YGL009C / LEU1

Isopropylmalate isomerase, catalyzes the second step in the leucine biosynthesis pathway

Zygosity: Homozygous strain
fixedexpanded
Profile for YGL009C / LEU1

Click on Significant Values for Screen Details ID:SGTC_634|Compound:1611-4317|FD-Score:3.66|P-value:1.10E-4 ID:SGTC_648|Compound:0375-0473|FD-Score:3.51|P-value:1.91E-4 ID:SGTC_815|Compound:1582-0027|FD-Score:3.56|P-value:1.62E-4 ID:SGTC_945|Compound:3381-0768|FD-Score:3.77|P-value:6.87E-5 ID:SGTC_946|Compound:3434-1334|FD-Score:-3.28|P-value:4.45E-4 ID:SGTC_1179|Compound:1021-1431|FD-Score:4.05|P-value:2.13E-5 ID:SGTC_1452|Compound:4194-0072|FD-Score:3.69|P-value:9.72E-5 ID:SGTC_1563|Compound:conessine|FD-Score:3.13|P-value:7.68E-4 ID:SGTC_1566|Compound:6,7-dimethoxy-4-ethylcoumarin|FD-Score:3.29|P-value:4.40E-4 ID:SGTC_1880|Compound:MG-132|FD-Score:4.64|P-value:1.39E-6 ID:SGTC_2466|Compound:5983409|FD-Score:-3.80|P-value:5.92E-5 ID:SGTC_2469|Compound:5655019|FD-Score:-3.89|P-value:4.07E-5 ID:SGTC_2471|Compound:5466956|FD-Score:3.25|P-value:5.14E-4 ID:SGTC_2476|Compound:5806916|FD-Score:-3.71|P-value:8.66E-5 ID:SGTC_2551|Compound:apiole|FD-Score:3.40|P-value:2.89E-4 ID:SGTC_2580|Compound:diallyl disulphide|FD-Score:3.07|P-value:9.67E-4 ID:SGTC_2581|Compound:avocatin a|FD-Score:-4.09|P-value:1.70E-5 ID:SGTC_2590|Compound:3,4',5',6',7'-pentamethoxyflavone|FD-Score:3.35|P-value:3.55E-4 ID:SGTC_2709|Compound:st078560|FD-Score:3.96|P-value:3.11E-5 ID:SGTC_2775|Compound:cyproheptadine|FD-Score:-3.93|P-value:3.46E-5 ID:SGTC_2817|Compound:7995342|FD-Score:7.57|P-value:9.52E-15 ID:SGTC_2819|Compound:7996655|FD-Score:-3.33|P-value:3.69E-4 ID:SGTC_2821|Compound:7996764|FD-Score:4.58|P-value:1.85E-6 ID:SGTC_2884|Compound:9058502|FD-Score:-3.14|P-value:7.50E-4 ID:SGTC_2917|Compound:7979103|FD-Score:4.13|P-value:1.47E-5 ID:SGTC_2987|Compound:9018338|FD-Score:3.07|P-value:9.51E-4 ID:SGTC_634|Compound:1611-4317|FD-Score:3.66|P-value:1.10E-4 ID:SGTC_648|Compound:0375-0473|FD-Score:3.51|P-value:1.91E-4 ID:SGTC_815|Compound:1582-0027|FD-Score:3.56|P-value:1.62E-4 ID:SGTC_945|Compound:3381-0768|FD-Score:3.77|P-value:6.87E-5 ID:SGTC_946|Compound:3434-1334|FD-Score:-3.28|P-value:4.45E-4 ID:SGTC_1179|Compound:1021-1431|FD-Score:4.05|P-value:2.13E-5 ID:SGTC_1452|Compound:4194-0072|FD-Score:3.69|P-value:9.72E-5 ID:SGTC_1563|Compound:conessine|FD-Score:3.13|P-value:7.68E-4 ID:SGTC_1566|Compound:6,7-dimethoxy-4-ethylcoumarin|FD-Score:3.29|P-value:4.40E-4 ID:SGTC_1880|Compound:MG-132|FD-Score:4.64|P-value:1.39E-6 ID:SGTC_2466|Compound:5983409|FD-Score:-3.80|P-value:5.92E-5 ID:SGTC_2469|Compound:5655019|FD-Score:-3.89|P-value:4.07E-5 ID:SGTC_2471|Compound:5466956|FD-Score:3.25|P-value:5.14E-4 ID:SGTC_2476|Compound:5806916|FD-Score:-3.71|P-value:8.66E-5 ID:SGTC_2551|Compound:apiole|FD-Score:3.40|P-value:2.89E-4 ID:SGTC_2580|Compound:diallyl disulphide|FD-Score:3.07|P-value:9.67E-4 ID:SGTC_2581|Compound:avocatin a|FD-Score:-4.09|P-value:1.70E-5 ID:SGTC_2590|Compound:3,4',5',6',7'-pentamethoxyflavone|FD-Score:3.35|P-value:3.55E-4 ID:SGTC_2709|Compound:st078560|FD-Score:3.96|P-value:3.11E-5 ID:SGTC_2775|Compound:cyproheptadine|FD-Score:-3.93|P-value:3.46E-5 ID:SGTC_2817|Compound:7995342|FD-Score:7.57|P-value:9.52E-15 ID:SGTC_2819|Compound:7996655|FD-Score:-3.33|P-value:3.69E-4 ID:SGTC_2821|Compound:7996764|FD-Score:4.58|P-value:1.85E-6 ID:SGTC_2884|Compound:9058502|FD-Score:-3.14|P-value:7.50E-4 ID:SGTC_2917|Compound:7979103|FD-Score:4.13|P-value:1.47E-5 ID:SGTC_2987|Compound:9018338|FD-Score:3.07|P-value:9.51E-4

Top fitness defect scores for YGL009C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2817 7995342 7.57 71.4 µM 9.52E-15 1 1.84E-14
2 SGTC_1880 MG-132 4.64 525.0 µM 1.39E-6 10 1.77E-6
3 SGTC_2821 7996764 4.58 71.4 µM 1.85E-6 22 2.35E-6
4 SGTC_2917 7979103 4.13 53.8 µM 1.47E-5 25 1.79E-5
5 SGTC_1179 1021-1431 4.05 9.3 µM 2.13E-5 40 2.57E-5
6 SGTC_2709 st078560 3.96 56.0 µM 3.11E-5 11 3.73E-5
7 SGTC_945 3381-0768 3.77 11.3 µM 6.87E-5 endomembrane recycling 37 8.10E-5
8 SGTC_1452 4194-0072 3.69 190.0 µM 9.72E-5 16 1.14E-4
9 SGTC_634 1611-4317 3.66 11.2 µM 1.10E-4 ERG2 22 1.29E-4
10 SGTC_815 1582-0027 3.56 36.9 µM 1.62E-4 44 1.88E-4
11 SGTC_648 0375-0473 3.51 33.3 µM 1.91E-4 28 2.20E-4
12 SGTC_2551 apiole 3.40 73.9 µM 2.89E-4 16 3.31E-4
13 SGTC_2590 3,4',5',6',7'-pentamethoxyflavone 3.35 100.0 µM 3.55E-4 60S ribosome export 45 4.04E-4
14 SGTC_1566 6,7-dimethoxy-4-ethylcoumarin 3.29 85.4 µM 4.40E-4 43 4.99E-4
15 SGTC_2471 5466956 3.25 24.8 µM 5.14E-4 mitochondrial processes 30 5.82E-4
16 SGTC_1563 conessine 3.13 56.1 µM 7.68E-4 43 8.61E-4
17 SGTC_2987 9018338 3.07 71.4 µM 9.51E-4 RPP1 & pyrimidine depletion 66 0.00106
18 SGTC_2580 diallyl disulphide 3.07 81.4 µM 9.67E-4 57 0.00108
19 SGTC_1572 epiandrosterone 3.05 68.9 µM 0.00101 58 0.00113
20 SGTC_1393 3470-5649 3.05 58.2 µM 0.00101 60S ribosome export 36 0.00113

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.138 1.04E-15 YLR306W UBC12 hom Enzyme that mediates the conjugation of Rub1p, a ubiquitin-like protein, to other proteins; related to E2 ubiquitin-conjugating enzymes
0.112 7.65E-11 YKL154W SRP102 het Signal recognition particle (SRP) receptor beta subunit; involved in SRP-dependent protein targeting; anchors the alpha subunit, Srp101p to the ER membrane
0.109 2.58E-10 YOR316C-A_p YOR316C-A_p hom Putative protein of unknown function; identified by fungal homology and RT-PCR
0.104 1.74E-9 YBR146W MRPS9 hom Mitochondrial ribosomal protein of the small subunit
0.103 2.06E-9 YLR281C_p YLR281C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YLR281C is not an essential gene
0.102 2.71E-9 YGR201C_p YGR201C_p hom Putative protein of unknown function
0.102 3.85E-9 YMR173W DDR48 hom DNA damage-responsive protein; expression is increased in response to heat-shock stress or treatments that produce DNA lesions; contains multiple repeats of the amino acid sequence NNNDSYGS; protein abundance increases in response to DNA replication stress
0.094 4.40E-8 YMR116C ASC1 hom G-protein beta subunit and guanine nucleotide dissociation inhibitor for Gpa2p; ortholog of RACK1 that inhibits translation; core component of the small (40S) ribosomal subunit; regulates P-body formation induced by replication stress; represses Gcn4p in the absence of amino acid starvation
0.094 4.83E-8 YBR089W_d YBR089W_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps the verified gene POL30
0.092 8.96E-8 YLR094C GIS3 hom Protein of unknown function
0.091 1.34E-7 YMR147W_p YMR147W_p hom Putative protein of unknown function
0.090 1.57E-7 YNL010W_p YNL010W_p hom Putative protein of unknown function with similarity to phosphoserine phosphatases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; homozygous diploid mutant shows an increase in glycogen accumulation
0.090 1.75E-7 YKR035W-A DID2 hom Class E protein of the vacuolar protein-sorting (Vps) pathway; binds Vps4p and directs it to dissociate ESCRT-III complexes; forms a functional and physical complex with Ist1p; human ortholog may be altered in breast tumors
0.086 6.59E-7 YDR086C SSS1 het Subunit of the Sec61p translocation complex (Sec61p-Sss1p-Sbh1p) that forms a channel for passage of secretory proteins through the endoplasmic reticulum membrane, and of the Ssh1p complex (Ssh1p-Sbh2p-Sss1p); interacts with Ost4p and Wbp1p
0.086 7.09E-7 YLL025W PAU17 hom Protein of unknown function, member of the seripauperin multigene family encoded mainly in subtelomeric regions; YLL025W is not an essential gene