YGL078C / DBP3

RNA-Dependent ATPase, member of DExD/H-box family; involved in cleavage of site A3 within the ITS1 spacer during rRNA processing; not essential for growth, but deletion causes severe slow-growth phenotype

Zygosity: Homozygous strain
fixedexpanded
Profile for YGL078C / DBP3

Click on Significant Values for Screen Details ID:SGTC_87|Compound:0100-0052|FD-Score:3.87|P-value:1.28E-4 ID:SGTC_118|Compound:0083-0097|FD-Score:4.19|P-value:3.67E-5 ID:SGTC_207|Compound:4185-0010|FD-Score:-3.32|P-value:8.89E-4 ID:SGTC_344|Compound:k064-0035|FD-Score:3.35|P-value:7.72E-4 ID:SGTC_348|Compound:k064-0012|FD-Score:5.93|P-value:1.01E-8 ID:SGTC_395|Compound:anisomycin|FD-Score:3.67|P-value:2.59E-4 ID:SGTC_439|Compound:amiodarone|FD-Score:-3.63|P-value:3.19E-4 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:-3.53|P-value:4.46E-4 ID:SGTC_459|Compound:nigericin|FD-Score:-3.91|P-value:1.15E-4 ID:SGTC_496|Compound:calpeptin|FD-Score:3.82|P-value:1.49E-4 ID:SGTC_537|Compound:1334-0398|FD-Score:3.68|P-value:2.53E-4 ID:SGTC_735|Compound:3076-0345|FD-Score:4.37|P-value:1.81E-5 ID:SGTC_750|Compound:4073-0104|FD-Score:3.95|P-value:9.39E-5 ID:SGTC_1131|Compound:3454-3128|FD-Score:4.01|P-value:7.39E-5 ID:SGTC_1136|Compound:pergolide|FD-Score:3.68|P-value:2.50E-4 ID:SGTC_1438|Compound:4092-1045|FD-Score:3.56|P-value:3.76E-4 ID:SGTC_1526|Compound:st051518|FD-Score:3.37|P-value:7.05E-4 ID:SGTC_1578|Compound:josamycin|FD-Score:5.25|P-value:3.45E-7 ID:SGTC_1605|Compound:st000082|FD-Score:3.34|P-value:7.83E-4 ID:SGTC_1839|Compound:st053978|FD-Score:4.40|P-value:1.55E-5 ID:SGTC_1872|Compound:amphotericin b|FD-Score:-3.59|P-value:3.68E-4 ID:SGTC_1876|Compound:myriocin|FD-Score:-3.84|P-value:1.48E-4 ID:SGTC_2265|Compound:7929201|FD-Score:4.00|P-value:7.63E-5 ID:SGTC_2419|Compound:paclitaxel|FD-Score:4.01|P-value:7.49E-5 ID:SGTC_2430|Compound:5571206|FD-Score:-3.46|P-value:5.59E-4 ID:SGTC_2435|Compound:5706476|FD-Score:-3.51|P-value:4.79E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:3.31|P-value:8.58E-4 ID:SGTC_2815|Compound:7968870|FD-Score:3.36|P-value:7.48E-4 ID:SGTC_2842|Compound:9000675|FD-Score:3.81|P-value:1.58E-4 ID:SGTC_2861|Compound:9024595|FD-Score:-3.78|P-value:1.86E-4 ID:SGTC_2863|Compound:9028737|FD-Score:-4.23|P-value:3.38E-5 ID:SGTC_2879|Compound:9041815|FD-Score:-4.24|P-value:3.34E-5 ID:SGTC_3043|Compound:9092303|FD-Score:3.31|P-value:8.56E-4 ID:SGTC_3066|Compound:9117245|FD-Score:4.37|P-value:1.78E-5 ID:SGTC_3191|Compound:9110352|FD-Score:4.15|P-value:4.33E-5 ID:SGTC_3243|Compound:9134147|FD-Score:3.65|P-value:2.80E-4 ID:SGTC_3258|Compound:9138477|FD-Score:3.76|P-value:1.85E-4 ID:SGTC_87|Compound:0100-0052|FD-Score:3.87|P-value:1.28E-4 ID:SGTC_118|Compound:0083-0097|FD-Score:4.19|P-value:3.67E-5 ID:SGTC_207|Compound:4185-0010|FD-Score:-3.32|P-value:8.89E-4 ID:SGTC_344|Compound:k064-0035|FD-Score:3.35|P-value:7.72E-4 ID:SGTC_348|Compound:k064-0012|FD-Score:5.93|P-value:1.01E-8 ID:SGTC_395|Compound:anisomycin|FD-Score:3.67|P-value:2.59E-4 ID:SGTC_439|Compound:amiodarone|FD-Score:-3.63|P-value:3.19E-4 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:-3.53|P-value:4.46E-4 ID:SGTC_459|Compound:nigericin|FD-Score:-3.91|P-value:1.15E-4 ID:SGTC_496|Compound:calpeptin|FD-Score:3.82|P-value:1.49E-4 ID:SGTC_537|Compound:1334-0398|FD-Score:3.68|P-value:2.53E-4 ID:SGTC_735|Compound:3076-0345|FD-Score:4.37|P-value:1.81E-5 ID:SGTC_750|Compound:4073-0104|FD-Score:3.95|P-value:9.39E-5 ID:SGTC_1131|Compound:3454-3128|FD-Score:4.01|P-value:7.39E-5 ID:SGTC_1136|Compound:pergolide|FD-Score:3.68|P-value:2.50E-4 ID:SGTC_1438|Compound:4092-1045|FD-Score:3.56|P-value:3.76E-4 ID:SGTC_1526|Compound:st051518|FD-Score:3.37|P-value:7.05E-4 ID:SGTC_1578|Compound:josamycin|FD-Score:5.25|P-value:3.45E-7 ID:SGTC_1605|Compound:st000082|FD-Score:3.34|P-value:7.83E-4 ID:SGTC_1839|Compound:st053978|FD-Score:4.40|P-value:1.55E-5 ID:SGTC_1872|Compound:amphotericin b|FD-Score:-3.59|P-value:3.68E-4 ID:SGTC_1876|Compound:myriocin|FD-Score:-3.84|P-value:1.48E-4 ID:SGTC_2265|Compound:7929201|FD-Score:4.00|P-value:7.63E-5 ID:SGTC_2419|Compound:paclitaxel|FD-Score:4.01|P-value:7.49E-5 ID:SGTC_2430|Compound:5571206|FD-Score:-3.46|P-value:5.59E-4 ID:SGTC_2435|Compound:5706476|FD-Score:-3.51|P-value:4.79E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:3.31|P-value:8.58E-4 ID:SGTC_2815|Compound:7968870|FD-Score:3.36|P-value:7.48E-4 ID:SGTC_2842|Compound:9000675|FD-Score:3.81|P-value:1.58E-4 ID:SGTC_2861|Compound:9024595|FD-Score:-3.78|P-value:1.86E-4 ID:SGTC_2863|Compound:9028737|FD-Score:-4.23|P-value:3.38E-5 ID:SGTC_2879|Compound:9041815|FD-Score:-4.24|P-value:3.34E-5 ID:SGTC_3043|Compound:9092303|FD-Score:3.31|P-value:8.56E-4 ID:SGTC_3066|Compound:9117245|FD-Score:4.37|P-value:1.78E-5 ID:SGTC_3191|Compound:9110352|FD-Score:4.15|P-value:4.33E-5 ID:SGTC_3243|Compound:9134147|FD-Score:3.65|P-value:2.80E-4 ID:SGTC_3258|Compound:9138477|FD-Score:3.76|P-value:1.85E-4

Top fitness defect scores for YGL078C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_348 k064-0012 5.93 10.7 µM 1.01E-8 60S ribosome export 5 1.47E-9
2 SGTC_1578 josamycin 5.25 24.2 µM 3.45E-7 4 7.58E-8
3 SGTC_1839 st053978 4.40 48.0 µM 1.55E-5 10 5.29E-6
4 SGTC_3066 9117245 4.37 49.5 µM 1.78E-5 9 6.17E-6
5 SGTC_735 3076-0345 4.37 636.0 µM 1.81E-5 20 6.30E-6
6 SGTC_118 0083-0097 4.19 135.3 µM 3.67E-5 translation 109 1.38E-5
7 SGTC_3191 9110352 4.15 49.5 µM 4.33E-5 7 1.66E-5
8 SGTC_1131 3454-3128 4.01 32.3 µM 7.39E-5 44 3.02E-5
9 SGTC_2419 paclitaxel 4.01 166.5 µM 7.49E-5 39 3.06E-5
10 SGTC_2265 7929201 4.00 200.0 µM 7.63E-5 20 3.12E-5
11 SGTC_750 4073-0104 3.95 132.0 µM 9.39E-5 13 3.94E-5
12 SGTC_87 0100-0052 3.87 35.5 µM 1.28E-4 115 5.55E-5
13 SGTC_496 calpeptin 3.82 138.0 µM 1.49E-4 19 6.59E-5
14 SGTC_2842 9000675 3.81 19.5 µM 1.58E-4 24 7.04E-5
15 SGTC_3258 9138477 3.76 49.5 µM 1.85E-4 8 8.38E-5
16 SGTC_1136 pergolide 3.68 1.1 µM 2.50E-4 49 1.17E-4
17 SGTC_537 1334-0398 3.68 209.0 µM 2.53E-4 34 1.19E-4
18 SGTC_395 anisomycin 3.67 6.2 µM 2.59E-4 12 1.22E-4
19 SGTC_3243 9134147 3.65 49.5 µM 2.80E-4 5 1.33E-4
20 SGTC_1438 4092-1045 3.56 4.0 µM 3.76E-4 13 1.84E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.139 6.88E-16 YLR185W RPL37A hom Ribosomal 60S subunit protein L37A; homologous to mammalian ribosomal protein L37, no bacterial homolog; RPL37A has a paralog, RPL37B, that arose from the whole genome duplication
0.121 2.03E-12 YNL043C_d YNL043C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YIP3/YNL044W
0.115 2.19E-11 YIR026C YVH1 hom Protein phosphatase involved in vegetative growth at low temperatures, sporulation, and glycogen accumulation; mutants are defective in 60S ribosome assembly; member of the dual-specificity family of protein phosphatases
0.109 2.63E-10 YDR452W PPN1 hom Vacuolar endopolyphosphatase with a role in phosphate metabolism; functions as a homodimer; relocalizes from vacuole to cytoplasm upon DNA replication stress
0.106 8.07E-10 YDL063C SYO1 hom Transport adaptor or symportin; facilitates synchronized nuclear coimport of the two 5S-rRNA binding proteins Rpl5p and Rpl11p; required for biogenesis of the large ribosomal subunit; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus
0.105 1.12E-9 YLR062C_d BUD28_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene RPL22A; diploid mutant displays a weak budding pattern phenotype in a systematic assay
0.102 3.76E-9 YNL067W-B_p YNL067W-B_p hom Putative protein of unknown function
0.098 1.16E-8 YNL227C JJJ1 hom Co-chaperone that stimulates the ATPase activity of Ssa1p, required for a late step of ribosome biogenesis; associated with the cytosolic large ribosomal subunit; contains a J-domain; mutation causes defects in fluid-phase endocytosis
0.097 2.00E-8 YPL101W ELP4 hom Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity
0.096 2.37E-8 YKR057W RPS21A hom Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S21, no bacterial homolog; RPS21A has a paralog, RPS21B, that arose from the whole genome duplication
0.093 6.56E-8 YOR312C RPL20B hom Ribosomal 60S subunit protein L20B; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20B has a paralog, RPL20A, that arose from the whole genome duplication
0.093 7.08E-8 YOR309C_d YOR309C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP58
0.084 9.62E-7 YGR271C-A EFG1 hom Essential protein required for maturation of 18S rRNA; null mutant is sensitive to hydroxyurea and is delayed in recovering from alpha-factor arrest; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus
0.084 9.86E-7 YGR219W_d YGR219W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRPL9/YGR220C
0.084 1.02E-6 YPL125W KAP120 hom Karyopherin responsible for the nuclear import of ribosome maturation factor Rfp1p