YGL044C / RNA15

Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; interacts with the A-rich polyadenylation signal in complex with Rna14p and Hrp1p; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping

Zygosity: Heterozygous strain
fixedexpanded
Profile for YGL044C / RNA15

Click on Significant Values for Screen Details ID:SGTC_13|Compound:0199-0087|FD-Score:3.05|P-value:8.92E-4 ID:SGTC_69|Compound:0096-0274|FD-Score:3.73|P-value:6.79E-5 ID:SGTC_259|Compound:usnic acid|FD-Score:3.75|P-value:6.39E-5 ID:SGTC_276|Compound:colchicine|FD-Score:3.25|P-value:4.47E-4 ID:SGTC_286|Compound:k060-0067|FD-Score:3.69|P-value:7.91E-5 ID:SGTC_354|Compound:2783-3500|FD-Score:3.58|P-value:1.24E-4 ID:SGTC_372|Compound:0155-0182|FD-Score:-6.78|P-value:2.96E-12 ID:SGTC_382|Compound:3229-0966|FD-Score:3.03|P-value:9.75E-4 ID:SGTC_399|Compound:oxolinic acid|FD-Score:-3.48|P-value:2.08E-4 ID:SGTC_430|Compound:0958-0115|FD-Score:4.06|P-value:1.68E-5 ID:SGTC_519|Compound:k048-0011|FD-Score:3.21|P-value:5.05E-4 ID:SGTC_564|Compound:3555-0129|FD-Score:-3.46|P-value:2.27E-4 ID:SGTC_692|Compound:k241-0027|FD-Score:-3.07|P-value:9.48E-4 ID:SGTC_708|Compound:3448-5400|FD-Score:-3.61|P-value:1.29E-4 ID:SGTC_877|Compound:0771-0151|FD-Score:3.07|P-value:8.37E-4 ID:SGTC_966|Compound:1300-0360|FD-Score:-3.06|P-value:9.78E-4 ID:SGTC_1017|Compound:4239-0030|FD-Score:3.51|P-value:1.63E-4 ID:SGTC_1102|Compound:cgs 12066b|FD-Score:3.05|P-value:9.04E-4 ID:SGTC_1144|Compound:3486-0183|FD-Score:3.65|P-value:9.50E-5 ID:SGTC_1167|Compound:k781-0610|FD-Score:3.67|P-value:8.63E-5 ID:SGTC_1185|Compound:1319-0113|FD-Score:3.68|P-value:8.27E-5 ID:SGTC_1197|Compound:1601-0112|FD-Score:4.95|P-value:2.15E-7 ID:SGTC_1229|Compound:0139-0193|FD-Score:6.04|P-value:3.51E-10 ID:SGTC_1233|Compound:0325-0408|FD-Score:4.18|P-value:9.81E-6 ID:SGTC_1238|Compound:0333-0287|FD-Score:3.10|P-value:7.41E-4 ID:SGTC_1244|Compound:0416-0015|FD-Score:5.71|P-value:2.74E-9 ID:SGTC_1247|Compound:0448-0043|FD-Score:4.43|P-value:2.91E-6 ID:SGTC_1286|Compound:0929-0070|FD-Score:3.08|P-value:8.10E-4 ID:SGTC_1530|Compound:rhein|FD-Score:4.14|P-value:1.16E-5 ID:SGTC_1538|Compound:2',3',6-trimethoxyflavanone|FD-Score:4.88|P-value:3.08E-7 ID:SGTC_1598|Compound:3(2'-chlorophenyl)-7-hydroxy-4-phenylcoumarin|FD-Score:-4.42|P-value:3.66E-6 ID:SGTC_1721|Compound:st082152|FD-Score:4.13|P-value:1.22E-5 ID:SGTC_1723|Compound:st034307|FD-Score:-3.97|P-value:2.89E-5 ID:SGTC_1745|Compound:st081495|FD-Score:4.87|P-value:3.23E-7 ID:SGTC_1840|Compound:st055363|FD-Score:4.99|P-value:1.72E-7 ID:SGTC_1878|Compound:temozolomide|FD-Score:3.48|P-value:1.84E-4 ID:SGTC_1889|Compound:st059080|FD-Score:4.02|P-value:1.97E-5 ID:SGTC_1962|Compound:st076595|FD-Score:-3.46|P-value:2.32E-4 ID:SGTC_1964|Compound:st076914|FD-Score:3.24|P-value:4.48E-4 ID:SGTC_1968|Compound:st069590|FD-Score:3.70|P-value:7.71E-5 ID:SGTC_1972|Compound:st070201|FD-Score:5.13|P-value:8.13E-8 ID:SGTC_1976|Compound:st070609|FD-Score:-3.15|P-value:7.21E-4 ID:SGTC_1977|Compound:st070720|FD-Score:-3.36|P-value:3.28E-4 ID:SGTC_2147|Compound:5545932|FD-Score:4.54|P-value:1.71E-6 ID:SGTC_2157|Compound:5649910|FD-Score:3.81|P-value:4.88E-5 ID:SGTC_2163|Compound:5671233|FD-Score:3.53|P-value:1.53E-4 ID:SGTC_2406|Compound:ethylparaben|FD-Score:-4.31|P-value:6.09E-6 ID:SGTC_2443|Compound:5493264|FD-Score:3.73|P-value:6.76E-5 ID:SGTC_2581|Compound:avocatin a|FD-Score:3.05|P-value:9.11E-4 ID:SGTC_2643|Compound:anethole|FD-Score:3.07|P-value:8.24E-4 ID:SGTC_2676|Compound:harmalol|FD-Score:4.19|P-value:9.18E-6 ID:SGTC_2729|Compound:fluphenazine|FD-Score:-3.60|P-value:1.30E-4 ID:SGTC_2794|Compound:7785382|FD-Score:-4.97|P-value:2.35E-7 ID:SGTC_2801|Compound:7907800|FD-Score:6.49|P-value:1.77E-11 ID:SGTC_2873|Compound:9040596|FD-Score:3.03|P-value:9.60E-4 ID:SGTC_2987|Compound:9018338|FD-Score:4.81|P-value:4.40E-7 ID:SGTC_3136|Compound:9095672|FD-Score:3.33|P-value:3.28E-4 ID:SGTC_3219|Compound:9131112|FD-Score:3.68|P-value:8.23E-5 ID:SGTC_3282|Compound:9108498|FD-Score:3.61|P-value:1.10E-4 ID:SGTC_3285|Compound:9112818|FD-Score:3.65|P-value:9.41E-5 ID:SGTC_13|Compound:0199-0087|FD-Score:3.05|P-value:8.92E-4 ID:SGTC_69|Compound:0096-0274|FD-Score:3.73|P-value:6.79E-5 ID:SGTC_259|Compound:usnic acid|FD-Score:3.75|P-value:6.39E-5 ID:SGTC_276|Compound:colchicine|FD-Score:3.25|P-value:4.47E-4 ID:SGTC_286|Compound:k060-0067|FD-Score:3.69|P-value:7.91E-5 ID:SGTC_354|Compound:2783-3500|FD-Score:3.58|P-value:1.24E-4 ID:SGTC_372|Compound:0155-0182|FD-Score:-6.78|P-value:2.96E-12 ID:SGTC_382|Compound:3229-0966|FD-Score:3.03|P-value:9.75E-4 ID:SGTC_399|Compound:oxolinic acid|FD-Score:-3.48|P-value:2.08E-4 ID:SGTC_430|Compound:0958-0115|FD-Score:4.06|P-value:1.68E-5 ID:SGTC_519|Compound:k048-0011|FD-Score:3.21|P-value:5.05E-4 ID:SGTC_564|Compound:3555-0129|FD-Score:-3.46|P-value:2.27E-4 ID:SGTC_692|Compound:k241-0027|FD-Score:-3.07|P-value:9.48E-4 ID:SGTC_708|Compound:3448-5400|FD-Score:-3.61|P-value:1.29E-4 ID:SGTC_877|Compound:0771-0151|FD-Score:3.07|P-value:8.37E-4 ID:SGTC_966|Compound:1300-0360|FD-Score:-3.06|P-value:9.78E-4 ID:SGTC_1017|Compound:4239-0030|FD-Score:3.51|P-value:1.63E-4 ID:SGTC_1102|Compound:cgs 12066b|FD-Score:3.05|P-value:9.04E-4 ID:SGTC_1144|Compound:3486-0183|FD-Score:3.65|P-value:9.50E-5 ID:SGTC_1167|Compound:k781-0610|FD-Score:3.67|P-value:8.63E-5 ID:SGTC_1185|Compound:1319-0113|FD-Score:3.68|P-value:8.27E-5 ID:SGTC_1197|Compound:1601-0112|FD-Score:4.95|P-value:2.15E-7 ID:SGTC_1229|Compound:0139-0193|FD-Score:6.04|P-value:3.51E-10 ID:SGTC_1233|Compound:0325-0408|FD-Score:4.18|P-value:9.81E-6 ID:SGTC_1238|Compound:0333-0287|FD-Score:3.10|P-value:7.41E-4 ID:SGTC_1244|Compound:0416-0015|FD-Score:5.71|P-value:2.74E-9 ID:SGTC_1247|Compound:0448-0043|FD-Score:4.43|P-value:2.91E-6 ID:SGTC_1286|Compound:0929-0070|FD-Score:3.08|P-value:8.10E-4 ID:SGTC_1530|Compound:rhein|FD-Score:4.14|P-value:1.16E-5 ID:SGTC_1538|Compound:2',3',6-trimethoxyflavanone|FD-Score:4.88|P-value:3.08E-7 ID:SGTC_1598|Compound:3(2'-chlorophenyl)-7-hydroxy-4-phenylcoumarin|FD-Score:-4.42|P-value:3.66E-6 ID:SGTC_1721|Compound:st082152|FD-Score:4.13|P-value:1.22E-5 ID:SGTC_1723|Compound:st034307|FD-Score:-3.97|P-value:2.89E-5 ID:SGTC_1745|Compound:st081495|FD-Score:4.87|P-value:3.23E-7 ID:SGTC_1840|Compound:st055363|FD-Score:4.99|P-value:1.72E-7 ID:SGTC_1878|Compound:temozolomide|FD-Score:3.48|P-value:1.84E-4 ID:SGTC_1889|Compound:st059080|FD-Score:4.02|P-value:1.97E-5 ID:SGTC_1962|Compound:st076595|FD-Score:-3.46|P-value:2.32E-4 ID:SGTC_1964|Compound:st076914|FD-Score:3.24|P-value:4.48E-4 ID:SGTC_1968|Compound:st069590|FD-Score:3.70|P-value:7.71E-5 ID:SGTC_1972|Compound:st070201|FD-Score:5.13|P-value:8.13E-8 ID:SGTC_1976|Compound:st070609|FD-Score:-3.15|P-value:7.21E-4 ID:SGTC_1977|Compound:st070720|FD-Score:-3.36|P-value:3.28E-4 ID:SGTC_2147|Compound:5545932|FD-Score:4.54|P-value:1.71E-6 ID:SGTC_2157|Compound:5649910|FD-Score:3.81|P-value:4.88E-5 ID:SGTC_2163|Compound:5671233|FD-Score:3.53|P-value:1.53E-4 ID:SGTC_2406|Compound:ethylparaben|FD-Score:-4.31|P-value:6.09E-6 ID:SGTC_2443|Compound:5493264|FD-Score:3.73|P-value:6.76E-5 ID:SGTC_2581|Compound:avocatin a|FD-Score:3.05|P-value:9.11E-4 ID:SGTC_2643|Compound:anethole|FD-Score:3.07|P-value:8.24E-4 ID:SGTC_2676|Compound:harmalol|FD-Score:4.19|P-value:9.18E-6 ID:SGTC_2729|Compound:fluphenazine|FD-Score:-3.60|P-value:1.30E-4 ID:SGTC_2794|Compound:7785382|FD-Score:-4.97|P-value:2.35E-7 ID:SGTC_2801|Compound:7907800|FD-Score:6.49|P-value:1.77E-11 ID:SGTC_2873|Compound:9040596|FD-Score:3.03|P-value:9.60E-4 ID:SGTC_2987|Compound:9018338|FD-Score:4.81|P-value:4.40E-7 ID:SGTC_3136|Compound:9095672|FD-Score:3.33|P-value:3.28E-4 ID:SGTC_3219|Compound:9131112|FD-Score:3.68|P-value:8.23E-5 ID:SGTC_3282|Compound:9108498|FD-Score:3.61|P-value:1.10E-4 ID:SGTC_3285|Compound:9112818|FD-Score:3.65|P-value:9.41E-5

Top fitness defect scores for YGL044C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2801 7907800 6.49 81.8 µM 1.77E-11 2 4.36E-11 0.98
2 SGTC_1229 0139-0193 6.04 12.2 µM 3.51E-10 RSC complex & mRNA processing 8 7.75E-10 0.09
3 SGTC_1244 0416-0015 5.71 52.5 µM 2.74E-9 ERG2 1 5.62E-9 1.12
4 SGTC_1972 st070201 5.13 17.4 µM 8.13E-8 1 1.47E-7 0.83
5 SGTC_1840 st055363 4.99 71.0 µM 1.72E-7 2 3.02E-7 1.24
6 SGTC_1197 1601-0112 4.95 267.0 µM 2.15E-7 3 3.75E-7 0.51
7 SGTC_1538 2',3',6-trimethoxyflavanone 4.88 63.6 µM 3.08E-7 1 5.30E-7 1.10
8 SGTC_1745 st081495 4.87 74.0 µM 3.23E-7 RNA processing & uracil transport 19 5.55E-7 0.04
9 SGTC_2987 9018338 4.81 71.4 µM 4.40E-7 RPP1 & pyrimidine depletion 4 7.46E-7 0.51
10 SGTC_2147 5545932 4.54 89.7 µM 1.71E-6 ergosterol biosynthesis 8 2.75E-6 0.18
11 SGTC_1247 0448-0043 4.43 5.8 µM 2.91E-6 ERG2 7 4.61E-6 0.11
12 SGTC_2676 harmalol 4.19 100.0 µM 9.18E-6 mitochondrial processes 4 1.39E-5 0.04
13 SGTC_1233 0325-0408 4.18 14.2 µM 9.81E-6 4 1.48E-5 0.19
14 SGTC_1530 rhein 4.14 70.4 µM 1.16E-5 1 1.74E-5 0.38
15 SGTC_1721 st082152 4.13 9.3 µM 1.22E-5 60S ribosome export 8 1.83E-5 0.07
16 SGTC_430 0958-0115 4.06 16.4 µM 1.68E-5 8 2.49E-5 0.01
17 SGTC_1889 st059080 4.02 5.2 µM 1.97E-5 4 2.90E-5 0.16
18 SGTC_2157 5649910 3.81 96.4 µM 4.88E-5 7 6.95E-5 0.00
19 SGTC_259 usnic acid 3.75 108.9 µM 6.39E-5 8 9.00E-5 0.02
20 SGTC_2443 5493264 3.73 126.7 µM 6.76E-5 9 9.51E-5 0.14

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.125 4.44E-13 YGR245C SDA1 het Highly conserved nuclear protein required for actin cytoskeleton organization and passage through Start, plays a critical role in G1 events, binds Nap1p, also involved in 60S ribosome biogenesis
0.119 4.70E-12 YJL217W REE1 hom Cytoplasmic protein involved in the regulation of enolase (ENO1); mRNA expression is induced by calcium shortage, copper deficiency (via Mac1p) and the presence of galactose (via Gal4p); mRNA expression is also regulated by the cell cycle
0.118 5.73E-12 YHR164C DNA2 het Tripartite DNA replication factor; has single-stranded DNA-dependent ATPase, ATP-dependent nuclease, and helicase activities; required for Okazaki fragment processing; involved in DNA repair; cell-cycle dependent localization; forms nuclear foci upon DNA replication stress
0.107 5.59E-10 YPR178W PRP4 het Splicing factor, component of the U4/U6-U5 snRNP complex
0.104 1.41E-9 YOL005C RPB11 het RNA polymerase II subunit B12.5; part of central core; similar to Rpc19p and bacterial alpha subunit
0.090 1.95E-7 YOL026C MIM1 het Mitochondrial protein required for outer membrane protein import; cooperates with Tom70p to import the subset of proteins with multiple alpha-helical transmembrane segments, including Ugo1p, Tom20p, and others; present in a complex with Mim2p in the outer membrane that may create a local environment to facilitate membrane insertion of substrate proteins; also has a role in assembly of Tom20p into the TOM complex
0.088 3.78E-7 YMR061W RNA14 het Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; bridges interaction between Rna15p and Hrp1p in the CF I complex; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping
0.087 4.44E-7 YKR031C SPO14 hom Phospholipase D; catalyzes the hydrolysis of phosphatidylcholine, producing choline and phosphatidic acid; involved in Sec14p-independent secretion; required for meiosis and spore formation; differently regulated in secretion and meiosis; participates in transcription initiation and/or early elongation of specific genes; interacts with foot domain of RNA polymerase II; deletion results in abnormal CTD-Ser5 phosphorylation of RNA polymerase II at specific promoter regions
0.086 5.79E-7 YLL011W SOF1 het Essential protein required for biogenesis of 40S (small) ribosomal subunit; has similarity to the beta subunit of trimeric G-proteins and the splicing factor Prp4p
0.084 1.04E-6 YLR183C TOS4 hom Putative transcription factor, contains Forkhead Associated domain; found associated with chromatin; target of SBF transcription factor; expression is periodic and peaks in G1; relative distribution to the nucleus increases upon DNA replication stress; TOS4 has a paralog, PLM2, that arose from the whole genome duplication
0.084 1.10E-6 YDR020C_p DAS2_p hom Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; weak similarity with uridine kinases and with phosphoribokinases
0.084 1.21E-6 YDR290W_d YDR290W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RTT103
0.084 1.26E-6 YJR133W XPT1 hom Xanthine-guanine phosphoribosyl transferase, required for xanthine utilization and for optimal utilization of guanine
0.083 1.41E-6 YDL141W BPL1 het Biotin:apoprotein ligase, covalently modifies proteins with the addition of biotin, required for acetyl-CoA carboxylase (Acc1p) holoenzyme formation
0.083 1.61E-6 YHL041W_d YHL041W_d hom Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data