YGL066W / SGF73

SAGA complex subunit; has a role in anchoring the deubiquitination module into SAGA and SLIK complexes; involved in preinitiation complex assembly at promoters; human ortholog ataxin-7 is associated with spinocerebellar ataxia diseases; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay

Zygosity: Homozygous strain
fixedexpanded
Profile for YGL066W / SGF73

Click on Significant Values for Screen Details ID:SGTC_58|Compound:0957-0393|FD-Score:8.90|P-value:2.79E-16 ID:SGTC_108|Compound:0509-0257|FD-Score:4.56|P-value:1.81E-5 ID:SGTC_115|Compound:0527-0192|FD-Score:6.43|P-value:2.55E-9 ID:SGTC_143|Compound:4032-1194|FD-Score:6.32|P-value:4.74E-9 ID:SGTC_150|Compound:3970-0969|FD-Score:4.47|P-value:2.52E-5 ID:SGTC_156|Compound:3474-0145|FD-Score:3.79|P-value:3.00E-4 ID:SGTC_168|Compound:3970-0862|FD-Score:5.13|P-value:1.67E-6 ID:SGTC_207|Compound:4185-0010|FD-Score:3.73|P-value:3.74E-4 ID:SGTC_266|Compound:formosanin C|FD-Score:9.79|P-value:2.63E-19 ID:SGTC_299|Compound:1273-0059|FD-Score:-4.62|P-value:1.04E-5 ID:SGTC_310|Compound:1273-0058|FD-Score:-6.44|P-value:1.59E-9 ID:SGTC_312|Compound:4092-0653|FD-Score:3.80|P-value:2.94E-4 ID:SGTC_314|Compound:1671-0008|FD-Score:-3.98|P-value:1.21E-4 ID:SGTC_421|Compound:streptovitacin|FD-Score:6.11|P-value:1.44E-8 ID:SGTC_431|Compound:k089-0097|FD-Score:-5.93|P-value:2.45E-8 ID:SGTC_513|Compound:4245-1575|FD-Score:-4.55|P-value:1.40E-5 ID:SGTC_536|Compound:3852-0578|FD-Score:3.84|P-value:2.60E-4 ID:SGTC_664|Compound:1222-0054|FD-Score:4.24|P-value:6.24E-5 ID:SGTC_698|Compound:k064-0052|FD-Score:3.57|P-value:6.24E-4 ID:SGTC_885|Compound:0865-0106|FD-Score:3.74|P-value:3.63E-4 ID:SGTC_888|Compound:0916-0002|FD-Score:5.22|P-value:1.10E-6 ID:SGTC_893|Compound:0958-0369|FD-Score:-5.48|P-value:2.32E-7 ID:SGTC_1131|Compound:3454-3128|FD-Score:5.58|P-value:2.06E-7 ID:SGTC_1161|Compound:k007-0610|FD-Score:-3.43|P-value:7.60E-4 ID:SGTC_1193|Compound:1488-0126|FD-Score:3.47|P-value:8.49E-4 ID:SGTC_1245|Compound:0418-0076|FD-Score:4.17|P-value:7.90E-5 ID:SGTC_1258|Compound:0671-0043|FD-Score:-3.61|P-value:4.27E-4 ID:SGTC_1522|Compound:st012842|FD-Score:-3.79|P-value:2.37E-4 ID:SGTC_1707|Compound:st032323|FD-Score:4.65|P-value:1.27E-5 ID:SGTC_1712|Compound:st032288|FD-Score:-4.92|P-value:3.00E-6 ID:SGTC_1718|Compound:roemerine|FD-Score:-6.42|P-value:1.83E-9 ID:SGTC_1836|Compound:st055642|FD-Score:-3.48|P-value:6.61E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:-4.15|P-value:6.62E-5 ID:SGTC_1916|Compound:st067606|FD-Score:5.55|P-value:2.35E-7 ID:SGTC_1930|Compound:st074634|FD-Score:-4.41|P-value:2.45E-5 ID:SGTC_2002|Compound:4023503|FD-Score:4.15|P-value:8.51E-5 ID:SGTC_2021|Compound:4019055|FD-Score:-3.41|P-value:8.23E-4 ID:SGTC_2509|Compound:methyl 7-deshydroxypyrogallin-4-carboxylate|FD-Score:-3.41|P-value:8.11E-4 ID:SGTC_2517|Compound:totarol|FD-Score:-3.68|P-value:3.41E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:-5.85|P-value:3.67E-8 ID:SGTC_2584|Compound:androsterone|FD-Score:4.20|P-value:7.17E-5 ID:SGTC_2591|Compound:skatole|FD-Score:3.52|P-value:7.29E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:4.86|P-value:5.21E-6 ID:SGTC_2772|Compound:vinpocetine|FD-Score:4.65|P-value:1.24E-5 ID:SGTC_2778|Compound:tioconazole|FD-Score:7.34|P-value:1.23E-11 ID:SGTC_2779|Compound:methotrexate|FD-Score:-4.75|P-value:6.18E-6 ID:SGTC_2784|Compound:7567673|FD-Score:-4.27|P-value:4.08E-5 ID:SGTC_2811|Compound:7967822|FD-Score:5.55|P-value:2.32E-7 ID:SGTC_2846|Compound:9008642|FD-Score:4.18|P-value:7.64E-5 ID:SGTC_2858|Compound:9025781|FD-Score:20.80|P-value:2.58E-80 ID:SGTC_2896|Compound:9052656|FD-Score:-5.11|P-value:1.29E-6 ID:SGTC_2942|Compound:9052777|FD-Score:3.94|P-value:1.81E-4 ID:SGTC_2947|Compound:9072639|FD-Score:5.05|P-value:2.36E-6 ID:SGTC_3063|Compound:1327-0034|FD-Score:5.70|P-value:1.17E-7 ID:SGTC_58|Compound:0957-0393|FD-Score:8.90|P-value:2.79E-16 ID:SGTC_108|Compound:0509-0257|FD-Score:4.56|P-value:1.81E-5 ID:SGTC_115|Compound:0527-0192|FD-Score:6.43|P-value:2.55E-9 ID:SGTC_143|Compound:4032-1194|FD-Score:6.32|P-value:4.74E-9 ID:SGTC_150|Compound:3970-0969|FD-Score:4.47|P-value:2.52E-5 ID:SGTC_156|Compound:3474-0145|FD-Score:3.79|P-value:3.00E-4 ID:SGTC_168|Compound:3970-0862|FD-Score:5.13|P-value:1.67E-6 ID:SGTC_207|Compound:4185-0010|FD-Score:3.73|P-value:3.74E-4 ID:SGTC_266|Compound:formosanin C|FD-Score:9.79|P-value:2.63E-19 ID:SGTC_299|Compound:1273-0059|FD-Score:-4.62|P-value:1.04E-5 ID:SGTC_310|Compound:1273-0058|FD-Score:-6.44|P-value:1.59E-9 ID:SGTC_312|Compound:4092-0653|FD-Score:3.80|P-value:2.94E-4 ID:SGTC_314|Compound:1671-0008|FD-Score:-3.98|P-value:1.21E-4 ID:SGTC_421|Compound:streptovitacin|FD-Score:6.11|P-value:1.44E-8 ID:SGTC_431|Compound:k089-0097|FD-Score:-5.93|P-value:2.45E-8 ID:SGTC_513|Compound:4245-1575|FD-Score:-4.55|P-value:1.40E-5 ID:SGTC_536|Compound:3852-0578|FD-Score:3.84|P-value:2.60E-4 ID:SGTC_664|Compound:1222-0054|FD-Score:4.24|P-value:6.24E-5 ID:SGTC_698|Compound:k064-0052|FD-Score:3.57|P-value:6.24E-4 ID:SGTC_885|Compound:0865-0106|FD-Score:3.74|P-value:3.63E-4 ID:SGTC_888|Compound:0916-0002|FD-Score:5.22|P-value:1.10E-6 ID:SGTC_893|Compound:0958-0369|FD-Score:-5.48|P-value:2.32E-7 ID:SGTC_1131|Compound:3454-3128|FD-Score:5.58|P-value:2.06E-7 ID:SGTC_1161|Compound:k007-0610|FD-Score:-3.43|P-value:7.60E-4 ID:SGTC_1193|Compound:1488-0126|FD-Score:3.47|P-value:8.49E-4 ID:SGTC_1245|Compound:0418-0076|FD-Score:4.17|P-value:7.90E-5 ID:SGTC_1258|Compound:0671-0043|FD-Score:-3.61|P-value:4.27E-4 ID:SGTC_1522|Compound:st012842|FD-Score:-3.79|P-value:2.37E-4 ID:SGTC_1707|Compound:st032323|FD-Score:4.65|P-value:1.27E-5 ID:SGTC_1712|Compound:st032288|FD-Score:-4.92|P-value:3.00E-6 ID:SGTC_1718|Compound:roemerine|FD-Score:-6.42|P-value:1.83E-9 ID:SGTC_1836|Compound:st055642|FD-Score:-3.48|P-value:6.61E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:-4.15|P-value:6.62E-5 ID:SGTC_1916|Compound:st067606|FD-Score:5.55|P-value:2.35E-7 ID:SGTC_1930|Compound:st074634|FD-Score:-4.41|P-value:2.45E-5 ID:SGTC_2002|Compound:4023503|FD-Score:4.15|P-value:8.51E-5 ID:SGTC_2021|Compound:4019055|FD-Score:-3.41|P-value:8.23E-4 ID:SGTC_2509|Compound:methyl 7-deshydroxypyrogallin-4-carboxylate|FD-Score:-3.41|P-value:8.11E-4 ID:SGTC_2517|Compound:totarol|FD-Score:-3.68|P-value:3.41E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:-5.85|P-value:3.67E-8 ID:SGTC_2584|Compound:androsterone|FD-Score:4.20|P-value:7.17E-5 ID:SGTC_2591|Compound:skatole|FD-Score:3.52|P-value:7.29E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:4.86|P-value:5.21E-6 ID:SGTC_2772|Compound:vinpocetine|FD-Score:4.65|P-value:1.24E-5 ID:SGTC_2778|Compound:tioconazole|FD-Score:7.34|P-value:1.23E-11 ID:SGTC_2779|Compound:methotrexate|FD-Score:-4.75|P-value:6.18E-6 ID:SGTC_2784|Compound:7567673|FD-Score:-4.27|P-value:4.08E-5 ID:SGTC_2811|Compound:7967822|FD-Score:5.55|P-value:2.32E-7 ID:SGTC_2846|Compound:9008642|FD-Score:4.18|P-value:7.64E-5 ID:SGTC_2858|Compound:9025781|FD-Score:20.80|P-value:2.58E-80 ID:SGTC_2896|Compound:9052656|FD-Score:-5.11|P-value:1.29E-6 ID:SGTC_2942|Compound:9052777|FD-Score:3.94|P-value:1.81E-4 ID:SGTC_2947|Compound:9072639|FD-Score:5.05|P-value:2.36E-6 ID:SGTC_3063|Compound:1327-0034|FD-Score:5.70|P-value:1.17E-7

Top fitness defect scores for YGL066W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2858 9025781 20.80 9.0 µM 2.58E-80 sphingolipid biosynthesis & PDR1 5 4.26E-96
2 SGTC_266 formosanin C 9.79 406.0 nM 2.63E-19 sphingolipid biosynthesis & PDR1 14 6.51E-23
3 SGTC_58 0957-0393 8.90 9.0 µM 2.79E-16 cell wall 102 2.78E-19
4 SGTC_2778 tioconazole 7.34 65.1 nM 1.23E-11 ergosterol depletion effects on membrane 23 1.04E-13
5 SGTC_115 0527-0192 6.43 112.0 µM 2.55E-9 13 6.25E-11
6 SGTC_143 4032-1194 6.32 15.7 µM 4.74E-9 18 1.32E-10
7 SGTC_421 streptovitacin 6.11 30.0 mM 1.44E-8 3 4.99E-10
8 SGTC_3063 1327-0034 5.70 4.0 µM 1.17E-7 24 6.13E-9
9 SGTC_1131 3454-3128 5.58 32.3 µM 2.06E-7 10 1.21E-8
10 SGTC_2811 7967822 5.55 32.5 µM 2.32E-7 6 1.39E-8
11 SGTC_1916 st067606 5.55 49.2 µM 2.35E-7 11 1.42E-8
12 SGTC_888 0916-0002 5.22 7.0 µM 1.10E-6 NEO1 26 8.98E-8
13 SGTC_168 3970-0862 5.13 530.5 µM 1.67E-6 68 1.48E-7
14 SGTC_2947 9072639 5.05 7.7 µM 2.36E-6 tubulin folding & SWR complex 16 2.25E-7
15 SGTC_2688 kanamycin b 4.86 96.7 µM 5.21E-6 calcium & mitochondrial duress 258 5.81E-7
16 SGTC_2772 vinpocetine 4.65 57.1 µM 1.24E-5 15 1.63E-6
17 SGTC_1707 st032323 4.65 6.3 µM 1.27E-5 DNA damage response 41 1.68E-6
18 SGTC_108 0509-0257 4.56 621.1 µM 1.81E-5 33 2.57E-6
19 SGTC_150 3970-0969 4.47 77.2 µM 2.52E-5 46 3.83E-6
20 SGTC_664 1222-0054 4.24 14.9 µM 6.24E-5 sphingolipid biosynthesis & PDR1 32 1.14E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.335 7.12E-89 YLR055C SPT8 hom Subunit of the SAGA transcriptional regulatory complex but not present in SAGA-like complex SLIK/SALSA, required for SAGA-mediated inhibition at some promoters
0.240 3.88E-45 YDR392W SPT3 hom Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters
0.224 2.30E-39 YML057W CMP2 hom Calcineurin A; one isoform (the other is CNA1) of the catalytic subunit of calcineurin, a Ca++/calmodulin-regulated protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is CNB1
0.217 6.30E-37 YDR391C_p YDR391C_p hom Putative protein of unknown function, possibly involved in zinc homeostasis; Bdf1p-dependent transcription induced by salt stress; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus
0.209 2.66E-34 YBL079W NUP170 hom Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC assembly and nucleocytoplasmic transport; both Nup170p and NUP157p are similar to human Nup155p; NUP170 has a paralog, NUP157, that arose from the whole genome duplication
0.207 6.11E-34 YPL153C RAD53 het Protein kinase, required for cell-cycle arrest in response to DNA damage; activated by trans autophosphorylation when interacting with hyperphosphorylated Rad9p; also interacts with ARS1 and plays a role in initiation of DNA replication
0.201 4.63E-32 YPL057C SUR1 hom Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Csh1p; SUR1 has a paralog, CSH1, that arose from the whole genome duplication
0.190 1.76E-28 YBL064C PRX1 hom Mitochondrial peroxiredoxin with thioredoxin peroxidase activity; has a role in reduction of hydroperoxides; reactivation requires Trr2p and glutathione; induced during respiratory growth and oxidative stress; phosphorylated; protein abundance increases in response to DNA replication stress
0.180 8.34E-26 YPL056C_p LCL1_p hom Putative protein of unknown function; deletion mutant is fluconazole resistant and has long chronological lifespan
0.172 1.11E-23 YNL229C URE2 hom Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion
0.172 1.47E-23 YDR072C IPT1 hom Inositolphosphotransferase, involved in synthesis of mannose-(inositol-P)2-ceramide (M(IP)2C), the most abundant sphingolipid; can mutate to resistance to the antifungals syringomycin E and DmAMP1 and to K. lactis zymocin
0.163 2.32E-21 YEL044W IES6 hom Protein that associates with the INO80 chromatin remodeling complex under low-salt conditions; human ortholog INO80C is a member of the human INO80 complex; implicated in DNA repair based on genetic interactions with RAD52 epistasis genes
0.157 6.80E-20 YGR058W PEF1 hom Penta-EF-hand protein required for polar bud growth and cell wall abscission; binds calcium and zinc with different affinity; localizes to bud site in G1, bud neck in G2; binds to Sec31p and modulates COPII coat assembly
0.156 1.01E-19 YGL253W HXK2 hom Hexokinase isoenzyme 2; catalyzes phosphorylation of glucose in the cytosol; predominant hexokinase during growth on glucose; functions in the nucleus to repress expression of HXK1 and GLK1 and to induce expression of its own gene; phosphorylation/dephosphorylation at serine-14 by protein kinase Snf1p and protein phosphatase Glc7p-Reg1p regulates nucleocytoplasmic shuttling of Hxk2p; HXK2 has a paralog, HXK1, that arose from the whole genome duplication
0.156 1.27E-19 YJR074W MOG1 hom Conserved nuclear protein that interacts with GTP-Gsp1p, which is a Ran homolog of the Ras GTPase family, and stimulates nucleotide release, involved in nuclear protein import, nucleotide release is inhibited by Yrb1p