YGL014W / PUF4

Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding nucleolar ribosomal RNA-processing factors

Zygosity: Homozygous strain
fixedexpanded
Profile for YGL014W / PUF4

Click on Significant Values for Screen Details ID:SGTC_14|Compound:0354-0009|FD-Score:3.90|P-value:2.37E-4 ID:SGTC_140|Compound:0062-0056|FD-Score:4.38|P-value:4.38E-5 ID:SGTC_324|Compound:1315-0088|FD-Score:-3.66|P-value:4.89E-4 ID:SGTC_327|Compound:1273-0057|FD-Score:-3.80|P-value:3.12E-4 ID:SGTC_572|Compound:0960-0087|FD-Score:3.62|P-value:5.95E-4 ID:SGTC_588|Compound:3970-1144|FD-Score:3.61|P-value:6.15E-4 ID:SGTC_677|Compound:0929-0063|FD-Score:3.74|P-value:4.03E-4 ID:SGTC_715|Compound:0868-0130|FD-Score:-4.36|P-value:4.21E-5 ID:SGTC_753|Compound:1322-0200|FD-Score:3.61|P-value:6.15E-4 ID:SGTC_787|Compound:4152-0075|FD-Score:4.21|P-value:8.07E-5 ID:SGTC_795|Compound:0685-0265|FD-Score:4.04|P-value:1.49E-4 ID:SGTC_815|Compound:1582-0027|FD-Score:4.04|P-value:1.49E-4 ID:SGTC_1016|Compound:4226-1401|FD-Score:3.97|P-value:1.88E-4 ID:SGTC_1026|Compound:k007-1105|FD-Score:4.99|P-value:3.83E-6 ID:SGTC_1366|Compound:1683-0113|FD-Score:5.85|P-value:7.73E-8 ID:SGTC_1463|Compound:k081-0032|FD-Score:3.69|P-value:4.82E-4 ID:SGTC_1579|Compound:chlormadinone acetate|FD-Score:5.25|P-value:1.26E-6 ID:SGTC_1727|Compound:st034309|FD-Score:4.38|P-value:4.38E-5 ID:SGTC_1734|Compound:st037168|FD-Score:4.88|P-value:6.14E-6 ID:SGTC_2247|Compound:cerulenin|FD-Score:3.93|P-value:2.18E-4 ID:SGTC_2291|Compound:7917025|FD-Score:3.77|P-value:3.70E-4 ID:SGTC_2330|Compound:7979373|FD-Score:4.60|P-value:1.88E-5 ID:SGTC_2353|Compound:9059630|FD-Score:-3.58|P-value:6.31E-4 ID:SGTC_2388|Compound:9080168|FD-Score:3.61|P-value:6.07E-4 ID:SGTC_2442|Compound:5158906|FD-Score:4.12|P-value:1.11E-4 ID:SGTC_2448|Compound:5185112|FD-Score:3.72|P-value:4.27E-4 ID:SGTC_2467|Compound:5474540|FD-Score:4.29|P-value:6.06E-5 ID:SGTC_2594|Compound:a-mangostin|FD-Score:-4.33|P-value:4.87E-5 ID:SGTC_2706|Compound:st078239|FD-Score:3.61|P-value:6.17E-4 ID:SGTC_2786|Compound:7743617|FD-Score:4.74|P-value:1.06E-5 ID:SGTC_2896|Compound:9052656|FD-Score:4.45|P-value:3.39E-5 ID:SGTC_3058|Compound:9093861|FD-Score:-3.89|P-value:2.25E-4 ID:SGTC_3144|Compound:9087057|FD-Score:-5.38|P-value:6.31E-7 ID:SGTC_3195|Compound:9113082|FD-Score:-4.15|P-value:9.27E-5 ID:SGTC_3268|Compound:9137683|FD-Score:3.61|P-value:6.06E-4 ID:SGTC_14|Compound:0354-0009|FD-Score:3.90|P-value:2.37E-4 ID:SGTC_140|Compound:0062-0056|FD-Score:4.38|P-value:4.38E-5 ID:SGTC_324|Compound:1315-0088|FD-Score:-3.66|P-value:4.89E-4 ID:SGTC_327|Compound:1273-0057|FD-Score:-3.80|P-value:3.12E-4 ID:SGTC_572|Compound:0960-0087|FD-Score:3.62|P-value:5.95E-4 ID:SGTC_588|Compound:3970-1144|FD-Score:3.61|P-value:6.15E-4 ID:SGTC_677|Compound:0929-0063|FD-Score:3.74|P-value:4.03E-4 ID:SGTC_715|Compound:0868-0130|FD-Score:-4.36|P-value:4.21E-5 ID:SGTC_753|Compound:1322-0200|FD-Score:3.61|P-value:6.15E-4 ID:SGTC_787|Compound:4152-0075|FD-Score:4.21|P-value:8.07E-5 ID:SGTC_795|Compound:0685-0265|FD-Score:4.04|P-value:1.49E-4 ID:SGTC_815|Compound:1582-0027|FD-Score:4.04|P-value:1.49E-4 ID:SGTC_1016|Compound:4226-1401|FD-Score:3.97|P-value:1.88E-4 ID:SGTC_1026|Compound:k007-1105|FD-Score:4.99|P-value:3.83E-6 ID:SGTC_1366|Compound:1683-0113|FD-Score:5.85|P-value:7.73E-8 ID:SGTC_1463|Compound:k081-0032|FD-Score:3.69|P-value:4.82E-4 ID:SGTC_1579|Compound:chlormadinone acetate|FD-Score:5.25|P-value:1.26E-6 ID:SGTC_1727|Compound:st034309|FD-Score:4.38|P-value:4.38E-5 ID:SGTC_1734|Compound:st037168|FD-Score:4.88|P-value:6.14E-6 ID:SGTC_2247|Compound:cerulenin|FD-Score:3.93|P-value:2.18E-4 ID:SGTC_2291|Compound:7917025|FD-Score:3.77|P-value:3.70E-4 ID:SGTC_2330|Compound:7979373|FD-Score:4.60|P-value:1.88E-5 ID:SGTC_2353|Compound:9059630|FD-Score:-3.58|P-value:6.31E-4 ID:SGTC_2388|Compound:9080168|FD-Score:3.61|P-value:6.07E-4 ID:SGTC_2442|Compound:5158906|FD-Score:4.12|P-value:1.11E-4 ID:SGTC_2448|Compound:5185112|FD-Score:3.72|P-value:4.27E-4 ID:SGTC_2467|Compound:5474540|FD-Score:4.29|P-value:6.06E-5 ID:SGTC_2594|Compound:a-mangostin|FD-Score:-4.33|P-value:4.87E-5 ID:SGTC_2706|Compound:st078239|FD-Score:3.61|P-value:6.17E-4 ID:SGTC_2786|Compound:7743617|FD-Score:4.74|P-value:1.06E-5 ID:SGTC_2896|Compound:9052656|FD-Score:4.45|P-value:3.39E-5 ID:SGTC_3058|Compound:9093861|FD-Score:-3.89|P-value:2.25E-4 ID:SGTC_3144|Compound:9087057|FD-Score:-5.38|P-value:6.31E-7 ID:SGTC_3195|Compound:9113082|FD-Score:-4.15|P-value:9.27E-5 ID:SGTC_3268|Compound:9137683|FD-Score:3.61|P-value:6.06E-4

Top fitness defect scores for YGL014W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1366 1683-0113 5.85 21.9 µM 7.73E-8 2 2.42E-9
2 SGTC_1579 chlormadinone acetate 5.25 49.4 µM 1.26E-6 2 7.58E-8
3 SGTC_1026 k007-1105 4.99 30.6 µM 3.83E-6 ERAD & cell cycle 59 2.98E-7
4 SGTC_1734 st037168 4.88 69.4 µM 6.14E-6 9 5.32E-7
5 SGTC_2786 7743617 4.74 45.5 µM 1.06E-5 12 1.05E-6
6 SGTC_2330 7979373 4.60 200.0 µM 1.88E-5 60S ribosome export 21 2.11E-6
7 SGTC_2896 9052656 4.45 71.4 µM 3.39E-5 endomembrane recycling 85 4.36E-6
8 SGTC_1727 st034309 4.38 9.9 µM 4.38E-5 25 5.98E-6
9 SGTC_140 0062-0056 4.38 502.5 µM 4.38E-5 15 5.98E-6
10 SGTC_2467 5474540 4.29 200.0 µM 6.06E-5 11 8.91E-6
11 SGTC_787 4152-0075 4.21 138.0 µM 8.07E-5 21 1.27E-5
12 SGTC_2442 5158906 4.12 48.5 µM 1.11E-4 16 1.88E-5
13 SGTC_795 0685-0265 4.04 78.0 µM 1.49E-4 excess fatty acid 22 2.69E-5
14 SGTC_815 1582-0027 4.04 36.9 µM 1.49E-4 23 2.70E-5
15 SGTC_1016 4226-1401 3.97 38.0 µM 1.88E-4 RNA pol III & RNase P/MRP 20 3.58E-5
16 SGTC_2247 cerulenin 3.93 830.0 nM 2.18E-4 18 4.29E-5
17 SGTC_14 0354-0009 3.90 295.0 µM 2.37E-4 29 4.76E-5
18 SGTC_2291 7917025 3.77 104.3 µM 3.70E-4 iron homeostasis 56 8.21E-5
19 SGTC_677 0929-0063 3.74 136.0 µM 4.03E-4 19 9.12E-5
20 SGTC_2448 5185112 3.72 27.2 µM 4.27E-4 15 9.80E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.102 2.92E-9 YMR058W FET3 hom Ferro-O2-oxidoreductase; required for high-affinity iron uptake and involved in mediating resistance to copper ion toxicity, belongs to class of integral membrane multicopper oxidases; protein abundance increases in response to DNA replication stress
0.085 9.36E-7 YDL095W PMT1 hom Protein O-mannosyltransferase of the ER membrane; transfers mannose from dolichyl phosphate-D-mannose to protein serine and threonine residues; 1 of 7 related proteins involved in O-glycosylation which is essential for cell wall rigidity; involved in ER quality control; amino terminus faces cytoplasm, carboxyl terminus faces ER lumen
0.080 3.25E-6 YLR031W_p YLR031W_p hom Putative protein of unknown function
0.075 1.24E-5 YMR057C_d YMR057C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF AAC1
0.074 1.93E-5 YOL030W GAS5 hom 1,3-beta-glucanosyltransferase, has similarity to Gas1p; localizes to the cell wall
0.073 2.20E-5 YBR014C GRX7 hom Cis-golgi localized monothiol glutaredoxin; more similar in activity to dithiol than other monothiol glutaredoxins; involved in the oxidative stress response; does not bind metal ions; GRX7 has a paralog, GRX6, that arose from the whole genome duplication
0.072 3.23E-5 YDR451C YHP1 hom Homeobox transcriptional repressor; binds Mcm1p and early cell cycle box (ECB) elements of cell cycle regulated genes, thereby restricting ECB-mediated transcription to the M/G1 interval; YHP1 has a paralog, YOX1, that arose from the whole genome duplication
0.071 3.47E-5 YOR310C NOP58 het Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the small subunit processome complex, which is required for processing of pre-18S rRNA
0.070 5.10E-5 YDL096C_d OPI6_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene PMT1/YDL095W; YDL096C is not essential
0.070 5.32E-5 YNL155W YNL155W hom Protein of unknown function; contains a DHHC domain and an AN1-type zinc finger; predicted to have thiol-disulfide oxidoreductase active site; transcriptionally regulated by Rpn4p along with proteasome subunit genes; putative ortholog of human AIRAP, which stimulates proteasome activity in response to arsenic; protein abundance increases in response to DNA replication stress
0.069 5.85E-5 YMR273C ZDS1 hom Protein with a role in regulating Swe1p-dependent polarized growth; involved in maintaining Cdc55p in the cytoplasm where it promotes mitotic entry; involved in mitotic exit through Cdc14p regulation; interacts with silencing proteins at telomeres; has a role in Bcy1p localization; implicated in mRNA nuclear export; ZDS1 has a paralog, ZDS2, that arose from the whole genome duplication
0.068 8.07E-5 YDR021W FAL1 het Nucleolar protein required for maturation of 18S rRNA, member of the eIF4A subfamily of DEAD-box ATP-dependent RNA helicases
0.067 9.27E-5 YBL082C ALG3 hom Dolichol-P-Man dependent alpha(1-3) mannosyltransferase, involved in the synthesis of dolichol-linked oligosaccharide donor for N-linked glycosylation of proteins
0.067 1.09E-4 YEL041W YEF1 hom ATP-NADH kinase; phosphorylates both NAD and NADH; homooctameric structure consisting of 60-kDa subunits; similar to Pos5p; overexpression complements certain pos5 phenotypes; YEF1 has a paralog, UTR1, that arose from the whole genome duplication
0.065 1.75E-4 YGR166W TRS65 hom Subunit of TRAPPII, a multimeric guanine nucleotide-exchange factor for Ypt1p; involved in intra-Golgi traffic and the retrograde pathway from the endosome to Golgi; role in cell wall beta-glucan biosynthesis and the stress response