HIPHOP chemogenomics database

YGL068W / MNP1

Protein associated with the mitochondrial nucleoid; putative mitochondrial ribosomal protein with similarity to E. coli L7/L12 ribosomal protein; required for normal respiratory growth

Zygosity: Heterozygous strain

fixedexpanded
Profile for YGL068W / MNP1

Click on Significant Values for Screen Details

Top fitness defect scores for YGL068W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value	Screen Clearance
1	SGTC_852	0418-0143	6.17	439.0 µM	3.64E-10	calcium & mitochondrial duress	3	3.36E-10	0.53
2	SGTC_124	0136-0217	5.08	7.1 µM	2.03E-7	mitochondrial stress	13	1.87E-7	0.02
3	SGTC_1785	bathophenanthroline	5.01	24.0 µM	2.88E-7	heme requiring	12	2.65E-7	0.05
4	SGTC_2013	4024628	4.77	200.0 µM	1.00E-6		7	9.24E-7	0.19
5	SGTC_1539	benzyladenosine	4.73	56.0 µM	1.21E-6		5	1.11E-6	0.69
6	SGTC_2418	pentamidine	4.71	31.7 µM	1.33E-6	mitochondrial processes	11	1.23E-6	0.03
7	SGTC_173	1273-0061	4.38	2.7 µM	6.37E-6	mitochondrial stress	20	5.88E-6	0.01
8	SGTC_141	0443-0274	4.34	32.3 µM	7.82E-6	mitochondrial response to ROS	3	7.22E-6	0.23
9	SGTC_3243	9134147	4.33	49.5 µM	7.98E-6		2	7.37E-6	0.18
10	SGTC_119	0121-0043	4.29	24.8 µM	9.61E-6	mitochondrial stress	15	8.87E-6	0.05
11	SGTC_348	k064-0012	3.99	10.7 µM	3.54E-5	60S ribosome export	4	3.28E-5	0.00
12	SGTC_2417	cerulenin	3.96	830.0 nM	4.00E-5		10	3.70E-5	5.27E-4
13	SGTC_2564	betulinic acid	3.88	100.0 µM	5.58E-5		8	5.17E-5	0.08
14	SGTC_1159	4092-0538	3.86	61.2 µM	6.02E-5		4	5.57E-5	0.02
15	SGTC_2408	sulbentine	3.86	18.4 µM	6.21E-5		36	5.75E-5	0.12
16	SGTC_674	0498-0004	3.78	451.0 µM	8.30E-5	heme requiring	23	7.69E-5	0.01
17	SGTC_1708	st033234	3.77	31.8 µM	8.72E-5		4	8.08E-5	0.08
18	SGTC_2640	pentamidine	3.76	10.4 µM	9.04E-5	mitochondrial processes	20	8.37E-5	0.03
19	SGTC_3062	9077494	3.71	49.5 µM	1.14E-4	mitochondrial response to ROS	10	1.06E-4	0.09
20	SGTC_116	0155-0182	3.67	31.8 µM	1.28E-4	mitochondrial response to ROS	5	1.19E-4	0.02

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.154	2.56E-19	YFL036W	RPO41	hom	Mitochondrial RNA polymerase; single subunit enzyme similar to those of T3 and T7 bacteriophages; requires a specificity subunit encoded by MTF1 for promoter recognition; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation
0.149	4.43E-18	YOR109W	INP53	hom	Polyphosphatidylinositol phosphatase, dephosphorylates multiple phosphatidylinositols; involved in trans Golgi network-to-early endosome pathway; hyperosmotic stress causes translocation to actin patches; contains Sac1 and 5-ptase domains
0.144	5.74E-17	YDL217C	TIM22	het	Essential core component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane; forms the channel through which proteins are imported
0.140	3.38E-16	YCR024C	SLM5	hom	Mitochondrial asparaginyl-tRNA synthetase
0.129	7.55E-14	YOR232W	MGE1	het	Mitochondrial matrix cochaperone, acts as a nucleotide release factor for Ssc1p in protein translocation and folding; also acts as cochaperone for Ssq1p in folding of Fe-S cluster proteins; homolog of E. coli GrpE
0.127	1.45E-13	YLR355C	ILV5	het	Bifunctional acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids
0.126	3.00E-13	YPL266W	DIM1	het	Essential 18S rRNA dimethylase (dimethyladenosine transferase), responsible for conserved m6(2)Am6(2)A dimethylation in 3'-terminal loop of 18S rRNA, part of 90S and 40S pre-particles in nucleolus, involved in pre-ribosomal RNA processing
0.123	8.80E-13	YPL170W	DAP1	hom	Heme-binding protein involved in regulation of cytochrome P450 protein Erg11p; damage response protein, related to mammalian membrane progesterone receptors; mutations lead to defects in telomeres, mitochondria, and sterol synthesis
0.121	1.72E-12	YNL268W	LYP1	hom	Lysine permease; one of three amino acid permeases (Alp1p, Can1p, Lyp1p) responsible for uptake of cationic amino acids
0.120	3.68E-12	YFL016C	MDJ1	hom	Co-chaperone that stimulates the ATPase activity of the HSP70 protein Ssc1p; involved in protein folding/refolding in the mitochodrial matrix; required for proteolysis of misfolded proteins; member of the HSP40 (DnaJ) family of chaperones
0.117	1.20E-11	YNR037C	RSM19	hom	Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S19 ribosomal protein
0.116	1.40E-11	YNL006W	LST8	het	Protein required for the transport of amino acid permease Gap1p from the Golgi to the cell surface; component of the TOR signaling pathway; associates with both Tor1p and Tor2p; contains a WD-repeat
0.113	4.52E-11	YGL064C	MRH4	hom	Mitochondrial ATP-dependent RNA helicase of the DEAD-box family, plays an essential role in mitochondrial function
0.113	6.15E-11	YBR179C	FZO1	hom	Mitofusin; integral membrane protein involved in mitochondrial outer membrane tethering and fusion; role in mitochondrial genome maintenance; efficient tethering and degradation of Fzo1p requires an intact N-terminal GTPase domain; targeted for destruction by the ubiquitin ligase SCF-Mdm30p and the cytosolic ubiquitin-proteasome system
0.112	7.48E-11	YKR085C	MRPL20	hom	Mitochondrial ribosomal protein of the large subunit