YFR005C / SAD1

Conserved zinc-finger domain protein involved in pre-mRNA splicing, required for assembly of U4 snRNA into the U4/U6 particle

Zygosity: Heterozygous strain
fixedexpanded
Profile for YFR005C / SAD1

Click on Significant Values for Screen Details ID:SGTC_13|Compound:0199-0087|FD-Score:3.50|P-value:4.50E-4 ID:SGTC_573|Compound:3702-0553|FD-Score:3.78|P-value:1.70E-4 ID:SGTC_890|Compound:0929-0006|FD-Score:3.84|P-value:1.33E-4 ID:SGTC_1046|Compound:2001-0062|FD-Score:4.26|P-value:2.63E-5 ID:SGTC_1486|Compound:3970-0793|FD-Score:3.52|P-value:4.21E-4 ID:SGTC_1628|Compound:st004252|FD-Score:4.97|P-value:1.14E-6 ID:SGTC_1991|Compound:st073000|FD-Score:-3.54|P-value:3.29E-4 ID:SGTC_2000|Compound:st075254|FD-Score:-3.57|P-value:2.96E-4 ID:SGTC_2026|Compound:5148700|FD-Score:3.93|P-value:9.56E-5 ID:SGTC_2070|Compound:5230947|FD-Score:3.52|P-value:4.19E-4 ID:SGTC_2147|Compound:5545932|FD-Score:4.36|P-value:1.73E-5 ID:SGTC_2362|Compound:9071156|FD-Score:4.21|P-value:3.16E-5 ID:SGTC_2706|Compound:st078239|FD-Score:3.33|P-value:8.08E-4 ID:SGTC_2858|Compound:9025781|FD-Score:3.64|P-value:2.81E-4 ID:SGTC_2881|Compound:9058953|FD-Score:4.01|P-value:6.94E-5 ID:SGTC_3137|Compound:9097352|FD-Score:-3.65|P-value:2.19E-4 ID:SGTC_3217|Compound:9129673|FD-Score:-4.34|P-value:1.51E-5 ID:SGTC_3289|Compound:9119912|FD-Score:-3.30|P-value:7.42E-4 ID:SGTC_3314|Compound:9135860|FD-Score:-3.49|P-value:3.88E-4 ID:SGTC_13|Compound:0199-0087|FD-Score:3.50|P-value:4.50E-4 ID:SGTC_573|Compound:3702-0553|FD-Score:3.78|P-value:1.70E-4 ID:SGTC_890|Compound:0929-0006|FD-Score:3.84|P-value:1.33E-4 ID:SGTC_1046|Compound:2001-0062|FD-Score:4.26|P-value:2.63E-5 ID:SGTC_1486|Compound:3970-0793|FD-Score:3.52|P-value:4.21E-4 ID:SGTC_1628|Compound:st004252|FD-Score:4.97|P-value:1.14E-6 ID:SGTC_1991|Compound:st073000|FD-Score:-3.54|P-value:3.29E-4 ID:SGTC_2000|Compound:st075254|FD-Score:-3.57|P-value:2.96E-4 ID:SGTC_2026|Compound:5148700|FD-Score:3.93|P-value:9.56E-5 ID:SGTC_2070|Compound:5230947|FD-Score:3.52|P-value:4.19E-4 ID:SGTC_2147|Compound:5545932|FD-Score:4.36|P-value:1.73E-5 ID:SGTC_2362|Compound:9071156|FD-Score:4.21|P-value:3.16E-5 ID:SGTC_2706|Compound:st078239|FD-Score:3.33|P-value:8.08E-4 ID:SGTC_2858|Compound:9025781|FD-Score:3.64|P-value:2.81E-4 ID:SGTC_2881|Compound:9058953|FD-Score:4.01|P-value:6.94E-5 ID:SGTC_3137|Compound:9097352|FD-Score:-3.65|P-value:2.19E-4 ID:SGTC_3217|Compound:9129673|FD-Score:-4.34|P-value:1.51E-5 ID:SGTC_3289|Compound:9119912|FD-Score:-3.30|P-value:7.42E-4 ID:SGTC_3314|Compound:9135860|FD-Score:-3.49|P-value:3.88E-4

Top fitness defect scores for YFR005C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_1628 st004252 4.97 77.7 µM 1.14E-6 3 3.29E-7 0.07
2 SGTC_2147 5545932 4.36 89.7 µM 1.73E-5 ergosterol biosynthesis 9 6.50E-6 0.04
3 SGTC_1046 2001-0062 4.26 216.0 µM 2.63E-5 1 1.03E-5 0.40
4 SGTC_2362 9071156 4.21 126.0 µM 3.16E-5 1 1.26E-5 0.28
5 SGTC_2881 9058953 4.01 52.0 µM 6.94E-5 5 2.99E-5 0.20
6 SGTC_2026 5148700 3.93 152.0 µM 9.56E-5 5 4.24E-5 0.06
7 SGTC_890 0929-0006 3.84 47.2 µM 1.33E-4 3 6.09E-5 0.74
8 SGTC_573 3702-0553 3.78 84.7 µM 1.70E-4 3 7.96E-5 0.02
9 SGTC_2858 9025781 3.64 9.0 µM 2.81E-4 sphingolipid biosynthesis & PDR1 17 1.38E-4 0.06
10 SGTC_2070 5230947 3.52 200.0 µM 4.19E-4 2 2.14E-4 0.08
11 SGTC_1486 3970-0793 3.52 4.5 µM 4.21E-4 5 2.15E-4 0.22
12 SGTC_13 0199-0087 3.50 65.0 µM 4.50E-4 ERG2 5 2.32E-4 0.00
13 SGTC_2706 st078239 3.33 82.5 µM 8.08E-4 6 4.40E-4 0.00
14 SGTC_1523 st012844 3.26 44.7 µM 0.00102 60S ribosome export 8 5.66E-4 0.00
15 SGTC_120 rhodamine 6G 3.25 52.3 µM 0.00102 mitochondrial processes 30 5.71E-4 0.01
16 SGTC_1465 k213-0056 3.23 144.0 µM 0.00109 DNA intercalators 21 6.08E-4 0.04
17 SGTC_2535 4',4'-dimethoxydalbergione 3.07 51.2 µM 0.00183 DNA damage response 8 0.00108 0.05
18 SGTC_1678 st019265 3.05 80.2 µM 0.00193 60S ribosome export 15 0.00114 0.05
19 SGTC_1724 st037281 3.05 8.3 µM 0.00195 27 0.00116 0.02
20 SGTC_3337 9144342 3.04 33.5 µM 0.00202 21 0.00120 0.03

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.131 2.44E-14 YKR061W KTR2 hom Mannosyltransferase involved in N-linked protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; KTR2 has a paralog, YUR1, that arose from the whole genome duplication
0.116 1.51E-11 YNL193W_p YNL193W_p hom Putative protein of unknown function; exhibits a two-hybrid interaction with Yhr151cp in a large-scale analysis
0.108 2.96E-10 YPL217C BMS1 het GTPase required for synthesis of 40S ribosomal subunits and for processing the 35S pre-rRNA at sites A0, A1, and A2; interacts with Rcl1p, which stimulates its GTPase and U3 snoRNA binding activities; has similarity to Tsr1p
0.103 2.44E-9 YLR421C RPN13 hom Subunit of the 19S regulatory particle of the 26S proteasome lid; acts as a ubiquitin receptor for the proteasome; null mutants accumulate ubiquitinated Gcn4p and display decreased 26S proteasome stability; protein abundance increases in response to DNA replication stress
0.096 2.70E-8 YMR080C NAM7 hom ATP-dependent RNA helicase of the SFI superfamily; involved in nonsense mediated mRNA decay; required for efficient translation termination at nonsense codons and targeting of NMD substrates to P-bodies; involved in telomere maintenance; forms cytoplasmic foci upon DNA replication stress
0.093 6.34E-8 YNL047C SLM2 hom Phosphoinositide PI4,5P(2) binding protein, forms a complex with Slm1p; acts downstream of Mss4p in a pathway regulating actin cytoskeleton organization in response to stress; phosphorylated by the TORC2 complex; SLM2 has a paralog, SLM1, that arose from the whole genome duplication
0.092 9.45E-8 YDL123W SNA4 hom Protein of unknown function, localized to the vacuolar outer membrane; predicted to be palmitoylated
0.090 1.86E-7 YPR191W QCR2 hom Subunit 2 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; phosphorylated; transcription is regulated by Hap1p, Hap2p/Hap3p, and heme
0.089 2.09E-7 YGL066W SGF73 hom SAGA complex subunit; has a role in anchoring the deubiquitination module into SAGA and SLIK complexes; involved in preinitiation complex assembly at promoters; human ortholog ataxin-7 is associated with spinocerebellar ataxia diseases; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay
0.088 3.10E-7 YGL032C AGA2 hom Adhesion subunit of a-agglutinin of a-cells, C-terminal sequence acts as a ligand for alpha-agglutinin (Sag1p) during agglutination, modified with O-linked oligomannosyl chains, linked to anchorage subunit Aga1p via two disulfide bonds
0.081 2.56E-6 YNR013C PHO91 hom Low-affinity phosphate transporter of the vacuolar membrane; deletion of pho84, pho87, pho89, pho90, and pho91 causes synthetic lethality; transcription independent of Pi and Pho4p activity; overexpression results in vigorous growth
0.080 3.99E-6 YNL237W YTP1 hom Probable type-III integral membrane protein of unknown function, has regions of similarity to mitochondrial electron transport proteins
0.079 4.49E-6 YPL001W HAT1 hom Catalytic subunit of the Hat1p-Hat2p histone acetyltransferase complex that uses the cofactor acetyl coenzyme A, to acetylate free nuclear and cytoplasmic histone H4; involved in telomeric silencing and DNA double-strand break repair
0.078 6.44E-6 YDR100W TVP15 hom Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p
0.078 6.79E-6 YOR353C SOG2 het Key component of the RAM signaling network, required for proper cell morphogenesis and cell separation after mitosis