YFR010W / UBP6

Ubiquitin-specific protease situated in the base subcomplex of the 26S proteasome, releases free ubiquitin from branched polyubiquitin chains; works in opposition to Hul5p polyubiquitin elongation activity; mutant has aneuploidy tolerance

Zygosity: Homozygous strain
fixedexpanded
Profile for YFR010W / UBP6

Click on Significant Values for Screen Details ID:SGTC_58|Compound:0957-0393|FD-Score:7.67|P-value:1.43E-10 ID:SGTC_98|Compound:0074-0023|FD-Score:3.97|P-value:5.61E-4 ID:SGTC_126|Compound:0417-1624|FD-Score:4.16|P-value:3.11E-4 ID:SGTC_159|Compound:3970-0959|FD-Score:7.02|P-value:3.91E-9 ID:SGTC_214|Compound:4373-3496|FD-Score:5.51|P-value:3.00E-6 ID:SGTC_246|Compound:cycloheximide|FD-Score:4.35|P-value:1.75E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:4.11|P-value:3.64E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-4.32|P-value:1.86E-4 ID:SGTC_488|Compound:nitrendipine|FD-Score:4.38|P-value:1.57E-4 ID:SGTC_690|Compound:glyoxal|FD-Score:5.80|P-value:9.20E-7 ID:SGTC_818|Compound:4072-2707|FD-Score:4.30|P-value:2.07E-4 ID:SGTC_851|Compound:0394-0702|FD-Score:4.36|P-value:1.72E-4 ID:SGTC_1017|Compound:4239-0030|FD-Score:4.18|P-value:2.97E-4 ID:SGTC_1086|Compound:cerivastatin|FD-Score:4.12|P-value:3.61E-4 ID:SGTC_1126|Compound:isoteolin|FD-Score:3.99|P-value:5.24E-4 ID:SGTC_1452|Compound:4194-0072|FD-Score:4.11|P-value:3.72E-4 ID:SGTC_1661|Compound:st013063|FD-Score:5.16|P-value:1.12E-5 ID:SGTC_1722|Compound:st034304|FD-Score:4.20|P-value:2.84E-4 ID:SGTC_1787|Compound:fluconazole|FD-Score:-3.94|P-value:5.73E-4 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:5.12|P-value:1.28E-5 ID:SGTC_2009|Compound:4012297|FD-Score:4.75|P-value:4.83E-5 ID:SGTC_2060|Compound:5238662|FD-Score:6.16|P-value:2.03E-7 ID:SGTC_2274|Compound:7947094|FD-Score:4.60|P-value:7.84E-5 ID:SGTC_2369|Compound:9071472|FD-Score:4.11|P-value:3.67E-4 ID:SGTC_2399|Compound:5884133|FD-Score:4.91|P-value:2.71E-5 ID:SGTC_2521|Compound:angolensin|FD-Score:3.99|P-value:5.24E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:6.17|P-value:1.95E-7 ID:SGTC_2688|Compound:kanamycin b|FD-Score:-4.70|P-value:5.39E-5 ID:SGTC_2862|Compound:9027634|FD-Score:4.63|P-value:7.16E-5 ID:SGTC_2872|Compound:9044037|FD-Score:3.88|P-value:7.24E-4 ID:SGTC_2936|Compound:9038235|FD-Score:3.91|P-value:6.53E-4 ID:SGTC_2942|Compound:9052777|FD-Score:4.43|P-value:1.38E-4 ID:SGTC_3006|Compound:9075424|FD-Score:-5.09|P-value:1.35E-5 ID:SGTC_3018|Compound:9082602|FD-Score:-5.05|P-value:1.56E-5 ID:SGTC_3052|Compound:9092717|FD-Score:4.29|P-value:2.11E-4 ID:SGTC_3219|Compound:9131112|FD-Score:-4.38|P-value:1.52E-4 ID:SGTC_3328|Compound:9140624|FD-Score:4.49|P-value:1.13E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:7.67|P-value:1.43E-10 ID:SGTC_98|Compound:0074-0023|FD-Score:3.97|P-value:5.61E-4 ID:SGTC_126|Compound:0417-1624|FD-Score:4.16|P-value:3.11E-4 ID:SGTC_159|Compound:3970-0959|FD-Score:7.02|P-value:3.91E-9 ID:SGTC_214|Compound:4373-3496|FD-Score:5.51|P-value:3.00E-6 ID:SGTC_246|Compound:cycloheximide|FD-Score:4.35|P-value:1.75E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:4.11|P-value:3.64E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-4.32|P-value:1.86E-4 ID:SGTC_488|Compound:nitrendipine|FD-Score:4.38|P-value:1.57E-4 ID:SGTC_690|Compound:glyoxal|FD-Score:5.80|P-value:9.20E-7 ID:SGTC_818|Compound:4072-2707|FD-Score:4.30|P-value:2.07E-4 ID:SGTC_851|Compound:0394-0702|FD-Score:4.36|P-value:1.72E-4 ID:SGTC_1017|Compound:4239-0030|FD-Score:4.18|P-value:2.97E-4 ID:SGTC_1086|Compound:cerivastatin|FD-Score:4.12|P-value:3.61E-4 ID:SGTC_1126|Compound:isoteolin|FD-Score:3.99|P-value:5.24E-4 ID:SGTC_1452|Compound:4194-0072|FD-Score:4.11|P-value:3.72E-4 ID:SGTC_1661|Compound:st013063|FD-Score:5.16|P-value:1.12E-5 ID:SGTC_1722|Compound:st034304|FD-Score:4.20|P-value:2.84E-4 ID:SGTC_1787|Compound:fluconazole|FD-Score:-3.94|P-value:5.73E-4 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:5.12|P-value:1.28E-5 ID:SGTC_2009|Compound:4012297|FD-Score:4.75|P-value:4.83E-5 ID:SGTC_2060|Compound:5238662|FD-Score:6.16|P-value:2.03E-7 ID:SGTC_2274|Compound:7947094|FD-Score:4.60|P-value:7.84E-5 ID:SGTC_2369|Compound:9071472|FD-Score:4.11|P-value:3.67E-4 ID:SGTC_2399|Compound:5884133|FD-Score:4.91|P-value:2.71E-5 ID:SGTC_2521|Compound:angolensin|FD-Score:3.99|P-value:5.24E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:6.17|P-value:1.95E-7 ID:SGTC_2688|Compound:kanamycin b|FD-Score:-4.70|P-value:5.39E-5 ID:SGTC_2862|Compound:9027634|FD-Score:4.63|P-value:7.16E-5 ID:SGTC_2872|Compound:9044037|FD-Score:3.88|P-value:7.24E-4 ID:SGTC_2936|Compound:9038235|FD-Score:3.91|P-value:6.53E-4 ID:SGTC_2942|Compound:9052777|FD-Score:4.43|P-value:1.38E-4 ID:SGTC_3006|Compound:9075424|FD-Score:-5.09|P-value:1.35E-5 ID:SGTC_3018|Compound:9082602|FD-Score:-5.05|P-value:1.56E-5 ID:SGTC_3052|Compound:9092717|FD-Score:4.29|P-value:2.11E-4 ID:SGTC_3219|Compound:9131112|FD-Score:-4.38|P-value:1.52E-4 ID:SGTC_3328|Compound:9140624|FD-Score:4.49|P-value:1.13E-4

Top fitness defect scores for YFR010W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_58 0957-0393 7.67 9.0 µM 1.43E-10 cell wall 136 8.61E-15
2 SGTC_159 3970-0959 7.02 50.0 µM 3.91E-9 17 1.10E-12
3 SGTC_2582 agaric acid 6.17 100.0 µM 1.95E-7 redox potentiating 150 3.36E-10
4 SGTC_2060 5238662 6.16 140.0 µM 2.03E-7 3 3.57E-10
5 SGTC_690 glyoxal 5.80 20.3 µM 9.20E-7 46 3.25E-9
6 SGTC_214 4373-3496 5.51 70.0 µM 3.00E-6 17 1.82E-8
7 SGTC_1661 st013063 5.16 9.7 µM 1.12E-5 12 1.24E-7
8 SGTC_1926 methyl fluorone black 5.12 53.6 µM 1.28E-5 DNA intercalators 33 1.52E-7
9 SGTC_2399 5884133 4.91 200.0 µM 2.71E-5 cell wall signaling 43 4.50E-7
10 SGTC_2009 4012297 4.75 12.1 µM 4.83E-5 cell wall signaling 41 1.04E-6
11 SGTC_2862 9027634 4.63 71.4 µM 7.16E-5 ERG2 20 1.85E-6
12 SGTC_2274 7947094 4.60 46.1 µM 7.84E-5 15 2.11E-6
13 SGTC_3328 9140624 4.49 29.0 µM 1.13E-4 9 3.57E-6
14 SGTC_2942 9052777 4.43 62.2 µM 1.38E-4 22 4.81E-6
15 SGTC_488 nitrendipine 4.38 139.0 µM 1.57E-4 36 5.80E-6
16 SGTC_851 0394-0702 4.36 453.0 µM 1.72E-4 mitochondrial processes 4 6.57E-6
17 SGTC_246 cycloheximide 4.35 667.0 µM 1.75E-4 10 6.75E-6
18 SGTC_818 4072-2707 4.30 53.5 µM 2.07E-4 9 8.66E-6
19 SGTC_3052 9092717 4.29 49.5 µM 2.11E-4 16 8.88E-6
20 SGTC_1722 st034304 4.20 3.6 µM 2.84E-4 mitochondrial processes 18 1.36E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.201 7.73E-32 YBR260C RGD1 hom GTPase-activating protein (RhoGAP) for Rho3p and Rho4p, possibly involved in control of actin cytoskeleton organization
0.200 1.34E-31 YKL213C DOA1 hom WD repeat protein required for ubiquitin-mediated protein degradation; forms a complex with Cdc48p; plays a role in controlling cellular ubiquitin concentration; also promotes efficient NHEJ in postdiauxic/stationary phase; protein increases in abundance and relocalizes from nucleus to nuclear periphery upon DNA replication stress
0.193 1.78E-29 YOR231W MKK1 hom MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk2p; MKK1 has a paralog, MKK2, that arose from the whole genome duplication
0.181 4.03E-26 YML103C NUP188 hom Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC organization and nucleocytoplasmic transport; homologous to human NUP188
0.171 1.61E-23 YLR412W BER1 hom Protein involved in microtubule-related processes, N-acetylation; GFP-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS; YLR412W is not an essential gene; similar to Arabidopsis SRR1 gene
0.170 3.07E-23 YDR049W VMS1 hom Component of a Cdc48p-complex involved in protein quality control; exhibits cytosolic and ER-membrane localization, with Cdc48p, during normal growth, and contributes to ER-associated degradation (ERAD) of specific substrates at a step after their ubiquitination; forms a mitochondrially-associated complex with Cdc48p and Npl4p under oxidative stress that is required for ubiquitin-mediated mitochondria-associated protein degradation (MAD); conserved in C. elegans and humans
0.165 5.89E-22 YIL160C POT1 hom 3-ketoacyl-CoA thiolase with broad chain length specificity, cleaves 3-ketoacyl-CoA into acyl-CoA and acetyl-CoA during beta-oxidation of fatty acids
0.164 1.05E-21 YGR196C FYV8 hom Protein of unknown function, required for survival upon exposure to K1 killer toxin
0.158 3.76E-20 YLR331C_d JIP3_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene MID2
0.152 7.57E-19 YNL291C MID1 hom N-glycosylated integral membrane protein of the ER membrane and plasma membrane, functions as a stretch-activated Ca2+-permeable cation channel required for Ca2+ influx stimulated by pheromone; interacts with Cch1p; forms an oligomer
0.151 1.40E-18 YDL162C_d YDL162C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps ENT1/YDL161W, a verified gene involved in endocytosis and actin cortical patch assembly
0.151 1.70E-18 YNL215W IES2 hom Protein that associates with the INO80 chromatin remodeling complex; associates with the INO80 complex under low-salt conditions; essential for growth under anaerobic conditions; protein abundance increases in response to DNA replication stress
0.143 1.05E-16 YLL039C UBI4 hom Ubiquitin; becomes conjugated to proteins, marking them for selective degradation via the ubiquitin-26S proteasome system; essential for the cellular stress response; encoded as a polyubiquitin precursor comprised of 5 head-to-tail repeats; protein abundance increases in response to DNA replication stress
0.141 1.97E-16 YGR217W CCH1 hom Voltage-gated high-affinity calcium channel involved in calcium influx in response to some environmental stresses as well as exposure to mating pheromones; interacts and co-localizes with Mid1p, suggesting Cch1p and Mid1p function together
0.141 2.18E-16 YIL111W COX5B hom Subunit Vb of cytochrome c oxidase; cytochrome c oxidase is the terminal member of the mitochondrial inner membrane electron transport chain; Cox5Bp is predominantly expressed during anaerobic growth while its isoform Va (Cox5Ap) is expressed during aerobic growth; COX5B has a paralog, COX5A, that arose from the whole genome duplication