YGL049C / TIF4632

Translation initiation factor eIF4G, subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); associates with the poly(A)-binding protein Pab1p, also interacts with eIF4A (Tif1p); TIF4632 has a paralog, TIF4631, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YGL049C / TIF4632

Click on Significant Values for Screen Details ID:SGTC_59|Compound:0330-0160|FD-Score:2.92|P-value:5.82E-4 ID:SGTC_65|Compound:0798-0905|FD-Score:-3.05|P-value:3.46E-4 ID:SGTC_87|Compound:0100-0052|FD-Score:3.32|P-value:1.13E-4 ID:SGTC_108|Compound:0509-0257|FD-Score:2.94|P-value:5.35E-4 ID:SGTC_115|Compound:0527-0192|FD-Score:4.03|P-value:3.62E-6 ID:SGTC_414|Compound:piperonyl butoxide|FD-Score:3.15|P-value:2.28E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:3.24|P-value:1.58E-4 ID:SGTC_532|Compound:1548-0774|FD-Score:4.07|P-value:2.93E-6 ID:SGTC_568|Compound:1193-0097|FD-Score:2.92|P-value:5.83E-4 ID:SGTC_1050|Compound:3232-1156|FD-Score:3.93|P-value:6.07E-6 ID:SGTC_1100|Compound:amiodarone|FD-Score:3.22|P-value:1.73E-4 ID:SGTC_1105|Compound:epirubicin|FD-Score:4.56|P-value:1.96E-7 ID:SGTC_1205|Compound:4161-2785|FD-Score:2.90|P-value:6.28E-4 ID:SGTC_1259|Compound:0671-0132|FD-Score:-3.26|P-value:1.41E-4 ID:SGTC_1364|Compound:1653-0028|FD-Score:2.89|P-value:6.45E-4 ID:SGTC_1439|Compound:4092-1168|FD-Score:2.88|P-value:6.90E-4 ID:SGTC_1521|Compound:st012942|FD-Score:-2.97|P-value:4.76E-4 ID:SGTC_1618|Compound:st003135|FD-Score:4.01|P-value:4.11E-6 ID:SGTC_1676|Compound:st018489|FD-Score:-3.13|P-value:2.49E-4 ID:SGTC_2068|Compound:5226020|FD-Score:-3.38|P-value:8.66E-5 ID:SGTC_2126|Compound:5251174|FD-Score:2.81|P-value:8.77E-4 ID:SGTC_2272|Compound:7805186|FD-Score:3.65|P-value:2.47E-5 ID:SGTC_2304|Compound:7443016|FD-Score:3.22|P-value:1.69E-4 ID:SGTC_2305|Compound:7443522|FD-Score:2.96|P-value:4.98E-4 ID:SGTC_2325|Compound:6158266|FD-Score:-3.65|P-value:2.44E-5 ID:SGTC_2449|Compound:5812982|FD-Score:2.93|P-value:5.52E-4 ID:SGTC_2740|Compound:felodipine|FD-Score:2.94|P-value:5.28E-4 ID:SGTC_2755|Compound:lomerizine|FD-Score:-3.99|P-value:4.62E-6 ID:SGTC_2854|Compound:9024559|FD-Score:3.41|P-value:7.54E-5 ID:SGTC_2855|Compound:9016525|FD-Score:3.07|P-value:3.22E-4 ID:SGTC_2858|Compound:9025781|FD-Score:3.76|P-value:1.47E-5 ID:SGTC_2893|Compound:9048488|FD-Score:-3.01|P-value:4.03E-4 ID:SGTC_3205|Compound:9113483|FD-Score:3.70|P-value:1.94E-5 ID:SGTC_3212|Compound:9128307|FD-Score:-2.78|P-value:9.73E-4 ID:SGTC_3264|Compound:9137442|FD-Score:2.85|P-value:7.61E-4 ID:SGTC_3265|Compound:9137266|FD-Score:2.87|P-value:7.19E-4 ID:SGTC_3266|Compound:9136860|FD-Score:3.16|P-value:2.22E-4 ID:SGTC_3277|Compound:9138724|FD-Score:3.35|P-value:9.85E-5 ID:SGTC_3304|Compound:9125678|FD-Score:5.46|P-value:6.37E-10 ID:SGTC_3311|Compound:9131146|FD-Score:-2.85|P-value:7.49E-4 ID:SGTC_59|Compound:0330-0160|FD-Score:2.92|P-value:5.82E-4 ID:SGTC_65|Compound:0798-0905|FD-Score:-3.05|P-value:3.46E-4 ID:SGTC_87|Compound:0100-0052|FD-Score:3.32|P-value:1.13E-4 ID:SGTC_108|Compound:0509-0257|FD-Score:2.94|P-value:5.35E-4 ID:SGTC_115|Compound:0527-0192|FD-Score:4.03|P-value:3.62E-6 ID:SGTC_414|Compound:piperonyl butoxide|FD-Score:3.15|P-value:2.28E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:3.24|P-value:1.58E-4 ID:SGTC_532|Compound:1548-0774|FD-Score:4.07|P-value:2.93E-6 ID:SGTC_568|Compound:1193-0097|FD-Score:2.92|P-value:5.83E-4 ID:SGTC_1050|Compound:3232-1156|FD-Score:3.93|P-value:6.07E-6 ID:SGTC_1100|Compound:amiodarone|FD-Score:3.22|P-value:1.73E-4 ID:SGTC_1105|Compound:epirubicin|FD-Score:4.56|P-value:1.96E-7 ID:SGTC_1205|Compound:4161-2785|FD-Score:2.90|P-value:6.28E-4 ID:SGTC_1259|Compound:0671-0132|FD-Score:-3.26|P-value:1.41E-4 ID:SGTC_1364|Compound:1653-0028|FD-Score:2.89|P-value:6.45E-4 ID:SGTC_1439|Compound:4092-1168|FD-Score:2.88|P-value:6.90E-4 ID:SGTC_1521|Compound:st012942|FD-Score:-2.97|P-value:4.76E-4 ID:SGTC_1618|Compound:st003135|FD-Score:4.01|P-value:4.11E-6 ID:SGTC_1676|Compound:st018489|FD-Score:-3.13|P-value:2.49E-4 ID:SGTC_2068|Compound:5226020|FD-Score:-3.38|P-value:8.66E-5 ID:SGTC_2126|Compound:5251174|FD-Score:2.81|P-value:8.77E-4 ID:SGTC_2272|Compound:7805186|FD-Score:3.65|P-value:2.47E-5 ID:SGTC_2304|Compound:7443016|FD-Score:3.22|P-value:1.69E-4 ID:SGTC_2305|Compound:7443522|FD-Score:2.96|P-value:4.98E-4 ID:SGTC_2325|Compound:6158266|FD-Score:-3.65|P-value:2.44E-5 ID:SGTC_2449|Compound:5812982|FD-Score:2.93|P-value:5.52E-4 ID:SGTC_2740|Compound:felodipine|FD-Score:2.94|P-value:5.28E-4 ID:SGTC_2755|Compound:lomerizine|FD-Score:-3.99|P-value:4.62E-6 ID:SGTC_2854|Compound:9024559|FD-Score:3.41|P-value:7.54E-5 ID:SGTC_2855|Compound:9016525|FD-Score:3.07|P-value:3.22E-4 ID:SGTC_2858|Compound:9025781|FD-Score:3.76|P-value:1.47E-5 ID:SGTC_2893|Compound:9048488|FD-Score:-3.01|P-value:4.03E-4 ID:SGTC_3205|Compound:9113483|FD-Score:3.70|P-value:1.94E-5 ID:SGTC_3212|Compound:9128307|FD-Score:-2.78|P-value:9.73E-4 ID:SGTC_3264|Compound:9137442|FD-Score:2.85|P-value:7.61E-4 ID:SGTC_3265|Compound:9137266|FD-Score:2.87|P-value:7.19E-4 ID:SGTC_3266|Compound:9136860|FD-Score:3.16|P-value:2.22E-4 ID:SGTC_3277|Compound:9138724|FD-Score:3.35|P-value:9.85E-5 ID:SGTC_3304|Compound:9125678|FD-Score:5.46|P-value:6.37E-10 ID:SGTC_3311|Compound:9131146|FD-Score:-2.85|P-value:7.49E-4

Top fitness defect scores for YGL049C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_3304 9125678 5.46 46.1 µM 6.37E-10 16 2.42E-8
2 SGTC_1105 epirubicin 4.56 18.1 µM 1.96E-7 anthracycline transcription coupled DNA repair 8 2.56E-6
3 SGTC_532 1548-0774 4.07 215.0 µM 2.93E-6 15 2.31E-5
4 SGTC_115 0527-0192 4.03 112.0 µM 3.62E-6 64 2.75E-5
5 SGTC_1618 st003135 4.01 44.5 µM 4.11E-6 18 3.05E-5
6 SGTC_1050 3232-1156 3.93 69.3 µM 6.07E-6 RSC complex & mRNA processing 20 4.19E-5
7 SGTC_2858 9025781 3.76 9.0 µM 1.47E-5 sphingolipid biosynthesis & PDR1 107 8.63E-5
8 SGTC_3205 9113483 3.70 49.5 µM 1.94E-5 36 1.08E-4
9 SGTC_2272 7805186 3.65 180.0 µM 2.47E-5 cell wall signaling 26 1.32E-4
10 SGTC_2854 9024559 3.41 71.4 µM 7.54E-5 43 3.28E-4
11 SGTC_3277 9138724 3.35 49.5 µM 9.85E-5 Golgi 37 4.09E-4
12 SGTC_87 0100-0052 3.32 35.5 µM 1.13E-4 171 4.57E-4
13 SGTC_453 enantio-paf c16 3.24 10.0 µM 1.58E-4 213 6.01E-4
14 SGTC_2304 7443016 3.22 91.4 µM 1.69E-4 44 6.35E-4
15 SGTC_1100 amiodarone 3.22 5.1 µM 1.73E-4 42 6.47E-4
16 SGTC_3266 9136860 3.16 49.5 µM 2.22E-4 cell wall 78 7.94E-4
17 SGTC_414 piperonyl butoxide 3.15 100.0 µM 2.28E-4 29 8.11E-4
18 SGTC_2855 9016525 3.07 58.4 µM 3.22E-4 59 0.00108
19 SGTC_2305 7443522 2.96 69.2 µM 4.98E-4 50 0.00154
20 SGTC_2740 felodipine 2.94 17.1 µM 5.28E-4 53 0.00162

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.217 5.05E-37 YGL155W CDC43 het Beta subunit of geranylgeranyltransferase type I, catalyzes geranylgeranylation to the cysteine residue in proteins containing a C-terminal CaaX sequence ending in Leu or Phe; has substrates important for morphogenesis
0.197 9.61E-31 YKL019W RAM2 het Alpha subunit of both the farnesyltransferase and type I geranylgeranyltransferase that catalyze prenylation of proteins containing a CAAX consensus motif; essential protein required for membrane localization of Ras proteins and a-factor
0.189 3.51E-28 YDR309C GIC2 hom Redundant rho-like GTPase Cdc42p effector; homolog of Gic1p; involved in initiation of budding and cellular polarization; interacts with Cdc42p via the Cdc42/Rac-interactive binding (CRIB) domain and with PI(4,5)P2 via a polybasic region
0.187 1.09E-27 YKL058W TOA2 het TFIIA small subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to smallest subunit of human and Drosophila TFIIA; protein abundance increases in response to DNA replication stress
0.182 2.52E-26 YLR153C ACS2 het Acetyl-coA synthetase isoform which, along with Acs1p, is the nuclear source of acetyl-coA for histone acetylation; mutants affect global transcription; required for growth on glucose; expressed under anaerobic conditions
0.172 9.29E-24 YLR229C CDC42 het Small rho-like GTPase, essential for establishment and maintenance of cell polarity; mutants have defects in the organization of actin and septins
0.130 5.11E-14 YGL066W SGF73 hom SAGA complex subunit; has a role in anchoring the deubiquitination module into SAGA and SLIK complexes; involved in preinitiation complex assembly at promoters; human ortholog ataxin-7 is associated with spinocerebellar ataxia diseases; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay
0.127 1.29E-13 YLR230W_d YLR230W_d het Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential CDC42 gene which encodes a small Rho-like GTPase essential for establishment and maintenance of cell polarity
0.111 9.83E-11 YML101C-A_d YML101C-A_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.109 2.80E-10 YBL032W HEK2 hom RNA binding protein involved in the asymmetric localization of ASH1 mRNA; represses translation of ASH1 mRNA, an effect reversed by Yck1p-dependent phosphoryation; regulates telomere position effect and length; similarity to hnRNP-K
0.108 3.96E-10 YDR150W NUM1 hom Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex
0.107 4.48E-10 YLL001W DNM1 hom Dynamin-related GTPase required for mitochondrial fission and morphology; assembles on the cytoplasmic face of mitochondrial tubules at sites at which division will occur; also participates in endocytosis and regulating peroxisome abundance
0.107 5.66E-10 YDL072C YET3 hom Protein of unknown function; YET3 null mutant decreases the level of secreted invertase; homolog of human BAP31 protein; protein abundance increases in response to DNA replication stress
0.102 3.14E-9 YNL134C YNL134C hom Protein of unknown function; similar to dehydrogenases from other model organisms; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress
0.102 3.19E-9 YLR055C SPT8 hom Subunit of the SAGA transcriptional regulatory complex but not present in SAGA-like complex SLIK/SALSA, required for SAGA-mediated inhibition at some promoters