YFR028C / CDC14

Protein phosphatase required for mitotic exit; located in the nucleolus until liberated by the FEAR and Mitotic Exit Network in anaphase, enabling it to act on key substrates to effect a decrease in CDK/B-cyclin activity and mitotic exit; required for meiosis I spindle disassembly; released from nucleolus upon entry into anaphase I of meiosis, resequestered in metaphase II, then released again upon entry into anaphase II; maintained in nucleolus by Cdc55p in early meiosis

Zygosity: Heterozygous strain
fixedexpanded
Profile for YFR028C / CDC14

Click on Significant Values for Screen Details ID:SGTC_73|Compound:0327-0325|FD-Score:-3.31|P-value:3.69E-4 ID:SGTC_158|Compound:k038-0087|FD-Score:3.03|P-value:8.21E-4 ID:SGTC_188|Compound:3229-0966|FD-Score:3.19|P-value:4.62E-4 ID:SGTC_237|Compound:nikkomycin|FD-Score:4.42|P-value:2.32E-6 ID:SGTC_243|Compound:5hr heat shock (37°C) + splitomicin|FD-Score:3.59|P-value:9.74E-5 ID:SGTC_479|Compound:n-linoleoylglycine|FD-Score:3.36|P-value:2.39E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:3.36|P-value:2.38E-4 ID:SGTC_916|Compound:4466-0038|FD-Score:3.19|P-value:4.63E-4 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:4.28|P-value:4.62E-6 ID:SGTC_1155|Compound:4088-0394|FD-Score:4.32|P-value:3.75E-6 ID:SGTC_1462|Compound:k081-0014|FD-Score:-4.30|P-value:5.65E-6 ID:SGTC_1463|Compound:k081-0032|FD-Score:-4.54|P-value:1.82E-6 ID:SGTC_1725|Compound:st036284|FD-Score:3.63|P-value:8.20E-5 ID:SGTC_1885|Compound:st057546|FD-Score:-3.08|P-value:8.54E-4 ID:SGTC_1914|Compound:st062367|FD-Score:3.06|P-value:7.33E-4 ID:SGTC_1953|Compound:st076800|FD-Score:4.82|P-value:2.97E-7 ID:SGTC_2242|Compound:6630449|FD-Score:3.76|P-value:4.79E-5 ID:SGTC_2322|Compound:7413319|FD-Score:-3.17|P-value:6.23E-4 ID:SGTC_2394|Compound:5221648|FD-Score:4.22|P-value:6.22E-6 ID:SGTC_2396|Compound:5342950|FD-Score:-3.22|P-value:5.27E-4 ID:SGTC_2399|Compound:5884133|FD-Score:-4.43|P-value:3.08E-6 ID:SGTC_2404|Compound:5161160|FD-Score:4.65|P-value:7.20E-7 ID:SGTC_2488|Compound:5268135|FD-Score:-3.11|P-value:7.72E-4 ID:SGTC_2509|Compound:methyl 7-deshydroxypyrogallin-4-carboxylate|FD-Score:-3.13|P-value:7.21E-4 ID:SGTC_2556|Compound:citropten|FD-Score:-3.17|P-value:6.23E-4 ID:SGTC_2614|Compound:arthonioic acid|FD-Score:3.04|P-value:7.91E-4 ID:SGTC_2619|Compound:obliquin|FD-Score:4.94|P-value:1.61E-7 ID:SGTC_2729|Compound:fluphenazine|FD-Score:-3.91|P-value:3.35E-5 ID:SGTC_2800|Compound:7887935|FD-Score:3.26|P-value:3.58E-4 ID:SGTC_2853|Compound:9015042|FD-Score:-3.72|P-value:7.31E-5 ID:SGTC_2856|Compound:9022925|FD-Score:3.35|P-value:2.49E-4 ID:SGTC_2942|Compound:9052777|FD-Score:3.24|P-value:3.76E-4 ID:SGTC_2946|Compound:9065502|FD-Score:-4.10|P-value:1.43E-5 ID:SGTC_3157|Compound:9099274|FD-Score:-4.37|P-value:4.04E-6 ID:SGTC_3193|Compound:9111931|FD-Score:4.38|P-value:2.90E-6 ID:SGTC_3198|Compound:9112019|FD-Score:3.80|P-value:4.03E-5 ID:SGTC_3199|Compound:9111318|FD-Score:-4.88|P-value:3.14E-7 ID:SGTC_3305|Compound:9127027|FD-Score:-3.38|P-value:2.90E-4 ID:SGTC_73|Compound:0327-0325|FD-Score:-3.31|P-value:3.69E-4 ID:SGTC_158|Compound:k038-0087|FD-Score:3.03|P-value:8.21E-4 ID:SGTC_188|Compound:3229-0966|FD-Score:3.19|P-value:4.62E-4 ID:SGTC_237|Compound:nikkomycin|FD-Score:4.42|P-value:2.32E-6 ID:SGTC_243|Compound:5hr heat shock (37°C) + splitomicin|FD-Score:3.59|P-value:9.74E-5 ID:SGTC_479|Compound:n-linoleoylglycine|FD-Score:3.36|P-value:2.39E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:3.36|P-value:2.38E-4 ID:SGTC_916|Compound:4466-0038|FD-Score:3.19|P-value:4.63E-4 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:4.28|P-value:4.62E-6 ID:SGTC_1155|Compound:4088-0394|FD-Score:4.32|P-value:3.75E-6 ID:SGTC_1462|Compound:k081-0014|FD-Score:-4.30|P-value:5.65E-6 ID:SGTC_1463|Compound:k081-0032|FD-Score:-4.54|P-value:1.82E-6 ID:SGTC_1725|Compound:st036284|FD-Score:3.63|P-value:8.20E-5 ID:SGTC_1885|Compound:st057546|FD-Score:-3.08|P-value:8.54E-4 ID:SGTC_1914|Compound:st062367|FD-Score:3.06|P-value:7.33E-4 ID:SGTC_1953|Compound:st076800|FD-Score:4.82|P-value:2.97E-7 ID:SGTC_2242|Compound:6630449|FD-Score:3.76|P-value:4.79E-5 ID:SGTC_2322|Compound:7413319|FD-Score:-3.17|P-value:6.23E-4 ID:SGTC_2394|Compound:5221648|FD-Score:4.22|P-value:6.22E-6 ID:SGTC_2396|Compound:5342950|FD-Score:-3.22|P-value:5.27E-4 ID:SGTC_2399|Compound:5884133|FD-Score:-4.43|P-value:3.08E-6 ID:SGTC_2404|Compound:5161160|FD-Score:4.65|P-value:7.20E-7 ID:SGTC_2488|Compound:5268135|FD-Score:-3.11|P-value:7.72E-4 ID:SGTC_2509|Compound:methyl 7-deshydroxypyrogallin-4-carboxylate|FD-Score:-3.13|P-value:7.21E-4 ID:SGTC_2556|Compound:citropten|FD-Score:-3.17|P-value:6.23E-4 ID:SGTC_2614|Compound:arthonioic acid|FD-Score:3.04|P-value:7.91E-4 ID:SGTC_2619|Compound:obliquin|FD-Score:4.94|P-value:1.61E-7 ID:SGTC_2729|Compound:fluphenazine|FD-Score:-3.91|P-value:3.35E-5 ID:SGTC_2800|Compound:7887935|FD-Score:3.26|P-value:3.58E-4 ID:SGTC_2853|Compound:9015042|FD-Score:-3.72|P-value:7.31E-5 ID:SGTC_2856|Compound:9022925|FD-Score:3.35|P-value:2.49E-4 ID:SGTC_2942|Compound:9052777|FD-Score:3.24|P-value:3.76E-4 ID:SGTC_2946|Compound:9065502|FD-Score:-4.10|P-value:1.43E-5 ID:SGTC_3157|Compound:9099274|FD-Score:-4.37|P-value:4.04E-6 ID:SGTC_3193|Compound:9111931|FD-Score:4.38|P-value:2.90E-6 ID:SGTC_3198|Compound:9112019|FD-Score:3.80|P-value:4.03E-5 ID:SGTC_3199|Compound:9111318|FD-Score:-4.88|P-value:3.14E-7 ID:SGTC_3305|Compound:9127027|FD-Score:-3.38|P-value:2.90E-4

Top fitness defect scores for YFR028C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature		Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2619 obliquin 4.94 87.9 µM 1.61E-7 cell wall 26 3.98E-7 0.29
2 SGTC_1953 st076800 4.82 12.1 µM 2.97E-7 5 7.06E-7 0.21
3 SGTC_2404 5161160 4.65 200.0 µM 7.20E-7 5 1.63E-6 0.14
4 SGTC_237 nikkomycin 4.42 200.0 µM 2.32E-6 RSC complex & mRNA processing 6 4.90E-6 0.04
5 SGTC_3193 9111931 4.38 49.5 µM 2.90E-6 5 6.04E-6 0.27
6 SGTC_1155 4088-0394 4.32 48.2 µM 3.75E-6 2 7.69E-6 0.25
7 SGTC_1083 didemethylchlorpromazine sulfoxide 4.28 707.4 nM 4.62E-6 redox potentiating 22 9.37E-6 0.31
8 SGTC_2394 5221648 4.22 200.0 µM 6.22E-6 6 1.24E-5 0.49
9 SGTC_3198 9112019 3.80 49.5 µM 4.03E-5 mitochondrial processes 6 7.20E-5 0.25
10 SGTC_2242 6630449 3.76 61.3 µM 4.79E-5 Golgi 6 8.46E-5 0.35
11 SGTC_1725 st036284 3.63 20.6 µM 8.20E-5 6 1.40E-4 0.09
12 SGTC_243 5hr heat shock (37°C) + splitomicin 3.59 1.0 µM 9.74E-5 heat shock/prefoldin 6 1.65E-4 0.14
13 SGTC_514 4466-0038 3.36 2.6 nM 2.38E-4 cell wall 50 3.83E-4 0.09
14 SGTC_479 n-linoleoylglycine 3.36 10.0 µM 2.39E-4 2 3.84E-4 0.01
15 SGTC_2856 9022925 3.35 19.5 µM 2.49E-4 13 4.00E-4 0.05
16 SGTC_2800 7887935 3.26 81.8 µM 3.58E-4 RPP1 & pyrimidine depletion 16 5.61E-4 0.05
17 SGTC_2942 9052777 3.24 62.2 µM 3.76E-4 17 5.88E-4 0.02
18 SGTC_188 3229-0966 3.19 278.5 µM 4.62E-4 32 7.13E-4 0.01
19 SGTC_916 4466-0038 3.19 42.0 nM 4.63E-4 cell wall 35 7.15E-4 0.03
20 SGTC_1914 st062367 3.06 72.6 µM 7.33E-4 11 0.00110 0.02

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.243 2.74E-46 YJL159W HSP150 hom O-mannosylated heat shock protein; secreted and covalently attached to the cell wall via beta-1,3-glucan and disulfide bridges; required for cell wall stability; induced by heat shock, oxidative stress, and nitrogen limitation; HSP150 has a paralog, PIR3, that arose from the whole genome duplication
0.225 1.51E-39 YKR102W FLO10 hom Member of the FLO family of cell wall flocculation proteins; not expressed in most lab strains; overproduction induces flocculation that can be inhibited by mannose, sucrose, or glucose; overproduction also promotes haploid invasive growth and diploid filamentous growth
0.195 7.52E-30 YDR521W_d YDR521W_d hom Dubious ORF that overlaps YDR520C; mutant increases expression of PIS1 and RPL3 in glycerol
0.189 2.40E-28 YJL171C YJL171C hom GPI-anchored cell wall protein of unknown function; induced in response to cell wall damaging agents and by mutations in genes involved in cell wall biogenesis; sequence similarity to YBR162C/TOS1, a covalently bound cell wall protein; protein abundance increases in response to DNA replication stress
0.186 2.26E-27 YGR022C_d YGR022C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF MTL1/YGR023W
0.184 9.79E-27 YOR297C TIM18 hom Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane; may mediate assembly or stability of the complex
0.179 2.02E-25 YNL166C BNI5 hom Protein involved in organization of septins at the mother-bud neck, may interact directly with the Cdc11p septin, localizes to bud neck in a septin-dependent manner
0.176 1.48E-24 YPL251W_d YPL251W_d het Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified, essential gene YAH1/YPL252C
0.175 2.97E-24 YPR180W AOS1 het Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with Uba2p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability
0.160 1.18E-20 YGR077C PEX8 hom Intraperoxisomal organizer of the peroxisomal import machinery; organizes the formation of the importomer complex, bridging the docking complex with the RING finger complex; tightly associated with the lumenal face of the peroxisomal membrane; essential for peroxisome biogenesis; binds PTS1-signal receptor Pex5p, and PTS2-signal receptor Pex7p
0.160 1.53E-20 YBR274W CHK1 hom Serine/threonine kinase and DNA damage checkpoint effector, mediates cell cycle arrest via phosphorylation of Pds1p; phosphorylated by checkpoint signal transducer Mec1p; homolog of S. pombe and mammalian Chk1 checkpoint kinase
0.159 2.56E-20 YDL157C_p YDL157C_p hom Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.159 2.60E-20 YMR121C RPL15B hom Ribosomal 60S subunit protein L15B; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15B has a paralog, RPL15A, that arose from the whole genome duplication; relocalizes from nucleus to nucleolus upon DNA replication stress
0.155 2.53E-19 YMR124W_p YMR124W_p hom Protein of unknown function; GFP-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; interacts with Crm1p in two-hybrid assay; YMR124W is not an essential gene; predicted to have a role in organelle organization
0.154 3.98E-19 YDL227C HO hom Site-specific endonuclease required for gene conversion at the MAT locus (homothallic switching) through the generation of a ds DNA break; expression restricted to mother cells in late G1 as controlled by Swi4p-Swi6p, Swi5p and Ash1p