HIPHOP chemogenomics database

YFR051C / RET2

Delta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER

Zygosity: Heterozygous strain

fixedexpanded
Profile for YFR051C / RET2

Click on Significant Values for Screen Details

Top fitness defect scores for YFR051C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value	Screen Clearance
1	SGTC_2232	chlorophetanol	36.60	41.5 µM	5.53E-298	TRP & mitochondrial translation	1	2.11E-293	27.70
2	SGTC_1597	7-benzyloxygramine	21.60	47.5 µM	3.46E-105	calcium & mitochondrial duress	1	1.37E-103	2.63
3	SGTC_1789	tunicamycin	13.40	25.0 nM	9.59E-42	unfolded protein response	4	3.98E-41	4.44
4	SGTC_1306	1185-0270	12.20	9.0 µM	7.29E-35	Golgi	1	2.37E-34	3.92
5	SGTC_3142	9086498	11.60	49.5 µM	6.08E-32	iron homeostasis	6	1.78E-31	0.86
6	SGTC_22	0964-0034	11.20	14.5 µM	9.42E-30	unfolded protein response	2	2.55E-29	2.81
7	SGTC_255	tunicamycin	8.66	200.5 nM	1.35E-18	unfolded protein response	5	2.46E-18	0.31
8	SGTC_245	methotrexate	8.62	289.6 µM	1.87E-18		2	3.39E-18	1.43
9	SGTC_542	3381-0244	8.27	89.3 µM	3.93E-17		2	6.81E-17	1.16
10	SGTC_999	4022-0066	7.91	22.7 µM	7.76E-16	Golgi	2	1.29E-15	0.23
11	SGTC_2038	4100038	7.31	31.0 µM	8.92E-14	plasma membrane duress	2	1.37E-13	1.14
12	SGTC_2021	4019055	7.22	173.0 µM	1.76E-13	endomembrane recycling	13	2.68E-13	0.35
13	SGTC_763	3453-1109	6.48	128.0 µM	3.29E-11		1	4.63E-11	2.06
14	SGTC_103	0844-0104	6.45	51.8 µM	3.86E-11	ERAD & cell cycle	20	5.42E-11	0.04
15	SGTC_524	r073-0010	6.16	27.8 µM	2.72E-10		5	3.71E-10	0.66
16	SGTC_535	2293-4129	5.94	48.2 µM	1.04E-9	NEO1	3	1.38E-9	0.64
17	SGTC_3	0833-0587	5.64	95.6 µM	6.55E-9	redox potentiating	6	8.50E-9	0.76
18	SGTC_848	0368-0089	5.55	85.6 µM	1.08E-8	iron homeostasis	5	1.39E-8	1.00
19	SGTC_1593	justicidin b . 9-(1,3-benzodioxol-5-yl)- 6,7-dimethoxynaphtho[2,3-c]furan-1(3h)-one	5.01	54.9 µM	2.22E-7	redox potentiating	4	2.73E-7	0.90
20	SGTC_733	4069-0007	4.89	56.1 µM	4.12E-7	ERAD & cell cycle	13	5.02E-7	0.02

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.383	2.34E-117	YNL287W	SEC21	het	Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo
0.326	5.99E-84	YDR238C	SEC26	het	Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP)
0.261	2.54E-53	YDL145C	COP1	het	Alpha subunit of COPI vesicle coatomer complex, which surrounds transport vesicles in the early secretory pathway
0.191	7.37E-29	YOR362C	PRE10	het	Alpha 7 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress
0.183	9.01E-27	YBR243C	ALG7	het	UDP-N-acetyl-glucosamine-1-P transferase, transfers Glc-Nac-P from UDP-GlcNac to Dol-P in the ER in the first step of the dolichol pathway of protein asparagine-linked glycosylation; inhibited by tunicamycin
0.177	6.15E-25	YGR098C	ESP1	het	Separase, a caspase-like cysteine protease; promotes sister chromatid separation by mediating dissociation of the cohesin Scc1p from chromatin; inhibits protein phosphatase 2A-Cdc55p to promote mitotic exit; inhibited by Pds1p; relative distribution to the nucleus increases upon DNA replication stress
0.172	1.22E-23	YER122C	GLO3	hom	ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Gcs1p
0.163	1.77E-21	YPL010W	RET3	het	Zeta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER
0.150	2.62E-18	YKR026C	GCN3	hom	Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression
0.150	3.19E-18	YGR253C	PUP2	het	Alpha 5 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit zeta
0.147	1.37E-17	YFL031W	HAC1	hom	Basic leucine zipper (bZIP) transcription factor (ATF/CREB1 homolog); regulates the unfolded protein response, via UPRE binding, and membrane biogenesis; ER stress-induced splicing pathway facilitates efficient Hac1p synthesis; protein abundance increases in response to DNA replication stress
0.143	9.72E-17	YER151C	UBP3	hom	Ubiquitin-specific protease involved in transport and osmotic response; interacts with Bre5p to co-regulate anterograde and retrograde transport between the ER and Golgi; involved in transcription elongation in response to osmostress through phosphorylation at Ser695 by Hog1p; inhibitor of gene silencing; cleaves ubiquitin fusions but not polyubiquitin; also has mRNA binding activity; protein abundance increases in response to DNA replication stress
0.138	1.12E-15	YDR008C_d	YDR008C_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.134	7.63E-15	YKL058W	TOA2	het	TFIIA small subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to smallest subunit of human and Drosophila TFIIA; protein abundance increases in response to DNA replication stress
0.133	9.18E-15	YGL026C	TRP5	hom	Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis