YGL007W_d / BRP1

Dubious ORF located in the upstream region of PMA1, deletion leads to polyamine resistance due to downregulation of PMA1

Zygosity: Homozygous strain
fixedexpanded
Profile for YGL007W / BRP1

Click on Significant Values for Screen Details ID:SGTC_28|Compound:pentachloropyridine N-oxide|FD-Score:4.21|P-value:3.95E-4 ID:SGTC_50|Compound:0929-0078|FD-Score:7.35|P-value:2.09E-9 ID:SGTC_58|Compound:0957-0393|FD-Score:6.10|P-value:5.55E-7 ID:SGTC_129|Compound:0088-0017|FD-Score:7.55|P-value:7.94E-10 ID:SGTC_153|Compound:4077-0274|FD-Score:4.52|P-value:1.57E-4 ID:SGTC_167|Compound:3672-0051|FD-Score:-5.35|P-value:7.56E-6 ID:SGTC_204|Compound:1543-0184|FD-Score:11.80|P-value:1.85E-21 ID:SGTC_256|Compound:clotrimazole|FD-Score:-4.70|P-value:7.15E-5 ID:SGTC_296|Compound:1222-0039|FD-Score:5.14|P-value:2.05E-5 ID:SGTC_398|Compound:bronopol|FD-Score:7.92|P-value:1.23E-10 ID:SGTC_432|Compound:NS 1619|FD-Score:4.58|P-value:1.31E-4 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:4.51|P-value:1.60E-4 ID:SGTC_498|Compound:2,5-di-tert-butyl-1,4-hydroquinone (DTBHQ)|FD-Score:7.31|P-value:2.62E-9 ID:SGTC_666|Compound:0272-0154|FD-Score:4.53|P-value:1.52E-4 ID:SGTC_677|Compound:0929-0063|FD-Score:5.04|P-value:2.92E-5 ID:SGTC_693|Compound:4161-2696|FD-Score:3.95|P-value:8.40E-4 ID:SGTC_736|Compound:0958-0254|FD-Score:6.20|P-value:3.72E-7 ID:SGTC_767|Compound:0929-0023|FD-Score:10.40|P-value:3.94E-17 ID:SGTC_819|Compound:1013-0258|FD-Score:7.82|P-value:1.98E-10 ID:SGTC_892|Compound:0929-0076|FD-Score:9.02|P-value:2.68E-13 ID:SGTC_908|Compound:3448-7491|FD-Score:5.92|P-value:1.13E-6 ID:SGTC_928|Compound:2764-0428|FD-Score:5.47|P-value:6.46E-6 ID:SGTC_955|Compound:1216-0110|FD-Score:4.29|P-value:3.19E-4 ID:SGTC_997|Compound:3996-0078|FD-Score:4.09|P-value:5.69E-4 ID:SGTC_1121|Compound:fusaric acid|FD-Score:4.21|P-value:4.03E-4 ID:SGTC_1233|Compound:0325-0408|FD-Score:5.58|P-value:4.16E-6 ID:SGTC_1333|Compound:1482-0047|FD-Score:6.03|P-value:7.45E-7 ID:SGTC_1565|Compound:sinapyl alcohol|FD-Score:4.30|P-value:3.04E-4 ID:SGTC_1697|Compound:st025554|FD-Score:6.05|P-value:6.84E-7 ID:SGTC_1712|Compound:st032288|FD-Score:-4.73|P-value:6.58E-5 ID:SGTC_1881|Compound:5652484|FD-Score:15.10|P-value:3.82E-34 ID:SGTC_1883|Compound:5-benzylidenerhodanine|FD-Score:14.20|P-value:4.02E-30 ID:SGTC_1913|Compound:st060213|FD-Score:-4.79|P-value:5.39E-5 ID:SGTC_1914|Compound:st062367|FD-Score:-4.40|P-value:1.84E-4 ID:SGTC_1921|Compound:5740136|FD-Score:13.70|P-value:2.45E-28 ID:SGTC_1922|Compound:5578187|FD-Score:10.20|P-value:1.33E-16 ID:SGTC_1950|Compound:st077106|FD-Score:5.65|P-value:3.18E-6 ID:SGTC_1951|Compound:sophoraflavanone G|FD-Score:4.82|P-value:6.00E-5 ID:SGTC_1986|Compound:st071812|FD-Score:4.06|P-value:6.06E-4 ID:SGTC_2001|Compound:4023502|FD-Score:4.81|P-value:6.25E-5 ID:SGTC_2077|Compound:5212015|FD-Score:4.32|P-value:2.90E-4 ID:SGTC_2111|Compound:5486399|FD-Score:4.27|P-value:3.30E-4 ID:SGTC_2165|Compound:5727197|FD-Score:11.20|P-value:1.29E-19 ID:SGTC_2416|Compound:tofa|FD-Score:5.79|P-value:1.88E-6 ID:SGTC_2426|Compound:5582046|FD-Score:4.17|P-value:4.44E-4 ID:SGTC_2447|Compound:5741742|FD-Score:4.79|P-value:6.59E-5 ID:SGTC_2472|Compound:5377652|FD-Score:6.74|P-value:3.58E-8 ID:SGTC_2486|Compound:5283202|FD-Score:5.00|P-value:3.34E-5 ID:SGTC_2488|Compound:5268135|FD-Score:7.39|P-value:1.71E-9 ID:SGTC_2582|Compound:agaric acid|FD-Score:9.31|P-value:4.63E-14 ID:SGTC_2615|Compound:purpurogallin-4-carboxylic acid|FD-Score:7.53|P-value:8.66E-10 ID:SGTC_2625|Compound:euphol|FD-Score:-6.80|P-value:2.05E-8 ID:SGTC_2686|Compound:alizarin|FD-Score:5.53|P-value:5.02E-6 ID:SGTC_2752|Compound:diclazuril|FD-Score:4.63|P-value:1.12E-4 ID:SGTC_2762|Compound:leflunomide|FD-Score:4.37|P-value:2.52E-4 ID:SGTC_2858|Compound:9025781|FD-Score:3.96|P-value:7.98E-4 ID:SGTC_3143|Compound:9086658|FD-Score:5.76|P-value:2.17E-6 ID:SGTC_28|Compound:pentachloropyridine N-oxide|FD-Score:4.21|P-value:3.95E-4 ID:SGTC_50|Compound:0929-0078|FD-Score:7.35|P-value:2.09E-9 ID:SGTC_58|Compound:0957-0393|FD-Score:6.10|P-value:5.55E-7 ID:SGTC_129|Compound:0088-0017|FD-Score:7.55|P-value:7.94E-10 ID:SGTC_153|Compound:4077-0274|FD-Score:4.52|P-value:1.57E-4 ID:SGTC_167|Compound:3672-0051|FD-Score:-5.35|P-value:7.56E-6 ID:SGTC_204|Compound:1543-0184|FD-Score:11.80|P-value:1.85E-21 ID:SGTC_256|Compound:clotrimazole|FD-Score:-4.70|P-value:7.15E-5 ID:SGTC_296|Compound:1222-0039|FD-Score:5.14|P-value:2.05E-5 ID:SGTC_398|Compound:bronopol|FD-Score:7.92|P-value:1.23E-10 ID:SGTC_432|Compound:NS 1619|FD-Score:4.58|P-value:1.31E-4 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:4.51|P-value:1.60E-4 ID:SGTC_498|Compound:2,5-di-tert-butyl-1,4-hydroquinone (DTBHQ)|FD-Score:7.31|P-value:2.62E-9 ID:SGTC_666|Compound:0272-0154|FD-Score:4.53|P-value:1.52E-4 ID:SGTC_677|Compound:0929-0063|FD-Score:5.04|P-value:2.92E-5 ID:SGTC_693|Compound:4161-2696|FD-Score:3.95|P-value:8.40E-4 ID:SGTC_736|Compound:0958-0254|FD-Score:6.20|P-value:3.72E-7 ID:SGTC_767|Compound:0929-0023|FD-Score:10.40|P-value:3.94E-17 ID:SGTC_819|Compound:1013-0258|FD-Score:7.82|P-value:1.98E-10 ID:SGTC_892|Compound:0929-0076|FD-Score:9.02|P-value:2.68E-13 ID:SGTC_908|Compound:3448-7491|FD-Score:5.92|P-value:1.13E-6 ID:SGTC_928|Compound:2764-0428|FD-Score:5.47|P-value:6.46E-6 ID:SGTC_955|Compound:1216-0110|FD-Score:4.29|P-value:3.19E-4 ID:SGTC_997|Compound:3996-0078|FD-Score:4.09|P-value:5.69E-4 ID:SGTC_1121|Compound:fusaric acid|FD-Score:4.21|P-value:4.03E-4 ID:SGTC_1233|Compound:0325-0408|FD-Score:5.58|P-value:4.16E-6 ID:SGTC_1333|Compound:1482-0047|FD-Score:6.03|P-value:7.45E-7 ID:SGTC_1565|Compound:sinapyl alcohol|FD-Score:4.30|P-value:3.04E-4 ID:SGTC_1697|Compound:st025554|FD-Score:6.05|P-value:6.84E-7 ID:SGTC_1712|Compound:st032288|FD-Score:-4.73|P-value:6.58E-5 ID:SGTC_1881|Compound:5652484|FD-Score:15.10|P-value:3.82E-34 ID:SGTC_1883|Compound:5-benzylidenerhodanine|FD-Score:14.20|P-value:4.02E-30 ID:SGTC_1913|Compound:st060213|FD-Score:-4.79|P-value:5.39E-5 ID:SGTC_1914|Compound:st062367|FD-Score:-4.40|P-value:1.84E-4 ID:SGTC_1921|Compound:5740136|FD-Score:13.70|P-value:2.45E-28 ID:SGTC_1922|Compound:5578187|FD-Score:10.20|P-value:1.33E-16 ID:SGTC_1950|Compound:st077106|FD-Score:5.65|P-value:3.18E-6 ID:SGTC_1951|Compound:sophoraflavanone G|FD-Score:4.82|P-value:6.00E-5 ID:SGTC_1986|Compound:st071812|FD-Score:4.06|P-value:6.06E-4 ID:SGTC_2001|Compound:4023502|FD-Score:4.81|P-value:6.25E-5 ID:SGTC_2077|Compound:5212015|FD-Score:4.32|P-value:2.90E-4 ID:SGTC_2111|Compound:5486399|FD-Score:4.27|P-value:3.30E-4 ID:SGTC_2165|Compound:5727197|FD-Score:11.20|P-value:1.29E-19 ID:SGTC_2416|Compound:tofa|FD-Score:5.79|P-value:1.88E-6 ID:SGTC_2426|Compound:5582046|FD-Score:4.17|P-value:4.44E-4 ID:SGTC_2447|Compound:5741742|FD-Score:4.79|P-value:6.59E-5 ID:SGTC_2472|Compound:5377652|FD-Score:6.74|P-value:3.58E-8 ID:SGTC_2486|Compound:5283202|FD-Score:5.00|P-value:3.34E-5 ID:SGTC_2488|Compound:5268135|FD-Score:7.39|P-value:1.71E-9 ID:SGTC_2582|Compound:agaric acid|FD-Score:9.31|P-value:4.63E-14 ID:SGTC_2615|Compound:purpurogallin-4-carboxylic acid|FD-Score:7.53|P-value:8.66E-10 ID:SGTC_2625|Compound:euphol|FD-Score:-6.80|P-value:2.05E-8 ID:SGTC_2686|Compound:alizarin|FD-Score:5.53|P-value:5.02E-6 ID:SGTC_2752|Compound:diclazuril|FD-Score:4.63|P-value:1.12E-4 ID:SGTC_2762|Compound:leflunomide|FD-Score:4.37|P-value:2.52E-4 ID:SGTC_2858|Compound:9025781|FD-Score:3.96|P-value:7.98E-4 ID:SGTC_3143|Compound:9086658|FD-Score:5.76|P-value:2.17E-6

Top fitness defect scores for YGL007W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1881 5652484 15.10 16.0 µM 3.82E-34 TRP & mitochondrial translation 3 4.78E-52
2 SGTC_1883 5-benzylidenerhodanine 14.20 20.0 µM 4.02E-30 TRP & mitochondrial translation 7 8.22E-46
3 SGTC_1921 5740136 13.70 50.0 µM 2.45E-28 TRP & mitochondrial translation 1 4.81E-43
4 SGTC_204 1543-0184 11.80 62.4 µM 1.85E-21 TRP & mitochondrial translation 1 2.19E-32
5 SGTC_2165 5727197 11.20 32.1 µM 1.29E-19 2 1.57E-29
6 SGTC_767 0929-0023 10.40 86.0 µM 3.94E-17 TRP & mitochondrial translation 4 1.11E-25
7 SGTC_1922 5578187 10.20 25.0 µM 1.33E-16 TRP & mitochondrial translation 1 7.25E-25
8 SGTC_2582 agaric acid 9.31 100.0 µM 4.63E-14 redox potentiating 79 6.24E-21
9 SGTC_892 0929-0076 9.02 29.0 µM 2.68E-13 TRP & mitochondrial translation 1 9.45E-20
10 SGTC_398 bronopol 7.92 33.3 µM 1.23E-10 RNA processing & uracil transport 4 1.23E-15
11 SGTC_819 1013-0258 7.82 27.7 µM 1.98E-10 14 2.56E-15
12 SGTC_129 0088-0017 7.55 176.6 µM 7.94E-10 20 2.19E-14
13 SGTC_2615 purpurogallin-4-carboxylic acid 7.53 100.0 µM 8.66E-10 redox potentiating 19 2.50E-14
14 SGTC_2488 5268135 7.39 45.7 µM 1.71E-9 15 7.17E-14
15 SGTC_50 0929-0078 7.35 56.4 µM 2.09E-9 18 9.73E-14
16 SGTC_498 2,5-di-tert-butyl-1,4-hydroquinone (DTBHQ) 7.31 150.0 µM 2.62E-9 Golgi 10 1.38E-13
17 SGTC_2472 5377652 6.74 49.4 µM 3.58E-8 TRP & mitochondrial translation 17 7.82E-12
18 SGTC_736 0958-0254 6.20 92.6 µM 3.72E-7 Golgi 4 2.88E-10
19 SGTC_58 0957-0393 6.10 9.0 µM 5.55E-7 cell wall 209 5.33E-10
20 SGTC_1697 st025554 6.05 27.7 µM 6.84E-7 13 7.34E-10

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.450 1.00E-166 YGL008C PMA1 het Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plasma membrane potential; P2-type ATPase; Hsp30p plays a role in Pma1p regulation; interactions with Std1p appear to propagate [GAR+]
0.330 5.82E-86 YKL077W_p YKL077W_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole
0.191 5.72E-29 YGL026C TRP5 hom Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis
0.187 9.41E-28 YDR007W TRP1 hom Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA)
0.170 3.46E-23 YEL009C GCN4 hom Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels
0.162 3.57E-21 YGR037C ACB1 hom Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress
0.154 2.46E-19 YNL184C_p YNL184C_p hom Protein of unknown function; expressed at both mRNA and protein levels
0.151 1.42E-18 YBR002C RER2 het Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting
0.150 2.51E-18 YDR414C ERD1 hom Predicted membrane protein required for the retention of lumenal endoplasmic reticulum proteins; mutants secrete the endogenous ER protein, BiP (Kar2p)
0.150 3.00E-18 YDR203W_d YDR203W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.147 1.32E-17 YOR162C YRR1 hom Zn2-Cys6 zinc-finger transcription factor; activates genes involved in multidrug resistance; paralog of Yrm1p, acting on an overlapping set of target genes; YRR1 has a paralog, PDR8, that arose from the whole genome duplication
0.143 7.27E-17 YFR009W GCN20 hom Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA
0.140 3.94E-16 YDR008C_d YDR008C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.139 5.27E-16 YBR039W ATP3 hom Gamma subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis
0.137 1.64E-15 YGL007C-A_p YGL007C-A_p hom Putative protein of unknown function, identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; deletion exhibits slow-growth phenotype; computationally predicted to have a role in cell budding