YGL070C / RPB9

RNA polymerase II subunit B12.6; contacts DNA; mutations affect transcription start site selection and fidelity of transcription

Zygosity: Homozygous strain
fixedexpanded
Profile for YGL070C / RPB9

Click on Significant Values for Screen Details ID:SGTC_57|Compound:miconazole|FD-Score:-5.25|P-value:1.62E-6 ID:SGTC_103|Compound:0844-0104|FD-Score:-3.85|P-value:3.11E-4 ID:SGTC_264|Compound:promethazine|FD-Score:5.41|P-value:1.08E-6 ID:SGTC_273|Compound:hydroxyurea|FD-Score:3.99|P-value:2.43E-4 ID:SGTC_479|Compound:n-linoleoylglycine|FD-Score:-4.43|P-value:4.24E-5 ID:SGTC_491|Compound:tetrandrine|FD-Score:-4.40|P-value:4.67E-5 ID:SGTC_744|Compound:1594-0068|FD-Score:3.83|P-value:4.16E-4 ID:SGTC_800|Compound:3448-7362|FD-Score:3.72|P-value:5.84E-4 ID:SGTC_1054|Compound:3429-0080|FD-Score:-3.96|P-value:2.20E-4 ID:SGTC_1060|Compound:3448-7350|FD-Score:3.58|P-value:8.99E-4 ID:SGTC_1117|Compound:retinoic acid|FD-Score:-4.67|P-value:1.73E-5 ID:SGTC_1263|Compound:0774-1208|FD-Score:-4.17|P-value:1.08E-4 ID:SGTC_1352|Compound:1496-0991|FD-Score:4.20|P-value:1.21E-4 ID:SGTC_1427|Compound:4048-4900|FD-Score:4.28|P-value:9.20E-5 ID:SGTC_1484|Compound:3473-2379|FD-Score:3.61|P-value:8.11E-4 ID:SGTC_1537|Compound:2',6'-dihydroxyflavanone|FD-Score:4.55|P-value:3.48E-5 ID:SGTC_1750|Compound:st081859|FD-Score:4.07|P-value:1.91E-4 ID:SGTC_2293|Compound:7624732|FD-Score:3.67|P-value:6.89E-4 ID:SGTC_2296|Compound:7971656|FD-Score:4.67|P-value:2.21E-5 ID:SGTC_2397|Compound:5161689|FD-Score:4.55|P-value:3.47E-5 ID:SGTC_2498|Compound:4'-Methoxychalcone|FD-Score:4.51|P-value:4.06E-5 ID:SGTC_2810|Compound:7959871|FD-Score:4.58|P-value:3.12E-5 ID:SGTC_2827|Compound:7993847|FD-Score:-3.50|P-value:9.32E-4 ID:SGTC_3018|Compound:9082602|FD-Score:-3.94|P-value:2.35E-4 ID:SGTC_3071|Compound:9117417|FD-Score:-4.67|P-value:1.74E-5 ID:SGTC_3076|Compound:9116187|FD-Score:-3.82|P-value:3.49E-4 ID:SGTC_3081|Compound:9117243|FD-Score:-4.23|P-value:8.53E-5 ID:SGTC_3192|Compound:9110362|FD-Score:4.01|P-value:2.31E-4 ID:SGTC_3201|Compound:9113999|FD-Score:4.72|P-value:1.79E-5 ID:SGTC_57|Compound:miconazole|FD-Score:-5.25|P-value:1.62E-6 ID:SGTC_103|Compound:0844-0104|FD-Score:-3.85|P-value:3.11E-4 ID:SGTC_264|Compound:promethazine|FD-Score:5.41|P-value:1.08E-6 ID:SGTC_273|Compound:hydroxyurea|FD-Score:3.99|P-value:2.43E-4 ID:SGTC_479|Compound:n-linoleoylglycine|FD-Score:-4.43|P-value:4.24E-5 ID:SGTC_491|Compound:tetrandrine|FD-Score:-4.40|P-value:4.67E-5 ID:SGTC_744|Compound:1594-0068|FD-Score:3.83|P-value:4.16E-4 ID:SGTC_800|Compound:3448-7362|FD-Score:3.72|P-value:5.84E-4 ID:SGTC_1054|Compound:3429-0080|FD-Score:-3.96|P-value:2.20E-4 ID:SGTC_1060|Compound:3448-7350|FD-Score:3.58|P-value:8.99E-4 ID:SGTC_1117|Compound:retinoic acid|FD-Score:-4.67|P-value:1.73E-5 ID:SGTC_1263|Compound:0774-1208|FD-Score:-4.17|P-value:1.08E-4 ID:SGTC_1352|Compound:1496-0991|FD-Score:4.20|P-value:1.21E-4 ID:SGTC_1427|Compound:4048-4900|FD-Score:4.28|P-value:9.20E-5 ID:SGTC_1484|Compound:3473-2379|FD-Score:3.61|P-value:8.11E-4 ID:SGTC_1537|Compound:2',6'-dihydroxyflavanone|FD-Score:4.55|P-value:3.48E-5 ID:SGTC_1750|Compound:st081859|FD-Score:4.07|P-value:1.91E-4 ID:SGTC_2293|Compound:7624732|FD-Score:3.67|P-value:6.89E-4 ID:SGTC_2296|Compound:7971656|FD-Score:4.67|P-value:2.21E-5 ID:SGTC_2397|Compound:5161689|FD-Score:4.55|P-value:3.47E-5 ID:SGTC_2498|Compound:4'-Methoxychalcone|FD-Score:4.51|P-value:4.06E-5 ID:SGTC_2810|Compound:7959871|FD-Score:4.58|P-value:3.12E-5 ID:SGTC_2827|Compound:7993847|FD-Score:-3.50|P-value:9.32E-4 ID:SGTC_3018|Compound:9082602|FD-Score:-3.94|P-value:2.35E-4 ID:SGTC_3071|Compound:9117417|FD-Score:-4.67|P-value:1.74E-5 ID:SGTC_3076|Compound:9116187|FD-Score:-3.82|P-value:3.49E-4 ID:SGTC_3081|Compound:9117243|FD-Score:-4.23|P-value:8.53E-5 ID:SGTC_3192|Compound:9110362|FD-Score:4.01|P-value:2.31E-4 ID:SGTC_3201|Compound:9113999|FD-Score:4.72|P-value:1.79E-5

Top fitness defect scores for YGL070C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_264 promethazine 5.41 12.5 mM 1.08E-6 DNA intercalators 25 3.13E-8
2 SGTC_3201 9113999 4.72 49.5 µM 1.79E-5 19 1.15E-6
3 SGTC_2296 7971656 4.67 126.5 µM 2.21E-5 27 1.51E-6
4 SGTC_2810 7959871 4.58 45.5 µM 3.12E-5 copper-dependent oxidative stress 28 2.34E-6
5 SGTC_2397 5161689 4.55 200.0 µM 3.47E-5 19 2.69E-6
6 SGTC_1537 2',6'-dihydroxyflavanone 4.55 78.0 µM 3.48E-5 7 2.70E-6
7 SGTC_2498 4'-Methoxychalcone 4.51 54.6 µM 4.06E-5 RPP1 & pyrimidine depletion 22 3.28E-6
8 SGTC_1427 4048-4900 4.28 550.0 nM 9.20E-5 21 9.39E-6
9 SGTC_1352 1496-0991 4.20 99.0 µM 1.21E-4 tubulin folding & SWR complex 28 1.33E-5
10 SGTC_1750 st081859 4.07 2.4 µM 1.91E-4 endomembrane recycling 178 2.39E-5
11 SGTC_3192 9110362 4.01 49.5 µM 2.31E-4 mitochondrial processes 12 3.05E-5
12 SGTC_273 hydroxyurea 3.99 18.1 mM 2.43E-4 heme biosynthesis & mitochondrial translocase 36 3.25E-5
13 SGTC_744 1594-0068 3.83 283.0 µM 4.16E-4 amide catabolism 31 6.49E-5
14 SGTC_800 3448-7362 3.72 155.0 µM 5.84E-4 translation 181 1.00E-4
15 SGTC_2293 7624732 3.67 200.0 µM 6.89E-4 33 1.24E-4
16 SGTC_1484 3473-2379 3.61 286.0 µM 8.11E-4 29 1.52E-4
17 SGTC_1060 3448-7350 3.58 82.0 µM 8.99E-4 Golgi 43 1.74E-4
18 SGTC_2399 5884133 3.50 200.0 µM 0.00112 cell wall signaling 94 2.31E-4
19 SGTC_2282 7944268 3.42 200.0 µM 0.00141 RPP1 & pyrimidine depletion 56 3.07E-4
20 SGTC_1683 st010134 3.38 710.0 nM 0.00159 53 3.61E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.104 1.83E-9 YMR126C_p DLT1_p hom Protein of unknown function, mutant sensitive to 6-azauracil (6AU) and mycophenolic acid (MPA)
0.101 5.01E-9 YMR166C_p YMR166C_p hom Predicted transporter of the mitochondrial inner membrane; has similarity to human mitochondrial ATP-Mg/Pi carriers; YMR166C is not an essential gene
0.098 1.86E-8 YHR062C RPP1 het Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
0.094 4.96E-8 YMR289W ABZ2 hom Aminodeoxychorismate lyase (4-amino-4-deoxychorismate lyase), catalyzes the third step in para-aminobenzoic acid biosynthesis; involved in folic acid biosynthesis
0.092 9.27E-8 YJL027C_p YJL027C_p hom Putative protein of unknown function
0.092 9.31E-8 YPR029C APL4 hom Gamma-adaptin, large subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in vesicle mediated transport
0.092 1.09E-7 YHL039W EFM1 hom Lysine methyltransferase involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.090 1.74E-7 YMR168C CEP3 het Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain
0.088 2.98E-7 YGL095C VPS45 hom Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment
0.086 5.75E-7 YDR113C PDS1 het Securin, inhibits anaphase by binding separin Esp1p; blocks cyclin destruction and mitotic exit, essential for meiotic progression and mitotic cell cycle arrest; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation
0.085 8.50E-7 YJL117W PHO86 hom Endoplasmic reticulum (ER) resident protein; required for ER exit of the high-affinity phosphate transporter Pho84p, specifically required for packaging of Pho84p into COPII vesicles; protein abundance increases in response to DNA replication stress
0.083 1.70E-6 YDR465C RMT2 hom Arginine N5 methyltransferase; methylates ribosomal protein Rpl12 (L12) on Arg67; relative distribution to the nucleus increases upon DNA replication stress
0.082 1.89E-6 YOR133W EFT1 hom Elongation factor 2 (EF-2), also encoded by EFT2; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT1 has a paralog, EFT2, that arose from the whole genome duplication
0.081 3.13E-6 YHR172W SPC97 het Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque
0.080 3.57E-6 YGR065C VHT1 het High-affinity plasma membrane H+-biotin (vitamin H) symporter; mutation results in fatty acid auxotrophy; 12 transmembrane domain containing major facilitator subfamily member; mRNA levels negatively regulated by iron deprivation and biotin