YFR050C / PRE4

Beta 7 subunit of the 20S proteasome

Zygosity: Heterozygous strain
fixedexpanded
Profile for YFR050C / PRE4

Click on Significant Values for Screen Details ID:SGTC_36|Compound:0335-0881|FD-Score:8.49|P-value:5.87E-15 ID:SGTC_64|Compound:000l-0018|FD-Score:20.90|P-value:3.37E-81 ID:SGTC_86|Compound:0417-1630|FD-Score:5.99|P-value:2.62E-8 ID:SGTC_137|Compound:0438-0332|FD-Score:14.70|P-value:2.64E-41 ID:SGTC_141|Compound:0443-0274|FD-Score:-4.50|P-value:2.01E-5 ID:SGTC_142|Compound:0335-0849|FD-Score:5.47|P-value:3.41E-7 ID:SGTC_158|Compound:k038-0087|FD-Score:6.76|P-value:4.19E-10 ID:SGTC_165|Compound:k038-0023|FD-Score:5.21|P-value:1.13E-6 ID:SGTC_216|Compound:k038-0035|FD-Score:5.03|P-value:2.49E-6 ID:SGTC_263|Compound:alizarin|FD-Score:5.82|P-value:6.25E-8 ID:SGTC_268|Compound:camptothecin|FD-Score:3.57|P-value:5.99E-4 ID:SGTC_273|Compound:hydroxyurea|FD-Score:4.25|P-value:5.80E-5 ID:SGTC_288|Compound:1315-0019|FD-Score:-3.57|P-value:5.57E-4 ID:SGTC_293|Compound:4049-0273|FD-Score:-4.82|P-value:5.34E-6 ID:SGTC_294|Compound:k038-0071|FD-Score:7.08|P-value:6.27E-11 ID:SGTC_299|Compound:1273-0059|FD-Score:-4.51|P-value:1.94E-5 ID:SGTC_302|Compound:1094-0063|FD-Score:-5.72|P-value:8.96E-8 ID:SGTC_348|Compound:k064-0012|FD-Score:-4.67|P-value:1.01E-5 ID:SGTC_352|Compound:k048-0007|FD-Score:-6.20|P-value:7.67E-9 ID:SGTC_367|Compound:0335-0881|FD-Score:4.23|P-value:6.28E-5 ID:SGTC_373|Compound:0844-0104|FD-Score:-3.85|P-value:2.15E-4 ID:SGTC_383|Compound:2324-0395|FD-Score:-4.52|P-value:1.85E-5 ID:SGTC_444|Compound:curcumin|FD-Score:3.63|P-value:4.98E-4 ID:SGTC_547|Compound:1120-0019|FD-Score:-4.95|P-value:3.15E-6 ID:SGTC_553|Compound:k007-0256|FD-Score:3.48|P-value:8.10E-4 ID:SGTC_609|Compound:0986-0026|FD-Score:3.75|P-value:3.35E-4 ID:SGTC_635|Compound:k038-0028|FD-Score:4.15|P-value:8.27E-5 ID:SGTC_663|Compound:1486-0733|FD-Score:3.60|P-value:5.45E-4 ID:SGTC_667|Compound:1498-1044|FD-Score:4.23|P-value:6.17E-5 ID:SGTC_669|Compound:0108-0021|FD-Score:6.85|P-value:2.36E-10 ID:SGTC_674|Compound:0498-0004|FD-Score:-3.61|P-value:4.78E-4 ID:SGTC_778|Compound:3456-2434|FD-Score:-5.46|P-value:3.07E-7 ID:SGTC_816|Compound:0325-0417|FD-Score:3.89|P-value:2.10E-4 ID:SGTC_827|Compound:3253-0474|FD-Score:3.66|P-value:4.44E-4 ID:SGTC_837|Compound:0091-0265|FD-Score:5.26|P-value:8.74E-7 ID:SGTC_878|Compound:0828-0289|FD-Score:5.93|P-value:3.51E-8 ID:SGTC_879|Compound:0831-1030|FD-Score:3.68|P-value:4.16E-4 ID:SGTC_922|Compound:1866-0035|FD-Score:-3.67|P-value:4.00E-4 ID:SGTC_936|Compound:3005-4620|FD-Score:4.24|P-value:5.87E-5 ID:SGTC_966|Compound:1300-0360|FD-Score:-3.46|P-value:7.77E-4 ID:SGTC_1150|Compound:3909-7983|FD-Score:3.62|P-value:5.20E-4 ID:SGTC_1153|Compound:4055-0057|FD-Score:3.58|P-value:5.93E-4 ID:SGTC_1164|Compound:k060-0068|FD-Score:3.79|P-value:2.93E-4 ID:SGTC_1310|Compound:1188-0297|FD-Score:-3.96|P-value:1.47E-4 ID:SGTC_1347|Compound:1493-0251|FD-Score:-3.45|P-value:8.00E-4 ID:SGTC_1469|Compound:k284-2957|FD-Score:-4.10|P-value:9.09E-5 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:7.48|P-value:5.22E-12 ID:SGTC_1775|Compound:st081588|FD-Score:5.49|P-value:3.14E-7 ID:SGTC_1784|Compound:st039334|FD-Score:-4.78|P-value:6.44E-6 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:-6.58|P-value:9.76E-10 ID:SGTC_1787|Compound:fluconazole|FD-Score:-5.07|P-value:1.83E-6 ID:SGTC_1844|Compound:rapamycin|FD-Score:4.99|P-value:3.01E-6 ID:SGTC_1859|Compound:st055998|FD-Score:3.83|P-value:2.57E-4 ID:SGTC_1994|Compound:st072947|FD-Score:4.08|P-value:1.06E-4 ID:SGTC_2004|Compound:4023728|FD-Score:3.41|P-value:9.81E-4 ID:SGTC_2054|Compound:5233173|FD-Score:-4.62|P-value:1.22E-5 ID:SGTC_2056|Compound:5235370|FD-Score:4.57|P-value:1.71E-5 ID:SGTC_2094|Compound:5332512|FD-Score:4.09|P-value:1.02E-4 ID:SGTC_2249|Compound:haloperidol|FD-Score:4.67|P-value:1.12E-5 ID:SGTC_2250|Compound:alverine citrate|FD-Score:16.90|P-value:1.24E-53 ID:SGTC_2275|Compound:7947463|FD-Score:4.03|P-value:1.28E-4 ID:SGTC_2311|Compound:7694451|FD-Score:4.24|P-value:5.91E-5 ID:SGTC_2341|Compound:7987486|FD-Score:3.46|P-value:8.58E-4 ID:SGTC_2344|Compound:7990960|FD-Score:-3.68|P-value:3.79E-4 ID:SGTC_2345|Compound:9052315|FD-Score:5.01|P-value:2.66E-6 ID:SGTC_2351|Compound:9026858|FD-Score:5.31|P-value:7.16E-7 ID:SGTC_2366|Compound:9070441|FD-Score:5.81|P-value:6.62E-8 ID:SGTC_2679|Compound:rapamycin|FD-Score:6.60|P-value:1.00E-9 ID:SGTC_2688|Compound:kanamycin b|FD-Score:4.58|P-value:1.64E-5 ID:SGTC_2750|Compound:bromebric acid|FD-Score:3.65|P-value:4.68E-4 ID:SGTC_2751|Compound:anetholtrithion|FD-Score:4.03|P-value:1.29E-4 ID:SGTC_2781|Compound:5567276|FD-Score:-4.92|P-value:3.58E-6 ID:SGTC_2784|Compound:7567673|FD-Score:6.32|P-value:4.81E-9 ID:SGTC_2785|Compound:7756463|FD-Score:5.47|P-value:3.41E-7 ID:SGTC_2799|Compound:7812892|FD-Score:-3.95|P-value:1.53E-4 ID:SGTC_2820|Compound:7989630|FD-Score:5.47|P-value:3.38E-7 ID:SGTC_2855|Compound:9016525|FD-Score:-3.54|P-value:6.10E-4 ID:SGTC_2858|Compound:9025781|FD-Score:-4.68|P-value:9.64E-6 ID:SGTC_2925|Compound:9010536|FD-Score:-3.70|P-value:3.55E-4 ID:SGTC_2941|Compound:9056145|FD-Score:-3.65|P-value:4.29E-4 ID:SGTC_2959|Compound:9079155|FD-Score:3.67|P-value:4.43E-4 ID:SGTC_2965|Compound:9082772|FD-Score:-7.56|P-value:2.67E-12 ID:SGTC_3062|Compound:9077494|FD-Score:-8.79|P-value:5.52E-16 ID:SGTC_3103|Compound:9120822|FD-Score:3.41|P-value:9.93E-4 ID:SGTC_3139|Compound:9095020|FD-Score:3.91|P-value:1.92E-4 ID:SGTC_3142|Compound:9086498|FD-Score:22.40|P-value:9.95E-93 ID:SGTC_3197|Compound:9113331|FD-Score:5.12|P-value:1.67E-6 ID:SGTC_3330|Compound:9141424|FD-Score:4.13|P-value:9.07E-5 ID:SGTC_3341|Compound:9147222|FD-Score:4.05|P-value:1.20E-4 ID:SGTC_36|Compound:0335-0881|FD-Score:8.49|P-value:5.87E-15 ID:SGTC_64|Compound:000l-0018|FD-Score:20.90|P-value:3.37E-81 ID:SGTC_86|Compound:0417-1630|FD-Score:5.99|P-value:2.62E-8 ID:SGTC_137|Compound:0438-0332|FD-Score:14.70|P-value:2.64E-41 ID:SGTC_141|Compound:0443-0274|FD-Score:-4.50|P-value:2.01E-5 ID:SGTC_142|Compound:0335-0849|FD-Score:5.47|P-value:3.41E-7 ID:SGTC_158|Compound:k038-0087|FD-Score:6.76|P-value:4.19E-10 ID:SGTC_165|Compound:k038-0023|FD-Score:5.21|P-value:1.13E-6 ID:SGTC_216|Compound:k038-0035|FD-Score:5.03|P-value:2.49E-6 ID:SGTC_263|Compound:alizarin|FD-Score:5.82|P-value:6.25E-8 ID:SGTC_268|Compound:camptothecin|FD-Score:3.57|P-value:5.99E-4 ID:SGTC_273|Compound:hydroxyurea|FD-Score:4.25|P-value:5.80E-5 ID:SGTC_288|Compound:1315-0019|FD-Score:-3.57|P-value:5.57E-4 ID:SGTC_293|Compound:4049-0273|FD-Score:-4.82|P-value:5.34E-6 ID:SGTC_294|Compound:k038-0071|FD-Score:7.08|P-value:6.27E-11 ID:SGTC_299|Compound:1273-0059|FD-Score:-4.51|P-value:1.94E-5 ID:SGTC_302|Compound:1094-0063|FD-Score:-5.72|P-value:8.96E-8 ID:SGTC_348|Compound:k064-0012|FD-Score:-4.67|P-value:1.01E-5 ID:SGTC_352|Compound:k048-0007|FD-Score:-6.20|P-value:7.67E-9 ID:SGTC_367|Compound:0335-0881|FD-Score:4.23|P-value:6.28E-5 ID:SGTC_373|Compound:0844-0104|FD-Score:-3.85|P-value:2.15E-4 ID:SGTC_383|Compound:2324-0395|FD-Score:-4.52|P-value:1.85E-5 ID:SGTC_444|Compound:curcumin|FD-Score:3.63|P-value:4.98E-4 ID:SGTC_547|Compound:1120-0019|FD-Score:-4.95|P-value:3.15E-6 ID:SGTC_553|Compound:k007-0256|FD-Score:3.48|P-value:8.10E-4 ID:SGTC_609|Compound:0986-0026|FD-Score:3.75|P-value:3.35E-4 ID:SGTC_635|Compound:k038-0028|FD-Score:4.15|P-value:8.27E-5 ID:SGTC_663|Compound:1486-0733|FD-Score:3.60|P-value:5.45E-4 ID:SGTC_667|Compound:1498-1044|FD-Score:4.23|P-value:6.17E-5 ID:SGTC_669|Compound:0108-0021|FD-Score:6.85|P-value:2.36E-10 ID:SGTC_674|Compound:0498-0004|FD-Score:-3.61|P-value:4.78E-4 ID:SGTC_778|Compound:3456-2434|FD-Score:-5.46|P-value:3.07E-7 ID:SGTC_816|Compound:0325-0417|FD-Score:3.89|P-value:2.10E-4 ID:SGTC_827|Compound:3253-0474|FD-Score:3.66|P-value:4.44E-4 ID:SGTC_837|Compound:0091-0265|FD-Score:5.26|P-value:8.74E-7 ID:SGTC_878|Compound:0828-0289|FD-Score:5.93|P-value:3.51E-8 ID:SGTC_879|Compound:0831-1030|FD-Score:3.68|P-value:4.16E-4 ID:SGTC_922|Compound:1866-0035|FD-Score:-3.67|P-value:4.00E-4 ID:SGTC_936|Compound:3005-4620|FD-Score:4.24|P-value:5.87E-5 ID:SGTC_966|Compound:1300-0360|FD-Score:-3.46|P-value:7.77E-4 ID:SGTC_1150|Compound:3909-7983|FD-Score:3.62|P-value:5.20E-4 ID:SGTC_1153|Compound:4055-0057|FD-Score:3.58|P-value:5.93E-4 ID:SGTC_1164|Compound:k060-0068|FD-Score:3.79|P-value:2.93E-4 ID:SGTC_1310|Compound:1188-0297|FD-Score:-3.96|P-value:1.47E-4 ID:SGTC_1347|Compound:1493-0251|FD-Score:-3.45|P-value:8.00E-4 ID:SGTC_1469|Compound:k284-2957|FD-Score:-4.10|P-value:9.09E-5 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:7.48|P-value:5.22E-12 ID:SGTC_1775|Compound:st081588|FD-Score:5.49|P-value:3.14E-7 ID:SGTC_1784|Compound:st039334|FD-Score:-4.78|P-value:6.44E-6 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:-6.58|P-value:9.76E-10 ID:SGTC_1787|Compound:fluconazole|FD-Score:-5.07|P-value:1.83E-6 ID:SGTC_1844|Compound:rapamycin|FD-Score:4.99|P-value:3.01E-6 ID:SGTC_1859|Compound:st055998|FD-Score:3.83|P-value:2.57E-4 ID:SGTC_1994|Compound:st072947|FD-Score:4.08|P-value:1.06E-4 ID:SGTC_2004|Compound:4023728|FD-Score:3.41|P-value:9.81E-4 ID:SGTC_2054|Compound:5233173|FD-Score:-4.62|P-value:1.22E-5 ID:SGTC_2056|Compound:5235370|FD-Score:4.57|P-value:1.71E-5 ID:SGTC_2094|Compound:5332512|FD-Score:4.09|P-value:1.02E-4 ID:SGTC_2249|Compound:haloperidol|FD-Score:4.67|P-value:1.12E-5 ID:SGTC_2250|Compound:alverine citrate|FD-Score:16.90|P-value:1.24E-53 ID:SGTC_2275|Compound:7947463|FD-Score:4.03|P-value:1.28E-4 ID:SGTC_2311|Compound:7694451|FD-Score:4.24|P-value:5.91E-5 ID:SGTC_2341|Compound:7987486|FD-Score:3.46|P-value:8.58E-4 ID:SGTC_2344|Compound:7990960|FD-Score:-3.68|P-value:3.79E-4 ID:SGTC_2345|Compound:9052315|FD-Score:5.01|P-value:2.66E-6 ID:SGTC_2351|Compound:9026858|FD-Score:5.31|P-value:7.16E-7 ID:SGTC_2366|Compound:9070441|FD-Score:5.81|P-value:6.62E-8 ID:SGTC_2679|Compound:rapamycin|FD-Score:6.60|P-value:1.00E-9 ID:SGTC_2688|Compound:kanamycin b|FD-Score:4.58|P-value:1.64E-5 ID:SGTC_2750|Compound:bromebric acid|FD-Score:3.65|P-value:4.68E-4 ID:SGTC_2751|Compound:anetholtrithion|FD-Score:4.03|P-value:1.29E-4 ID:SGTC_2781|Compound:5567276|FD-Score:-4.92|P-value:3.58E-6 ID:SGTC_2784|Compound:7567673|FD-Score:6.32|P-value:4.81E-9 ID:SGTC_2785|Compound:7756463|FD-Score:5.47|P-value:3.41E-7 ID:SGTC_2799|Compound:7812892|FD-Score:-3.95|P-value:1.53E-4 ID:SGTC_2820|Compound:7989630|FD-Score:5.47|P-value:3.38E-7 ID:SGTC_2855|Compound:9016525|FD-Score:-3.54|P-value:6.10E-4 ID:SGTC_2858|Compound:9025781|FD-Score:-4.68|P-value:9.64E-6 ID:SGTC_2925|Compound:9010536|FD-Score:-3.70|P-value:3.55E-4 ID:SGTC_2941|Compound:9056145|FD-Score:-3.65|P-value:4.29E-4 ID:SGTC_2959|Compound:9079155|FD-Score:3.67|P-value:4.43E-4 ID:SGTC_2965|Compound:9082772|FD-Score:-7.56|P-value:2.67E-12 ID:SGTC_3062|Compound:9077494|FD-Score:-8.79|P-value:5.52E-16 ID:SGTC_3103|Compound:9120822|FD-Score:3.41|P-value:9.93E-4 ID:SGTC_3139|Compound:9095020|FD-Score:3.91|P-value:1.92E-4 ID:SGTC_3142|Compound:9086498|FD-Score:22.40|P-value:9.95E-93 ID:SGTC_3197|Compound:9113331|FD-Score:5.12|P-value:1.67E-6 ID:SGTC_3330|Compound:9141424|FD-Score:4.13|P-value:9.07E-5 ID:SGTC_3341|Compound:9147222|FD-Score:4.05|P-value:1.20E-4

Top fitness defect scores for YFR050C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_3142 9086498 22.40 49.5 µM 9.95E-93 iron homeostasis 1 1.55E-111 4.30
2 SGTC_64 000l-0018 20.90 272.1 µM 3.37E-81 ubiquinone biosynthesis & proteasome 3 1.26E-97 2.27
3 SGTC_2250 alverine citrate 16.90 130.0 µM 1.24E-53 2 2.31E-64 2.10
4 SGTC_137 0438-0332 14.70 85.4 µM 2.64E-41 ubiquinone biosynthesis & proteasome 5 1.71E-49 1.18
5 SGTC_36 0335-0881 8.49 14.2 µM 5.87E-15 ubiquinone biosynthesis & proteasome 6 1.02E-17 0.17
6 SGTC_1597 7-benzyloxygramine 7.48 47.5 µM 5.22E-12 calcium & mitochondrial duress 24 3.64E-14 0.27
7 SGTC_294 k038-0071 7.08 157.0 µM 6.27E-11 ubiquinone biosynthesis & proteasome 5 7.25E-13 0.03
8 SGTC_669 0108-0021 6.85 26.2 µM 2.36E-10 fatty acid desaturase (OLE1) 2 3.58E-12 0.02
9 SGTC_158 k038-0087 6.76 26.4 µM 4.19E-10 ubiquinone biosynthesis & proteasome 10 7.14E-12 0.04
10 SGTC_2679 rapamycin 6.60 1.0 nM 1.00E-9 23 2.04E-11 0.01
11 SGTC_2784 7567673 6.32 72.7 µM 4.81E-9 iron homeostasis 2 1.35E-10 0.04
12 SGTC_86 0417-1630 5.99 471.9 µM 2.62E-8 ubiquinone biosynthesis & proteasome 11 1.04E-9 0.15
13 SGTC_878 0828-0289 5.93 13.7 µM 3.51E-8 12 1.47E-9 0.45
14 SGTC_263 alizarin 5.82 107.9 µM 6.25E-8 iron homeostasis 3 2.95E-9 0.10
15 SGTC_2366 9070441 5.81 60.6 µM 6.62E-8 2 3.16E-9 0.74
16 SGTC_1775 st081588 5.49 35.2 µM 3.14E-7 60S ribosome export 7 2.05E-8 0.52
17 SGTC_2820 7989630 5.47 71.4 µM 3.38E-7 ERG2 1 2.24E-8 1.20
18 SGTC_142 0335-0849 5.47 62.7 µM 3.41E-7 ubiquinone biosynthesis & proteasome 12 2.26E-8 0.37
19 SGTC_2785 7756463 5.47 72.7 µM 3.41E-7 4 2.27E-8 0.21
20 SGTC_2351 9026858 5.31 72.3 µM 7.16E-7 RNA processing & uracil transport 5 5.53E-8 0.65

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.534 1.07E-246 YOL038W PRE6 het Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress
0.516 2.54E-227 YMR314W PRE5 het Alpha 6 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress
0.510 2.37E-221 YBL041W PRE7 het Beta 6 subunit of the 20S proteasome
0.486 1.14E-198 YER094C PUP3 het Beta 3 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit C10
0.468 9.17E-182 YER012W PRE1 het Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle
0.426 7.80E-148 YOR362C PRE10 het Alpha 7 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress
0.423 2.10E-145 YML092C PRE8 het Alpha 2 subunit of the 20S proteasome
0.400 3.22E-129 YJL001W PRE3 het Beta 1 subunit of the 20S proteasome, responsible for cleavage after acidic residues in peptides
0.392 1.56E-123 YGL011C SCL1 het Alpha 1 subunit of the 20S proteasome involved in the degradation of ubiquitinated substrates; 20S proteasome is the core complex of the 26S proteasome; essential for growth; detected in the mitochondria
0.379 1.02E-114 YLR021W IRC25 hom Component of a heterodimeric Poc4p-Irc25p chaperone involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions
0.358 4.32E-102 YPR103W PRE2 het Beta 5 subunit of the 20S proteasome, responsible for the chymotryptic activity of the proteasome
0.331 1.31E-86 YGR253C PUP2 het Alpha 5 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit zeta
0.301 4.33E-71 YLR201C COQ9 hom Protein required for ubiquinone (coenzyme Q) biosynthesis and respiratory growth; localizes to the matrix face of the mitochondrial inner membrane in a large complex with ubiquinone biosynthetic enzymes
0.299 2.82E-70 YGL119W COQ8 hom Protein required for ubiquinone biosynthesis and respiratory growth; exhibits genetic interaction with COQ9, suggesting a common function; similar to prokaryotic proteins involved in early steps of ubiquinone biosynthesis; COQ8 has a paralog, YBR230W-A, that arose from the whole genome duplication
0.297 3.41E-69 YJL168C SET2 hom Histone methyltransferase with a role in transcriptional elongation; methylates H3 lysine 36, which suppresses incorporation of acetylated histones and signals for the deacetylation of these histones within transcribed genes; associates with the C-terminal domain of Rpo21p; histone methylation activity is regulated by phosphorylation status of Rpo21p