YGL004C / RPN14

Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasome regulatory particle (RP); null mutants accumulate ubiquitinated Gcn4p and display decreased 26S proteasome stability; interacts with Rpt5p

Zygosity: Homozygous strain
fixedexpanded
Profile for YGL004C / RPN14

Click on Significant Values for Screen Details ID:SGTC_6|Compound:0844-0013|FD-Score:3.99|P-value:5.08E-5 ID:SGTC_38|Compound:0929-0051|FD-Score:-3.53|P-value:2.14E-4 ID:SGTC_66|Compound:0109-0187|FD-Score:-8.38|P-value:5.37E-17 ID:SGTC_447|Compound:ml7|FD-Score:3.86|P-value:8.55E-5 ID:SGTC_448|Compound:monastrol|FD-Score:6.32|P-value:3.20E-10 ID:SGTC_822|Compound:1487-0950|FD-Score:-3.42|P-value:3.19E-4 ID:SGTC_835|Compound:0312-0010|FD-Score:3.65|P-value:1.89E-4 ID:SGTC_843|Compound:0310-0051|FD-Score:4.02|P-value:4.55E-5 ID:SGTC_850|Compound:0388-0143|FD-Score:3.36|P-value:5.56E-4 ID:SGTC_962|Compound:1254-0300|FD-Score:3.36|P-value:5.56E-4 ID:SGTC_1169|Compound:aniline yellow|FD-Score:-3.43|P-value:3.12E-4 ID:SGTC_1705|Compound:st027868|FD-Score:-3.77|P-value:8.58E-5 ID:SGTC_1935|Compound:st074532|FD-Score:-3.24|P-value:6.11E-4 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:3.58|P-value:2.45E-4 ID:SGTC_2762|Compound:leflunomide|FD-Score:3.87|P-value:8.43E-5 ID:SGTC_3156|Compound:9099931|FD-Score:3.99|P-value:5.03E-5 ID:SGTC_6|Compound:0844-0013|FD-Score:3.99|P-value:5.08E-5 ID:SGTC_38|Compound:0929-0051|FD-Score:-3.53|P-value:2.14E-4 ID:SGTC_66|Compound:0109-0187|FD-Score:-8.38|P-value:5.37E-17 ID:SGTC_447|Compound:ml7|FD-Score:3.86|P-value:8.55E-5 ID:SGTC_448|Compound:monastrol|FD-Score:6.32|P-value:3.20E-10 ID:SGTC_822|Compound:1487-0950|FD-Score:-3.42|P-value:3.19E-4 ID:SGTC_835|Compound:0312-0010|FD-Score:3.65|P-value:1.89E-4 ID:SGTC_843|Compound:0310-0051|FD-Score:4.02|P-value:4.55E-5 ID:SGTC_850|Compound:0388-0143|FD-Score:3.36|P-value:5.56E-4 ID:SGTC_962|Compound:1254-0300|FD-Score:3.36|P-value:5.56E-4 ID:SGTC_1169|Compound:aniline yellow|FD-Score:-3.43|P-value:3.12E-4 ID:SGTC_1705|Compound:st027868|FD-Score:-3.77|P-value:8.58E-5 ID:SGTC_1935|Compound:st074532|FD-Score:-3.24|P-value:6.11E-4 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:3.58|P-value:2.45E-4 ID:SGTC_2762|Compound:leflunomide|FD-Score:3.87|P-value:8.43E-5 ID:SGTC_3156|Compound:9099931|FD-Score:3.99|P-value:5.03E-5

Top fitness defect scores for YGL004C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_448 monastrol 6.32 171.0 µM 3.20E-10 2 1.30E-10
2 SGTC_843 0310-0051 4.02 143.0 µM 4.55E-5 10 2.93E-5
3 SGTC_3156 9099931 3.99 49.5 µM 5.03E-5 7 3.25E-5
4 SGTC_6 0844-0013 3.99 19.8 µM 5.08E-5 84 3.28E-5
5 SGTC_2762 leflunomide 3.87 42.1 µM 8.43E-5 34 5.55E-5
6 SGTC_447 ml7 3.86 120.0 µM 8.55E-5 NEO1-PIK1 29 5.64E-5
7 SGTC_835 0312-0010 3.65 67.2 µM 1.89E-4 16 1.29E-4
8 SGTC_2494 18-aminoabieta-8,11,13-triene sulfate 3.58 3.7 µM 2.45E-4 117 1.69E-4
9 SGTC_962 1254-0300 3.36 21.5 µM 5.56E-4 43 3.96E-4
10 SGTC_850 0388-0143 3.36 75.9 µM 5.56E-4 28 3.96E-4
11 SGTC_837 0091-0265 3.01 12.5 µM 0.00174 ubiquinone biosynthesis & proteasome 60 0.00130
12 SGTC_1679 4130-1276 2.91 6.7 µM 0.00236 Golgi 128 0.00179
13 SGTC_966 1300-0360 2.91 30.2 µM 0.00242 heme requiring 75 0.00183
14 SGTC_860 0443-0016 2.90 25.1 µM 0.00246 71 0.00187
15 SGTC_3023 9089636 2.86 49.5 µM 0.00274 32 0.00209
16 SGTC_1555 citronellol 2.81 128.0 µM 0.00321 32 0.00246
17 SGTC_2031 5180455 2.81 200.0 µM 0.00324 55 0.00248
18 SGTC_1262 0737-0114 2.75 49.6 µM 0.00380 95 0.00294
19 SGTC_1756 st041558 2.74 35.5 µM 0.00403 47 0.00312
20 SGTC_845 0330-0138 2.70 4.5 µM 0.00444 81 0.00345

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.401 6.90E-130 YLR414C PUN1 hom Plasma membrane protein with a role in cell wall integrity; co-localizes with Sur7p in punctate membrane patches; null mutant displays decreased thermotolerance; transcription induced upon cell wall damage and metal ion stress
0.279 8.93E-61 YKL102C_d YKL102C_d hom Dubious open reading frame unlikely to encode a functional protein; deletion confers sensitivity to citric acid; predicted protein would include a thiol-disulfide oxidoreductase active site
0.256 3.28E-51 YGR139W_d YGR139W_d hom Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.242 8.16E-46 YNL242W ATG2 hom Peripheral membrane protein required for autophagic vesicle formation; also required for vesicle formation during pexophagy and the cytoplasm-to-vaucole targeting (Cvt) pathway; involved in Atg9p cycling between the phagophore assembly site and mitochondria; forms cytoplasmic foci upon DNA replication stress
0.233 1.83E-42 YLR263W RED1 hom Protein component of the synaptonemal complex axial elements, involved in chromosome segregation during the first meiotic division; critical for coupling checkpoint signaling to SC formation; interacts with Hop1p, Mec3p and Ddc1p
0.209 2.48E-34 YDL049C KNH1 hom Protein with similarity to Kre9p, which is involved in cell wall beta 1,6-glucan synthesis; overproduction suppresses growth defects of a kre9 null mutant; required for propionic acid resistance
0.203 2.01E-32 YJL058C BIT61 hom Subunit of TORC2 membrane-associated complex; involved in regulation of cell cycle-dependent actin cytoskeletal dynamics during polarized growth and cell wall integrity; BIT61 has a paralog, BIT2, that arose from the whole genome duplication
0.201 9.33E-32 YOR113W AZF1 hom Zinc-finger transcription factor; in the presence of glucose, activates transcription of genes involved in growth and carbon metabolism; in nonfermentable carbon sources, activates transcription of genes involved in maintenance of cell wall integrity
0.190 1.70E-28 YLR405W DUS4 hom Dihydrouridine synthase, member of a widespread family of conserved proteins including Smm1p, Dus1p, and Dus3p
0.184 8.93E-27 YDR100W TVP15 hom Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p
0.183 1.04E-26 YLR398C SKI2 hom Ski complex component and putative RNA helicase, mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay
0.170 3.20E-23 YPL223C GRE1 hom Hydrophilin essential in dessication-rehydration process; stress induced (osmotic, ionic, oxidative, heat shock and heavy metals); regulated by the HOG pathway
0.169 6.65E-23 YDR384C ATO3 hom Plasma membrane protein, regulation pattern suggests a possible role in export of ammonia from the cell; phosphorylated in mitochondria; member of the TC 9.B.33 YaaH family of putative transporters
0.168 1.17E-22 YNL097C-B_p YNL097C-B_p hom Putative protein of unknown function
0.168 1.30E-22 YMR041C ARA2 hom NAD-dependent arabinose dehydrogenase, involved in biosynthesis of dehydro-D-arabinono-1,4-lactone; similar to plant L-galactose dehydrogenase