YGL073W / HSF1

Trimeric heat shock transcription factor, activates multiple genes in response to stresses that include hyperthermia; recognizes variable heat shock elements (HSEs) consisting of inverted NGAAN repeats; posttranslationally regulated

Zygosity: Heterozygous strain
fixedexpanded
Profile for YGL073W / HSF1

Click on Significant Values for Screen Details ID:SGTC_140|Compound:0062-0056|FD-Score:-3.88|P-value:7.48E-6 ID:SGTC_165|Compound:k038-0023|FD-Score:6.67|P-value:3.35E-14 ID:SGTC_166|Compound:k072-0058|FD-Score:-6.68|P-value:4.32E-14 ID:SGTC_167|Compound:3672-0051|FD-Score:-4.04|P-value:3.40E-6 ID:SGTC_168|Compound:3970-0862|FD-Score:-5.00|P-value:1.21E-8 ID:SGTC_264|Compound:promethazine|FD-Score:-3.39|P-value:8.07E-5 ID:SGTC_276|Compound:colchicine|FD-Score:2.77|P-value:8.72E-4 ID:SGTC_315|Compound:k915-0449|FD-Score:3.26|P-value:1.17E-4 ID:SGTC_329|Compound:k048-0121|FD-Score:3.91|P-value:5.44E-6 ID:SGTC_430|Compound:0958-0115|FD-Score:2.81|P-value:7.55E-4 ID:SGTC_483|Compound:fpl-64176|FD-Score:3.05|P-value:2.92E-4 ID:SGTC_513|Compound:4245-1575|FD-Score:8.24|P-value:1.05E-20 ID:SGTC_514|Compound:4466-0038|FD-Score:6.77|P-value:1.42E-14 ID:SGTC_515|Compound:4092-1064|FD-Score:3.85|P-value:7.12E-6 ID:SGTC_541|Compound:4513-1321|FD-Score:4.56|P-value:1.42E-7 ID:SGTC_543|Compound:3844-1095|FD-Score:3.27|P-value:1.13E-4 ID:SGTC_717|Compound:1532-0246|FD-Score:-2.84|P-value:7.86E-4 ID:SGTC_718|Compound:1130-0073|FD-Score:-2.86|P-value:7.28E-4 ID:SGTC_843|Compound:0310-0051|FD-Score:3.11|P-value:2.30E-4 ID:SGTC_1130|Compound:3454-2858|FD-Score:3.23|P-value:1.38E-4 ID:SGTC_1611|Compound:st001856|FD-Score:3.28|P-value:1.08E-4 ID:SGTC_1681|Compound:tripelennamine|FD-Score:3.07|P-value:2.64E-4 ID:SGTC_1845|Compound:st056753|FD-Score:-3.24|P-value:1.55E-4 ID:SGTC_1977|Compound:st070720|FD-Score:-4.27|P-value:9.63E-7 ID:SGTC_1981|Compound:st071826|FD-Score:-2.78|P-value:9.90E-4 ID:SGTC_1987|Compound:st072290|FD-Score:2.79|P-value:8.08E-4 ID:SGTC_1996|Compound:st072960|FD-Score:6.16|P-value:2.11E-12 ID:SGTC_2248|Compound:tofa|FD-Score:4.50|P-value:2.07E-7 ID:SGTC_2302|Compound:7771753|FD-Score:-3.55|P-value:3.76E-5 ID:SGTC_2322|Compound:7413319|FD-Score:3.32|P-value:9.15E-5 ID:SGTC_2340|Compound:7987427|FD-Score:3.04|P-value:3.06E-4 ID:SGTC_2402|Compound:5553605|FD-Score:2.79|P-value:8.30E-4 ID:SGTC_2424|Compound:5537928|FD-Score:-3.06|P-value:3.28E-4 ID:SGTC_2483|Compound:5729050|FD-Score:3.31|P-value:9.49E-5 ID:SGTC_2565|Compound:isoliquiritigenin|FD-Score:-2.96|P-value:4.91E-4 ID:SGTC_2584|Compound:androsterone|FD-Score:2.78|P-value:8.44E-4 ID:SGTC_2639|Compound:lactacystin|FD-Score:6.24|P-value:1.11E-12 ID:SGTC_2725|Compound:haloperidol|FD-Score:-3.26|P-value:1.44E-4 ID:SGTC_2728|Compound:chlorpromazine|FD-Score:-2.81|P-value:8.75E-4 ID:SGTC_2747|Compound:doxorubicin|FD-Score:3.41|P-value:6.17E-5 ID:SGTC_2827|Compound:7993847|FD-Score:2.88|P-value:5.73E-4 ID:SGTC_2846|Compound:9008642|FD-Score:3.19|P-value:1.58E-4 ID:SGTC_2896|Compound:9052656|FD-Score:2.82|P-value:7.21E-4 ID:SGTC_2952|Compound:9073731|FD-Score:-6.09|P-value:5.04E-12 ID:SGTC_2965|Compound:9082772|FD-Score:-3.50|P-value:4.94E-5 ID:SGTC_3009|Compound:9080819|FD-Score:4.37|P-value:4.32E-7 ID:SGTC_3067|Compound:9117431|FD-Score:2.92|P-value:4.95E-4 ID:SGTC_3142|Compound:9086498|FD-Score:3.13|P-value:2.06E-4 ID:SGTC_3194|Compound:9111903|FD-Score:-3.06|P-value:3.28E-4 ID:SGTC_3197|Compound:9113331|FD-Score:-3.92|P-value:6.22E-6 ID:SGTC_3200|Compound:9114385|FD-Score:-4.76|P-value:5.55E-8 ID:SGTC_3245|Compound:9134821|FD-Score:3.17|P-value:1.74E-4 ID:SGTC_3248|Compound:9135562|FD-Score:2.77|P-value:8.93E-4 ID:SGTC_3253|Compound:9135987|FD-Score:-3.22|P-value:1.68E-4 ID:SGTC_3323|Compound:9138564|FD-Score:-3.22|P-value:1.66E-4 ID:SGTC_3335|Compound:9143315|FD-Score:-2.91|P-value:6.13E-4 ID:SGTC_140|Compound:0062-0056|FD-Score:-3.88|P-value:7.48E-6 ID:SGTC_165|Compound:k038-0023|FD-Score:6.67|P-value:3.35E-14 ID:SGTC_166|Compound:k072-0058|FD-Score:-6.68|P-value:4.32E-14 ID:SGTC_167|Compound:3672-0051|FD-Score:-4.04|P-value:3.40E-6 ID:SGTC_168|Compound:3970-0862|FD-Score:-5.00|P-value:1.21E-8 ID:SGTC_264|Compound:promethazine|FD-Score:-3.39|P-value:8.07E-5 ID:SGTC_276|Compound:colchicine|FD-Score:2.77|P-value:8.72E-4 ID:SGTC_315|Compound:k915-0449|FD-Score:3.26|P-value:1.17E-4 ID:SGTC_329|Compound:k048-0121|FD-Score:3.91|P-value:5.44E-6 ID:SGTC_430|Compound:0958-0115|FD-Score:2.81|P-value:7.55E-4 ID:SGTC_483|Compound:fpl-64176|FD-Score:3.05|P-value:2.92E-4 ID:SGTC_513|Compound:4245-1575|FD-Score:8.24|P-value:1.05E-20 ID:SGTC_514|Compound:4466-0038|FD-Score:6.77|P-value:1.42E-14 ID:SGTC_515|Compound:4092-1064|FD-Score:3.85|P-value:7.12E-6 ID:SGTC_541|Compound:4513-1321|FD-Score:4.56|P-value:1.42E-7 ID:SGTC_543|Compound:3844-1095|FD-Score:3.27|P-value:1.13E-4 ID:SGTC_717|Compound:1532-0246|FD-Score:-2.84|P-value:7.86E-4 ID:SGTC_718|Compound:1130-0073|FD-Score:-2.86|P-value:7.28E-4 ID:SGTC_843|Compound:0310-0051|FD-Score:3.11|P-value:2.30E-4 ID:SGTC_1130|Compound:3454-2858|FD-Score:3.23|P-value:1.38E-4 ID:SGTC_1611|Compound:st001856|FD-Score:3.28|P-value:1.08E-4 ID:SGTC_1681|Compound:tripelennamine|FD-Score:3.07|P-value:2.64E-4 ID:SGTC_1845|Compound:st056753|FD-Score:-3.24|P-value:1.55E-4 ID:SGTC_1977|Compound:st070720|FD-Score:-4.27|P-value:9.63E-7 ID:SGTC_1981|Compound:st071826|FD-Score:-2.78|P-value:9.90E-4 ID:SGTC_1987|Compound:st072290|FD-Score:2.79|P-value:8.08E-4 ID:SGTC_1996|Compound:st072960|FD-Score:6.16|P-value:2.11E-12 ID:SGTC_2248|Compound:tofa|FD-Score:4.50|P-value:2.07E-7 ID:SGTC_2302|Compound:7771753|FD-Score:-3.55|P-value:3.76E-5 ID:SGTC_2322|Compound:7413319|FD-Score:3.32|P-value:9.15E-5 ID:SGTC_2340|Compound:7987427|FD-Score:3.04|P-value:3.06E-4 ID:SGTC_2402|Compound:5553605|FD-Score:2.79|P-value:8.30E-4 ID:SGTC_2424|Compound:5537928|FD-Score:-3.06|P-value:3.28E-4 ID:SGTC_2483|Compound:5729050|FD-Score:3.31|P-value:9.49E-5 ID:SGTC_2565|Compound:isoliquiritigenin|FD-Score:-2.96|P-value:4.91E-4 ID:SGTC_2584|Compound:androsterone|FD-Score:2.78|P-value:8.44E-4 ID:SGTC_2639|Compound:lactacystin|FD-Score:6.24|P-value:1.11E-12 ID:SGTC_2725|Compound:haloperidol|FD-Score:-3.26|P-value:1.44E-4 ID:SGTC_2728|Compound:chlorpromazine|FD-Score:-2.81|P-value:8.75E-4 ID:SGTC_2747|Compound:doxorubicin|FD-Score:3.41|P-value:6.17E-5 ID:SGTC_2827|Compound:7993847|FD-Score:2.88|P-value:5.73E-4 ID:SGTC_2846|Compound:9008642|FD-Score:3.19|P-value:1.58E-4 ID:SGTC_2896|Compound:9052656|FD-Score:2.82|P-value:7.21E-4 ID:SGTC_2952|Compound:9073731|FD-Score:-6.09|P-value:5.04E-12 ID:SGTC_2965|Compound:9082772|FD-Score:-3.50|P-value:4.94E-5 ID:SGTC_3009|Compound:9080819|FD-Score:4.37|P-value:4.32E-7 ID:SGTC_3067|Compound:9117431|FD-Score:2.92|P-value:4.95E-4 ID:SGTC_3142|Compound:9086498|FD-Score:3.13|P-value:2.06E-4 ID:SGTC_3194|Compound:9111903|FD-Score:-3.06|P-value:3.28E-4 ID:SGTC_3197|Compound:9113331|FD-Score:-3.92|P-value:6.22E-6 ID:SGTC_3200|Compound:9114385|FD-Score:-4.76|P-value:5.55E-8 ID:SGTC_3245|Compound:9134821|FD-Score:3.17|P-value:1.74E-4 ID:SGTC_3248|Compound:9135562|FD-Score:2.77|P-value:8.93E-4 ID:SGTC_3253|Compound:9135987|FD-Score:-3.22|P-value:1.68E-4 ID:SGTC_3323|Compound:9138564|FD-Score:-3.22|P-value:1.66E-4 ID:SGTC_3335|Compound:9143315|FD-Score:-2.91|P-value:6.13E-4

Top fitness defect scores for YGL073W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_513 4245-1575 8.24 41.7 µM 1.05E-20 unfolded protein response 5 8.56E-17 0.73
2 SGTC_514 4466-0038 6.77 2.6 nM 1.42E-14 cell wall 10 6.56E-12 0.16
3 SGTC_165 k038-0023 6.67 64.4 µM 3.35E-14 ubiquinone biosynthesis & proteasome 6 1.30E-11 0.27
4 SGTC_2639 lactacystin 6.24 53.0 µM 1.11E-12 2 2.13E-10 0.65
5 SGTC_1996 st072960 6.16 66.8 µM 2.11E-12 1 3.55E-10 1.12
6 SGTC_541 4513-1321 4.56 94.0 µM 1.42E-7 6 2.54E-6 0.42
7 SGTC_2248 tofa 4.50 1.1 µM 2.07E-7 sphingolipid biosynthesis & PDR1 23 3.44E-6 0.08
8 SGTC_3009 9080819 4.37 71.4 µM 4.32E-7 5 6.20E-6 0.05
9 SGTC_329 k048-0121 3.91 21.3 µM 5.44E-6 RSC & ERG11 13 4.71E-5 0.33
10 SGTC_515 4092-1064 3.85 102.0 µM 7.12E-6 plasma membrane duress 20 5.84E-5 0.10
11 SGTC_2747 doxorubicin 3.41 7.6 µM 6.17E-5 anthracycline transcription coupled DNA repair 9 3.30E-4 0.10
12 SGTC_2322 7413319 3.32 38.2 µM 9.15E-5 ERAD & cell cycle 37 4.53E-4 0.33
13 SGTC_2483 5729050 3.31 27.2 µM 9.49E-5 15 4.66E-4 0.06
14 SGTC_1611 st001856 3.28 99.4 µM 1.08E-4 18 5.18E-4 0.00
15 SGTC_543 3844-1095 3.27 191.0 µM 1.13E-4 ERG2 14 5.35E-4 0.22
16 SGTC_315 k915-0449 3.26 187.0 µM 1.17E-4 12 5.53E-4 0.03
17 SGTC_1130 3454-2858 3.23 15.9 µM 1.38E-4 NEO1 20 6.28E-4 0.08
18 SGTC_2846 9008642 3.19 19.5 µM 1.58E-4 15 7.00E-4 0.18
19 SGTC_3245 9134821 3.17 49.5 µM 1.74E-4 9 7.59E-4 0.02
20 SGTC_3142 9086498 3.13 49.5 µM 2.06E-4 iron homeostasis 62 8.68E-4 0.04

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.174 2.89E-24 YOR023C AHC1 hom Subunit of the Ada histone acetyltransferase complex, required for structural integrity of the complex
0.157 5.28E-20 YJR117W STE24 hom Highly conserved zinc metalloprotease that functions in two steps of a-factor maturation, C-terminal CAAX proteolysis and the first step of N-terminal proteolytic processing; contains multiple transmembrane spans
0.149 3.88E-18 YFL013W-A_d YFL013W-A_d hom Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps IES1/YFL013C; identified by gene-trapping, microarray expression analysis, and genome-wide homology searching
0.136 2.37E-15 YBL085W BOI1 hom Protein implicated in polar growth; functionally redundant with Boi2p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; relocalizes from bud neck to cytoplasm upon DNA replication stress; BOI1 has a paralog, BOI2, that arose from the whole genome duplication
0.133 1.04E-14 YDR121W DPB4 hom Subunit of DNA pol epsilon and of ISW2 chromatin accessibility complex; involved in both chromosomal DNA replication and inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; interacts with extranucleosomal DNA and acts as anchor point for ISW2 complex that retains its position on DNA during nucleosome mobilization
0.132 1.84E-14 YGL074C_d YGL074C_d het Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential HSF1 gene encoding heat shock transcription factor
0.127 1.32E-13 YGL112C TAF6 het Subunit (60 kDa) of TFIID and SAGA complexes, involved in transcription initiation of RNA polymerase II and in chromatin modification, similar to histone H4
0.118 7.64E-12 YMR121C RPL15B hom Ribosomal 60S subunit protein L15B; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15B has a paralog, RPL15A, that arose from the whole genome duplication; relocalizes from nucleus to nucleolus upon DNA replication stress
0.115 2.14E-11 YOL003C PFA4 hom Palmitoyltransferase with autoacylation activity, required for palmitoylation of amino acid permeases containing a C-terminal Phe-Trp-Cys site; required for modification of Chs3p; member of the DHHC family of putative palmitoyltransferases
0.112 6.62E-11 YPR061C JID1 hom Probable Hsp40p co-chaperone, has a DnaJ-like domain and appears to be involved in ER-associated degradation of misfolded proteins containing a tightly folded cytoplasmic domain; inhibits replication of Brome mosaic virus in S. cerevisiae
0.112 7.80E-11 YPR012W_d YPR012W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPR012W is not an essential gene
0.111 1.31E-10 YLR028C ADE16 hom Enzyme of 'de novo' purine biosynthesis; contains both 5-aminoimidazole-4-carboxamide ribonucleotide transformylase and inosine monophosphate cyclohydrolase activities; ADE16 has a paralog, ADE17, that arose from the whole genome duplication; ade16 ade17 mutants require adenine and histidine
0.110 2.00E-10 YKL213C DOA1 hom WD repeat protein required for ubiquitin-mediated protein degradation; forms a complex with Cdc48p; plays a role in controlling cellular ubiquitin concentration; also promotes efficient NHEJ in postdiauxic/stationary phase; protein increases in abundance and relocalizes from nucleus to nuclear periphery upon DNA replication stress
0.107 4.40E-10 YDL084W SUB2 het Component of the TREX complex required for nuclear mRNA export; member of the DEAD-box RNA helicase superfamily and is involved in early and late steps of spliceosome assembly; homolog of the human splicing factor hUAP56; relocalizes from nucleus to cytoplasm upon DNA replication stress
0.107 5.31E-10 YEL001C IRC22 hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion localizes to the ER; YEL001C is non-essential; null mutant displays increased levels of spontaneous Rad52p foci