YGL008C / PMA1

Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plasma membrane potential; P2-type ATPase; Hsp30p plays a role in Pma1p regulation; interactions with Std1p appear to propagate [GAR+]

Zygosity: Heterozygous strain
fixedexpanded
Profile for YGL008C / PMA1

Click on Significant Values for Screen Details ID:SGTC_44|Compound:0929-0055|FD-Score:4.08|P-value:3.92E-6 ID:SGTC_50|Compound:0929-0078|FD-Score:7.70|P-value:1.72E-17 ID:SGTC_70|Compound:0141-0289|FD-Score:3.40|P-value:9.78E-5 ID:SGTC_124|Compound:0136-0217|FD-Score:4.80|P-value:7.20E-8 ID:SGTC_129|Compound:0088-0017|FD-Score:3.81|P-value:1.49E-5 ID:SGTC_131|Compound:0141-0277|FD-Score:2.94|P-value:6.35E-4 ID:SGTC_153|Compound:4077-0274|FD-Score:3.18|P-value:2.42E-4 ID:SGTC_189|Compound:3970-0742|FD-Score:2.87|P-value:8.31E-4 ID:SGTC_190|Compound:3970-0790|FD-Score:3.09|P-value:3.44E-4 ID:SGTC_204|Compound:1543-0184|FD-Score:19.50|P-value:1.57E-101 ID:SGTC_298|Compound:1391-0729|FD-Score:-2.88|P-value:8.37E-4 ID:SGTC_528|Compound:3492-0019|FD-Score:-3.18|P-value:2.62E-4 ID:SGTC_614|Compound:0462-0418|FD-Score:-2.95|P-value:6.39E-4 ID:SGTC_767|Compound:0929-0023|FD-Score:10.60|P-value:2.94E-31 ID:SGTC_796|Compound:1234-0020|FD-Score:-3.79|P-value:1.74E-5 ID:SGTC_807|Compound:1315-0151|FD-Score:3.01|P-value:4.76E-4 ID:SGTC_819|Compound:1013-0258|FD-Score:3.56|P-value:4.71E-5 ID:SGTC_892|Compound:0929-0076|FD-Score:7.52|P-value:8.69E-17 ID:SGTC_941|Compound:3237-0776|FD-Score:3.76|P-value:1.85E-5 ID:SGTC_994|Compound:3910-0338|FD-Score:2.89|P-value:7.49E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-4.41|P-value:7.34E-7 ID:SGTC_1131|Compound:3454-3128|FD-Score:-3.64|P-value:3.62E-5 ID:SGTC_1213|Compound:1315-0166|FD-Score:-3.39|P-value:1.07E-4 ID:SGTC_1551|Compound:6-bromo-3'-methylflavone|FD-Score:2.94|P-value:6.22E-4 ID:SGTC_1564|Compound:D-Arginine|FD-Score:-3.34|P-value:1.35E-4 ID:SGTC_1567|Compound:st069303|FD-Score:-3.36|P-value:1.21E-4 ID:SGTC_1611|Compound:st001856|FD-Score:3.32|P-value:1.36E-4 ID:SGTC_1712|Compound:st032288|FD-Score:-3.07|P-value:4.11E-4 ID:SGTC_1750|Compound:st081859|FD-Score:5.71|P-value:2.02E-10 ID:SGTC_1881|Compound:5652484|FD-Score:3.55|P-value:4.87E-5 ID:SGTC_1883|Compound:5-benzylidenerhodanine|FD-Score:5.81|P-value:1.01E-10 ID:SGTC_1915|Compound:st067105|FD-Score:-2.90|P-value:7.89E-4 ID:SGTC_1918|Compound:oxolan-2-ylmethyl 3-bromobenzoate|FD-Score:3.04|P-value:4.20E-4 ID:SGTC_1921|Compound:5740136|FD-Score:7.66|P-value:2.35E-17 ID:SGTC_1922|Compound:5578187|FD-Score:6.98|P-value:1.08E-14 ID:SGTC_1968|Compound:st069590|FD-Score:3.07|P-value:3.85E-4 ID:SGTC_1997|Compound:st073024|FD-Score:2.83|P-value:9.41E-4 ID:SGTC_2103|Compound:5378138|FD-Score:2.92|P-value:6.70E-4 ID:SGTC_2107|Compound:5363541|FD-Score:2.87|P-value:8.16E-4 ID:SGTC_2248|Compound:tofa|FD-Score:5.94|P-value:3.90E-11 ID:SGTC_2309|Compound:7664088|FD-Score:4.06|P-value:4.35E-6 ID:SGTC_2314|Compound:7727897|FD-Score:-3.36|P-value:1.24E-4 ID:SGTC_2365|Compound:9042475|FD-Score:-3.44|P-value:8.55E-5 ID:SGTC_2447|Compound:5741742|FD-Score:4.20|P-value:2.08E-6 ID:SGTC_2515|Compound:chrysin|FD-Score:4.37|P-value:8.54E-7 ID:SGTC_2566|Compound:crystal violet carbinol|FD-Score:-3.27|P-value:1.79E-4 ID:SGTC_2853|Compound:9015042|FD-Score:-3.04|P-value:4.62E-4 ID:SGTC_44|Compound:0929-0055|FD-Score:4.08|P-value:3.92E-6 ID:SGTC_50|Compound:0929-0078|FD-Score:7.70|P-value:1.72E-17 ID:SGTC_70|Compound:0141-0289|FD-Score:3.40|P-value:9.78E-5 ID:SGTC_124|Compound:0136-0217|FD-Score:4.80|P-value:7.20E-8 ID:SGTC_129|Compound:0088-0017|FD-Score:3.81|P-value:1.49E-5 ID:SGTC_131|Compound:0141-0277|FD-Score:2.94|P-value:6.35E-4 ID:SGTC_153|Compound:4077-0274|FD-Score:3.18|P-value:2.42E-4 ID:SGTC_189|Compound:3970-0742|FD-Score:2.87|P-value:8.31E-4 ID:SGTC_190|Compound:3970-0790|FD-Score:3.09|P-value:3.44E-4 ID:SGTC_204|Compound:1543-0184|FD-Score:19.50|P-value:1.57E-101 ID:SGTC_298|Compound:1391-0729|FD-Score:-2.88|P-value:8.37E-4 ID:SGTC_528|Compound:3492-0019|FD-Score:-3.18|P-value:2.62E-4 ID:SGTC_614|Compound:0462-0418|FD-Score:-2.95|P-value:6.39E-4 ID:SGTC_767|Compound:0929-0023|FD-Score:10.60|P-value:2.94E-31 ID:SGTC_796|Compound:1234-0020|FD-Score:-3.79|P-value:1.74E-5 ID:SGTC_807|Compound:1315-0151|FD-Score:3.01|P-value:4.76E-4 ID:SGTC_819|Compound:1013-0258|FD-Score:3.56|P-value:4.71E-5 ID:SGTC_892|Compound:0929-0076|FD-Score:7.52|P-value:8.69E-17 ID:SGTC_941|Compound:3237-0776|FD-Score:3.76|P-value:1.85E-5 ID:SGTC_994|Compound:3910-0338|FD-Score:2.89|P-value:7.49E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-4.41|P-value:7.34E-7 ID:SGTC_1131|Compound:3454-3128|FD-Score:-3.64|P-value:3.62E-5 ID:SGTC_1213|Compound:1315-0166|FD-Score:-3.39|P-value:1.07E-4 ID:SGTC_1551|Compound:6-bromo-3'-methylflavone|FD-Score:2.94|P-value:6.22E-4 ID:SGTC_1564|Compound:D-Arginine|FD-Score:-3.34|P-value:1.35E-4 ID:SGTC_1567|Compound:st069303|FD-Score:-3.36|P-value:1.21E-4 ID:SGTC_1611|Compound:st001856|FD-Score:3.32|P-value:1.36E-4 ID:SGTC_1712|Compound:st032288|FD-Score:-3.07|P-value:4.11E-4 ID:SGTC_1750|Compound:st081859|FD-Score:5.71|P-value:2.02E-10 ID:SGTC_1881|Compound:5652484|FD-Score:3.55|P-value:4.87E-5 ID:SGTC_1883|Compound:5-benzylidenerhodanine|FD-Score:5.81|P-value:1.01E-10 ID:SGTC_1915|Compound:st067105|FD-Score:-2.90|P-value:7.89E-4 ID:SGTC_1918|Compound:oxolan-2-ylmethyl 3-bromobenzoate|FD-Score:3.04|P-value:4.20E-4 ID:SGTC_1921|Compound:5740136|FD-Score:7.66|P-value:2.35E-17 ID:SGTC_1922|Compound:5578187|FD-Score:6.98|P-value:1.08E-14 ID:SGTC_1968|Compound:st069590|FD-Score:3.07|P-value:3.85E-4 ID:SGTC_1997|Compound:st073024|FD-Score:2.83|P-value:9.41E-4 ID:SGTC_2103|Compound:5378138|FD-Score:2.92|P-value:6.70E-4 ID:SGTC_2107|Compound:5363541|FD-Score:2.87|P-value:8.16E-4 ID:SGTC_2248|Compound:tofa|FD-Score:5.94|P-value:3.90E-11 ID:SGTC_2309|Compound:7664088|FD-Score:4.06|P-value:4.35E-6 ID:SGTC_2314|Compound:7727897|FD-Score:-3.36|P-value:1.24E-4 ID:SGTC_2365|Compound:9042475|FD-Score:-3.44|P-value:8.55E-5 ID:SGTC_2447|Compound:5741742|FD-Score:4.20|P-value:2.08E-6 ID:SGTC_2515|Compound:chrysin|FD-Score:4.37|P-value:8.54E-7 ID:SGTC_2566|Compound:crystal violet carbinol|FD-Score:-3.27|P-value:1.79E-4 ID:SGTC_2853|Compound:9015042|FD-Score:-3.04|P-value:4.62E-4

Top fitness defect scores for YGL008C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_204 1543-0184 19.50 62.4 µM 1.57E-101 TRP & mitochondrial translation 1 4.09E-85 9.31
2 SGTC_767 0929-0023 10.60 86.0 µM 2.94E-31 TRP & mitochondrial translation 2 2.13E-26 3.93
3 SGTC_50 0929-0078 7.70 56.4 µM 1.72E-17 1 6.96E-15 2.23
4 SGTC_1921 5740136 7.66 50.0 µM 2.35E-17 TRP & mitochondrial translation 2 9.03E-15 1.33
5 SGTC_892 0929-0076 7.52 29.0 µM 8.69E-17 TRP & mitochondrial translation 1 2.69E-14 1.35
6 SGTC_1922 5578187 6.98 25.0 µM 1.08E-14 TRP & mitochondrial translation 2 1.52E-12 1.57
7 SGTC_2248 tofa 5.94 1.1 µM 3.90E-11 sphingolipid biosynthesis & PDR1 16 1.45E-9 0.21
8 SGTC_1883 5-benzylidenerhodanine 5.81 20.0 µM 1.01E-10 TRP & mitochondrial translation 4 3.21E-9 1.38
9 SGTC_1750 st081859 5.71 2.4 µM 2.02E-10 endomembrane recycling 17 5.77E-9 0.43
10 SGTC_124 0136-0217 4.80 7.1 µM 7.20E-8 mitochondrial stress 16 7.95E-7 0.22
11 SGTC_2515 chrysin 4.37 20.8 µM 8.54E-7 RPP1 & pyrimidine depletion 3 6.34E-6 0.09
12 SGTC_2447 5741742 4.20 33.8 µM 2.08E-6 8 1.34E-5 0.46
13 SGTC_44 0929-0055 4.08 61.2 µM 3.92E-6 7 2.28E-5 0.07
14 SGTC_2309 7664088 4.06 163.9 µM 4.35E-6 10 2.49E-5 0.06
15 SGTC_129 0088-0017 3.81 176.6 µM 1.49E-5 6 7.01E-5 0.04
16 SGTC_941 3237-0776 3.76 460.0 µM 1.85E-5 5 8.42E-5 0.11
17 SGTC_819 1013-0258 3.56 27.7 µM 4.71E-5 6 1.85E-4 0.13
18 SGTC_1881 5652484 3.55 16.0 µM 4.87E-5 TRP & mitochondrial translation 10 1.90E-4 0.01
19 SGTC_70 0141-0289 3.40 43.3 µM 9.78E-5 mitochondrial stress 27 3.42E-4 0.12
20 SGTC_1611 st001856 3.32 99.4 µM 1.36E-4 17 4.52E-4 0.04

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.450 1.00E-166 YGL007W_d BRP1_d hom Dubious ORF located in the upstream region of PMA1, deletion leads to polyamine resistance due to downregulation of PMA1
0.308 1.58E-74 YKL077W_p YKL077W_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole
0.216 8.27E-37 YDR203W_d YDR203W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.210 1.06E-34 YBR002C RER2 het Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting
0.203 1.33E-32 YDR414C ERD1 hom Predicted membrane protein required for the retention of lumenal endoplasmic reticulum proteins; mutants secrete the endogenous ER protein, BiP (Kar2p)
0.203 1.58E-32 YHR098C SFB3 hom Component of the Sec23p-Sfb3p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb2p
0.173 4.69E-24 YBL090W MRP21 hom Mitochondrial ribosomal protein of the small subunit; MRP21 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences
0.161 6.28E-21 YDR166C SEC5 het Essential 107kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in assembly of the exocyst complex; required with Sec3p for ER inheritance where it promotes anchoring of the cortical ER at the bud tip
0.155 1.77E-19 YNL148C ALF1 hom Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance
0.155 2.09E-19 YGR037C ACB1 hom Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress
0.154 2.97E-19 YDL193W NUS1 het Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY
0.153 5.13E-19 YFR009W GCN20 hom Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA
0.150 2.28E-18 YOR162C YRR1 hom Zn2-Cys6 zinc-finger transcription factor; activates genes involved in multidrug resistance; paralog of Yrm1p, acting on an overlapping set of target genes; YRR1 has a paralog, PDR8, that arose from the whole genome duplication
0.148 7.03E-18 YBR158W AMN1 hom Protein required for daughter cell separation, multiple mitotic checkpoints, and chromosome stability; contains 12 degenerate leucine-rich repeat motifs; expression is induced by the Mitotic Exit Network (MEN)
0.147 1.19E-17 YGL026C TRP5 hom Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis