YGL027C / CWH41

Processing alpha glucosidase I, ER type II integral membrane N-glycoprotein involved in assembly of cell wall beta 1,6 glucan and asparagine-linked protein glycosylation; also involved in ER protein quality control and sensing of ER stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YGL027C / CWH41

Click on Significant Values for Screen Details ID:SGTC_22|Compound:0964-0034|FD-Score:7.93|P-value:2.77E-13 ID:SGTC_58|Compound:0957-0393|FD-Score:-3.49|P-value:7.20E-4 ID:SGTC_121|Compound:0557-0495|FD-Score:4.47|P-value:2.45E-5 ID:SGTC_147|Compound:4466-0538|FD-Score:4.20|P-value:6.88E-5 ID:SGTC_149|Compound:3970-0822|FD-Score:4.11|P-value:9.53E-5 ID:SGTC_150|Compound:3970-0969|FD-Score:-3.58|P-value:5.46E-4 ID:SGTC_247|Compound:mechlorethamine|FD-Score:-3.41|P-value:9.28E-4 ID:SGTC_255|Compound:tunicamycin|FD-Score:3.73|P-value:3.48E-4 ID:SGTC_262|Compound:fenpropimorph|FD-Score:-3.74|P-value:3.26E-4 ID:SGTC_351|Compound:caspofungin|FD-Score:16.30|P-value:3.64E-50 ID:SGTC_531|Compound:3788-1495|FD-Score:4.13|P-value:8.72E-5 ID:SGTC_880|Compound:0833-0487|FD-Score:3.44|P-value:8.94E-4 ID:SGTC_951|Compound:1171-0579|FD-Score:6.66|P-value:7.29E-10 ID:SGTC_999|Compound:4022-0066|FD-Score:4.05|P-value:1.19E-4 ID:SGTC_1033|Compound:k072-0102|FD-Score:4.10|P-value:9.71E-5 ID:SGTC_1035|Compound:k081-0041|FD-Score:-4.58|P-value:1.50E-5 ID:SGTC_1042|Compound:0350-0096|FD-Score:3.65|P-value:4.59E-4 ID:SGTC_1521|Compound:st012942|FD-Score:-3.61|P-value:4.97E-4 ID:SGTC_1623|Compound:st003709|FD-Score:4.17|P-value:7.56E-5 ID:SGTC_1745|Compound:st081495|FD-Score:3.44|P-value:8.95E-4 ID:SGTC_2007|Compound:4011539|FD-Score:4.95|P-value:3.40E-6 ID:SGTC_2016|Compound:5121758|FD-Score:3.91|P-value:1.95E-4 ID:SGTC_2023|Compound:5144124|FD-Score:4.38|P-value:3.45E-5 ID:SGTC_2027|Compound:5150920|FD-Score:-4.04|P-value:1.17E-4 ID:SGTC_2124|Compound:5246320|FD-Score:4.10|P-value:9.78E-5 ID:SGTC_2289|Compound:7946404|FD-Score:5.48|P-value:3.21E-7 ID:SGTC_2399|Compound:5884133|FD-Score:4.44|P-value:2.73E-5 ID:SGTC_2506|Compound:ethyl hematommate|FD-Score:-3.39|P-value:9.98E-4 ID:SGTC_2529|Compound:gangaleoidin|FD-Score:3.65|P-value:4.56E-4 ID:SGTC_2537|Compound:larixol|FD-Score:3.56|P-value:6.07E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:13.40|P-value:1.20E-34 ID:SGTC_2651|Compound:1327-0036|FD-Score:-4.18|P-value:7.06E-5 ID:SGTC_2800|Compound:7887935|FD-Score:3.59|P-value:5.66E-4 ID:SGTC_2946|Compound:9065502|FD-Score:-4.19|P-value:6.74E-5 ID:SGTC_2949|Compound:9072542|FD-Score:-5.60|P-value:1.71E-7 ID:SGTC_2997|Compound:9067075|FD-Score:3.71|P-value:3.77E-4 ID:SGTC_3078|Compound:9116413|FD-Score:7.05|P-value:7.27E-11 ID:SGTC_3212|Compound:9128307|FD-Score:4.46|P-value:2.58E-5 ID:SGTC_3330|Compound:9141424|FD-Score:-4.33|P-value:4.03E-5 ID:SGTC_22|Compound:0964-0034|FD-Score:7.93|P-value:2.77E-13 ID:SGTC_58|Compound:0957-0393|FD-Score:-3.49|P-value:7.20E-4 ID:SGTC_121|Compound:0557-0495|FD-Score:4.47|P-value:2.45E-5 ID:SGTC_147|Compound:4466-0538|FD-Score:4.20|P-value:6.88E-5 ID:SGTC_149|Compound:3970-0822|FD-Score:4.11|P-value:9.53E-5 ID:SGTC_150|Compound:3970-0969|FD-Score:-3.58|P-value:5.46E-4 ID:SGTC_247|Compound:mechlorethamine|FD-Score:-3.41|P-value:9.28E-4 ID:SGTC_255|Compound:tunicamycin|FD-Score:3.73|P-value:3.48E-4 ID:SGTC_262|Compound:fenpropimorph|FD-Score:-3.74|P-value:3.26E-4 ID:SGTC_351|Compound:caspofungin|FD-Score:16.30|P-value:3.64E-50 ID:SGTC_531|Compound:3788-1495|FD-Score:4.13|P-value:8.72E-5 ID:SGTC_880|Compound:0833-0487|FD-Score:3.44|P-value:8.94E-4 ID:SGTC_951|Compound:1171-0579|FD-Score:6.66|P-value:7.29E-10 ID:SGTC_999|Compound:4022-0066|FD-Score:4.05|P-value:1.19E-4 ID:SGTC_1033|Compound:k072-0102|FD-Score:4.10|P-value:9.71E-5 ID:SGTC_1035|Compound:k081-0041|FD-Score:-4.58|P-value:1.50E-5 ID:SGTC_1042|Compound:0350-0096|FD-Score:3.65|P-value:4.59E-4 ID:SGTC_1521|Compound:st012942|FD-Score:-3.61|P-value:4.97E-4 ID:SGTC_1623|Compound:st003709|FD-Score:4.17|P-value:7.56E-5 ID:SGTC_1745|Compound:st081495|FD-Score:3.44|P-value:8.95E-4 ID:SGTC_2007|Compound:4011539|FD-Score:4.95|P-value:3.40E-6 ID:SGTC_2016|Compound:5121758|FD-Score:3.91|P-value:1.95E-4 ID:SGTC_2023|Compound:5144124|FD-Score:4.38|P-value:3.45E-5 ID:SGTC_2027|Compound:5150920|FD-Score:-4.04|P-value:1.17E-4 ID:SGTC_2124|Compound:5246320|FD-Score:4.10|P-value:9.78E-5 ID:SGTC_2289|Compound:7946404|FD-Score:5.48|P-value:3.21E-7 ID:SGTC_2399|Compound:5884133|FD-Score:4.44|P-value:2.73E-5 ID:SGTC_2506|Compound:ethyl hematommate|FD-Score:-3.39|P-value:9.98E-4 ID:SGTC_2529|Compound:gangaleoidin|FD-Score:3.65|P-value:4.56E-4 ID:SGTC_2537|Compound:larixol|FD-Score:3.56|P-value:6.07E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:13.40|P-value:1.20E-34 ID:SGTC_2651|Compound:1327-0036|FD-Score:-4.18|P-value:7.06E-5 ID:SGTC_2800|Compound:7887935|FD-Score:3.59|P-value:5.66E-4 ID:SGTC_2946|Compound:9065502|FD-Score:-4.19|P-value:6.74E-5 ID:SGTC_2949|Compound:9072542|FD-Score:-5.60|P-value:1.71E-7 ID:SGTC_2997|Compound:9067075|FD-Score:3.71|P-value:3.77E-4 ID:SGTC_3078|Compound:9116413|FD-Score:7.05|P-value:7.27E-11 ID:SGTC_3212|Compound:9128307|FD-Score:4.46|P-value:2.58E-5 ID:SGTC_3330|Compound:9141424|FD-Score:-4.33|P-value:4.03E-5

Top fitness defect scores for YGL027C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_351 caspofungin 16.30 25.0 nM 3.64E-50 cell wall 2 3.28E-60
2 SGTC_2582 agaric acid 13.40 100.0 µM 1.20E-34 redox potentiating 25 1.78E-41
3 SGTC_22 0964-0034 7.93 14.5 µM 2.77E-13 unfolded protein response 10 1.07E-15
4 SGTC_3078 9116413 7.05 49.5 µM 7.27E-11 unfolded protein response 6 8.78E-13
5 SGTC_951 1171-0579 6.66 82.7 µM 7.29E-10 cell wall 26 1.41E-11
6 SGTC_2289 7946404 5.48 165.1 µM 3.21E-7 45 2.14E-8
7 SGTC_2007 4011539 4.95 71.8 µM 3.40E-6 cell wall signaling 35 3.65E-7
8 SGTC_121 0557-0495 4.47 61.3 µM 2.45E-5 calcium & mitochondrial duress 43 3.92E-6
9 SGTC_3212 9128307 4.46 49.5 µM 2.58E-5 30 4.15E-6
10 SGTC_2399 5884133 4.44 200.0 µM 2.73E-5 cell wall signaling 53 4.44E-6
11 SGTC_2023 5144124 4.38 124.0 µM 3.45E-5 19 5.90E-6
12 SGTC_147 4466-0538 4.20 29.2 µM 6.88E-5 ergosterol biosynthesis 10 1.35E-5
13 SGTC_1623 st003709 4.17 20.3 µM 7.56E-5 calcium & mitochondrial duress 61 1.51E-5
14 SGTC_531 3788-1495 4.13 174.0 µM 8.72E-5 unfolded protein response 16 1.79E-5
15 SGTC_149 3970-0822 4.11 46.1 µM 9.53E-5 tubulin folding & SWR complex 91 1.99E-5
16 SGTC_1033 k072-0102 4.10 21.3 µM 9.71E-5 60S ribosome export 18 2.04E-5
17 SGTC_2124 5246320 4.10 200.0 µM 9.78E-5 heme biosynthesis & mitochondrial translocase 17 2.05E-5
18 SGTC_999 4022-0066 4.05 22.7 µM 1.19E-4 Golgi 40 2.60E-5
19 SGTC_2016 5121758 3.91 140.0 µM 1.95E-4 redox potentiating 56 4.70E-5
20 SGTC_255 tunicamycin 3.73 200.5 nM 3.48E-4 unfolded protein response 85 9.40E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.299 3.18E-70 YBR229C ROT2 hom Glucosidase II catalytic subunit required for normal cell wall synthesis; mutations in rot2 suppress tor2 mutations, and are synthetically lethal with rot1 mutations
0.256 3.11E-51 YNL027W CRZ1 hom Transcription factor, activates transcription of stress response genes; nuclear localization is positively regulated by calcineurin-mediated dephosphorylation; rapidly localizes to the nucleus under blue light stress
0.235 2.50E-43 YNL106C INP52 hom Polyphosphatidylinositol phosphatase, dephosphorylates a number of phosphatidylinositols (PIs) to PI; involved in endocytosis; hyperosmotic stress causes translocation to actin patches; synaptojanin-like protein with a Sac1 domain
0.217 3.43E-37 YGR196C FYV8 hom Protein of unknown function, required for survival upon exposure to K1 killer toxin
0.214 5.81E-36 YML052W SUR7 hom Plasma membrane protein that localizes to furrow-like invaginations (MCC patches); component of eisosomes; associated with endocytosis, along with Pil1p and Lsp1p; sporulation and plasma membrane sphingolipid content are altered in mutants
0.209 1.69E-34 YOR049C RSB1 hom Suppressor of sphingoid long chain base (LCB) sensitivity of an LCB-lyase mutation; putative integral membrane transporter or flippase that may transport LCBs from the cytoplasmic side toward the extracytoplasmic side of the membrane
0.205 2.69E-33 YBR260C RGD1 hom GTPase-activating protein (RhoGAP) for Rho3p and Rho4p, possibly involved in control of actin cytoskeleton organization
0.201 7.58E-32 YEL036C ANP1 hom Subunit of the alpha-1,6 mannosyltransferase complex; type II membrane protein; has a role in retention of glycosyltransferases in the Golgi; involved in osmotic sensitivity and resistance to aminonitrophenyl propanediol
0.200 1.19E-31 YPL232W SSO1 hom Plasma membrane t-SNARE involved in fusion of secretory vesicles at the plasma membrane and in vesicle fusion during sporulation; forms a complex with Sec9p that binds v-SNARE Snc2p; syntaxin homolog; functionally redundant with Sso2p
0.196 1.67E-30 YNL074C MLF3 hom Serine-rich protein of unknown function, predicted to be palmitoylated; overproduction suppresses the growth inhibition caused by exposure to the immunosuppressant leflunomide
0.191 6.06E-29 YOL003C PFA4 hom Palmitoyltransferase with autoacylation activity, required for palmitoylation of amino acid permeases containing a C-terminal Phe-Trp-Cys site; required for modification of Chs3p; member of the DHHC family of putative palmitoyltransferases
0.188 3.88E-28 YMR109W MYO5 hom One of two type I myosins; contains proline-rich tail homology 2 (TH2) and SH3 domains; MYO5 deletion has little effect on growth, but myo3 myo5 double deletion causes severe defects in growth and actin cytoskeleton organization
0.184 4.38E-27 YOR050C_d YOR050C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutation is viable
0.182 1.94E-26 YLR331C_d JIP3_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene MID2
0.179 1.38E-25 YHR204W MNL1 hom Alpha-1,2-specific exomannosidase of the endoplasmic reticulum; in complex with Pdi1p, generates a Man7GlcNac2 oligosaccharide signal on glycoproteins destined for ubiquitin-proteasome degradation