HIPHOP chemogenomics database

YFR034C / PHO4

Basic helix-loop-helix (bHLH) transcription factor of the myc-family; activates transcription cooperatively with Pho2p in response to phosphate limitation; binding to 'CACGTG' motif is regulated by chromatin restriction, competitive binding of Cbf1p to the same DNA binding motif and cooperation with Pho2p,; function is regulated by phosphorylation at multiple sites and by phosphate availability

Zygosity: Homozygous strain

fixedexpanded
Profile for YFR034C / PHO4

Click on Significant Values for Screen Details

Top fitness defect scores for YFR034C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_2232	chlorophetanol	7.88	41.5 µM	3.91E-12	TRP & mitochondrial translation	20	1.64E-15
2	SGTC_2015	5119600	7.19	184.0 µM	2.11E-10		5	3.13E-13
3	SGTC_879	0831-1030	7.11	162.0 µM	3.40E-10		10	5.86E-13
4	SGTC_2254	7938975	7.09	95.2 µM	3.83E-10		15	6.85E-13
5	SGTC_3212	9128307	7.06	49.5 µM	4.37E-10		9	8.14E-13
6	SGTC_469	w7	6.82	172.0 µM	1.58E-9	ERG2	3	4.41E-12
7	SGTC_146	3448-8650	6.76	53.1 µM	2.27E-9	NEO1-PIK1	42	7.13E-12
8	SGTC_964	1287-0107	6.62	19.4 µM	4.61E-9	cell wall	9	1.81E-11
9	SGTC_1826	st055655	6.46	41.6 µM	1.01E-8	ERG2	13	5.10E-11
10	SGTC_1073	triclabendazole	6.41	3.1 µM	1.31E-8		5	7.11E-11
11	SGTC_3193	9111931	5.48	49.5 µM	1.01E-6		6	2.16E-8
12	SGTC_118	0083-0097	5.24	135.3 µM	2.72E-6	translation	45	7.89E-8
13	SGTC_2828	7998861	5.19	71.4 µM	3.40E-6		7	1.06E-7
14	SGTC_474	dl-dihydrosphingosine	5.08	5.0 µM	5.31E-6		15	1.90E-7
15	SGTC_2813	7971799	5.03	71.4 µM	6.39E-6		8	2.42E-7
16	SGTC_3327	9140648	5.03	26.9 µM	6.40E-6		5	2.43E-7
17	SGTC_2469	5655019	5.01	90.8 µM	6.97E-6		10	2.72E-7
18	SGTC_2322	7413319	5.01	38.2 µM	7.02E-6	ERAD & cell cycle	96	2.74E-7
19	SGTC_3002	9075603	4.91	71.4 µM	1.04E-5		6	4.58E-7
20	SGTC_878	0828-0289	4.83	13.7 µM	1.42E-5		26	6.92E-7

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.212	2.91E-35	YML123C	PHO84	hom	High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p; cells overexpressing Pho84p accumulate heavy metals but do not develop symptoms of metal toxicity
0.141	3.18E-16	YDR162C	NBP2	hom	Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM)
0.110	2.01E-10	YMR089C	YTA12	hom	Component, with Afg3p, of the mitochondrial inner membrane m-AAA protease that mediates degradation of misfolded or unassembled proteins and is also required for correct assembly of mitochondrial enzyme complexes
0.095	3.09E-8	YFR051C	RET2	het	Delta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER
0.091	1.43E-7	YMR202W	ERG2	hom	C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis
0.090	1.70E-7	YGL246C	RAI1	hom	Nuclear protein with decapping endonuclease activity targeted toward mRNAs with unmethylated 7-methylguanosine cap structures; binds to and stabilizes the exoribonuclease Rat1p; required for pre-rRNA processing; homologous to human DOM3Z
0.090	1.64E-7	YGR163W	GTR2	hom	Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD
0.089	2.67E-7	YDR064W	RPS13	het	Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15
0.086	5.83E-7	YNL002C	RLP7	het	Nucleolar protein with similarity to large ribosomal subunit L7 proteins; constituent of 66S pre-ribosomal particles; plays an essential role in processing of precursors to the large ribosomal subunit RNAs
0.086	6.20E-7	YBR189W	RPS9B	hom	Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S9 and bacterial S4; RPS9B has a paralog, RPS9A, that arose from the whole genome duplication
0.085	9.16E-7	YBR234C	ARC40	het	Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches
0.084	1.17E-6	YDR127W	ARO1	hom	Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids
0.084	1.10E-6	YNL232W	CSL4	het	Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain an S1 RNA binding domain; has similarity to human hCsl4p (EXOSC1)
0.082	1.75E-6	YLR195C	NMT1	het	N-myristoyl transferase, catalyzes the cotranslational, covalent attachment of myristic acid to the N-terminal glycine residue of several proteins involved in cellular growth and signal transduction
0.082	2.11E-6	YPL143W	RPL33A	het	Ribosomal 60S subunit protein L33A; N-terminally acetylated; rpl33a null mutant exhibits slow growth while rpl33a rpl33b double null mutant is inviable; homologous to mammalian ribosomal protein L35A, no bacterial homolog; RPL33A has a paralog, RPL33B, that arose from the whole genome duplication