YGL050W / TYW3

tRNA methyltransferase required for synthesis of wybutosine, a modified guanosine found at the 3'-position adjacent to the anticodon of phenylalanine tRNA which supports reading frame maintenance by stabilizing codon-anticodon interactions

Zygosity: Homozygous strain
fixedexpanded
Profile for YGL050W / TYW3

Click on Significant Values for Screen Details ID:SGTC_174|Compound:3553-0389|FD-Score:3.68|P-value:9.37E-5 ID:SGTC_245|Compound:methotrexate|FD-Score:3.07|P-value:9.08E-4 ID:SGTC_349|Compound:k015-0014|FD-Score:3.83|P-value:5.03E-5 ID:SGTC_438|Compound:sphingosine|FD-Score:3.12|P-value:7.76E-4 ID:SGTC_622|Compound:1348-1566|FD-Score:-3.46|P-value:2.15E-4 ID:SGTC_636|Compound:0kpi-0074|FD-Score:5.61|P-value:6.11E-9 ID:SGTC_649|Compound:1123-0311|FD-Score:-3.46|P-value:2.10E-4 ID:SGTC_799|Compound:1310-0557|FD-Score:6.27|P-value:9.51E-11 ID:SGTC_1033|Compound:k072-0102|FD-Score:3.49|P-value:1.97E-4 ID:SGTC_1168|Compound:k815-0004|FD-Score:3.25|P-value:4.94E-4 ID:SGTC_1202|Compound:1988-0068|FD-Score:4.37|P-value:4.46E-6 ID:SGTC_1372|Compound:2752-0194|FD-Score:5.92|P-value:8.81E-10 ID:SGTC_1413|Compound:3910-0535|FD-Score:3.26|P-value:4.61E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-3.26|P-value:4.54E-4 ID:SGTC_1529|Compound:apigenin|FD-Score:3.46|P-value:2.25E-4 ID:SGTC_1559|Compound:coumarin 343|FD-Score:3.18|P-value:6.36E-4 ID:SGTC_1567|Compound:st069303|FD-Score:-3.12|P-value:7.30E-4 ID:SGTC_1594|Compound:piperine|FD-Score:4.30|P-value:6.40E-6 ID:SGTC_1713|Compound:st033146|FD-Score:4.56|P-value:1.85E-6 ID:SGTC_1733|Compound:st037357|FD-Score:3.41|P-value:2.65E-4 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:3.49|P-value:2.00E-4 ID:SGTC_1856|Compound:st057054|FD-Score:3.53|P-value:1.67E-4 ID:SGTC_1858|Compound:4-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione|FD-Score:4.59|P-value:1.54E-6 ID:SGTC_1966|Compound:st070145|FD-Score:-3.05|P-value:9.47E-4 ID:SGTC_2005|Compound:4024620|FD-Score:-3.05|P-value:9.39E-4 ID:SGTC_2166|Compound:5933061|FD-Score:3.46|P-value:2.21E-4 ID:SGTC_2209|Compound:5-(5-methyl-2-thenylidene)-Barbituric acid|FD-Score:3.05|P-value:9.95E-4 ID:SGTC_2238|Compound:6671620|FD-Score:-5.15|P-value:7.85E-8 ID:SGTC_2241|Compound:6772625|FD-Score:-3.64|P-value:1.05E-4 ID:SGTC_2330|Compound:7979373|FD-Score:3.23|P-value:5.30E-4 ID:SGTC_2336|Compound:9001819|FD-Score:-3.14|P-value:6.97E-4 ID:SGTC_2449|Compound:5812982|FD-Score:3.29|P-value:4.27E-4 ID:SGTC_2453|Compound:5731132|FD-Score:-4.92|P-value:2.73E-7 ID:SGTC_2695|Compound:st077686|FD-Score:4.74|P-value:7.55E-7 ID:SGTC_2751|Compound:anetholtrithion|FD-Score:-4.46|P-value:2.84E-6 ID:SGTC_2761|Compound:levamisole|FD-Score:3.27|P-value:4.57E-4 ID:SGTC_2959|Compound:9079155|FD-Score:6.56|P-value:1.33E-11 ID:SGTC_2962|Compound:9082311|FD-Score:-3.37|P-value:3.03E-4 ID:SGTC_174|Compound:3553-0389|FD-Score:3.68|P-value:9.37E-5 ID:SGTC_245|Compound:methotrexate|FD-Score:3.07|P-value:9.08E-4 ID:SGTC_349|Compound:k015-0014|FD-Score:3.83|P-value:5.03E-5 ID:SGTC_438|Compound:sphingosine|FD-Score:3.12|P-value:7.76E-4 ID:SGTC_622|Compound:1348-1566|FD-Score:-3.46|P-value:2.15E-4 ID:SGTC_636|Compound:0kpi-0074|FD-Score:5.61|P-value:6.11E-9 ID:SGTC_649|Compound:1123-0311|FD-Score:-3.46|P-value:2.10E-4 ID:SGTC_799|Compound:1310-0557|FD-Score:6.27|P-value:9.51E-11 ID:SGTC_1033|Compound:k072-0102|FD-Score:3.49|P-value:1.97E-4 ID:SGTC_1168|Compound:k815-0004|FD-Score:3.25|P-value:4.94E-4 ID:SGTC_1202|Compound:1988-0068|FD-Score:4.37|P-value:4.46E-6 ID:SGTC_1372|Compound:2752-0194|FD-Score:5.92|P-value:8.81E-10 ID:SGTC_1413|Compound:3910-0535|FD-Score:3.26|P-value:4.61E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-3.26|P-value:4.54E-4 ID:SGTC_1529|Compound:apigenin|FD-Score:3.46|P-value:2.25E-4 ID:SGTC_1559|Compound:coumarin 343|FD-Score:3.18|P-value:6.36E-4 ID:SGTC_1567|Compound:st069303|FD-Score:-3.12|P-value:7.30E-4 ID:SGTC_1594|Compound:piperine|FD-Score:4.30|P-value:6.40E-6 ID:SGTC_1713|Compound:st033146|FD-Score:4.56|P-value:1.85E-6 ID:SGTC_1733|Compound:st037357|FD-Score:3.41|P-value:2.65E-4 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:3.49|P-value:2.00E-4 ID:SGTC_1856|Compound:st057054|FD-Score:3.53|P-value:1.67E-4 ID:SGTC_1858|Compound:4-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione|FD-Score:4.59|P-value:1.54E-6 ID:SGTC_1966|Compound:st070145|FD-Score:-3.05|P-value:9.47E-4 ID:SGTC_2005|Compound:4024620|FD-Score:-3.05|P-value:9.39E-4 ID:SGTC_2166|Compound:5933061|FD-Score:3.46|P-value:2.21E-4 ID:SGTC_2209|Compound:5-(5-methyl-2-thenylidene)-Barbituric acid|FD-Score:3.05|P-value:9.95E-4 ID:SGTC_2238|Compound:6671620|FD-Score:-5.15|P-value:7.85E-8 ID:SGTC_2241|Compound:6772625|FD-Score:-3.64|P-value:1.05E-4 ID:SGTC_2330|Compound:7979373|FD-Score:3.23|P-value:5.30E-4 ID:SGTC_2336|Compound:9001819|FD-Score:-3.14|P-value:6.97E-4 ID:SGTC_2449|Compound:5812982|FD-Score:3.29|P-value:4.27E-4 ID:SGTC_2453|Compound:5731132|FD-Score:-4.92|P-value:2.73E-7 ID:SGTC_2695|Compound:st077686|FD-Score:4.74|P-value:7.55E-7 ID:SGTC_2751|Compound:anetholtrithion|FD-Score:-4.46|P-value:2.84E-6 ID:SGTC_2761|Compound:levamisole|FD-Score:3.27|P-value:4.57E-4 ID:SGTC_2959|Compound:9079155|FD-Score:6.56|P-value:1.33E-11 ID:SGTC_2962|Compound:9082311|FD-Score:-3.37|P-value:3.03E-4

Top fitness defect scores for YGL050W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2959 9079155 6.56 11.0 µM 1.33E-11 4 2.70E-11
2 SGTC_799 1310-0557 6.27 151.0 µM 9.51E-11 superoxide 6 1.81E-10
3 SGTC_1372 2752-0194 5.92 270.0 µM 8.81E-10 4 1.56E-9
4 SGTC_636 0kpi-0074 5.61 126.0 µM 6.11E-9 7 1.02E-8
5 SGTC_2695 st077686 4.74 52.0 µM 7.55E-7 17 1.09E-6
6 SGTC_1858 4-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione 4.59 62.6 µM 1.54E-6 TSC3-RPN4 8 2.17E-6
7 SGTC_1713 st033146 4.56 12.8 µM 1.85E-6 15 2.60E-6
8 SGTC_1202 1988-0068 4.37 29.6 µM 4.46E-6 RNA processing & uracil transport 17 6.08E-6
9 SGTC_1594 piperine 4.30 70.1 µM 6.40E-6 8 8.63E-6
10 SGTC_349 k015-0014 3.83 24.0 µM 5.03E-5 20 6.38E-5
11 SGTC_174 3553-0389 3.68 74.3 µM 9.37E-5 29 1.17E-4
12 SGTC_1856 st057054 3.53 55.7 µM 1.67E-4 36 2.04E-4
13 SGTC_1033 k072-0102 3.49 21.3 µM 1.97E-4 60S ribosome export 37 2.40E-4
14 SGTC_1785 bathophenanthroline 3.49 24.0 µM 2.00E-4 heme requiring 69 2.43E-4
15 SGTC_2166 5933061 3.46 31.5 µM 2.21E-4 tubulin folding & SWR complex 57 2.68E-4
16 SGTC_1529 apigenin 3.46 74.0 µM 2.25E-4 51 2.73E-4
17 SGTC_1733 st037357 3.41 54.9 µM 2.65E-4 60S ribosome export 61 3.20E-4
18 SGTC_2449 5812982 3.29 45.8 µM 4.27E-4 25 5.08E-4
19 SGTC_2761 levamisole 3.27 97.9 µM 4.57E-4 37 5.43E-4
20 SGTC_1413 3910-0535 3.26 34.4 µM 4.61E-4 33 5.47E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.164 1.33E-21 YDR107C TMN2 hom Protein with a role in cellular adhesion and filamentous growth; similar to Emp70p and Tmn3p; member of the evolutionarily conserved Transmembrane Nine family of proteins with nine membrane-spanning segments
0.152 7.30E-19 YGR022C_d YGR022C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF MTL1/YGR023W
0.136 2.80E-15 YBL017C PEP1 hom Type I transmembrane sorting receptor for multiple vacuolar hydrolases; cycles between the late-Golgi and prevacuolar endosome-like compartments
0.121 2.44E-12 YDR294C DPL1 hom Dihydrosphingosine phosphate lyase, regulates intracellular levels of sphingolipid long-chain base phosphates (LCBPs), degrades phosphorylated long chain bases, prefers C16 dihydrosphingosine-l-phosphate as a substrate
0.109 2.74E-10 YGR292W MAL12 hom Maltase (alpha-D-glucosidase), inducible protein involved in maltose catabolism; encoded in the MAL1 complex locus; hydrolyzes the disaccharides maltose, turanose, maltotriose, and sucrose
0.107 4.83E-10 YDR109C_p YDR109C_p hom Putative kinase
0.098 1.28E-8 YOR114W_p YOR114W_p hom Putative protein of unknown function; null mutant is viable
0.092 8.95E-8 YOR017W PET127 hom Protein with a role in 5'-end processing of mitochondrial RNAs, located in the mitochondrial membrane
0.092 1.01E-7 YOL101C IZH4 hom Membrane protein involved in zinc ion homeostasis; member of the four-protein IZH family; expression induced by fatty acids and altered zinc levels; deletion reduces sensitivity to excess zinc; possible role in sterol metabolism; protein increases in abundance and relocalizes from nucleus to ER upon DNA replication stress
0.091 1.27E-7 YDL086W_p YDL086W_p hom Putative carboxymethylenebutenolidase; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YDL086W is not an essential gene
0.089 2.10E-7 YBL001C ECM15 hom Non-essential protein of unknown function, likely exists as tetramer, may be regulated by the binding of small-molecule ligands (possibly sulfate ions), may have a role in yeast cell-wall biogenesis
0.089 2.71E-7 YKR102W FLO10 hom Member of the FLO family of cell wall flocculation proteins; not expressed in most lab strains; overproduction induces flocculation that can be inhibited by mannose, sucrose, or glucose; overproduction also promotes haploid invasive growth and diploid filamentous growth
0.088 2.96E-7 YJL051W IRC8 hom Bud tip localized protein of unknown function; mRNA is targeted to the bud by a She2p dependent transport system; mRNA is cell cycle regulated via Fkh2p, peaking in G2/M phase; null mutant displays increased levels of spontaneous Rad52p foc
0.087 4.44E-7 YOL060C MAM3 hom Protein required for normal mitochondrial morphology, has similarity to hemolysins
0.087 4.81E-7 YDL222C FMP45 hom Integral membrane protein localized to mitochondria (untagged protein); required for sporulation and maintaining sphingolipid content; has sequence similarity to SUR7 and YNL194C