HIPHOP chemogenomics database

YGL063W / PUS2

Mitochondrial tRNA:pseudouridine synthase; acts at positions 27 and 28, but not at position 72; efficiently and rapidly targeted to mitochondria, specifically dedicated to mitochondrial tRNA modification

Zygosity: Homozygous strain

fixedexpanded
Profile for YGL063W / PUS2

Click on Significant Values for Screen Details

Top fitness defect scores for YGL063W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_2592	piperlongumine	8.13	83.3 µM	3.66E-16	TSC3-RPN4	3	2.08E-16
2	SGTC_69	0096-0274	7.05	206.7 µM	1.35E-12	mitochondrial processes	6	9.04E-13
3	SGTC_1146	3579-1023	6.46	7.0 µM	7.44E-11		3	5.38E-11
4	SGTC_1315	1237-0019	6.13	3.7 µM	5.73E-10		1	4.31E-10
5	SGTC_2060	5238662	6.09	140.0 µM	7.38E-10		4	5.58E-10
6	SGTC_939	1165-0479	5.34	45.3 µM	5.73E-8		6	4.70E-8
7	SGTC_1845	st056753	5.18	3.0 µM	1.32E-7	TSC3-RPN4	17	1.10E-7
8	SGTC_2394	5221648	5.12	200.0 µM	1.79E-7		9	1.49E-7
9	SGTC_588	3970-1144	4.66	52.9 µM	1.82E-6		15	1.59E-6
10	SGTC_516	3992-2719	4.60	87.3 µM	2.44E-6		10	2.14E-6
11	SGTC_657	3534-0529	4.52	86.7 µM	3.49E-6		7	3.08E-6
12	SGTC_2055	5235184	4.45	200.0 µM	4.76E-6	ERG2	19	4.22E-6
13	SGTC_951	1171-0579	4.27	82.7 µM	1.08E-5	cell wall	63	9.70E-6
14	SGTC_1785	bathophenanthroline	4.20	24.0 µM	1.45E-5	heme requiring	39	1.31E-5
15	SGTC_643	k048-0106	4.06	28.4 µM	2.66E-5	RSC & ERG11	10	2.43E-5
16	SGTC_2826	7992541	4.03	19.5 µM	3.02E-5		18	2.76E-5
17	SGTC_1908	st060353	3.91	56.5 µM	4.91E-5	RPP1 & pyrimidine depletion	37	4.52E-5
18	SGTC_3181	9108277	3.81	49.5 µM	7.45E-5	iron homeostasis	21	6.91E-5
19	SGTC_2418	pentamidine	3.80	31.7 µM	7.71E-5	mitochondrial processes	50	7.16E-5
20	SGTC_1977	st070720	3.76	65.3 µM	9.14E-5		13	8.51E-5

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.106	7.25E-10	YMR254C_d	YMR254C_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.095	2.98E-8	YLR297W	YLR297W	hom	Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; not an essential gene; induced by treatment with 8-methoxypsoralen and UVA irradiation; relocalizes from nucleus to vacuole upon DNA replication stress; YLR297W has a paralog, YOR186W, that arose from the whole genome duplication
0.091	1.28E-7	YGL062W	PYC1	hom	Pyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentially regulated than isoform Pyc2p; mutations in the human homolog are associated with lactic acidosis; PYC1 has a paralog, PYC2, that arose from the whole genome duplication
0.089	2.34E-7	YDL026W_d	YDL026W_d	hom	Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data
0.089	2.68E-7	YOR282W_d	YOR282W_d	het	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps essential, verified gene PLP2/YOR281C
0.089	2.79E-7	YDL178W	DLD2	hom	D-lactate dehydrogenase, located in the mitochondrial matrix
0.084	1.24E-6	YNL059C	ARP5	hom	Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes
0.083	1.41E-6	YPL211W	NIP7	het	Nucleolar protein required for 60S ribosome subunit biogenesis, constituent of 66S pre-ribosomal particles; physically interacts with Nop8p and the exosome subunit Rrp43p
0.083	1.63E-6	YBR265W	TSC10	het	3-ketosphinganine reductase, catalyzes the second step in phytosphingosine synthesis, essential for growth in the absence of exogenous dihydrosphingosine or phytosphingosine, member of short chain dehydrogenase/reductase protein family
0.081	2.32E-6	YKL006C-A	SFT1	het	Intra-Golgi v-SNARE, required for transport of proteins between an early and a later Golgi compartment
0.081	2.62E-6	YER123W	YCK3	hom	Palmitoylated, vacuolar membrane-localized casein kinase I isoform; negatively regulates vacuole fusion during hypertonic stress via phosphorylation of Vps41p; shares essential functions with Hrr25p; regulates vesicle fusion in AP-3 pathway
0.077	8.41E-6	YMR062C	ARG7	hom	Mitochondrial ornithine acetyltransferase, catalyzes the fifth step in arginine biosynthesis; also possesses acetylglutamate synthase activity, regenerates acetylglutamate while forming ornithine
0.076	1.13E-5	YKL043W	PHD1	hom	Transcriptional activator that enhances pseudohyphal growth; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; regulates expression of FLO11, an adhesin required for pseudohyphal filament formation; similar to StuA, an A. nidulans developmental regulator; potential Cdc28p substrate; PHD1 has a paralog, SOK2, that arose from the whole genome duplication
0.076	1.15E-5	YIL164C	NIT1	hom	Nitrilase, member of the nitrilase branch of the nitrilase superfamily; in closely related species and other S. cerevisiae strain backgrounds YIL164C and adjacent ORF, YIL165C, likely constitute a single ORF encoding a nitrilase gene
0.076	1.25E-5	YOR092W	ECM3	hom	Non-essential protein of unknown function; involved in signal transduction and the genotoxic response; induced rapidly in response to treatment with 8-methoxypsoralen and UVA irradiation; relocalizes from ER to cytoplasm upon DNA replication stress; ECM3 has a paralog, YNL095C, that arose from the whole genome duplication