YGL048C / RPT6

ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle; protein abundance increases in response to DNA replication stress

Zygosity: Heterozygous strain
fixedexpanded
Profile for YGL048C / RPT6

Click on Significant Values for Screen Details ID:SGTC_127|Compound:0180-0333|FD-Score:-5.52|P-value:5.37E-8 ID:SGTC_128|Compound:0906-2981|FD-Score:5.04|P-value:4.53E-7 ID:SGTC_131|Compound:0141-0277|FD-Score:4.70|P-value:2.23E-6 ID:SGTC_138|Compound:0850-0259|FD-Score:-4.79|P-value:2.01E-6 ID:SGTC_141|Compound:0443-0274|FD-Score:-7.20|P-value:1.93E-12 ID:SGTC_236|Compound:1509-0011|FD-Score:6.10|P-value:1.44E-9 ID:SGTC_332|Compound:4112-0506|FD-Score:4.15|P-value:2.57E-5 ID:SGTC_339|Compound:3091-4833|FD-Score:4.15|P-value:2.57E-5 ID:SGTC_431|Compound:k089-0097|FD-Score:3.17|P-value:9.68E-4 ID:SGTC_444|Compound:curcumin|FD-Score:3.76|P-value:1.19E-4 ID:SGTC_497|Compound:capsazepine|FD-Score:3.18|P-value:9.14E-4 ID:SGTC_528|Compound:3492-0019|FD-Score:-4.21|P-value:2.60E-5 ID:SGTC_546|Compound:peri-Naphthalide|FD-Score:4.37|P-value:9.93E-6 ID:SGTC_547|Compound:1120-0019|FD-Score:-4.38|P-value:1.27E-5 ID:SGTC_553|Compound:k007-0256|FD-Score:4.39|P-value:9.31E-6 ID:SGTC_584|Compound:0986-0246|FD-Score:5.37|P-value:8.12E-8 ID:SGTC_634|Compound:1611-4317|FD-Score:3.85|P-value:8.56E-5 ID:SGTC_772|Compound:k072-0172|FD-Score:3.63|P-value:1.93E-4 ID:SGTC_837|Compound:0091-0265|FD-Score:-3.69|P-value:1.97E-4 ID:SGTC_897|Compound:1000-0139|FD-Score:3.24|P-value:7.73E-4 ID:SGTC_1016|Compound:4226-1401|FD-Score:4.30|P-value:1.36E-5 ID:SGTC_1044|Compound:1222-0021|FD-Score:4.78|P-value:1.55E-6 ID:SGTC_1098|Compound:clofazimine|FD-Score:4.62|P-value:3.27E-6 ID:SGTC_1113|Compound:artemisinin|FD-Score:3.60|P-value:2.13E-4 ID:SGTC_1124|Compound:fisetin|FD-Score:4.87|P-value:1.03E-6 ID:SGTC_1168|Compound:k815-0004|FD-Score:3.60|P-value:2.16E-4 ID:SGTC_1171|Compound:0368-0077|FD-Score:3.20|P-value:8.71E-4 ID:SGTC_1184|Compound:1269-1718|FD-Score:-3.28|P-value:8.33E-4 ID:SGTC_1188|Compound:1403-0014|FD-Score:3.57|P-value:2.41E-4 ID:SGTC_1226|Compound:3909-7979|FD-Score:4.31|P-value:1.28E-5 ID:SGTC_1255|Compound:0566-0045|FD-Score:3.57|P-value:2.43E-4 ID:SGTC_1347|Compound:1493-0251|FD-Score:-4.38|P-value:1.28E-5 ID:SGTC_1552|Compound:6-methylflavone|FD-Score:5.74|P-value:1.13E-8 ID:SGTC_1556|Compound:plumbagin|FD-Score:3.54|P-value:2.72E-4 ID:SGTC_1564|Compound:D-Arginine|FD-Score:3.38|P-value:4.73E-4 ID:SGTC_1595|Compound:6-ethoxy-3(4'-hydroxyphenyl)-4-methylcoumarin|FD-Score:3.23|P-value:7.85E-4 ID:SGTC_1641|Compound:st009299|FD-Score:3.22|P-value:8.11E-4 ID:SGTC_1667|Compound:st014849|FD-Score:-3.56|P-value:3.19E-4 ID:SGTC_1787|Compound:fluconazole|FD-Score:-3.67|P-value:2.18E-4 ID:SGTC_1917|Compound:st066507|FD-Score:3.17|P-value:9.60E-4 ID:SGTC_2004|Compound:4023728|FD-Score:4.71|P-value:2.16E-6 ID:SGTC_2071|Compound:5230951|FD-Score:4.85|P-value:1.12E-6 ID:SGTC_2073|Compound:5212005|FD-Score:3.44|P-value:3.83E-4 ID:SGTC_2075|Compound:5212009|FD-Score:5.39|P-value:7.33E-8 ID:SGTC_2081|Compound:5214970|FD-Score:-4.33|P-value:1.62E-5 ID:SGTC_2124|Compound:5246320|FD-Score:6.33|P-value:3.58E-10 ID:SGTC_2169|Compound:5-(ethoxymethyl)quinolin-8-ol|FD-Score:4.89|P-value:9.15E-7 ID:SGTC_2225|Compound:6654152|FD-Score:4.00|P-value:4.74E-5 ID:SGTC_2256|Compound:7954252|FD-Score:4.32|P-value:1.25E-5 ID:SGTC_2289|Compound:7946404|FD-Score:5.63|P-value:2.09E-8 ID:SGTC_2322|Compound:7413319|FD-Score:-3.87|P-value:1.02E-4 ID:SGTC_2329|Compound:6138490|FD-Score:4.85|P-value:1.14E-6 ID:SGTC_2330|Compound:7979373|FD-Score:-3.27|P-value:8.54E-4 ID:SGTC_2336|Compound:9001819|FD-Score:6.48|P-value:1.39E-10 ID:SGTC_2344|Compound:7990960|FD-Score:3.29|P-value:6.53E-4 ID:SGTC_2348|Compound:9022543|FD-Score:3.47|P-value:3.43E-4 ID:SGTC_2402|Compound:5553605|FD-Score:3.20|P-value:8.81E-4 ID:SGTC_2451|Compound:5856223|FD-Score:4.39|P-value:9.15E-6 ID:SGTC_2519|Compound:cryptotanshinone|FD-Score:4.28|P-value:1.45E-5 ID:SGTC_2707|Compound:st078556|FD-Score:3.33|P-value:5.58E-4 ID:SGTC_2825|Compound:7998094|FD-Score:3.30|P-value:6.30E-4 ID:SGTC_2896|Compound:9052656|FD-Score:-3.79|P-value:1.39E-4 ID:SGTC_2965|Compound:9082772|FD-Score:-4.08|P-value:4.52E-5 ID:SGTC_2996|Compound:9066192|FD-Score:3.18|P-value:9.40E-4 ID:SGTC_3062|Compound:9077494|FD-Score:-4.02|P-value:5.75E-5 ID:SGTC_3127|Compound:9126946|FD-Score:5.10|P-value:3.29E-7 ID:SGTC_3142|Compound:9086498|FD-Score:7.56|P-value:9.30E-14 ID:SGTC_3193|Compound:9111931|FD-Score:-3.36|P-value:6.33E-4 ID:SGTC_3304|Compound:9125678|FD-Score:-3.24|P-value:9.54E-4 ID:SGTC_3317|Compound:9138495|FD-Score:5.18|P-value:2.24E-7 ID:SGTC_3318|Compound:9139014|FD-Score:6.18|P-value:8.77E-10 ID:SGTC_3327|Compound:9140648|FD-Score:5.24|P-value:1.61E-7 ID:SGTC_127|Compound:0180-0333|FD-Score:-5.52|P-value:5.37E-8 ID:SGTC_128|Compound:0906-2981|FD-Score:5.04|P-value:4.53E-7 ID:SGTC_131|Compound:0141-0277|FD-Score:4.70|P-value:2.23E-6 ID:SGTC_138|Compound:0850-0259|FD-Score:-4.79|P-value:2.01E-6 ID:SGTC_141|Compound:0443-0274|FD-Score:-7.20|P-value:1.93E-12 ID:SGTC_236|Compound:1509-0011|FD-Score:6.10|P-value:1.44E-9 ID:SGTC_332|Compound:4112-0506|FD-Score:4.15|P-value:2.57E-5 ID:SGTC_339|Compound:3091-4833|FD-Score:4.15|P-value:2.57E-5 ID:SGTC_431|Compound:k089-0097|FD-Score:3.17|P-value:9.68E-4 ID:SGTC_444|Compound:curcumin|FD-Score:3.76|P-value:1.19E-4 ID:SGTC_497|Compound:capsazepine|FD-Score:3.18|P-value:9.14E-4 ID:SGTC_528|Compound:3492-0019|FD-Score:-4.21|P-value:2.60E-5 ID:SGTC_546|Compound:peri-Naphthalide|FD-Score:4.37|P-value:9.93E-6 ID:SGTC_547|Compound:1120-0019|FD-Score:-4.38|P-value:1.27E-5 ID:SGTC_553|Compound:k007-0256|FD-Score:4.39|P-value:9.31E-6 ID:SGTC_584|Compound:0986-0246|FD-Score:5.37|P-value:8.12E-8 ID:SGTC_634|Compound:1611-4317|FD-Score:3.85|P-value:8.56E-5 ID:SGTC_772|Compound:k072-0172|FD-Score:3.63|P-value:1.93E-4 ID:SGTC_837|Compound:0091-0265|FD-Score:-3.69|P-value:1.97E-4 ID:SGTC_897|Compound:1000-0139|FD-Score:3.24|P-value:7.73E-4 ID:SGTC_1016|Compound:4226-1401|FD-Score:4.30|P-value:1.36E-5 ID:SGTC_1044|Compound:1222-0021|FD-Score:4.78|P-value:1.55E-6 ID:SGTC_1098|Compound:clofazimine|FD-Score:4.62|P-value:3.27E-6 ID:SGTC_1113|Compound:artemisinin|FD-Score:3.60|P-value:2.13E-4 ID:SGTC_1124|Compound:fisetin|FD-Score:4.87|P-value:1.03E-6 ID:SGTC_1168|Compound:k815-0004|FD-Score:3.60|P-value:2.16E-4 ID:SGTC_1171|Compound:0368-0077|FD-Score:3.20|P-value:8.71E-4 ID:SGTC_1184|Compound:1269-1718|FD-Score:-3.28|P-value:8.33E-4 ID:SGTC_1188|Compound:1403-0014|FD-Score:3.57|P-value:2.41E-4 ID:SGTC_1226|Compound:3909-7979|FD-Score:4.31|P-value:1.28E-5 ID:SGTC_1255|Compound:0566-0045|FD-Score:3.57|P-value:2.43E-4 ID:SGTC_1347|Compound:1493-0251|FD-Score:-4.38|P-value:1.28E-5 ID:SGTC_1552|Compound:6-methylflavone|FD-Score:5.74|P-value:1.13E-8 ID:SGTC_1556|Compound:plumbagin|FD-Score:3.54|P-value:2.72E-4 ID:SGTC_1564|Compound:D-Arginine|FD-Score:3.38|P-value:4.73E-4 ID:SGTC_1595|Compound:6-ethoxy-3(4'-hydroxyphenyl)-4-methylcoumarin|FD-Score:3.23|P-value:7.85E-4 ID:SGTC_1641|Compound:st009299|FD-Score:3.22|P-value:8.11E-4 ID:SGTC_1667|Compound:st014849|FD-Score:-3.56|P-value:3.19E-4 ID:SGTC_1787|Compound:fluconazole|FD-Score:-3.67|P-value:2.18E-4 ID:SGTC_1917|Compound:st066507|FD-Score:3.17|P-value:9.60E-4 ID:SGTC_2004|Compound:4023728|FD-Score:4.71|P-value:2.16E-6 ID:SGTC_2071|Compound:5230951|FD-Score:4.85|P-value:1.12E-6 ID:SGTC_2073|Compound:5212005|FD-Score:3.44|P-value:3.83E-4 ID:SGTC_2075|Compound:5212009|FD-Score:5.39|P-value:7.33E-8 ID:SGTC_2081|Compound:5214970|FD-Score:-4.33|P-value:1.62E-5 ID:SGTC_2124|Compound:5246320|FD-Score:6.33|P-value:3.58E-10 ID:SGTC_2169|Compound:5-(ethoxymethyl)quinolin-8-ol|FD-Score:4.89|P-value:9.15E-7 ID:SGTC_2225|Compound:6654152|FD-Score:4.00|P-value:4.74E-5 ID:SGTC_2256|Compound:7954252|FD-Score:4.32|P-value:1.25E-5 ID:SGTC_2289|Compound:7946404|FD-Score:5.63|P-value:2.09E-8 ID:SGTC_2322|Compound:7413319|FD-Score:-3.87|P-value:1.02E-4 ID:SGTC_2329|Compound:6138490|FD-Score:4.85|P-value:1.14E-6 ID:SGTC_2330|Compound:7979373|FD-Score:-3.27|P-value:8.54E-4 ID:SGTC_2336|Compound:9001819|FD-Score:6.48|P-value:1.39E-10 ID:SGTC_2344|Compound:7990960|FD-Score:3.29|P-value:6.53E-4 ID:SGTC_2348|Compound:9022543|FD-Score:3.47|P-value:3.43E-4 ID:SGTC_2402|Compound:5553605|FD-Score:3.20|P-value:8.81E-4 ID:SGTC_2451|Compound:5856223|FD-Score:4.39|P-value:9.15E-6 ID:SGTC_2519|Compound:cryptotanshinone|FD-Score:4.28|P-value:1.45E-5 ID:SGTC_2707|Compound:st078556|FD-Score:3.33|P-value:5.58E-4 ID:SGTC_2825|Compound:7998094|FD-Score:3.30|P-value:6.30E-4 ID:SGTC_2896|Compound:9052656|FD-Score:-3.79|P-value:1.39E-4 ID:SGTC_2965|Compound:9082772|FD-Score:-4.08|P-value:4.52E-5 ID:SGTC_2996|Compound:9066192|FD-Score:3.18|P-value:9.40E-4 ID:SGTC_3062|Compound:9077494|FD-Score:-4.02|P-value:5.75E-5 ID:SGTC_3127|Compound:9126946|FD-Score:5.10|P-value:3.29E-7 ID:SGTC_3142|Compound:9086498|FD-Score:7.56|P-value:9.30E-14 ID:SGTC_3193|Compound:9111931|FD-Score:-3.36|P-value:6.33E-4 ID:SGTC_3304|Compound:9125678|FD-Score:-3.24|P-value:9.54E-4 ID:SGTC_3317|Compound:9138495|FD-Score:5.18|P-value:2.24E-7 ID:SGTC_3318|Compound:9139014|FD-Score:6.18|P-value:8.77E-10 ID:SGTC_3327|Compound:9140648|FD-Score:5.24|P-value:1.61E-7

Top fitness defect scores for YGL048C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_3142 9086498 7.56 49.5 µM 9.30E-14 iron homeostasis 16 1.95E-14 0.12
2 SGTC_2336 9001819 6.48 198.7 µM 1.39E-10 heme biosynthesis & mitochondrial translocase 21 4.53E-11 0.60
3 SGTC_2124 5246320 6.33 200.0 µM 3.58E-10 heme biosynthesis & mitochondrial translocase 13 1.23E-10 0.30
4 SGTC_3318 9139014 6.18 38.6 µM 8.77E-10 2 3.18E-10 0.94
5 SGTC_236 1509-0011 6.10 5.3 µM 1.44E-9 9 5.38E-10 0.39
6 SGTC_1552 6-methylflavone 5.74 10.6 µM 1.13E-8 superoxide 7 4.76E-9 0.97
7 SGTC_2289 7946404 5.63 165.1 µM 2.09E-8 7 9.12E-9 0.07
8 SGTC_2075 5212009 5.39 200.0 µM 7.33E-8 1 3.44E-8 0.41
9 SGTC_584 0986-0246 5.37 15.4 µM 8.12E-8 heme requiring 10 3.84E-8 0.11
10 SGTC_3327 9140648 5.24 26.9 µM 1.61E-7 5 7.91E-8 0.06
11 SGTC_3317 9138495 5.18 8.8 µM 2.24E-7 8 1.12E-7 1.09
12 SGTC_3127 9126946 5.10 49.5 µM 3.29E-7 ERG2 7 1.69E-7 0.48
13 SGTC_128 0906-2981 5.04 13.4 µM 4.53E-7 3 2.36E-7 0.38
14 SGTC_2169 5-(ethoxymethyl)quinolin-8-ol 4.89 49.5 µM 9.15E-7 11 4.97E-7 0.12
15 SGTC_1124 fisetin 4.87 1.2 µM 1.03E-6 6 5.62E-7 0.11
16 SGTC_2071 5230951 4.85 39.9 µM 1.12E-6 TSC3-RPN4 6 6.13E-7 0.94
17 SGTC_2329 6138490 4.85 200.0 µM 1.14E-6 6 6.29E-7 0.39
18 SGTC_1044 1222-0021 4.78 1.8 µM 1.55E-6 heme biosynthesis & mitochondrial translocase 26 8.68E-7 0.04
19 SGTC_2004 4023728 4.71 41.9 µM 2.16E-6 18 1.23E-6 0.01
20 SGTC_131 0141-0277 4.70 33.9 µM 2.23E-6 5 1.27E-6 0.12

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.383 2.68E-117 YOR117W RPT5 het One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1
0.380 1.62E-115 YER021W RPN3 het Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control
0.318 6.38E-80 YDL147W RPN5 het Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein
0.287 1.65E-64 YDR427W RPN9 het Non-ATPase regulatory subunit of the 26S proteasome; has similarity to putative proteasomal subunits in other species; null mutant is temperature sensitive and exhibits cell cycle and proteasome assembly defects; protein abundance increases in response to DNA replication stress
0.270 5.12E-57 YDR394W RPT3 het One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B
0.253 6.29E-50 YDL097C RPN6 het Essential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress
0.246 1.30E-47 YDL007W RPT2 het One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for normal peptide hydrolysis by the core 20S particle
0.229 3.84E-41 YDL205C HEM3 het Porphobilinogen deaminase, catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in heme biosynthesis; localizes to the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3p, but not by levels of heme
0.224 2.55E-39 YFR052W RPN12 het Subunit of the 19S regulatory particle of the 26S proteasome lid; synthetically lethal with RPT1, which is an ATPase component of the 19S regulatory particle; physically interacts with Nob1p and Rpn3p; protein abundance increases in response to DNA replication stress
0.219 1.11E-37 YFR004W RPN11 het Metalloprotease subunit of the 19S regulatory particle of the 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress
0.190 1.06E-28 YBL105C PKC1 het Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC)
0.187 9.27E-28 YBR192W RIM2 het Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family
0.178 2.88E-25 YOR261C RPN8 het Essential, non-ATPase regulatory subunit of the 26S proteasome; has similarity to the human p40 proteasomal subunit and to another S. cerevisiae regulatory subunit, Rpn11p
0.175 1.61E-24 YKL195W MIA40 het Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p
0.173 4.32E-24 YOR259C RPT4 het One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in degradation of ubiquitinated substrates; contributes preferentially to ERAD; required for spindle pole body duplication; mainly nuclear localization