YFR034C / PHO4

Basic helix-loop-helix (bHLH) transcription factor of the myc-family; activates transcription cooperatively with Pho2p in response to phosphate limitation; binding to 'CACGTG' motif is regulated by chromatin restriction, competitive binding of Cbf1p to the same DNA binding motif and cooperation with Pho2p,; function is regulated by phosphorylation at multiple sites and by phosphate availability

Zygosity: Homozygous strain
fixedexpanded
Profile for YFR034C / PHO4

Click on Significant Values for Screen Details ID:SGTC_116|Compound:0155-0182|FD-Score:-3.89|P-value:3.42E-4 ID:SGTC_118|Compound:0083-0097|FD-Score:5.24|P-value:2.72E-6 ID:SGTC_146|Compound:3448-8650|FD-Score:6.76|P-value:2.27E-9 ID:SGTC_151|Compound:3013-0144|FD-Score:-3.87|P-value:3.62E-4 ID:SGTC_253|Compound:dyclonine|FD-Score:4.58|P-value:3.53E-5 ID:SGTC_276|Compound:colchicine|FD-Score:4.07|P-value:2.13E-4 ID:SGTC_299|Compound:1273-0059|FD-Score:-3.70|P-value:6.08E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:-4.24|P-value:1.06E-4 ID:SGTC_346|Compound:4513-0042|FD-Score:-5.04|P-value:5.30E-6 ID:SGTC_351|Compound:caspofungin|FD-Score:3.59|P-value:9.37E-4 ID:SGTC_356|Compound:1634-0012|FD-Score:4.34|P-value:8.39E-5 ID:SGTC_360|Compound:0957-0399|FD-Score:4.52|P-value:4.43E-5 ID:SGTC_375|Compound:3970-0961|FD-Score:3.69|P-value:7.00E-4 ID:SGTC_464|Compound:bw-b 70c|FD-Score:3.82|P-value:4.64E-4 ID:SGTC_469|Compound:w7|FD-Score:6.82|P-value:1.58E-9 ID:SGTC_474|Compound:dl-dihydrosphingosine|FD-Score:5.08|P-value:5.31E-6 ID:SGTC_513|Compound:4245-1575|FD-Score:3.60|P-value:9.03E-4 ID:SGTC_679|Compound:0422-0091|FD-Score:3.73|P-value:6.11E-4 ID:SGTC_810|Compound:3825-7150|FD-Score:-4.11|P-value:1.63E-4 ID:SGTC_832|Compound:4083-0019|FD-Score:4.31|P-value:9.52E-5 ID:SGTC_878|Compound:0828-0289|FD-Score:4.83|P-value:1.42E-5 ID:SGTC_879|Compound:0831-1030|FD-Score:7.11|P-value:3.40E-10 ID:SGTC_933|Compound:2914-0560|FD-Score:4.19|P-value:1.44E-4 ID:SGTC_964|Compound:1287-0107|FD-Score:6.62|P-value:4.61E-9 ID:SGTC_971|Compound:1315-0384|FD-Score:3.99|P-value:2.69E-4 ID:SGTC_1073|Compound:triclabendazole|FD-Score:6.41|P-value:1.31E-8 ID:SGTC_1137|Compound:ixabepilone|FD-Score:3.78|P-value:5.29E-4 ID:SGTC_1154|Compound:4088-0362|FD-Score:3.73|P-value:6.22E-4 ID:SGTC_1213|Compound:1315-0166|FD-Score:-4.03|P-value:2.11E-4 ID:SGTC_1256|Compound:0570-0116|FD-Score:4.46|P-value:5.44E-5 ID:SGTC_1286|Compound:0929-0070|FD-Score:-4.67|P-value:2.31E-5 ID:SGTC_1361|Compound:1608-0041|FD-Score:-3.54|P-value:9.91E-4 ID:SGTC_1364|Compound:1653-0028|FD-Score:-5.02|P-value:5.93E-6 ID:SGTC_1391|Compound:3442-0225|FD-Score:3.86|P-value:4.13E-4 ID:SGTC_1404|Compound:3909-7820|FD-Score:-4.48|P-value:4.55E-5 ID:SGTC_1416|Compound:3966-0321|FD-Score:3.61|P-value:8.92E-4 ID:SGTC_1487|Compound:3970-0795|FD-Score:3.68|P-value:7.15E-4 ID:SGTC_1564|Compound:D-Arginine|FD-Score:3.75|P-value:5.90E-4 ID:SGTC_1622|Compound:st003707|FD-Score:4.63|P-value:3.01E-5 ID:SGTC_1826|Compound:st055655|FD-Score:6.46|P-value:1.01E-8 ID:SGTC_1829|Compound:berberine|FD-Score:-5.03|P-value:5.53E-6 ID:SGTC_1847|Compound:st056231|FD-Score:4.32|P-value:9.00E-5 ID:SGTC_1861|Compound:st057647|FD-Score:4.49|P-value:4.99E-5 ID:SGTC_1885|Compound:st057546|FD-Score:3.99|P-value:2.70E-4 ID:SGTC_1927|Compound:st060202|FD-Score:3.60|P-value:9.04E-4 ID:SGTC_1933|Compound:st074711|FD-Score:3.79|P-value:5.20E-4 ID:SGTC_1982|Compound:st072467|FD-Score:3.91|P-value:3.55E-4 ID:SGTC_2015|Compound:5119600|FD-Score:7.19|P-value:2.11E-10 ID:SGTC_2053|Compound:5265679|FD-Score:4.03|P-value:2.42E-4 ID:SGTC_2062|Compound:5245942|FD-Score:4.27|P-value:1.09E-4 ID:SGTC_2092|Compound:5303694|FD-Score:3.66|P-value:7.55E-4 ID:SGTC_2114|Compound:5491910|FD-Score:3.91|P-value:3.48E-4 ID:SGTC_2178|Compound:5812956|FD-Score:4.44|P-value:5.85E-5 ID:SGTC_2232|Compound:chlorophetanol|FD-Score:7.88|P-value:3.91E-12 ID:SGTC_2254|Compound:7938975|FD-Score:7.09|P-value:3.83E-10 ID:SGTC_2310|Compound:7668677|FD-Score:4.30|P-value:9.73E-5 ID:SGTC_2322|Compound:7413319|FD-Score:5.01|P-value:7.02E-6 ID:SGTC_2347|Compound:9049783|FD-Score:4.11|P-value:1.86E-4 ID:SGTC_2403|Compound:6032726|FD-Score:-4.92|P-value:8.77E-6 ID:SGTC_2407|Compound:paclitaxel|FD-Score:4.17|P-value:1.53E-4 ID:SGTC_2412|Compound:st077135|FD-Score:4.56|P-value:3.82E-5 ID:SGTC_2414|Compound:st077232|FD-Score:-3.91|P-value:3.12E-4 ID:SGTC_2469|Compound:5655019|FD-Score:5.01|P-value:6.97E-6 ID:SGTC_2501|Compound:sodium tetradecyl sulfate|FD-Score:-3.55|P-value:9.63E-4 ID:SGTC_2523|Compound:spermine|FD-Score:3.96|P-value:2.99E-4 ID:SGTC_2792|Compound:7535235|FD-Score:3.61|P-value:8.97E-4 ID:SGTC_2813|Compound:7971799|FD-Score:5.03|P-value:6.39E-6 ID:SGTC_2820|Compound:7989630|FD-Score:3.64|P-value:8.09E-4 ID:SGTC_2828|Compound:7998861|FD-Score:5.19|P-value:3.40E-6 ID:SGTC_2928|Compound:9011484|FD-Score:-3.71|P-value:5.84E-4 ID:SGTC_2951|Compound:9072950|FD-Score:3.85|P-value:4.28E-4 ID:SGTC_3002|Compound:9075603|FD-Score:4.91|P-value:1.04E-5 ID:SGTC_3193|Compound:9111931|FD-Score:5.48|P-value:1.01E-6 ID:SGTC_3212|Compound:9128307|FD-Score:7.06|P-value:4.37E-10 ID:SGTC_3327|Compound:9140648|FD-Score:5.03|P-value:6.40E-6 ID:SGTC_116|Compound:0155-0182|FD-Score:-3.89|P-value:3.42E-4 ID:SGTC_118|Compound:0083-0097|FD-Score:5.24|P-value:2.72E-6 ID:SGTC_146|Compound:3448-8650|FD-Score:6.76|P-value:2.27E-9 ID:SGTC_151|Compound:3013-0144|FD-Score:-3.87|P-value:3.62E-4 ID:SGTC_253|Compound:dyclonine|FD-Score:4.58|P-value:3.53E-5 ID:SGTC_276|Compound:colchicine|FD-Score:4.07|P-value:2.13E-4 ID:SGTC_299|Compound:1273-0059|FD-Score:-3.70|P-value:6.08E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:-4.24|P-value:1.06E-4 ID:SGTC_346|Compound:4513-0042|FD-Score:-5.04|P-value:5.30E-6 ID:SGTC_351|Compound:caspofungin|FD-Score:3.59|P-value:9.37E-4 ID:SGTC_356|Compound:1634-0012|FD-Score:4.34|P-value:8.39E-5 ID:SGTC_360|Compound:0957-0399|FD-Score:4.52|P-value:4.43E-5 ID:SGTC_375|Compound:3970-0961|FD-Score:3.69|P-value:7.00E-4 ID:SGTC_464|Compound:bw-b 70c|FD-Score:3.82|P-value:4.64E-4 ID:SGTC_469|Compound:w7|FD-Score:6.82|P-value:1.58E-9 ID:SGTC_474|Compound:dl-dihydrosphingosine|FD-Score:5.08|P-value:5.31E-6 ID:SGTC_513|Compound:4245-1575|FD-Score:3.60|P-value:9.03E-4 ID:SGTC_679|Compound:0422-0091|FD-Score:3.73|P-value:6.11E-4 ID:SGTC_810|Compound:3825-7150|FD-Score:-4.11|P-value:1.63E-4 ID:SGTC_832|Compound:4083-0019|FD-Score:4.31|P-value:9.52E-5 ID:SGTC_878|Compound:0828-0289|FD-Score:4.83|P-value:1.42E-5 ID:SGTC_879|Compound:0831-1030|FD-Score:7.11|P-value:3.40E-10 ID:SGTC_933|Compound:2914-0560|FD-Score:4.19|P-value:1.44E-4 ID:SGTC_964|Compound:1287-0107|FD-Score:6.62|P-value:4.61E-9 ID:SGTC_971|Compound:1315-0384|FD-Score:3.99|P-value:2.69E-4 ID:SGTC_1073|Compound:triclabendazole|FD-Score:6.41|P-value:1.31E-8 ID:SGTC_1137|Compound:ixabepilone|FD-Score:3.78|P-value:5.29E-4 ID:SGTC_1154|Compound:4088-0362|FD-Score:3.73|P-value:6.22E-4 ID:SGTC_1213|Compound:1315-0166|FD-Score:-4.03|P-value:2.11E-4 ID:SGTC_1256|Compound:0570-0116|FD-Score:4.46|P-value:5.44E-5 ID:SGTC_1286|Compound:0929-0070|FD-Score:-4.67|P-value:2.31E-5 ID:SGTC_1361|Compound:1608-0041|FD-Score:-3.54|P-value:9.91E-4 ID:SGTC_1364|Compound:1653-0028|FD-Score:-5.02|P-value:5.93E-6 ID:SGTC_1391|Compound:3442-0225|FD-Score:3.86|P-value:4.13E-4 ID:SGTC_1404|Compound:3909-7820|FD-Score:-4.48|P-value:4.55E-5 ID:SGTC_1416|Compound:3966-0321|FD-Score:3.61|P-value:8.92E-4 ID:SGTC_1487|Compound:3970-0795|FD-Score:3.68|P-value:7.15E-4 ID:SGTC_1564|Compound:D-Arginine|FD-Score:3.75|P-value:5.90E-4 ID:SGTC_1622|Compound:st003707|FD-Score:4.63|P-value:3.01E-5 ID:SGTC_1826|Compound:st055655|FD-Score:6.46|P-value:1.01E-8 ID:SGTC_1829|Compound:berberine|FD-Score:-5.03|P-value:5.53E-6 ID:SGTC_1847|Compound:st056231|FD-Score:4.32|P-value:9.00E-5 ID:SGTC_1861|Compound:st057647|FD-Score:4.49|P-value:4.99E-5 ID:SGTC_1885|Compound:st057546|FD-Score:3.99|P-value:2.70E-4 ID:SGTC_1927|Compound:st060202|FD-Score:3.60|P-value:9.04E-4 ID:SGTC_1933|Compound:st074711|FD-Score:3.79|P-value:5.20E-4 ID:SGTC_1982|Compound:st072467|FD-Score:3.91|P-value:3.55E-4 ID:SGTC_2015|Compound:5119600|FD-Score:7.19|P-value:2.11E-10 ID:SGTC_2053|Compound:5265679|FD-Score:4.03|P-value:2.42E-4 ID:SGTC_2062|Compound:5245942|FD-Score:4.27|P-value:1.09E-4 ID:SGTC_2092|Compound:5303694|FD-Score:3.66|P-value:7.55E-4 ID:SGTC_2114|Compound:5491910|FD-Score:3.91|P-value:3.48E-4 ID:SGTC_2178|Compound:5812956|FD-Score:4.44|P-value:5.85E-5 ID:SGTC_2232|Compound:chlorophetanol|FD-Score:7.88|P-value:3.91E-12 ID:SGTC_2254|Compound:7938975|FD-Score:7.09|P-value:3.83E-10 ID:SGTC_2310|Compound:7668677|FD-Score:4.30|P-value:9.73E-5 ID:SGTC_2322|Compound:7413319|FD-Score:5.01|P-value:7.02E-6 ID:SGTC_2347|Compound:9049783|FD-Score:4.11|P-value:1.86E-4 ID:SGTC_2403|Compound:6032726|FD-Score:-4.92|P-value:8.77E-6 ID:SGTC_2407|Compound:paclitaxel|FD-Score:4.17|P-value:1.53E-4 ID:SGTC_2412|Compound:st077135|FD-Score:4.56|P-value:3.82E-5 ID:SGTC_2414|Compound:st077232|FD-Score:-3.91|P-value:3.12E-4 ID:SGTC_2469|Compound:5655019|FD-Score:5.01|P-value:6.97E-6 ID:SGTC_2501|Compound:sodium tetradecyl sulfate|FD-Score:-3.55|P-value:9.63E-4 ID:SGTC_2523|Compound:spermine|FD-Score:3.96|P-value:2.99E-4 ID:SGTC_2792|Compound:7535235|FD-Score:3.61|P-value:8.97E-4 ID:SGTC_2813|Compound:7971799|FD-Score:5.03|P-value:6.39E-6 ID:SGTC_2820|Compound:7989630|FD-Score:3.64|P-value:8.09E-4 ID:SGTC_2828|Compound:7998861|FD-Score:5.19|P-value:3.40E-6 ID:SGTC_2928|Compound:9011484|FD-Score:-3.71|P-value:5.84E-4 ID:SGTC_2951|Compound:9072950|FD-Score:3.85|P-value:4.28E-4 ID:SGTC_3002|Compound:9075603|FD-Score:4.91|P-value:1.04E-5 ID:SGTC_3193|Compound:9111931|FD-Score:5.48|P-value:1.01E-6 ID:SGTC_3212|Compound:9128307|FD-Score:7.06|P-value:4.37E-10 ID:SGTC_3327|Compound:9140648|FD-Score:5.03|P-value:6.40E-6

Top fitness defect scores for YFR034C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2232 chlorophetanol 7.88 41.5 µM 3.91E-12 TRP & mitochondrial translation 20 1.64E-15
2 SGTC_2015 5119600 7.19 184.0 µM 2.11E-10 5 3.13E-13
3 SGTC_879 0831-1030 7.11 162.0 µM 3.40E-10 10 5.86E-13
4 SGTC_2254 7938975 7.09 95.2 µM 3.83E-10 15 6.85E-13
5 SGTC_3212 9128307 7.06 49.5 µM 4.37E-10 9 8.14E-13
6 SGTC_469 w7 6.82 172.0 µM 1.58E-9 ERG2 3 4.41E-12
7 SGTC_146 3448-8650 6.76 53.1 µM 2.27E-9 NEO1-PIK1 42 7.13E-12
8 SGTC_964 1287-0107 6.62 19.4 µM 4.61E-9 cell wall 9 1.81E-11
9 SGTC_1826 st055655 6.46 41.6 µM 1.01E-8 ERG2 13 5.10E-11
10 SGTC_1073 triclabendazole 6.41 3.1 µM 1.31E-8 5 7.11E-11
11 SGTC_3193 9111931 5.48 49.5 µM 1.01E-6 6 2.16E-8
12 SGTC_118 0083-0097 5.24 135.3 µM 2.72E-6 translation 45 7.89E-8
13 SGTC_2828 7998861 5.19 71.4 µM 3.40E-6 7 1.06E-7
14 SGTC_474 dl-dihydrosphingosine 5.08 5.0 µM 5.31E-6 15 1.90E-7
15 SGTC_2813 7971799 5.03 71.4 µM 6.39E-6 8 2.42E-7
16 SGTC_3327 9140648 5.03 26.9 µM 6.40E-6 5 2.43E-7
17 SGTC_2469 5655019 5.01 90.8 µM 6.97E-6 10 2.72E-7
18 SGTC_2322 7413319 5.01 38.2 µM 7.02E-6 ERAD & cell cycle 96 2.74E-7
19 SGTC_3002 9075603 4.91 71.4 µM 1.04E-5 6 4.58E-7
20 SGTC_878 0828-0289 4.83 13.7 µM 1.42E-5 26 6.92E-7

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.212 2.91E-35 YML123C PHO84 hom High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p; cells overexpressing Pho84p accumulate heavy metals but do not develop symptoms of metal toxicity
0.141 3.18E-16 YDR162C NBP2 hom Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM)
0.110 2.01E-10 YMR089C YTA12 hom Component, with Afg3p, of the mitochondrial inner membrane m-AAA protease that mediates degradation of misfolded or unassembled proteins and is also required for correct assembly of mitochondrial enzyme complexes
0.095 3.09E-8 YFR051C RET2 het Delta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER
0.091 1.43E-7 YMR202W ERG2 hom C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis
0.090 1.70E-7 YGL246C RAI1 hom Nuclear protein with decapping endonuclease activity targeted toward mRNAs with unmethylated 7-methylguanosine cap structures; binds to and stabilizes the exoribonuclease Rat1p; required for pre-rRNA processing; homologous to human DOM3Z
0.090 1.64E-7 YGR163W GTR2 hom Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD
0.089 2.67E-7 YDR064W RPS13 het Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15
0.086 5.83E-7 YNL002C RLP7 het Nucleolar protein with similarity to large ribosomal subunit L7 proteins; constituent of 66S pre-ribosomal particles; plays an essential role in processing of precursors to the large ribosomal subunit RNAs
0.086 6.20E-7 YBR189W RPS9B hom Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S9 and bacterial S4; RPS9B has a paralog, RPS9A, that arose from the whole genome duplication
0.085 9.16E-7 YBR234C ARC40 het Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches
0.084 1.17E-6 YDR127W ARO1 hom Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids
0.084 1.10E-6 YNL232W CSL4 het Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain an S1 RNA binding domain; has similarity to human hCsl4p (EXOSC1)
0.082 1.75E-6 YLR195C NMT1 het N-myristoyl transferase, catalyzes the cotranslational, covalent attachment of myristic acid to the N-terminal glycine residue of several proteins involved in cellular growth and signal transduction
0.082 2.11E-6 YPL143W RPL33A het Ribosomal 60S subunit protein L33A; N-terminally acetylated; rpl33a null mutant exhibits slow growth while rpl33a rpl33b double null mutant is inviable; homologous to mammalian ribosomal protein L35A, no bacterial homolog; RPL33A has a paralog, RPL33B, that arose from the whole genome duplication