YFR032C_p / RRT5

Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; expressed at high levels during sporulation

Zygosity: Homozygous strain
fixedexpanded
Profile for YFR032C / RRT5

Click on Significant Values for Screen Details ID:SGTC_123|Compound:0083-0120|FD-Score:3.04|P-value:9.60E-4 ID:SGTC_160|Compound:3453-0671|FD-Score:3.90|P-value:3.37E-5 ID:SGTC_219|Compound:mitoxanthrone|FD-Score:8.21|P-value:2.49E-17 ID:SGTC_568|Compound:1193-0097|FD-Score:4.09|P-value:1.50E-5 ID:SGTC_653|Compound:0203-0016|FD-Score:3.94|P-value:2.82E-5 ID:SGTC_685|Compound:1278-0033|FD-Score:5.02|P-value:1.44E-7 ID:SGTC_691|Compound:1548-0686|FD-Score:4.30|P-value:5.53E-6 ID:SGTC_705|Compound:1124-0212|FD-Score:3.38|P-value:2.79E-4 ID:SGTC_841|Compound:0251-0215|FD-Score:3.16|P-value:6.30E-4 ID:SGTC_888|Compound:0916-0002|FD-Score:-3.19|P-value:5.64E-4 ID:SGTC_941|Compound:3237-0776|FD-Score:6.63|P-value:6.34E-12 ID:SGTC_946|Compound:3434-1334|FD-Score:3.71|P-value:7.62E-5 ID:SGTC_1158|Compound:4092-0494|FD-Score:3.50|P-value:1.76E-4 ID:SGTC_1180|Compound:1193-0155|FD-Score:3.92|P-value:3.06E-5 ID:SGTC_1254|Compound:0550-0035|FD-Score:3.17|P-value:6.02E-4 ID:SGTC_1261|Compound:0712-0010|FD-Score:4.76|P-value:5.93E-7 ID:SGTC_1400|Compound:3771-8998|FD-Score:3.03|P-value:9.74E-4 ID:SGTC_1484|Compound:3473-2379|FD-Score:3.38|P-value:2.76E-4 ID:SGTC_1524|Compound:corynanthine|FD-Score:3.56|P-value:1.38E-4 ID:SGTC_1550|Compound:gossypolone|FD-Score:3.13|P-value:6.90E-4 ID:SGTC_1595|Compound:6-ethoxy-3(4'-hydroxyphenyl)-4-methylcoumarin|FD-Score:3.29|P-value:3.83E-4 ID:SGTC_1629|Compound:st005085|FD-Score:4.16|P-value:1.06E-5 ID:SGTC_1632|Compound:st005650|FD-Score:6.29|P-value:6.50E-11 ID:SGTC_1634|Compound:st006576|FD-Score:3.36|P-value:3.00E-4 ID:SGTC_1638|Compound:st008370|FD-Score:4.58|P-value:1.43E-6 ID:SGTC_1738|Compound:st035555|FD-Score:-8.34|P-value:7.94E-18 ID:SGTC_1742|Compound:st038235|FD-Score:4.50|P-value:2.12E-6 ID:SGTC_1750|Compound:st081859|FD-Score:4.02|P-value:2.04E-5 ID:SGTC_1778|Compound:st046819|FD-Score:4.46|P-value:2.65E-6 ID:SGTC_1887|Compound:st057648|FD-Score:3.31|P-value:3.61E-4 ID:SGTC_1901|Compound:st060216|FD-Score:-4.20|P-value:8.80E-6 ID:SGTC_1902|Compound:st060212|FD-Score:-3.20|P-value:5.38E-4 ID:SGTC_1906|Compound:st060206|FD-Score:3.84|P-value:4.43E-5 ID:SGTC_1932|Compound:st074806|FD-Score:3.19|P-value:5.64E-4 ID:SGTC_1940|Compound:st075990|FD-Score:3.03|P-value:9.78E-4 ID:SGTC_1989|Compound:st071868|FD-Score:3.28|P-value:4.04E-4 ID:SGTC_2185|Compound:6113692|FD-Score:3.40|P-value:2.61E-4 ID:SGTC_2258|Compound:7960471|FD-Score:4.14|P-value:1.17E-5 ID:SGTC_2319|Compound:6033012|FD-Score:-3.03|P-value:9.70E-4 ID:SGTC_2345|Compound:9052315|FD-Score:3.64|P-value:1.01E-4 ID:SGTC_2422|Compound:chlorphenesin carbamate|FD-Score:-3.62|P-value:1.08E-4 ID:SGTC_2553|Compound:4-methylesculetin|FD-Score:-3.40|P-value:2.58E-4 ID:SGTC_2616|Compound:abietic acid|FD-Score:3.19|P-value:5.61E-4 ID:SGTC_2694|Compound:st077685|FD-Score:3.77|P-value:5.78E-5 ID:SGTC_2698|Compound:st077697|FD-Score:3.39|P-value:2.71E-4 ID:SGTC_2712|Compound:st079126|FD-Score:3.26|P-value:4.35E-4 ID:SGTC_2890|Compound:9046729|FD-Score:3.74|P-value:6.60E-5 ID:SGTC_2907|Compound:7721985|FD-Score:3.31|P-value:3.57E-4 ID:SGTC_3052|Compound:9092717|FD-Score:5.15|P-value:7.32E-8 ID:SGTC_3056|Compound:9094900|FD-Score:-3.28|P-value:4.01E-4 ID:SGTC_3071|Compound:9117417|FD-Score:3.48|P-value:1.87E-4 ID:SGTC_3116|Compound:9124943|FD-Score:4.15|P-value:1.12E-5 ID:SGTC_3211|Compound:9128781|FD-Score:-3.76|P-value:5.93E-5 ID:SGTC_3212|Compound:9128307|FD-Score:6.11|P-value:2.09E-10 ID:SGTC_3215|Compound:9129942|FD-Score:3.10|P-value:7.81E-4 ID:SGTC_3240|Compound:9133527|FD-Score:3.99|P-value:2.27E-5 ID:SGTC_3324|Compound:9138759|FD-Score:-3.69|P-value:8.24E-5 ID:SGTC_123|Compound:0083-0120|FD-Score:3.04|P-value:9.60E-4 ID:SGTC_160|Compound:3453-0671|FD-Score:3.90|P-value:3.37E-5 ID:SGTC_219|Compound:mitoxanthrone|FD-Score:8.21|P-value:2.49E-17 ID:SGTC_568|Compound:1193-0097|FD-Score:4.09|P-value:1.50E-5 ID:SGTC_653|Compound:0203-0016|FD-Score:3.94|P-value:2.82E-5 ID:SGTC_685|Compound:1278-0033|FD-Score:5.02|P-value:1.44E-7 ID:SGTC_691|Compound:1548-0686|FD-Score:4.30|P-value:5.53E-6 ID:SGTC_705|Compound:1124-0212|FD-Score:3.38|P-value:2.79E-4 ID:SGTC_841|Compound:0251-0215|FD-Score:3.16|P-value:6.30E-4 ID:SGTC_888|Compound:0916-0002|FD-Score:-3.19|P-value:5.64E-4 ID:SGTC_941|Compound:3237-0776|FD-Score:6.63|P-value:6.34E-12 ID:SGTC_946|Compound:3434-1334|FD-Score:3.71|P-value:7.62E-5 ID:SGTC_1158|Compound:4092-0494|FD-Score:3.50|P-value:1.76E-4 ID:SGTC_1180|Compound:1193-0155|FD-Score:3.92|P-value:3.06E-5 ID:SGTC_1254|Compound:0550-0035|FD-Score:3.17|P-value:6.02E-4 ID:SGTC_1261|Compound:0712-0010|FD-Score:4.76|P-value:5.93E-7 ID:SGTC_1400|Compound:3771-8998|FD-Score:3.03|P-value:9.74E-4 ID:SGTC_1484|Compound:3473-2379|FD-Score:3.38|P-value:2.76E-4 ID:SGTC_1524|Compound:corynanthine|FD-Score:3.56|P-value:1.38E-4 ID:SGTC_1550|Compound:gossypolone|FD-Score:3.13|P-value:6.90E-4 ID:SGTC_1595|Compound:6-ethoxy-3(4'-hydroxyphenyl)-4-methylcoumarin|FD-Score:3.29|P-value:3.83E-4 ID:SGTC_1629|Compound:st005085|FD-Score:4.16|P-value:1.06E-5 ID:SGTC_1632|Compound:st005650|FD-Score:6.29|P-value:6.50E-11 ID:SGTC_1634|Compound:st006576|FD-Score:3.36|P-value:3.00E-4 ID:SGTC_1638|Compound:st008370|FD-Score:4.58|P-value:1.43E-6 ID:SGTC_1738|Compound:st035555|FD-Score:-8.34|P-value:7.94E-18 ID:SGTC_1742|Compound:st038235|FD-Score:4.50|P-value:2.12E-6 ID:SGTC_1750|Compound:st081859|FD-Score:4.02|P-value:2.04E-5 ID:SGTC_1778|Compound:st046819|FD-Score:4.46|P-value:2.65E-6 ID:SGTC_1887|Compound:st057648|FD-Score:3.31|P-value:3.61E-4 ID:SGTC_1901|Compound:st060216|FD-Score:-4.20|P-value:8.80E-6 ID:SGTC_1902|Compound:st060212|FD-Score:-3.20|P-value:5.38E-4 ID:SGTC_1906|Compound:st060206|FD-Score:3.84|P-value:4.43E-5 ID:SGTC_1932|Compound:st074806|FD-Score:3.19|P-value:5.64E-4 ID:SGTC_1940|Compound:st075990|FD-Score:3.03|P-value:9.78E-4 ID:SGTC_1989|Compound:st071868|FD-Score:3.28|P-value:4.04E-4 ID:SGTC_2185|Compound:6113692|FD-Score:3.40|P-value:2.61E-4 ID:SGTC_2258|Compound:7960471|FD-Score:4.14|P-value:1.17E-5 ID:SGTC_2319|Compound:6033012|FD-Score:-3.03|P-value:9.70E-4 ID:SGTC_2345|Compound:9052315|FD-Score:3.64|P-value:1.01E-4 ID:SGTC_2422|Compound:chlorphenesin carbamate|FD-Score:-3.62|P-value:1.08E-4 ID:SGTC_2553|Compound:4-methylesculetin|FD-Score:-3.40|P-value:2.58E-4 ID:SGTC_2616|Compound:abietic acid|FD-Score:3.19|P-value:5.61E-4 ID:SGTC_2694|Compound:st077685|FD-Score:3.77|P-value:5.78E-5 ID:SGTC_2698|Compound:st077697|FD-Score:3.39|P-value:2.71E-4 ID:SGTC_2712|Compound:st079126|FD-Score:3.26|P-value:4.35E-4 ID:SGTC_2890|Compound:9046729|FD-Score:3.74|P-value:6.60E-5 ID:SGTC_2907|Compound:7721985|FD-Score:3.31|P-value:3.57E-4 ID:SGTC_3052|Compound:9092717|FD-Score:5.15|P-value:7.32E-8 ID:SGTC_3056|Compound:9094900|FD-Score:-3.28|P-value:4.01E-4 ID:SGTC_3071|Compound:9117417|FD-Score:3.48|P-value:1.87E-4 ID:SGTC_3116|Compound:9124943|FD-Score:4.15|P-value:1.12E-5 ID:SGTC_3211|Compound:9128781|FD-Score:-3.76|P-value:5.93E-5 ID:SGTC_3212|Compound:9128307|FD-Score:6.11|P-value:2.09E-10 ID:SGTC_3215|Compound:9129942|FD-Score:3.10|P-value:7.81E-4 ID:SGTC_3240|Compound:9133527|FD-Score:3.99|P-value:2.27E-5 ID:SGTC_3324|Compound:9138759|FD-Score:-3.69|P-value:8.24E-5

Top fitness defect scores for YFR032C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_219 mitoxanthrone 8.21 164.4 µM 2.49E-17 DNA intercalators 14 1.11E-16
2 SGTC_941 3237-0776 6.63 460.0 µM 6.34E-12 1 1.69E-11
3 SGTC_1632 st005650 6.29 90.0 µM 6.50E-11 2 1.58E-10
4 SGTC_3212 9128307 6.11 49.5 µM 2.09E-10 14 4.84E-10
5 SGTC_3052 9092717 5.15 49.5 µM 7.32E-8 6 1.33E-7
6 SGTC_685 1278-0033 5.02 54.9 µM 1.44E-7 60S ribosome export 7 2.54E-7
7 SGTC_1261 0712-0010 4.76 12.7 µM 5.93E-7 RNA processing & uracil transport 12 9.89E-7
8 SGTC_1638 st008370 4.58 60.6 µM 1.43E-6 ergosterol biosynthesis 18 2.30E-6
9 SGTC_1742 st038235 4.50 28.9 µM 2.12E-6 mitochondrial stress 97 3.36E-6
10 SGTC_1778 st046819 4.46 58.9 µM 2.65E-6 16 4.16E-6
11 SGTC_691 1548-0686 4.30 185.0 µM 5.53E-6 22 8.44E-6
12 SGTC_1629 st005085 4.16 44.9 µM 1.06E-5 RPP1 & pyrimidine depletion 30 1.58E-5
13 SGTC_3116 9124943 4.15 49.5 µM 1.12E-5 11 1.66E-5
14 SGTC_2258 7960471 4.14 200.0 µM 1.17E-5 7 1.73E-5
15 SGTC_568 1193-0097 4.09 7.5 µM 1.50E-5 ERAD & cell cycle 139 2.19E-5
16 SGTC_1750 st081859 4.02 2.4 µM 2.04E-5 endomembrane recycling 181 2.95E-5
17 SGTC_3240 9133527 3.99 49.5 µM 2.27E-5 11 3.26E-5
18 SGTC_653 0203-0016 3.94 55.2 µM 2.82E-5 15 4.03E-5
19 SGTC_1180 1193-0155 3.92 285.0 µM 3.06E-5 14 4.36E-5
20 SGTC_160 3453-0671 3.90 289.7 µM 3.37E-5 40 4.77E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.143 8.33E-17 YMR126C_p DLT1_p hom Protein of unknown function, mutant sensitive to 6-azauracil (6AU) and mycophenolic acid (MPA)
0.114 3.43E-11 YPR153W YPR153W hom Putative protein of unknown function
0.108 3.68E-10 YJR057W CDC8 het Thymidylate and uridylate kinase, functions in de novo biosynthesis of pyrimidine deoxyribonucleotides; converts dTMP to dTDP and dUMP to dUTP; essential for mitotic and meiotic DNA replication; homologous to S. pombe Tmp1p
0.107 5.27E-10 YHL003C LAG1 hom Ceramide synthase component; involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lac1p; forms ER foci upon DNA replication stress
0.103 1.98E-9 YPL077C_p YPL077C_p hom Putative protein of unknown function; regulates PIS1 expression; mutant displays spore wall assembly defect in ether sensitivity screen; YPL077C is not an essential gene; YPL077C has a paralog, YBR197C, that arose from the whole genome duplication
0.102 3.34E-9 YPL167C REV3 hom Catalytic subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair
0.099 1.02E-8 YML072C TCB3 hom Cortical ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; localized to the bud via specific mRNA transport; non-tagged protein detected in a phosphorylated state in mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact
0.096 2.34E-8 YCR068W ATG15 hom Lipase required for intravacuolar lysis of autophagic bodies and Cvt bodies; targeted to intravacuolar vesicles during autophagy via the multivesicular body (MVB) pathway
0.096 2.76E-8 YKL083W_d YKL083W_d het Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified essential gene RRP14
0.095 4.11E-8 YPL262W FUM1 hom Fumarase, converts fumaric acid to L-malic acid in the TCA cycle; cytosolic and mitochondrial distribution determined by the N-terminal targeting sequence, protein conformation, and status of glyoxylate shunt; phosphorylated in mitochondria
0.094 4.66E-8 YKL098W MTC2 hom Protein of unknown function; mtc2 is synthetically sick with cdc13-1
0.094 5.60E-8 YKL084W HOT13 hom Zinc-binding mitochondrial intermembrane space (IMS) protein involved in a disulfide relay system for IMS import of cysteine-containing proteins; binds Mia40p and stimulates its Erv1p-dependent oxidation, probably by sequestering zinc
0.093 5.77E-8 YHL039W EFM1 hom Lysine methyltransferase involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.092 8.07E-8 YDR082W STN1 het Telomere end-binding and capping protein, plays a key role with Pol12p in linking telomerase action with completion of lagging strand synthesis, and in a regulatory step required for telomere capping
0.092 9.64E-8 YMR168C CEP3 het Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain