YGL037C / PNC1

Nicotinamidase that converts nicotinamide to nicotinic acid; part of the NAD(+) salvage pathway; required for life span extension by calorie restriction; PNC1 expression responds to all known stimuli that extend replicative life span; protein increases in abundance and relative distribution to cytoplasmic foci decreases upon DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YGL037C / PNC1

Click on Significant Values for Screen Details ID:SGTC_6|Compound:0844-0013|FD-Score:4.37|P-value:6.38E-7 ID:SGTC_412|Compound:gemfibrozil|FD-Score:-3.65|P-value:2.74E-5 ID:SGTC_656|Compound:4090-1979|FD-Score:3.14|P-value:2.46E-4 ID:SGTC_741|Compound:4582-0002|FD-Score:2.93|P-value:5.73E-4 ID:SGTC_752|Compound:1896-0090|FD-Score:2.91|P-value:6.30E-4 ID:SGTC_781|Compound:4335-2806|FD-Score:2.95|P-value:5.40E-4 ID:SGTC_867|Compound:0518-0118|FD-Score:-2.98|P-value:4.85E-4 ID:SGTC_908|Compound:3448-7491|FD-Score:-3.57|P-value:3.88E-5 ID:SGTC_911|Compound:3456-4043|FD-Score:-2.83|P-value:8.83E-4 ID:SGTC_913|Compound:3487-1245|FD-Score:-3.91|P-value:7.60E-6 ID:SGTC_914|Compound:3820-6779|FD-Score:-3.03|P-value:4.05E-4 ID:SGTC_915|Compound:methyl methanesulfonate (MMS)|FD-Score:3.01|P-value:4.25E-4 ID:SGTC_946|Compound:3434-1334|FD-Score:-3.07|P-value:3.38E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.04|P-value:3.84E-4 ID:SGTC_1262|Compound:0737-0114|FD-Score:2.99|P-value:4.60E-4 ID:SGTC_1351|Compound:1496-0003|FD-Score:-2.90|P-value:6.68E-4 ID:SGTC_1357|Compound:1503-1303|FD-Score:-3.09|P-value:3.20E-4 ID:SGTC_1504|Compound:k297-0033|FD-Score:-3.11|P-value:2.97E-4 ID:SGTC_1598|Compound:3(2'-chlorophenyl)-7-hydroxy-4-phenylcoumarin|FD-Score:-3.35|P-value:1.05E-4 ID:SGTC_1779|Compound:st039427|FD-Score:2.93|P-value:5.66E-4 ID:SGTC_1789|Compound:tunicamycin|FD-Score:-3.19|P-value:2.14E-4 ID:SGTC_2028|Compound:5153890|FD-Score:-2.83|P-value:8.66E-4 ID:SGTC_2030|Compound:5175110|FD-Score:4.59|P-value:1.78E-7 ID:SGTC_2034|Compound:5197015|FD-Score:2.95|P-value:5.32E-4 ID:SGTC_2041|Compound:5107442|FD-Score:-3.00|P-value:4.58E-4 ID:SGTC_2048|Compound:5246248|FD-Score:7.40|P-value:1.20E-16 ID:SGTC_2057|Compound:5236571|FD-Score:3.44|P-value:6.77E-5 ID:SGTC_2084|Compound:5306078|FD-Score:-2.85|P-value:8.10E-4 ID:SGTC_2121|Compound:5257820|FD-Score:2.82|P-value:8.70E-4 ID:SGTC_2355|Compound:9061122|FD-Score:2.80|P-value:9.52E-4 ID:SGTC_2394|Compound:5221648|FD-Score:3.43|P-value:7.08E-5 ID:SGTC_2395|Compound:5646831|FD-Score:3.89|P-value:7.95E-6 ID:SGTC_2436|Compound:5633522|FD-Score:3.12|P-value:2.65E-4 ID:SGTC_2442|Compound:5158906|FD-Score:2.89|P-value:6.72E-4 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:3.33|P-value:1.12E-4 ID:SGTC_2514|Compound:salinomycin|FD-Score:3.85|P-value:9.99E-6 ID:SGTC_2528|Compound:xanthyletin|FD-Score:3.15|P-value:2.33E-4 ID:SGTC_2529|Compound:gangaleoidin|FD-Score:3.10|P-value:2.97E-4 ID:SGTC_2537|Compound:larixol|FD-Score:4.67|P-value:1.13E-7 ID:SGTC_2588|Compound:indole-3-carbinol|FD-Score:-3.99|P-value:5.20E-6 ID:SGTC_2834|Compound:9001755|FD-Score:-5.38|P-value:1.30E-9 ID:SGTC_2862|Compound:9027634|FD-Score:-2.91|P-value:6.44E-4 ID:SGTC_2876|Compound:9059903|FD-Score:4.18|P-value:1.79E-6 ID:SGTC_3201|Compound:9113999|FD-Score:3.61|P-value:3.15E-5 ID:SGTC_3208|Compound:9114115|FD-Score:-3.54|P-value:4.58E-5 ID:SGTC_3248|Compound:9135562|FD-Score:-3.04|P-value:3.90E-4 ID:SGTC_3254|Compound:9136651|FD-Score:-2.98|P-value:4.98E-4 ID:SGTC_3272|Compound:9137413|FD-Score:-2.80|P-value:9.72E-4 ID:SGTC_3323|Compound:9138564|FD-Score:3.33|P-value:1.08E-4 ID:SGTC_3330|Compound:9141424|FD-Score:-3.31|P-value:1.28E-4 ID:SGTC_6|Compound:0844-0013|FD-Score:4.37|P-value:6.38E-7 ID:SGTC_412|Compound:gemfibrozil|FD-Score:-3.65|P-value:2.74E-5 ID:SGTC_656|Compound:4090-1979|FD-Score:3.14|P-value:2.46E-4 ID:SGTC_741|Compound:4582-0002|FD-Score:2.93|P-value:5.73E-4 ID:SGTC_752|Compound:1896-0090|FD-Score:2.91|P-value:6.30E-4 ID:SGTC_781|Compound:4335-2806|FD-Score:2.95|P-value:5.40E-4 ID:SGTC_867|Compound:0518-0118|FD-Score:-2.98|P-value:4.85E-4 ID:SGTC_908|Compound:3448-7491|FD-Score:-3.57|P-value:3.88E-5 ID:SGTC_911|Compound:3456-4043|FD-Score:-2.83|P-value:8.83E-4 ID:SGTC_913|Compound:3487-1245|FD-Score:-3.91|P-value:7.60E-6 ID:SGTC_914|Compound:3820-6779|FD-Score:-3.03|P-value:4.05E-4 ID:SGTC_915|Compound:methyl methanesulfonate (MMS)|FD-Score:3.01|P-value:4.25E-4 ID:SGTC_946|Compound:3434-1334|FD-Score:-3.07|P-value:3.38E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.04|P-value:3.84E-4 ID:SGTC_1262|Compound:0737-0114|FD-Score:2.99|P-value:4.60E-4 ID:SGTC_1351|Compound:1496-0003|FD-Score:-2.90|P-value:6.68E-4 ID:SGTC_1357|Compound:1503-1303|FD-Score:-3.09|P-value:3.20E-4 ID:SGTC_1504|Compound:k297-0033|FD-Score:-3.11|P-value:2.97E-4 ID:SGTC_1598|Compound:3(2'-chlorophenyl)-7-hydroxy-4-phenylcoumarin|FD-Score:-3.35|P-value:1.05E-4 ID:SGTC_1779|Compound:st039427|FD-Score:2.93|P-value:5.66E-4 ID:SGTC_1789|Compound:tunicamycin|FD-Score:-3.19|P-value:2.14E-4 ID:SGTC_2028|Compound:5153890|FD-Score:-2.83|P-value:8.66E-4 ID:SGTC_2030|Compound:5175110|FD-Score:4.59|P-value:1.78E-7 ID:SGTC_2034|Compound:5197015|FD-Score:2.95|P-value:5.32E-4 ID:SGTC_2041|Compound:5107442|FD-Score:-3.00|P-value:4.58E-4 ID:SGTC_2048|Compound:5246248|FD-Score:7.40|P-value:1.20E-16 ID:SGTC_2057|Compound:5236571|FD-Score:3.44|P-value:6.77E-5 ID:SGTC_2084|Compound:5306078|FD-Score:-2.85|P-value:8.10E-4 ID:SGTC_2121|Compound:5257820|FD-Score:2.82|P-value:8.70E-4 ID:SGTC_2355|Compound:9061122|FD-Score:2.80|P-value:9.52E-4 ID:SGTC_2394|Compound:5221648|FD-Score:3.43|P-value:7.08E-5 ID:SGTC_2395|Compound:5646831|FD-Score:3.89|P-value:7.95E-6 ID:SGTC_2436|Compound:5633522|FD-Score:3.12|P-value:2.65E-4 ID:SGTC_2442|Compound:5158906|FD-Score:2.89|P-value:6.72E-4 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:3.33|P-value:1.12E-4 ID:SGTC_2514|Compound:salinomycin|FD-Score:3.85|P-value:9.99E-6 ID:SGTC_2528|Compound:xanthyletin|FD-Score:3.15|P-value:2.33E-4 ID:SGTC_2529|Compound:gangaleoidin|FD-Score:3.10|P-value:2.97E-4 ID:SGTC_2537|Compound:larixol|FD-Score:4.67|P-value:1.13E-7 ID:SGTC_2588|Compound:indole-3-carbinol|FD-Score:-3.99|P-value:5.20E-6 ID:SGTC_2834|Compound:9001755|FD-Score:-5.38|P-value:1.30E-9 ID:SGTC_2862|Compound:9027634|FD-Score:-2.91|P-value:6.44E-4 ID:SGTC_2876|Compound:9059903|FD-Score:4.18|P-value:1.79E-6 ID:SGTC_3201|Compound:9113999|FD-Score:3.61|P-value:3.15E-5 ID:SGTC_3208|Compound:9114115|FD-Score:-3.54|P-value:4.58E-5 ID:SGTC_3248|Compound:9135562|FD-Score:-3.04|P-value:3.90E-4 ID:SGTC_3254|Compound:9136651|FD-Score:-2.98|P-value:4.98E-4 ID:SGTC_3272|Compound:9137413|FD-Score:-2.80|P-value:9.72E-4 ID:SGTC_3323|Compound:9138564|FD-Score:3.33|P-value:1.08E-4 ID:SGTC_3330|Compound:9141424|FD-Score:-3.31|P-value:1.28E-4

Top fitness defect scores for YGL037C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2048 5246248 7.40 200.0 µM 1.20E-16 1 6.87E-14
2 SGTC_2537 larixol 4.67 68.9 µM 1.13E-7 12 1.51E-6
3 SGTC_2030 5175110 4.59 200.0 µM 1.78E-7 Golgi 17 2.20E-6
4 SGTC_6 0844-0013 4.37 19.8 µM 6.38E-7 57 6.28E-6
5 SGTC_2876 9059903 4.18 26.0 µM 1.79E-6 8 1.46E-5
6 SGTC_2395 5646831 3.89 180.2 µM 7.95E-6 24 4.98E-5
7 SGTC_2514 salinomycin 3.85 42.7 µM 9.99E-6 51 6.01E-5
8 SGTC_3201 9113999 3.61 49.5 µM 3.15E-5 48 1.55E-4
9 SGTC_2057 5236571 3.44 38.3 µM 6.77E-5 RPP1 & pyrimidine depletion 53 2.91E-4
10 SGTC_2394 5221648 3.43 200.0 µM 7.08E-5 41 3.02E-4
11 SGTC_3323 9138564 3.33 53.9 µM 1.08E-4 40 4.27E-4
12 SGTC_2494 18-aminoabieta-8,11,13-triene sulfate 3.33 3.7 µM 1.12E-4 147 4.39E-4
13 SGTC_2528 xanthyletin 3.15 44.8 µM 2.33E-4 46 8.04E-4
14 SGTC_656 4090-1979 3.14 187.0 µM 2.46E-4 51 8.42E-4
15 SGTC_2436 5633522 3.12 8.1 µM 2.65E-4 44 8.94E-4
16 SGTC_2529 gangaleoidin 3.10 93.6 µM 2.97E-4 44 9.83E-4
17 SGTC_915 methyl methanesulfonate (MMS) 3.01 110.0 mM 4.25E-4 DNA damage response 86 0.00132
18 SGTC_1262 0737-0114 2.99 49.6 µM 4.60E-4 64 0.00141
19 SGTC_2034 5197015 2.95 53.1 µM 5.32E-4 SWF1 & branched chain AA biosynthesis 66 0.00159
20 SGTC_781 4335-2806 2.95 94.4 µM 5.40E-4 56 0.00161

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.249 1.98E-48 YKL202W_d YKL202W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.180 1.01E-25 YOR155C ISN1 hom Inosine 5'-monophosphate (IMP)-specific 5'-nucleotidase, catalyzes the breakdown of IMP to inosine, does not show similarity to known 5'-nucleotidases from other organisms
0.161 5.59E-21 YGL017W ATE1 hom Arginyl-tRNA-protein transferase, catalyzes post-translational conjugation of arginine to the amino termini of acceptor proteins which are then subject to degradation via the N-end rule pathway
0.157 5.26E-20 YPL092W SSU1 hom Plasma membrane sulfite pump involved in sulfite metabolism and required for efficient sulfite efflux; major facilitator superfamily protein
0.154 2.50E-19 YDR068W DOS2 hom Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.143 9.34E-17 YLR210W CLB4 hom B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation
0.139 5.06E-16 YFL041W FET5 hom Multicopper oxidase, integral membrane protein with similarity to Fet3p; may have a role in iron transport
0.139 7.41E-16 YNL218W MGS1 hom Protein with DNA-dependent ATPase and ssDNA annealing activities; involved in maintenance of genome; interacts functionally with DNA polymerase delta; homolog of human Werner helicase interacting protein (WHIP); forms nuclear foci upon DNA replication stress
0.138 1.04E-15 YJR019C TES1 hom Peroxisomal acyl-CoA thioesterase likely to be involved in fatty acid oxidation rather than fatty acid synthesis; conserved protein also found in human peroxisomes; TES1 mRNA levels increase during growth on fatty acids
0.136 2.30E-15 YDR505C PSP1 hom Asn and gln rich protein of unknown function; high-copy suppressor of POL1 (DNA polymerase alpha) and partial suppressor of CDC2 (polymerase delta) and CDC6 (pre-RC loading factor) mutations; overexpression results in growth inhibition; PSP1 has a paralog, YLR177W, that arose from the whole genome duplication
0.129 6.39E-14 YLR344W RPL26A hom Ribosomal 60S subunit protein L26A; binds to 5.8S rRNA; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26A has a paralog, RPL26B, that arose from the whole genome duplication
0.128 8.85E-14 YBL086C_p YBL086C_p hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery
0.122 1.13E-12 YDR089W YDR089W hom Protein of unknown function; deletion confers resistance to nickel; contains an SPX domain, which is found in proteins involved in phosphate homeostasis; relocalizes from vacuole to cytoplasm upon DNA replication stress
0.122 1.31E-12 YLR398C SKI2 hom Ski complex component and putative RNA helicase, mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay
0.122 1.52E-12 YMR145C NDE1 hom Mitochondrial external NADH dehydrogenase, a type II NAD(P)H:quinone oxidoreductase that catalyzes the oxidation of cytosolic NADH; Nde1p and Nde2p provide cytosolic NADH to the mitochondrial respiratory chain