YFR024C-A / LSB3

Protein containing a C-terminal SH3 domain; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; protein abundance increases in response to DNA replication stress; LSB3 has a paralog, YSC84, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YFR024C-A / LSB3

Click on Significant Values for Screen Details ID:SGTC_49|Compound:0717-0965|FD-Score:5.36|P-value:2.48E-8 ID:SGTC_51|Compound:1013-0126|FD-Score:-4.19|P-value:1.50E-5 ID:SGTC_56|Compound:0957-0365|FD-Score:3.76|P-value:6.28E-5 ID:SGTC_63|Compound:0099-0242|FD-Score:-4.76|P-value:9.88E-7 ID:SGTC_242|Compound:5hr heat shock (37°C) + 5-fluorouracil|FD-Score:3.94|P-value:2.96E-5 ID:SGTC_310|Compound:1273-0058|FD-Score:4.03|P-value:2.01E-5 ID:SGTC_489|Compound:paxilline|FD-Score:3.16|P-value:6.10E-4 ID:SGTC_490|Compound:phenamil|FD-Score:3.35|P-value:3.07E-4 ID:SGTC_665|Compound:3914-0008|FD-Score:-4.24|P-value:1.18E-5 ID:SGTC_741|Compound:4582-0002|FD-Score:4.75|P-value:6.69E-7 ID:SGTC_750|Compound:4073-0104|FD-Score:-3.32|P-value:4.87E-4 ID:SGTC_1033|Compound:k072-0102|FD-Score:4.87|P-value:3.54E-7 ID:SGTC_1047|Compound:2043-3044|FD-Score:3.17|P-value:5.88E-4 ID:SGTC_1089|Compound:terbinafine|FD-Score:3.47|P-value:1.98E-4 ID:SGTC_1094|Compound:duloxetine|FD-Score:3.50|P-value:1.72E-4 ID:SGTC_1112|Compound:tnp00069|FD-Score:3.50|P-value:1.76E-4 ID:SGTC_1239|Compound:0337-0584|FD-Score:3.76|P-value:6.09E-5 ID:SGTC_1246|Compound:0422-0085|FD-Score:3.19|P-value:5.55E-4 ID:SGTC_1532|Compound:4',5'-dihydroxyflavone|FD-Score:3.40|P-value:2.51E-4 ID:SGTC_1554|Compound:st069299|FD-Score:3.08|P-value:8.00E-4 ID:SGTC_1631|Compound:benzylvanillin|FD-Score:3.85|P-value:4.28E-5 ID:SGTC_1727|Compound:st034309|FD-Score:3.38|P-value:2.77E-4 ID:SGTC_1800|Compound:st050079|FD-Score:3.75|P-value:6.44E-5 ID:SGTC_1833|Compound:st055984|FD-Score:-4.05|P-value:2.67E-5 ID:SGTC_2023|Compound:5144124|FD-Score:-3.61|P-value:1.66E-4 ID:SGTC_2153|Compound:5569715|FD-Score:-4.13|P-value:1.90E-5 ID:SGTC_2155|Compound:5570423|FD-Score:-3.60|P-value:1.69E-4 ID:SGTC_2335|Compound:9018011|FD-Score:3.28|P-value:3.93E-4 ID:SGTC_2339|Compound:9000433|FD-Score:-3.44|P-value:3.10E-4 ID:SGTC_2383|Compound:9036125|FD-Score:-4.60|P-value:2.19E-6 ID:SGTC_2384|Compound:9036245|FD-Score:3.24|P-value:4.65E-4 ID:SGTC_2633|Compound:5,7-dihydroxyisoflavone|FD-Score:-3.49|P-value:2.57E-4 ID:SGTC_2691|Compound:st077356|FD-Score:3.63|P-value:1.05E-4 ID:SGTC_2825|Compound:7998094|FD-Score:3.06|P-value:8.58E-4 ID:SGTC_3028|Compound:9087778|FD-Score:-4.07|P-value:2.53E-5 ID:SGTC_3037|Compound:9091026|FD-Score:-4.23|P-value:1.25E-5 ID:SGTC_3191|Compound:9110352|FD-Score:-3.38|P-value:3.87E-4 ID:SGTC_3196|Compound:9111891|FD-Score:-4.20|P-value:1.39E-5 ID:SGTC_3224|Compound:9130643|FD-Score:-3.24|P-value:6.45E-4 ID:SGTC_3284|Compound:9108171|FD-Score:4.75|P-value:6.43E-7 ID:SGTC_3288|Compound:9119690|FD-Score:-3.19|P-value:7.61E-4 ID:SGTC_3290|Compound:9121031|FD-Score:4.73|P-value:7.25E-7 ID:SGTC_3301|Compound:9125354|FD-Score:3.10|P-value:7.44E-4 ID:SGTC_49|Compound:0717-0965|FD-Score:5.36|P-value:2.48E-8 ID:SGTC_51|Compound:1013-0126|FD-Score:-4.19|P-value:1.50E-5 ID:SGTC_56|Compound:0957-0365|FD-Score:3.76|P-value:6.28E-5 ID:SGTC_63|Compound:0099-0242|FD-Score:-4.76|P-value:9.88E-7 ID:SGTC_242|Compound:5hr heat shock (37°C) + 5-fluorouracil|FD-Score:3.94|P-value:2.96E-5 ID:SGTC_310|Compound:1273-0058|FD-Score:4.03|P-value:2.01E-5 ID:SGTC_489|Compound:paxilline|FD-Score:3.16|P-value:6.10E-4 ID:SGTC_490|Compound:phenamil|FD-Score:3.35|P-value:3.07E-4 ID:SGTC_665|Compound:3914-0008|FD-Score:-4.24|P-value:1.18E-5 ID:SGTC_741|Compound:4582-0002|FD-Score:4.75|P-value:6.69E-7 ID:SGTC_750|Compound:4073-0104|FD-Score:-3.32|P-value:4.87E-4 ID:SGTC_1033|Compound:k072-0102|FD-Score:4.87|P-value:3.54E-7 ID:SGTC_1047|Compound:2043-3044|FD-Score:3.17|P-value:5.88E-4 ID:SGTC_1089|Compound:terbinafine|FD-Score:3.47|P-value:1.98E-4 ID:SGTC_1094|Compound:duloxetine|FD-Score:3.50|P-value:1.72E-4 ID:SGTC_1112|Compound:tnp00069|FD-Score:3.50|P-value:1.76E-4 ID:SGTC_1239|Compound:0337-0584|FD-Score:3.76|P-value:6.09E-5 ID:SGTC_1246|Compound:0422-0085|FD-Score:3.19|P-value:5.55E-4 ID:SGTC_1532|Compound:4',5'-dihydroxyflavone|FD-Score:3.40|P-value:2.51E-4 ID:SGTC_1554|Compound:st069299|FD-Score:3.08|P-value:8.00E-4 ID:SGTC_1631|Compound:benzylvanillin|FD-Score:3.85|P-value:4.28E-5 ID:SGTC_1727|Compound:st034309|FD-Score:3.38|P-value:2.77E-4 ID:SGTC_1800|Compound:st050079|FD-Score:3.75|P-value:6.44E-5 ID:SGTC_1833|Compound:st055984|FD-Score:-4.05|P-value:2.67E-5 ID:SGTC_2023|Compound:5144124|FD-Score:-3.61|P-value:1.66E-4 ID:SGTC_2153|Compound:5569715|FD-Score:-4.13|P-value:1.90E-5 ID:SGTC_2155|Compound:5570423|FD-Score:-3.60|P-value:1.69E-4 ID:SGTC_2335|Compound:9018011|FD-Score:3.28|P-value:3.93E-4 ID:SGTC_2339|Compound:9000433|FD-Score:-3.44|P-value:3.10E-4 ID:SGTC_2383|Compound:9036125|FD-Score:-4.60|P-value:2.19E-6 ID:SGTC_2384|Compound:9036245|FD-Score:3.24|P-value:4.65E-4 ID:SGTC_2633|Compound:5,7-dihydroxyisoflavone|FD-Score:-3.49|P-value:2.57E-4 ID:SGTC_2691|Compound:st077356|FD-Score:3.63|P-value:1.05E-4 ID:SGTC_2825|Compound:7998094|FD-Score:3.06|P-value:8.58E-4 ID:SGTC_3028|Compound:9087778|FD-Score:-4.07|P-value:2.53E-5 ID:SGTC_3037|Compound:9091026|FD-Score:-4.23|P-value:1.25E-5 ID:SGTC_3191|Compound:9110352|FD-Score:-3.38|P-value:3.87E-4 ID:SGTC_3196|Compound:9111891|FD-Score:-4.20|P-value:1.39E-5 ID:SGTC_3224|Compound:9130643|FD-Score:-3.24|P-value:6.45E-4 ID:SGTC_3284|Compound:9108171|FD-Score:4.75|P-value:6.43E-7 ID:SGTC_3288|Compound:9119690|FD-Score:-3.19|P-value:7.61E-4 ID:SGTC_3290|Compound:9121031|FD-Score:4.73|P-value:7.25E-7 ID:SGTC_3301|Compound:9125354|FD-Score:3.10|P-value:7.44E-4

Top fitness defect scores for YFR024C-A deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_49 0717-0965 5.36 32.1 µM 2.48E-8 60S ribosome export 4 4.15E-8
2 SGTC_1033 k072-0102 4.87 21.3 µM 3.54E-7 60S ribosome export 6 5.54E-7
3 SGTC_3284 9108171 4.75 64.9 µM 6.43E-7 11 9.94E-7
4 SGTC_741 4582-0002 4.75 126.0 µM 6.69E-7 6 1.03E-6
5 SGTC_3290 9121031 4.73 71.4 µM 7.25E-7 3 1.12E-6
6 SGTC_310 1273-0058 4.03 1.1 µM 2.01E-5 mitochondrial stress 240 2.85E-5
7 SGTC_242 5hr heat shock (37°C) + 5-fluorouracil 3.94 4.6 µM 2.96E-5 heat shock/prefoldin 36 4.15E-5
8 SGTC_1631 benzylvanillin 3.85 81.9 µM 4.28E-5 Golgi 31 5.95E-5
9 SGTC_1239 0337-0584 3.76 35.4 µM 6.09E-5 unfolded protein response 64 8.38E-5
10 SGTC_56 0957-0365 3.76 83.2 µM 6.28E-5 Golgi 55 8.63E-5
11 SGTC_1800 st050079 3.75 29.1 µM 6.44E-5 NEO1-PIK1 131 8.84E-5
12 SGTC_2691 st077356 3.63 60.2 µM 1.05E-4 41 1.42E-4
13 SGTC_1094 duloxetine 3.50 25.4 µM 1.72E-4 RPP1 & pyrimidine depletion 33 2.30E-4
14 SGTC_1112 tnp00069 3.50 1.2 µM 1.76E-4 40 2.35E-4
15 SGTC_1089 terbinafine 3.47 2.2 µM 1.98E-4 30 2.64E-4
16 SGTC_1532 4',5'-dihydroxyflavone 3.40 78.7 µM 2.51E-4 Golgi 34 3.32E-4
17 SGTC_1727 st034309 3.38 9.9 µM 2.77E-4 71 3.65E-4
18 SGTC_490 phenamil 3.35 164.0 µM 3.07E-4 heme requiring 18 4.04E-4
19 SGTC_2335 9018011 3.28 154.2 µM 3.93E-4 51 5.14E-4
20 SGTC_2384 9036245 3.24 200.0 µM 4.65E-4 55 6.05E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.109 2.45E-10 YML130C ERO1 het Thiol oxidase required for oxidative protein folding in the ER; essential for maintaining proper redox balance in ER; feedback regulation of Ero1p occurs via reduction and oxidation of Ero1p regulatory bonds; reduced Pdi1p activates Ero1p by direct reduction of Ero1p regulatory bonds; depletion of thiol substrates and accumulation of oxidized Pdi1p results in inactivation of Ero1p by both Pdi1p-mediated oxidation and autonomous oxidation of Ero1p regulatory bonds
0.097 1.88E-8 YFL032W_d YFL032W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene HAC1/YFL031W; YFL032W is not an essential gene
0.096 2.40E-8 YLL062C MHT1 hom S-methylmethionine-homocysteine methyltransferase, functions along with Sam4p in the conversion of S-adenosylmethionine (AdoMet) to methionine to control the methionine/AdoMet ratio
0.093 7.49E-8 YFL052W_p YFL052W_p hom Putative zinc cluster protein that contains a DNA binding domain; computational analysis suggests a role as a transcription factor; null mutant is sensitive to Calcofluor White, low osmolarity, and heat, suggesting a role for YFL052Wp in cell wall integrity
0.087 4.96E-7 YPR178W PRP4 het Splicing factor, component of the U4/U6-U5 snRNP complex
0.085 7.73E-7 YOR292C_p YOR292C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; YOR292C is not an essential gene
0.082 1.99E-6 YJL212C OPT1 hom Proton-coupled oligopeptide transporter of the plasma membrane; also transports glutathione and phytochelatin; member of the OPT family
0.081 2.98E-6 YDR534C FIT1 hom Mannoprotein that is incorporated into the cell wall via a glycosylphosphatidylinositol (GPI) anchor, involved in the retention of siderophore-iron in the cell wall
0.080 3.64E-6 YML116W-A_d YML116W-A_d hom Putative protein of unknown function
0.078 6.50E-6 YLR024C UBR2 hom Cytoplasmic ubiquitin-protein ligase (E3); required for ubiquitylation of Rpn4p; mediates formation of a Mub1p-Ubr2p-Rad6p complex
0.076 9.66E-6 YGR079W_p YGR079W_p hom Putative protein of unknown function; YGR079W is not an essential gene
0.075 1.25E-5 YMR139W RIM11 hom Protein kinase; required for signal transduction during entry into meiosis; promotes the formation of the Ime1p-Ume6p complex by phosphorylating Ime1p and Ume6p; shares similarity with mammalian glycogen synthase kinase 3-beta; protein abundance increases in response to DNA replication stress
0.075 1.48E-5 YDR116C MRPL1 hom Mitochondrial ribosomal protein of the large subunit
0.072 3.08E-5 YPL202C AFT2 hom Iron-regulated transcriptional activator; activates genes involved in intracellular iron use and required for iron homeostasis and resistance to oxidative stress; AFT2 has a paralog, AFT1, that arose from the whole genome duplication
0.070 4.80E-5 YBR042C CST26 hom Putative transferase involved in phospholipid biosynthesis; required for incorporation of stearic acid into phosphatidylinositol; affects chromosome stability when overexpressed; CST26 has a paralog, YDR018C, that arose from the whole genome duplication