YGL065C / ALG2

Mannosyltransferase that catalyzes two consecutive steps in the N-linked glycosylation pathway; alg2 mutants exhibit temperature-sensitive growth and abnormal accumulation of the lipid-linked oligosaccharide Man2GlcNAc2-PP-Dol

Zygosity: Heterozygous strain
fixedexpanded
Profile for YGL065C / ALG2

Click on Significant Values for Screen Details ID:SGTC_36|Compound:0335-0881|FD-Score:-3.79|P-value:4.11E-5 ID:SGTC_41|Compound:0478-0632|FD-Score:3.15|P-value:5.80E-4 ID:SGTC_64|Compound:000l-0018|FD-Score:-3.29|P-value:3.11E-4 ID:SGTC_137|Compound:0438-0332|FD-Score:-3.00|P-value:9.18E-4 ID:SGTC_212|Compound:3807-4682|FD-Score:8.81|P-value:4.44E-20 ID:SGTC_229|Compound:benomyl|FD-Score:3.65|P-value:8.28E-5 ID:SGTC_288|Compound:1315-0019|FD-Score:3.25|P-value:4.02E-4 ID:SGTC_291|Compound:4130-1278|FD-Score:3.91|P-value:2.74E-5 ID:SGTC_395|Compound:anisomycin|FD-Score:3.06|P-value:8.04E-4 ID:SGTC_464|Compound:bw-b 70c|FD-Score:3.05|P-value:8.41E-4 ID:SGTC_696|Compound:1560-0013|FD-Score:3.05|P-value:8.44E-4 ID:SGTC_755|Compound:1995-0008|FD-Score:3.12|P-value:6.63E-4 ID:SGTC_879|Compound:0831-1030|FD-Score:3.11|P-value:6.83E-4 ID:SGTC_1001|Compound:4048-4355|FD-Score:-3.71|P-value:5.81E-5 ID:SGTC_1003|Compound:4049-0218|FD-Score:-3.84|P-value:3.34E-5 ID:SGTC_1036|Compound:k216-0215|FD-Score:-3.20|P-value:4.41E-4 ID:SGTC_1238|Compound:0333-0287|FD-Score:-3.23|P-value:3.87E-4 ID:SGTC_1293|Compound:1000-1278|FD-Score:3.89|P-value:3.02E-5 ID:SGTC_1304|Compound:1165-0502|FD-Score:5.72|P-value:1.77E-9 ID:SGTC_1310|Compound:1188-0297|FD-Score:3.15|P-value:5.91E-4 ID:SGTC_1393|Compound:3470-5649|FD-Score:3.23|P-value:4.37E-4 ID:SGTC_1469|Compound:k284-2957|FD-Score:-4.52|P-value:1.36E-6 ID:SGTC_1486|Compound:3970-0793|FD-Score:3.00|P-value:9.89E-4 ID:SGTC_1675|Compound:st018485|FD-Score:-3.10|P-value:6.27E-4 ID:SGTC_1679|Compound:4130-1276|FD-Score:10.50|P-value:1.34E-27 ID:SGTC_1727|Compound:st034309|FD-Score:3.18|P-value:5.30E-4 ID:SGTC_1734|Compound:st037168|FD-Score:-4.19|P-value:6.84E-6 ID:SGTC_1735|Compound:st034310|FD-Score:4.28|P-value:5.13E-6 ID:SGTC_1769|Compound:st044406|FD-Score:-3.11|P-value:6.15E-4 ID:SGTC_1788|Compound:fluconazole|FD-Score:3.14|P-value:6.14E-4 ID:SGTC_1875|Compound:nocodazole|FD-Score:3.76|P-value:5.32E-5 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:-3.08|P-value:6.90E-4 ID:SGTC_1928|Compound:4'-methoxychalcone|FD-Score:3.88|P-value:3.22E-5 ID:SGTC_1929|Compound:genistein trimethyl ether|FD-Score:4.29|P-value:4.86E-6 ID:SGTC_1932|Compound:st074806|FD-Score:-3.63|P-value:8.25E-5 ID:SGTC_2054|Compound:5233173|FD-Score:-4.52|P-value:1.33E-6 ID:SGTC_2131|Compound:toluylene red|FD-Score:-3.38|P-value:2.23E-4 ID:SGTC_2158|Compound:5657123|FD-Score:-3.92|P-value:2.32E-5 ID:SGTC_2174|Compound:5790901|FD-Score:3.39|P-value:2.35E-4 ID:SGTC_2260|Compound:7964312|FD-Score:3.29|P-value:3.56E-4 ID:SGTC_2303|Compound:7774051|FD-Score:3.06|P-value:7.98E-4 ID:SGTC_2338|Compound:7999974|FD-Score:3.08|P-value:7.47E-4 ID:SGTC_2434|Compound:5670830|FD-Score:-3.01|P-value:8.70E-4 ID:SGTC_2527|Compound:2',3'-dihydroxy-4-methoxy-4'-ethoxybenzophenone|FD-Score:8.03|P-value:5.44E-17 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:3.98|P-value:2.00E-5 ID:SGTC_2596|Compound:sinensetin|FD-Score:-3.12|P-value:5.97E-4 ID:SGTC_2620|Compound:irigenin|FD-Score:3.69|P-value:7.09E-5 ID:SGTC_2688|Compound:kanamycin b|FD-Score:-5.08|P-value:6.81E-8 ID:SGTC_2690|Compound:usnic acid|FD-Score:3.33|P-value:3.02E-4 ID:SGTC_2695|Compound:st077686|FD-Score:3.37|P-value:2.59E-4 ID:SGTC_2823|Compound:7987624|FD-Score:3.21|P-value:4.63E-4 ID:SGTC_2824|Compound:7998404|FD-Score:4.62|P-value:9.24E-7 ID:SGTC_2882|Compound:9058044|FD-Score:4.21|P-value:7.13E-6 ID:SGTC_2890|Compound:9046729|FD-Score:-3.17|P-value:4.99E-4 ID:SGTC_3046|Compound:9092212|FD-Score:3.57|P-value:1.15E-4 ID:SGTC_3095|Compound:9117207|FD-Score:-3.18|P-value:4.74E-4 ID:SGTC_3149|Compound:9097855|FD-Score:3.05|P-value:8.46E-4 ID:SGTC_3291|Compound:9121177|FD-Score:3.43|P-value:2.00E-4 ID:SGTC_3298|Compound:9116858|FD-Score:3.94|P-value:2.38E-5 ID:SGTC_3312|Compound:9133330|FD-Score:4.48|P-value:1.87E-6 ID:SGTC_36|Compound:0335-0881|FD-Score:-3.79|P-value:4.11E-5 ID:SGTC_41|Compound:0478-0632|FD-Score:3.15|P-value:5.80E-4 ID:SGTC_64|Compound:000l-0018|FD-Score:-3.29|P-value:3.11E-4 ID:SGTC_137|Compound:0438-0332|FD-Score:-3.00|P-value:9.18E-4 ID:SGTC_212|Compound:3807-4682|FD-Score:8.81|P-value:4.44E-20 ID:SGTC_229|Compound:benomyl|FD-Score:3.65|P-value:8.28E-5 ID:SGTC_288|Compound:1315-0019|FD-Score:3.25|P-value:4.02E-4 ID:SGTC_291|Compound:4130-1278|FD-Score:3.91|P-value:2.74E-5 ID:SGTC_395|Compound:anisomycin|FD-Score:3.06|P-value:8.04E-4 ID:SGTC_464|Compound:bw-b 70c|FD-Score:3.05|P-value:8.41E-4 ID:SGTC_696|Compound:1560-0013|FD-Score:3.05|P-value:8.44E-4 ID:SGTC_755|Compound:1995-0008|FD-Score:3.12|P-value:6.63E-4 ID:SGTC_879|Compound:0831-1030|FD-Score:3.11|P-value:6.83E-4 ID:SGTC_1001|Compound:4048-4355|FD-Score:-3.71|P-value:5.81E-5 ID:SGTC_1003|Compound:4049-0218|FD-Score:-3.84|P-value:3.34E-5 ID:SGTC_1036|Compound:k216-0215|FD-Score:-3.20|P-value:4.41E-4 ID:SGTC_1238|Compound:0333-0287|FD-Score:-3.23|P-value:3.87E-4 ID:SGTC_1293|Compound:1000-1278|FD-Score:3.89|P-value:3.02E-5 ID:SGTC_1304|Compound:1165-0502|FD-Score:5.72|P-value:1.77E-9 ID:SGTC_1310|Compound:1188-0297|FD-Score:3.15|P-value:5.91E-4 ID:SGTC_1393|Compound:3470-5649|FD-Score:3.23|P-value:4.37E-4 ID:SGTC_1469|Compound:k284-2957|FD-Score:-4.52|P-value:1.36E-6 ID:SGTC_1486|Compound:3970-0793|FD-Score:3.00|P-value:9.89E-4 ID:SGTC_1675|Compound:st018485|FD-Score:-3.10|P-value:6.27E-4 ID:SGTC_1679|Compound:4130-1276|FD-Score:10.50|P-value:1.34E-27 ID:SGTC_1727|Compound:st034309|FD-Score:3.18|P-value:5.30E-4 ID:SGTC_1734|Compound:st037168|FD-Score:-4.19|P-value:6.84E-6 ID:SGTC_1735|Compound:st034310|FD-Score:4.28|P-value:5.13E-6 ID:SGTC_1769|Compound:st044406|FD-Score:-3.11|P-value:6.15E-4 ID:SGTC_1788|Compound:fluconazole|FD-Score:3.14|P-value:6.14E-4 ID:SGTC_1875|Compound:nocodazole|FD-Score:3.76|P-value:5.32E-5 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:-3.08|P-value:6.90E-4 ID:SGTC_1928|Compound:4'-methoxychalcone|FD-Score:3.88|P-value:3.22E-5 ID:SGTC_1929|Compound:genistein trimethyl ether|FD-Score:4.29|P-value:4.86E-6 ID:SGTC_1932|Compound:st074806|FD-Score:-3.63|P-value:8.25E-5 ID:SGTC_2054|Compound:5233173|FD-Score:-4.52|P-value:1.33E-6 ID:SGTC_2131|Compound:toluylene red|FD-Score:-3.38|P-value:2.23E-4 ID:SGTC_2158|Compound:5657123|FD-Score:-3.92|P-value:2.32E-5 ID:SGTC_2174|Compound:5790901|FD-Score:3.39|P-value:2.35E-4 ID:SGTC_2260|Compound:7964312|FD-Score:3.29|P-value:3.56E-4 ID:SGTC_2303|Compound:7774051|FD-Score:3.06|P-value:7.98E-4 ID:SGTC_2338|Compound:7999974|FD-Score:3.08|P-value:7.47E-4 ID:SGTC_2434|Compound:5670830|FD-Score:-3.01|P-value:8.70E-4 ID:SGTC_2527|Compound:2',3'-dihydroxy-4-methoxy-4'-ethoxybenzophenone|FD-Score:8.03|P-value:5.44E-17 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:3.98|P-value:2.00E-5 ID:SGTC_2596|Compound:sinensetin|FD-Score:-3.12|P-value:5.97E-4 ID:SGTC_2620|Compound:irigenin|FD-Score:3.69|P-value:7.09E-5 ID:SGTC_2688|Compound:kanamycin b|FD-Score:-5.08|P-value:6.81E-8 ID:SGTC_2690|Compound:usnic acid|FD-Score:3.33|P-value:3.02E-4 ID:SGTC_2695|Compound:st077686|FD-Score:3.37|P-value:2.59E-4 ID:SGTC_2823|Compound:7987624|FD-Score:3.21|P-value:4.63E-4 ID:SGTC_2824|Compound:7998404|FD-Score:4.62|P-value:9.24E-7 ID:SGTC_2882|Compound:9058044|FD-Score:4.21|P-value:7.13E-6 ID:SGTC_2890|Compound:9046729|FD-Score:-3.17|P-value:4.99E-4 ID:SGTC_3046|Compound:9092212|FD-Score:3.57|P-value:1.15E-4 ID:SGTC_3095|Compound:9117207|FD-Score:-3.18|P-value:4.74E-4 ID:SGTC_3149|Compound:9097855|FD-Score:3.05|P-value:8.46E-4 ID:SGTC_3291|Compound:9121177|FD-Score:3.43|P-value:2.00E-4 ID:SGTC_3298|Compound:9116858|FD-Score:3.94|P-value:2.38E-5 ID:SGTC_3312|Compound:9133330|FD-Score:4.48|P-value:1.87E-6

Top fitness defect scores for YGL065C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_1679 4130-1276 10.50 6.7 µM 1.34E-27 Golgi 6 5.87E-26 0.34
2 SGTC_212 3807-4682 8.81 38.8 µM 4.44E-20 tubulin folding & SWR complex 6 6.36E-19 0.65
3 SGTC_2527 2',3'-dihydroxy-4-methoxy-4'-ethoxybenzophenone 8.03 63.6 µM 5.44E-17 1 4.94E-16 2.08
4 SGTC_1304 1165-0502 5.72 251.0 µM 1.77E-9 1 5.37E-9 1.00
5 SGTC_2824 7998404 4.62 52.0 µM 9.24E-7 6 1.90E-6 1.14
6 SGTC_3312 9133330 4.48 71.4 µM 1.87E-6 1 3.68E-6 0.31
7 SGTC_1929 genistein trimethyl ether 4.29 50.5 µM 4.86E-6 4 9.01E-6 0.48
8 SGTC_1735 st034310 4.28 56.8 µM 5.13E-6 Golgi 7 9.48E-6 0.14
9 SGTC_2882 9058044 4.21 58.4 µM 7.13E-6 2 1.29E-5 0.08
10 SGTC_2567 aureobasidin a 3.98 60.0 nM 2.00E-5 44 3.40E-5 0.05
11 SGTC_3298 9116858 3.94 71.4 µM 2.38E-5 3 4.01E-5 0.31
12 SGTC_291 4130-1278 3.91 35.5 µM 2.74E-5 Golgi 7 4.58E-5 0.23
13 SGTC_1293 1000-1278 3.89 195.0 µM 3.02E-5 6 5.02E-5 0.01
14 SGTC_1928 4'-methoxychalcone 3.88 40.3 µM 3.22E-5 6 5.32E-5 0.62
15 SGTC_1875 nocodazole 3.76 6.0 µM 5.32E-5 tubulin folding & SWR complex 23 8.54E-5 0.48
16 SGTC_2620 irigenin 3.69 100.0 µM 7.09E-5 18 1.12E-4 0.02
17 SGTC_229 benomyl 3.65 22.9 µM 8.28E-5 tubulin folding & SWR complex 14 1.29E-4 0.02
18 SGTC_3046 9092212 3.57 49.5 µM 1.15E-4 1 1.77E-4 0.20
19 SGTC_3291 9121177 3.43 71.4 µM 2.00E-4 2 2.97E-4 0.28
20 SGTC_2174 5790901 3.39 200.0 µM 2.35E-4 tubulin folding & SWR complex 13 3.45E-4 0.01

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.255 9.04E-51 YMR079W SEC14 het Phosphatidylinositol/phosphatidylcholine transfer protein; involved in regulating PtdIns, PtdCho, and ceramide metabolism, products of which regulate intracellular transport and UPR; has a role in localization of lipid raft proteins; functionally homologous to mammalian PITPs
0.181 3.71E-26 YBL034C STU1 het Component of the mitotic spindle that binds to interpolar microtubules via its association with beta-tubulin (Tub2p); required for interpolar microtubules to provide an outward force on the spindle poles
0.176 7.28E-25 YER007W PAC2 hom Microtubule effector required for tubulin heterodimer formation, binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomyl
0.147 1.36E-17 YGL094C PAN2 hom Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes
0.146 1.44E-17 YJL008C CCT8 het Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
0.145 3.44E-17 YJL085W EXO70 het Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in an actin-independent manner, targeting and anchoring the exocyst with Sec3p; involved in exocyst assembly; direct downstream effector of Rho3p and Cdc42p; relocalizes from bud neck to cytoplasm upon DNA replication stress
0.142 1.23E-16 YDR212W TCP1 het Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; involved in actin cytoskeleton maintenance; overexpression in neurons suppresses formation of pathogenic conformations of huntingtin protein
0.137 1.91E-15 YKL025C PAN3 hom Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes
0.133 9.04E-15 YPL241C CIN2 hom GTPase-activating protein (GAP) for Cin4p; tubulin folding factor C involved in beta-tubulin (Tub2p) folding; mutants display increased chromosome loss and benomyl sensitivity; deletion complemented by human GAP, retinitis pigmentosa 2
0.123 8.06E-13 YDR187C_d YDR187C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W
0.123 1.02E-12 YLR309C IMH1 hom Protein involved in vesicular transport, mediates transport between an endosomal compartment and the Golgi, contains a Golgi-localization (GRIP) domain that interacts with activated Arl1p-GTP to localize Imh1p to the Golgi
0.119 4.12E-12 YKL004W AUR1 het Phosphatidylinositol:ceramide phosphoinositol transferase (IPC synthase), required for sphingolipid synthesis; can mutate to confer aureobasidin A resistance
0.114 3.11E-11 YOR349W CIN1 hom Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl
0.112 7.12E-11 YIL142W CCT2 het Subunit beta of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
0.112 8.13E-11 YDR334W SWR1 hom Swi2/Snf2-related ATPase that is the structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A