YFR004W / RPN11

Metalloprotease subunit of the 19S regulatory particle of the 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress

Zygosity: Heterozygous strain
fixedexpanded
Profile for YFR004W / RPN11

Click on Significant Values for Screen Details ID:SGTC_73|Compound:0327-0325|FD-Score:-4.23|P-value:5.13E-5 ID:SGTC_339|Compound:3091-4833|FD-Score:3.83|P-value:1.87E-4 ID:SGTC_359|Compound:0081-0058|FD-Score:4.59|P-value:1.07E-5 ID:SGTC_410|Compound:α-linolenic acid|FD-Score:3.91|P-value:1.46E-4 ID:SGTC_475|Compound:α-linolenic acid|FD-Score:3.70|P-value:3.00E-4 ID:SGTC_594|Compound:1487-1584|FD-Score:3.33|P-value:9.84E-4 ID:SGTC_733|Compound:4069-0007|FD-Score:-6.33|P-value:3.00E-9 ID:SGTC_744|Compound:1594-0068|FD-Score:4.51|P-value:1.48E-5 ID:SGTC_767|Compound:0929-0023|FD-Score:-3.41|P-value:8.94E-4 ID:SGTC_834|Compound:0312-0008|FD-Score:-3.68|P-value:3.61E-4 ID:SGTC_908|Compound:3448-7491|FD-Score:-3.38|P-value:9.73E-4 ID:SGTC_912|Compound:3474-0017|FD-Score:3.42|P-value:7.57E-4 ID:SGTC_920|Compound:mycophenolic acid|FD-Score:-4.33|P-value:3.52E-5 ID:SGTC_942|Compound:3253-1115|FD-Score:4.19|P-value:5.15E-5 ID:SGTC_977|Compound:1348-1378|FD-Score:-3.70|P-value:3.49E-4 ID:SGTC_1009|Compound:4106-0057|FD-Score:3.61|P-value:3.99E-4 ID:SGTC_1016|Compound:4226-1401|FD-Score:6.75|P-value:2.10E-10 ID:SGTC_1026|Compound:k007-1105|FD-Score:-4.09|P-value:8.52E-5 ID:SGTC_1084|Compound:voriconazole|FD-Score:3.49|P-value:6.07E-4 ID:SGTC_1131|Compound:3454-3128|FD-Score:4.74|P-value:5.54E-6 ID:SGTC_1240|Compound:0356-0016|FD-Score:-4.28|P-value:4.26E-5 ID:SGTC_1268|Compound:0828-0275|FD-Score:3.44|P-value:7.12E-4 ID:SGTC_1347|Compound:1493-0251|FD-Score:-6.33|P-value:2.95E-9 ID:SGTC_1353|Compound:1498-1206|FD-Score:-3.56|P-value:5.39E-4 ID:SGTC_1378|Compound:2093-0007|FD-Score:3.61|P-value:4.07E-4 ID:SGTC_1511|Compound:0906-3109|FD-Score:-4.00|P-value:1.21E-4 ID:SGTC_1516|Compound:st013856|FD-Score:3.54|P-value:5.04E-4 ID:SGTC_1686|Compound:st019653|FD-Score:3.72|P-value:2.82E-4 ID:SGTC_1716|Compound:st033144|FD-Score:4.61|P-value:9.77E-6 ID:SGTC_1737|Compound:st035557|FD-Score:-3.49|P-value:6.94E-4 ID:SGTC_1809|Compound:st050081|FD-Score:4.31|P-value:3.16E-5 ID:SGTC_1918|Compound:oxolan-2-ylmethyl 3-bromobenzoate|FD-Score:4.49|P-value:1.58E-5 ID:SGTC_2004|Compound:4023728|FD-Score:7.13|P-value:2.14E-11 ID:SGTC_2054|Compound:5233173|FD-Score:-3.88|P-value:1.83E-4 ID:SGTC_2124|Compound:5246320|FD-Score:7.56|P-value:1.37E-12 ID:SGTC_2129|Compound:girgensonine|FD-Score:4.16|P-value:5.65E-5 ID:SGTC_2306|Compound:7482397|FD-Score:4.05|P-value:8.67E-5 ID:SGTC_2310|Compound:7668677|FD-Score:4.27|P-value:3.78E-5 ID:SGTC_2318|Compound:6029776|FD-Score:-3.39|P-value:9.45E-4 ID:SGTC_2321|Compound:7390090|FD-Score:-5.23|P-value:7.58E-7 ID:SGTC_2322|Compound:7413319|FD-Score:-10.80|P-value:1.29E-23 ID:SGTC_2348|Compound:9022543|FD-Score:4.57|P-value:1.14E-5 ID:SGTC_2399|Compound:5884133|FD-Score:4.71|P-value:6.33E-6 ID:SGTC_2519|Compound:cryptotanshinone|FD-Score:5.99|P-value:1.49E-8 ID:SGTC_2531|Compound:methyl deoxycholate|FD-Score:-4.56|P-value:1.38E-5 ID:SGTC_2538|Compound:3',7'-epoxycaryophyllan-6-one|FD-Score:4.09|P-value:7.55E-5 ID:SGTC_2539|Compound:epi(13)torulosol|FD-Score:-5.47|P-value:2.54E-7 ID:SGTC_2617|Compound:dihydrotanshinone i|FD-Score:3.66|P-value:3.47E-4 ID:SGTC_2651|Compound:1327-0036|FD-Score:-4.66|P-value:9.21E-6 ID:SGTC_2662|Compound:daunorubicin|FD-Score:-4.38|P-value:2.87E-5 ID:SGTC_2666|Compound:tannic acid|FD-Score:-3.63|P-value:4.32E-4 ID:SGTC_2790|Compound:5423970|FD-Score:4.39|P-value:2.33E-5 ID:SGTC_2890|Compound:9046729|FD-Score:-3.84|P-value:2.09E-4 ID:SGTC_2896|Compound:9052656|FD-Score:-6.10|P-value:1.04E-8 ID:SGTC_2921|Compound:7974240|FD-Score:4.00|P-value:1.02E-4 ID:SGTC_2923|Compound:7992654|FD-Score:-3.41|P-value:8.95E-4 ID:SGTC_2925|Compound:9010536|FD-Score:-6.35|P-value:2.63E-9 ID:SGTC_2926|Compound:9010726|FD-Score:-4.39|P-value:2.81E-5 ID:SGTC_2965|Compound:9082772|FD-Score:-4.31|P-value:3.83E-5 ID:SGTC_3000|Compound:9075062|FD-Score:-5.98|P-value:1.92E-8 ID:SGTC_3015|Compound:9080845|FD-Score:4.92|P-value:2.61E-6 ID:SGTC_3062|Compound:9077494|FD-Score:-5.31|P-value:5.32E-7 ID:SGTC_3142|Compound:9086498|FD-Score:6.96|P-value:6.04E-11 ID:SGTC_73|Compound:0327-0325|FD-Score:-4.23|P-value:5.13E-5 ID:SGTC_339|Compound:3091-4833|FD-Score:3.83|P-value:1.87E-4 ID:SGTC_359|Compound:0081-0058|FD-Score:4.59|P-value:1.07E-5 ID:SGTC_410|Compound:α-linolenic acid|FD-Score:3.91|P-value:1.46E-4 ID:SGTC_475|Compound:α-linolenic acid|FD-Score:3.70|P-value:3.00E-4 ID:SGTC_594|Compound:1487-1584|FD-Score:3.33|P-value:9.84E-4 ID:SGTC_733|Compound:4069-0007|FD-Score:-6.33|P-value:3.00E-9 ID:SGTC_744|Compound:1594-0068|FD-Score:4.51|P-value:1.48E-5 ID:SGTC_767|Compound:0929-0023|FD-Score:-3.41|P-value:8.94E-4 ID:SGTC_834|Compound:0312-0008|FD-Score:-3.68|P-value:3.61E-4 ID:SGTC_908|Compound:3448-7491|FD-Score:-3.38|P-value:9.73E-4 ID:SGTC_912|Compound:3474-0017|FD-Score:3.42|P-value:7.57E-4 ID:SGTC_920|Compound:mycophenolic acid|FD-Score:-4.33|P-value:3.52E-5 ID:SGTC_942|Compound:3253-1115|FD-Score:4.19|P-value:5.15E-5 ID:SGTC_977|Compound:1348-1378|FD-Score:-3.70|P-value:3.49E-4 ID:SGTC_1009|Compound:4106-0057|FD-Score:3.61|P-value:3.99E-4 ID:SGTC_1016|Compound:4226-1401|FD-Score:6.75|P-value:2.10E-10 ID:SGTC_1026|Compound:k007-1105|FD-Score:-4.09|P-value:8.52E-5 ID:SGTC_1084|Compound:voriconazole|FD-Score:3.49|P-value:6.07E-4 ID:SGTC_1131|Compound:3454-3128|FD-Score:4.74|P-value:5.54E-6 ID:SGTC_1240|Compound:0356-0016|FD-Score:-4.28|P-value:4.26E-5 ID:SGTC_1268|Compound:0828-0275|FD-Score:3.44|P-value:7.12E-4 ID:SGTC_1347|Compound:1493-0251|FD-Score:-6.33|P-value:2.95E-9 ID:SGTC_1353|Compound:1498-1206|FD-Score:-3.56|P-value:5.39E-4 ID:SGTC_1378|Compound:2093-0007|FD-Score:3.61|P-value:4.07E-4 ID:SGTC_1511|Compound:0906-3109|FD-Score:-4.00|P-value:1.21E-4 ID:SGTC_1516|Compound:st013856|FD-Score:3.54|P-value:5.04E-4 ID:SGTC_1686|Compound:st019653|FD-Score:3.72|P-value:2.82E-4 ID:SGTC_1716|Compound:st033144|FD-Score:4.61|P-value:9.77E-6 ID:SGTC_1737|Compound:st035557|FD-Score:-3.49|P-value:6.94E-4 ID:SGTC_1809|Compound:st050081|FD-Score:4.31|P-value:3.16E-5 ID:SGTC_1918|Compound:oxolan-2-ylmethyl 3-bromobenzoate|FD-Score:4.49|P-value:1.58E-5 ID:SGTC_2004|Compound:4023728|FD-Score:7.13|P-value:2.14E-11 ID:SGTC_2054|Compound:5233173|FD-Score:-3.88|P-value:1.83E-4 ID:SGTC_2124|Compound:5246320|FD-Score:7.56|P-value:1.37E-12 ID:SGTC_2129|Compound:girgensonine|FD-Score:4.16|P-value:5.65E-5 ID:SGTC_2306|Compound:7482397|FD-Score:4.05|P-value:8.67E-5 ID:SGTC_2310|Compound:7668677|FD-Score:4.27|P-value:3.78E-5 ID:SGTC_2318|Compound:6029776|FD-Score:-3.39|P-value:9.45E-4 ID:SGTC_2321|Compound:7390090|FD-Score:-5.23|P-value:7.58E-7 ID:SGTC_2322|Compound:7413319|FD-Score:-10.80|P-value:1.29E-23 ID:SGTC_2348|Compound:9022543|FD-Score:4.57|P-value:1.14E-5 ID:SGTC_2399|Compound:5884133|FD-Score:4.71|P-value:6.33E-6 ID:SGTC_2519|Compound:cryptotanshinone|FD-Score:5.99|P-value:1.49E-8 ID:SGTC_2531|Compound:methyl deoxycholate|FD-Score:-4.56|P-value:1.38E-5 ID:SGTC_2538|Compound:3',7'-epoxycaryophyllan-6-one|FD-Score:4.09|P-value:7.55E-5 ID:SGTC_2539|Compound:epi(13)torulosol|FD-Score:-5.47|P-value:2.54E-7 ID:SGTC_2617|Compound:dihydrotanshinone i|FD-Score:3.66|P-value:3.47E-4 ID:SGTC_2651|Compound:1327-0036|FD-Score:-4.66|P-value:9.21E-6 ID:SGTC_2662|Compound:daunorubicin|FD-Score:-4.38|P-value:2.87E-5 ID:SGTC_2666|Compound:tannic acid|FD-Score:-3.63|P-value:4.32E-4 ID:SGTC_2790|Compound:5423970|FD-Score:4.39|P-value:2.33E-5 ID:SGTC_2890|Compound:9046729|FD-Score:-3.84|P-value:2.09E-4 ID:SGTC_2896|Compound:9052656|FD-Score:-6.10|P-value:1.04E-8 ID:SGTC_2921|Compound:7974240|FD-Score:4.00|P-value:1.02E-4 ID:SGTC_2923|Compound:7992654|FD-Score:-3.41|P-value:8.95E-4 ID:SGTC_2925|Compound:9010536|FD-Score:-6.35|P-value:2.63E-9 ID:SGTC_2926|Compound:9010726|FD-Score:-4.39|P-value:2.81E-5 ID:SGTC_2965|Compound:9082772|FD-Score:-4.31|P-value:3.83E-5 ID:SGTC_3000|Compound:9075062|FD-Score:-5.98|P-value:1.92E-8 ID:SGTC_3015|Compound:9080845|FD-Score:4.92|P-value:2.61E-6 ID:SGTC_3062|Compound:9077494|FD-Score:-5.31|P-value:5.32E-7 ID:SGTC_3142|Compound:9086498|FD-Score:6.96|P-value:6.04E-11

Top fitness defect scores for YFR004W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2124 5246320 7.56 200.0 µM 1.37E-12 heme biosynthesis & mitochondrial translocase 12 2.09E-14 1.23
2 SGTC_2004 4023728 7.13 41.9 µM 2.14E-11 12 5.17E-13 0.22
3 SGTC_3142 9086498 6.96 49.5 µM 6.04E-11 iron homeostasis 19 1.74E-12 0.34
4 SGTC_1016 4226-1401 6.75 38.0 µM 2.10E-10 RNA pol III & RNase P/MRP 6 7.42E-12 0.64
5 SGTC_2519 cryptotanshinone 5.99 88.0 µM 1.49E-8 heme biosynthesis & mitochondrial translocase 8 1.07E-9 0.42
6 SGTC_3015 9080845 4.92 49.5 µM 2.61E-6 2 4.40E-7 0.72
7 SGTC_1131 3454-3128 4.74 32.3 µM 5.54E-6 7 1.06E-6 0.09
8 SGTC_2399 5884133 4.71 200.0 µM 6.33E-6 cell wall signaling 11 1.23E-6 0.14
9 SGTC_1716 st033144 4.61 30.6 µM 9.77E-6 5 2.04E-6 0.48
10 SGTC_359 0081-0058 4.59 15.9 µM 1.07E-5 60S ribosome export 9 2.26E-6 0.27
11 SGTC_2348 9022543 4.57 33.4 µM 1.14E-5 mitochondrial processes 25 2.44E-6 0.02
12 SGTC_744 1594-0068 4.51 283.0 µM 1.48E-5 amide catabolism 2 3.32E-6 0.15
13 SGTC_1918 oxolan-2-ylmethyl 3-bromobenzoate 4.49 70.1 µM 1.58E-5 2 3.58E-6 0.36
14 SGTC_2790 5423970 4.39 71.4 µM 2.33E-5 endomembrane recycling 13 5.61E-6 0.06
15 SGTC_1809 st050081 4.31 57.6 µM 3.16E-5 5 7.99E-6 0.45
16 SGTC_2310 7668677 4.27 200.0 µM 3.78E-5 4 9.83E-6 0.17
17 SGTC_942 3253-1115 4.19 227.0 µM 5.15E-5 4 1.41E-5 0.30
18 SGTC_2129 girgensonine 4.16 121.0 µM 5.65E-5 heme biosynthesis & mitochondrial translocase 20 1.57E-5 0.03
19 SGTC_2538 3',7'-epoxycaryophyllan-6-one 4.09 100.0 µM 7.55E-5 9 2.19E-5 0.02
20 SGTC_2306 7482397 4.05 179.5 µM 8.67E-5 RPP1 & pyrimidine depletion 10 2.57E-5 0.29

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.282 4.84E-62 YOR117W RPT5 het One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1
0.236 1.29E-43 YER021W RPN3 het Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control
0.228 1.01E-40 YDL007W RPT2 het One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for normal peptide hydrolysis by the core 20S particle
0.224 4.33E-39 YDL097C RPN6 het Essential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress
0.219 1.11E-37 YGL048C RPT6 het ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle; protein abundance increases in response to DNA replication stress
0.195 6.20E-30 YDR427W RPN9 het Non-ATPase regulatory subunit of the 26S proteasome; has similarity to putative proteasomal subunits in other species; null mutant is temperature sensitive and exhibits cell cycle and proteasome assembly defects; protein abundance increases in response to DNA replication stress
0.193 1.74E-29 YFR052W RPN12 het Subunit of the 19S regulatory particle of the 26S proteasome lid; synthetically lethal with RPT1, which is an ATPase component of the 19S regulatory particle; physically interacts with Nob1p and Rpn3p; protein abundance increases in response to DNA replication stress
0.192 4.56E-29 YDL147W RPN5 het Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein
0.145 3.53E-17 YFR050C PRE4 het Beta 7 subunit of the 20S proteasome
0.143 8.61E-17 YOR261C RPN8 het Essential, non-ATPase regulatory subunit of the 26S proteasome; has similarity to the human p40 proteasomal subunit and to another S. cerevisiae regulatory subunit, Rpn11p
0.142 1.92E-16 YDR527W RBA50 het Protein involved in transcription; interacts with RNA polymerase II subunits Rpb2p, Rpb3, and Rpb11p; has similarity to human RPAP1
0.141 3.24E-16 YDR394W RPT3 het One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B
0.136 2.79E-15 YBL041W PRE7 het Beta 6 subunit of the 20S proteasome
0.126 2.67E-13 YOR259C RPT4 het One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in degradation of ubiquitinated substrates; contributes preferentially to ERAD; required for spindle pole body duplication; mainly nuclear localization
0.115 2.24E-11 YOL038W PRE6 het Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress