.22|P-value:2.33E-13 ID:SGTC_2634|Compound:4'-methoxyflavone|FD-Score:3.56|P-value:1.97E-4 ID:SGTC_2769|Compound:toremifene|FD-Score:4.62|P-value:2.06E-6 ID:SGTC_2807|Compound:7945238|FD-Score:-3.18|P-value:6.98E-4 ID:SGTC_2950|Compound:9072895|FD-Score:-5.66|P-value:7.22E-9 ID:SGTC_2995|Compound:9067767|FD-Score:-3.48|P-value:2.36E-4 ID:SGTC_2999|Compound:9069765|FD-Score:-3.84|P-value:5.88E-5 ID:SGTC_3050|Compound:9093513|FD-Score:-3.11|P-value:8.96E-4 ID:SGTC_3127|Compound:9126946|FD-Score:-3.22|P-value:6.06E-4 ID:SGTC_3190|Compound:9110366|FD-Score:3.38|P-value:3.81E-4 ID:SGTC_66|Compound:0109-0187|FD-Score:-3.66|P-value:1.20E-4 ID:SGTC_105|Compound:0111-0055|FD-Score:-3.73|P-value:9.15E-5 ID:SGTC_260|Compound:nsc-65238|FD-Score:3.91|P-value:5.01E-5 ID:SGTC_267|Compound:4-Nitroquinoline-1-oxide  (4-NQO)|FD-Score:5.29|P-value:6.63E-8 ID:SGTC_392|Compound:N-ethylmaleimide|FD-Score:3.83|P-value:6.84E-5 ID:SGTC_394|Compound:menadione|FD-Score:-3.24|P-value:5.70E-4 ID:SGTC_406|Compound:estrone|FD-Score:3.14|P-value:8.89E-4 ID:SGTC_591|Compound:4299-0174|FD-Score:5.21|P-value:1.06E-7 ID:SGTC_593|Compound:1203-0218|FD-Score:-4.93|P-value:3.92E-7 ID:SGTC_597|Compound:k018-0003|FD-Score:3.81|P-value:7.40E-5 ID:SGTC_607|Compound:4491-0986|FD-Score:-3.21|P-value:6.31E-4 ID:SGTC_609|Compound:0986-0026|FD-Score:-3.85|P-value:5.51E-5 ID:SGTC_617|Compound:3770-0098|FD-Score:3.41|P-value:3.51E-4 ID:SGTC_618|Compound:k072-0232|FD-Score:4.54|P-value:3.11E-6 ID:SGTC_740|Compound:k014-0057|FD-Score:-3.23|P-value:5.97E-4 ID:SGTC_965|Compound:1291-5240|FD-Score:4.24|P-value:1.20E-5 ID:SGTC_996|Compound:3970-0799|FD-Score:5.68|P-value:7.48E-9 ID:SGTC_1016|Compound:4226-1401|FD-Score:3.40|P-value:3.53E-4 ID:SGTC_1023|Compound:4482-3888|FD-Score:4.37|P-value:6.69E-6 ID:SGTC_1125|Compound:tnp00248|FD-Score:3.47|P-value:2.78E-4 ID:SGTC_1303|Compound:1160-0052|FD-Score:-3.29|P-value:4.73E-4 ID:SGTC_1313|Compound:1220-0047|FD-Score:-3.47|P-value:2.45E-4 ID:SGTC_1376|Compound:2910-0861|FD-Score:3.31|P-value:4.90E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-3.45|P-value:2.67E-4 ID:SGTC_1463|Compound:k081-0032|FD-Score:-3.84|P-value:5.90E-5 ID:SGTC_1773|Compound:st048542|FD-Score:3.35|P-value:4.31E-4 ID:SGTC_1779|Compound:st039427|FD-Score:3.20|P-value:7.37E-4 ID:SGTC_1812|Compound:st052057|FD-Score:3.48|P-value:2.67E-4 ID:SGTC_2012|Compound:4023501|FD-Score:3.28|P-value:5.53E-4 ID:SGTC_2442|Compound:5158906|FD-Score:3.14|P-value:8.81E-4 ID:SGTC_2534|Compound:helenine|FD-Score:3.17|P-value:8.15E-4 ID:SGTC_2537|Compound:larixol|FD-Score:3.80|P-value:7.78E-5 ID:SGTC_2542|Compound:2-hydroxy-5 (6)epoxy-tetrahydrocaryophyllene|FD-Score:4.27|P-value:1.05E-5 ID:SGTC_2560|Compound:caryophyllene|FD-Score:-3.69|P-value:1.05E-4 ID:SGTC_2614|Compound:arthonioic acid|FD-Score:-7.22|P-value:2.33E-13 ID:SGTC_2634|Compound:4'-methoxyflavone|FD-Score:3.56|P-value:1.97E-4 ID:SGTC_2769|Compound:toremifene|FD-Score:4.62|P-value:2.06E-6 ID:SGTC_2807|Compound:7945238|FD-Score:-3.18|P-value:6.98E-4 ID:SGTC_2950|Compound:9072895|FD-Score:-5.66|P-value:7.22E-9 ID:SGTC_2995|Compound:9067767|FD-Score:-3.48|P-value:2.36E-4 ID:SGTC_2999|Compound:9069765|FD-Score:-3.84|P-value:5.88E-5 ID:SGTC_3050|Compound:9093513|FD-Score:-3.11|P-value:8.96E-4 ID:SGTC_3127|Compound:9126946|FD-Score:-3.22|P-value:6.06E-4 ID:SGTC_3190|Compound:9110366|FD-Score:3.38|P-value:3.81E-4

Top fitness defect scores for YFL047W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_996 3970-0799 5.68 1.8 µM 7.48E-9 41 6.75E-9
2 SGTC_267 4-Nitroquinoline-1-oxide (4-NQO) 5.29 44.4 nM 6.63E-8 DNA damage response 21 6.03E-8
3 SGTC_591 4299-0174 5.21 154.0 µM 1.06E-7 2 9.66E-8
4 SGTC_2769 toremifene 4.62 3.7 µM 2.06E-6 11 1.89E-6
5 SGTC_618 k072-0232 4.54 33.2 µM 3.11E-6 60S ribosome export 4 2.87E-6
6 SGTC_1023 4482-3888 4.37 16.0 µM 6.69E-6 TSC3-RPN4 8 6.19E-6
7 SGTC_2542 2-hydroxy-5 (6)epoxy-tetrahydrocaryophyllene 4.27 100.0 µM 1.05E-5 13 9.76E-6
8 SGTC_965 1291-5240 4.24 308.0 µM 1.20E-5 25 1.11E-5
9 SGTC_260 nsc-65238 3.91 4.0 µM 5.01E-5 endomembrane recycling 16 4.68E-5
10 SGTC_392 N-ethylmaleimide 3.83 8.3 µM 6.84E-5 RNA pol III & RNase P/MRP 18 6.39E-5
11 SGTC_597 k018-0003 3.81 82.8 µM 7.40E-5 RNA pol III & RNase P/MRP 13 6.92E-5
12 SGTC_2537 larixol 3.80 68.9 µM 7.78E-5 25 7.28E-5
13 SGTC_2634 4'-methoxyflavone 3.56 100.0 µM 1.97E-4 45 1.85E-4
14 SGTC_1812 st052057 3.48 4.3 µM 2.67E-4 calcium & mitochondrial duress 41 2.51E-4
15 SGTC_1125 tnp00248 3.47 1.3 µM 2.78E-4 53 2.61E-4
16 SGTC_617 3770-0098 3.41 35.0 µM 3.51E-4 exosome 27 3.30E-4
17 SGTC_1016 4226-1401 3.40 38.0 µM 3.53E-4 RNA pol III & RNase P/MRP 37 3.33E-4
18 SGTC_3190 9110366 3.38 49.5 µM 3.81E-4 24 3.58E-4
19 SGTC_1773 st048542 3.35 73.6 µM 4.31E-4 73 4.06E-4
20 SGTC_1376 2910-0861 3.31 41.3 µM 4.90E-4 43 4.62E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.209 1.65E-34 YGR258C RAD2 hom Single-stranded DNA endonuclease, cleaves single-stranded DNA during nucleotide excision repair to excise damaged DNA; subunit of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPG protein
0.205 4.43E-33 YML118W NGL3 hom 3'-5' exonuclease specific for poly-A RNAs; has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; similar to Ngl1p; NGL3 has a paralog, NGL2, that arose from the whole genome duplication
0.200 1.37E-31 YER066W_p RRT13_p hom Putative protein of unknown function; non-essential gene identified in a screen for mutants with decreased levels of rDNA transcription
0.196 1.71E-30 YLR395C COX8 hom Subunit VIII of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain
0.193 1.43E-29 YDL099W BUG1 hom Cis-golgi localized protein involved in ER to Golgi transport; forms a complex with the mammalian GRASP65 homolog, Grh1p; mutants are compromised for the fusion of ER-derived vesicles with Golgi membranes
0.170 3.08E-23 YML095C RAD10 hom Single-stranded DNA endonuclease (with Rad1p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human ERCC1 protein
0.168 1.49E-22 YGL146C_p RRT6_p hom Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; contains two putative transmembrane spans, but no significant homology to other known proteins
0.157 5.60E-20 YMR201C RAD14 hom Protein that recognizes and binds damaged DNA during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); contains zinc finger motif; homolog of human XPA protein
0.147 8.96E-18 YNL220W ADE12 hom Adenylosuccinate synthase, catalyzes the first step in synthesis of adenosine monophosphate from inosine 5'monophosphate during purine nucleotide biosynthesis; exhibits binding to single-stranded autonomously replicating (ARS) core sequence
0.147 9.18E-18 YGR015C_p YGR015C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion
0.145 2.64E-17 YGL131C SNT2 hom DNA binding protein with similarity to the S. pombe Snt2 protein; RING finger ubiquitin ligase (E3) involved in ubiquitylation and degradation of excess histones; interacts with Ubc4p; null mutant sensitive to hydroxyurea (HU); computational analysis suggests that Snt2p is a transcription factor with a role in regulation of expression of genes encoding amine transporters; relocalizes from nucleus to cytoplasm upon DNA replication stress
0.141 2.35E-16 YDL186W_p YDL186W_p hom Putative protein of unknown function; YDL186W is not an essential gene
0.140 4.34E-16 YPR180W AOS1 het Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with Uba2p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability
0.135 4.08E-15 YOR300W_d YOR300W_d hom Dubious open reading frame, unlikely to encode a protein; overlaps with verified gene BUD7/YOR299W; mutation affects bipolar budding and bud site selection, though phenotype could be due to the mutation's effects on BUD7
0.135 5.41E-15 YDR505C PSP1 hom Asn and gln rich protein of unknown function; high-copy suppressor of POL1 (DNA polymerase alpha) and partial suppressor of CDC2 (polymerase delta) and CDC6 (pre-RC loading factor) mutations; overexpression results in growth inhibition; PSP1 has a paralog, YLR177W, that arose from the whole genome duplication