HIPHOP chemogenomics database

YFR053C / HXK1

Hexokinase isoenzyme 1; a cytosolic protein that catalyzes phosphorylation of glucose during glucose metabolism; expression is highest during growth on non-glucose carbon sources; glucose-induced repression involves hexokinase Hxk2p; HXK1 has a paralog, HXK2, that arose from the whole genome duplication

Zygosity: Homozygous strain

fixedexpanded
Profile for YFR053C / HXK1

Click on Significant Values for Screen Details

Top fitness defect scores for YFR053C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_392	N-ethylmaleimide	7.37	8.3 µM	8.70E-13	RNA pol III & RNase P/MRP	1	8.71E-14
2	SGTC_1135	dihydrocapsaicin	6.58	536.7 nM	1.52E-10		4	2.34E-11
3	SGTC_393	acivicin	6.53	6.2 µM	2.03E-10		5	3.20E-11
4	SGTC_1749	st037932	5.46	54.2 µM	9.11E-8		12	2.40E-8
5	SGTC_1951	sophoraflavanone G	5.04	9.2 µM	7.28E-7	RNA processing & uracil transport	5	2.28E-7
6	SGTC_3307	9126885	4.95	69.3 µM	1.12E-6		17	3.63E-7
7	SGTC_1005	4092-0690	4.91	4.4 µM	1.40E-6	plasma membrane duress	16	4.64E-7
8	SGTC_2117	5521686	4.81	200.0 µM	2.19E-6		6	7.52E-7
9	SGTC_464	bw-b 70c	4.62	158.0 µM	5.24E-6		10	1.94E-6
10	SGTC_907	3448-6986	4.45	75.5 µM	1.10E-5		16	4.33E-6
11	SGTC_657	3534-0529	4.21	86.7 µM	2.93E-5		10	1.25E-5
12	SGTC_1928	4'-methoxychalcone	4.19	40.3 µM	3.25E-5		7	1.40E-5
13	SGTC_1024	4507-0395	4.04	26.0 µM	5.78E-5	TSC3-RPN4	24	2.62E-5
14	SGTC_382	3229-0966	4.02	23.2 µM	6.33E-5		18	2.89E-5
15	SGTC_1366	1683-0113	3.97	21.9 µM	7.78E-5		12	3.61E-5
16	SGTC_1791	st048313	3.95	56.1 µM	8.25E-5		15	3.85E-5
17	SGTC_1685	st024049	3.92	53.1 µM	9.36E-5	TSC3-RPN4	15	4.42E-5
18	SGTC_1775	st081588	3.84	35.2 µM	1.29E-4	60S ribosome export	25	6.27E-5
19	SGTC_3316	9136639	3.78	10.6 µM	1.57E-4		16	7.76E-5
20	SGTC_881	0833-0569	3.78	170.0 µM	1.61E-4		39	7.96E-5

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.148	7.47E-18	YPR035W	GLN1	het	Glutamine synthetase (GS); synthesizes glutamine from glutamate and ammonia; with Glt1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source and by amino acid limitation; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
0.108	3.88E-10	YMR254C_d	YMR254C_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.100	6.86E-9	YJR103W	URA8	hom	Minor CTP synthase isozyme (see also URA7); catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to UTP, forming CTP, the final step in de novo biosynthesis of pyrimidines; involved in phospholipid biosynthesis; capable of forming cytoplasmic filaments termed cytoophidium, especially during conditions of glucose depletion; URA8 has a paralog, URA7, that arose from the whole genome duplication
0.087	4.62E-7	YLR291C	GCD7	het	Beta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
0.082	2.16E-6	YFR046C	CNN1	hom	Kinetochore protein; associated with the essential kinetochore proteins Nnf1p and Spc24p; phosphorylated by Clb5-Cdk1, Mps1p, Ipl1p and to a lesser extent by Clb2-Cdk1; localizes to the lower region of the Ndc80 complex during anaphase and regulates KMN activity by inhibiting the Mtw1 and Spc105 complexes from binding to the Ndc80 complex; similar to metazoan CENP-T
0.081	2.52E-6	YOR287C	RRP36	het	Component of 90S preribosomes; involved in early cleavages of the 35S pre-rRNA and in production of the 40S ribosomal subunit
0.076	1.06E-5	YMR049C	ERB1	het	Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Ytm1p that is required for maturation of the large ribosomal subunit; required for maturation of the 25S and 5.8S ribosomal RNAs; homologous to mammalian Bop1
0.073	2.48E-5	YEL072W	RMD6	hom	Protein required for sporulation
0.069	6.94E-5	YMR261C	TPS3	hom	Regulatory subunit of trehalose-6-phosphate synthase/phosphatase; involved in synthesis of storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway; TPS3 has a paralog, TSL1, that arose from the whole genome duplication
0.066	1.34E-4	YPL081W	RPS9A	hom	Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S9 and bacterial S4; RPS9A has a paralog, RPS9B, that arose from the whole genome duplication
0.066	1.43E-4	YNR056C	BIO5	hom	Putative transmembrane protein involved in the biotin biosynthesis pathway; responsible for uptake of 7-keto 8-aminopelargonic acid; BIO5 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis
0.066	1.44E-4	YEL041W	YEF1	hom	ATP-NADH kinase; phosphorylates both NAD and NADH; homooctameric structure consisting of 60-kDa subunits; similar to Pos5p; overexpression complements certain pos5 phenotypes; YEF1 has a paralog, UTR1, that arose from the whole genome duplication
0.065	1.58E-4	YHR015W	MIP6	hom	Putative RNA-binding protein; interacts with Mex67p, which is a component of the nuclear pore involved in nuclear mRNA export; MIP6 has a paralog, PES4, that arose from the whole genome duplication
0.065	1.75E-4	YLR303W	MET17	hom	Methionine and cysteine synthase (O-acetyl homoserine-O-acetyl serine sulfhydrylase), required for sulfur amino acid synthesis
0.064	2.06E-4	YBL028C	YBL028C	hom	Protein of unknown function that may interact with ribosomes; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; predicted to be involved in ribosome biogenesis