YGL086W / MAD1

Coiled-coil protein involved in the spindle-assembly checkpoint; required for inhibition of karyopherin/importin Pse1p (aka Kap121p) upon spindle assembly checkpoint arrest; phosphorylated by Mps1p upon checkpoint activation which leads to inhibition of the activity of the anaphase promoting complex; forms a complex with Mad2p

Zygosity: Homozygous strain
fixedexpanded
Profile for YGL086W / MAD1

Click on Significant Values for Screen Details ID:SGTC_6|Compound:0844-0013|FD-Score:2.98|P-value:9.79E-4 ID:SGTC_149|Compound:3970-0822|FD-Score:8.59|P-value:2.62E-19 ID:SGTC_156|Compound:3474-0145|FD-Score:3.09|P-value:6.82E-4 ID:SGTC_163|Compound:1327-0036|FD-Score:5.39|P-value:1.09E-8 ID:SGTC_286|Compound:k060-0067|FD-Score:4.60|P-value:9.06E-7 ID:SGTC_343|Compound:4130-2827|FD-Score:-3.12|P-value:6.07E-4 ID:SGTC_344|Compound:k064-0035|FD-Score:3.41|P-value:1.99E-4 ID:SGTC_367|Compound:0335-0881|FD-Score:4.58|P-value:1.03E-6 ID:SGTC_379|Compound:3970-0972|FD-Score:4.89|P-value:1.99E-7 ID:SGTC_397|Compound:arbidol|FD-Score:-3.18|P-value:4.99E-4 ID:SGTC_414|Compound:piperonyl butoxide|FD-Score:-3.14|P-value:5.77E-4 ID:SGTC_442|Compound:cape|FD-Score:4.56|P-value:1.11E-6 ID:SGTC_446|Compound:go6976|FD-Score:3.14|P-value:5.55E-4 ID:SGTC_451|Compound:chloral hydrate|FD-Score:3.23|P-value:4.05E-4 ID:SGTC_454|Compound:lyso-paf C-16|FD-Score:-3.34|P-value:2.69E-4 ID:SGTC_455|Compound:paf C-16|FD-Score:-4.30|P-value:4.23E-6 ID:SGTC_470|Compound:aa-861|FD-Score:3.16|P-value:5.26E-4 ID:SGTC_494|Compound:Boc-Gly-Val-Val-CHO|FD-Score:-3.55|P-value:1.19E-4 ID:SGTC_819|Compound:1013-0258|FD-Score:-2.98|P-value:9.95E-4 ID:SGTC_831|Compound:k061-1017|FD-Score:3.00|P-value:9.07E-4 ID:SGTC_888|Compound:0916-0002|FD-Score:-3.04|P-value:8.26E-4 ID:SGTC_1030|Compound:k029-0042|FD-Score:8.82|P-value:2.79E-20 ID:SGTC_1107|Compound:amlodipine|FD-Score:-4.41|P-value:2.46E-6 ID:SGTC_1108|Compound:betulinic acid|FD-Score:-6.38|P-value:1.84E-11 ID:SGTC_1111|Compound:tnp00062|FD-Score:-3.52|P-value:1.33E-4 ID:SGTC_1229|Compound:0139-0193|FD-Score:3.33|P-value:2.69E-4 ID:SGTC_1231|Compound:0242-0792|FD-Score:-3.33|P-value:2.75E-4 ID:SGTC_1415|Compound:3966-0071|FD-Score:-3.05|P-value:7.91E-4 ID:SGTC_1610|Compound:st001799|FD-Score:-3.17|P-value:5.09E-4 ID:SGTC_2034|Compound:5197015|FD-Score:3.35|P-value:2.49E-4 ID:SGTC_2099|Compound:5377090|FD-Score:3.03|P-value:8.30E-4 ID:SGTC_2103|Compound:5378138|FD-Score:3.18|P-value:4.73E-4 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:3.59|P-value:9.68E-5 ID:SGTC_2595|Compound:hexamethylquercetagetin|FD-Score:3.83|P-value:3.47E-5 ID:SGTC_2693|Compound:st077581|FD-Score:3.16|P-value:5.14E-4 ID:SGTC_2696|Compound:st077691|FD-Score:3.27|P-value:3.50E-4 ID:SGTC_2791|Compound:5663188|FD-Score:3.19|P-value:4.66E-4 ID:SGTC_2895|Compound:9054447|FD-Score:4.18|P-value:7.28E-6 ID:SGTC_2952|Compound:9073731|FD-Score:-3.70|P-value:6.44E-5 ID:SGTC_2962|Compound:9082311|FD-Score:8.75|P-value:5.42E-20 ID:SGTC_2989|Compound:9073649|FD-Score:3.67|P-value:7.10E-5 ID:SGTC_3049|Compound:9092223|FD-Score:3.85|P-value:3.23E-5 ID:SGTC_3345|Compound:9148410|FD-Score:3.90|P-value:2.58E-5 ID:SGTC_3353|Compound:9151644|FD-Score:-3.03|P-value:8.52E-4 ID:SGTC_6|Compound:0844-0013|FD-Score:2.98|P-value:9.79E-4 ID:SGTC_149|Compound:3970-0822|FD-Score:8.59|P-value:2.62E-19 ID:SGTC_156|Compound:3474-0145|FD-Score:3.09|P-value:6.82E-4 ID:SGTC_163|Compound:1327-0036|FD-Score:5.39|P-value:1.09E-8 ID:SGTC_286|Compound:k060-0067|FD-Score:4.60|P-value:9.06E-7 ID:SGTC_343|Compound:4130-2827|FD-Score:-3.12|P-value:6.07E-4 ID:SGTC_344|Compound:k064-0035|FD-Score:3.41|P-value:1.99E-4 ID:SGTC_367|Compound:0335-0881|FD-Score:4.58|P-value:1.03E-6 ID:SGTC_379|Compound:3970-0972|FD-Score:4.89|P-value:1.99E-7 ID:SGTC_397|Compound:arbidol|FD-Score:-3.18|P-value:4.99E-4 ID:SGTC_414|Compound:piperonyl butoxide|FD-Score:-3.14|P-value:5.77E-4 ID:SGTC_442|Compound:cape|FD-Score:4.56|P-value:1.11E-6 ID:SGTC_446|Compound:go6976|FD-Score:3.14|P-value:5.55E-4 ID:SGTC_451|Compound:chloral hydrate|FD-Score:3.23|P-value:4.05E-4 ID:SGTC_454|Compound:lyso-paf C-16|FD-Score:-3.34|P-value:2.69E-4 ID:SGTC_455|Compound:paf C-16|FD-Score:-4.30|P-value:4.23E-6 ID:SGTC_470|Compound:aa-861|FD-Score:3.16|P-value:5.26E-4 ID:SGTC_494|Compound:Boc-Gly-Val-Val-CHO|FD-Score:-3.55|P-value:1.19E-4 ID:SGTC_819|Compound:1013-0258|FD-Score:-2.98|P-value:9.95E-4 ID:SGTC_831|Compound:k061-1017|FD-Score:3.00|P-value:9.07E-4 ID:SGTC_888|Compound:0916-0002|FD-Score:-3.04|P-value:8.26E-4 ID:SGTC_1030|Compound:k029-0042|FD-Score:8.82|P-value:2.79E-20 ID:SGTC_1107|Compound:amlodipine|FD-Score:-4.41|P-value:2.46E-6 ID:SGTC_1108|Compound:betulinic acid|FD-Score:-6.38|P-value:1.84E-11 ID:SGTC_1111|Compound:tnp00062|FD-Score:-3.52|P-value:1.33E-4 ID:SGTC_1229|Compound:0139-0193|FD-Score:3.33|P-value:2.69E-4 ID:SGTC_1231|Compound:0242-0792|FD-Score:-3.33|P-value:2.75E-4 ID:SGTC_1415|Compound:3966-0071|FD-Score:-3.05|P-value:7.91E-4 ID:SGTC_1610|Compound:st001799|FD-Score:-3.17|P-value:5.09E-4 ID:SGTC_2034|Compound:5197015|FD-Score:3.35|P-value:2.49E-4 ID:SGTC_2099|Compound:5377090|FD-Score:3.03|P-value:8.30E-4 ID:SGTC_2103|Compound:5378138|FD-Score:3.18|P-value:4.73E-4 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:3.59|P-value:9.68E-5 ID:SGTC_2595|Compound:hexamethylquercetagetin|FD-Score:3.83|P-value:3.47E-5 ID:SGTC_2693|Compound:st077581|FD-Score:3.16|P-value:5.14E-4 ID:SGTC_2696|Compound:st077691|FD-Score:3.27|P-value:3.50E-4 ID:SGTC_2791|Compound:5663188|FD-Score:3.19|P-value:4.66E-4 ID:SGTC_2895|Compound:9054447|FD-Score:4.18|P-value:7.28E-6 ID:SGTC_2952|Compound:9073731|FD-Score:-3.70|P-value:6.44E-5 ID:SGTC_2962|Compound:9082311|FD-Score:8.75|P-value:5.42E-20 ID:SGTC_2989|Compound:9073649|FD-Score:3.67|P-value:7.10E-5 ID:SGTC_3049|Compound:9092223|FD-Score:3.85|P-value:3.23E-5 ID:SGTC_3345|Compound:9148410|FD-Score:3.90|P-value:2.58E-5 ID:SGTC_3353|Compound:9151644|FD-Score:-3.03|P-value:8.52E-4

Top fitness defect scores for YGL086W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1030 k029-0042 8.82 36.7 µM 2.79E-20 TSC3-RPN4 2 5.58E-19
2 SGTC_2962 9082311 8.75 13.2 µM 5.42E-20 1 1.04E-18
3 SGTC_149 3970-0822 8.59 46.1 µM 2.62E-19 tubulin folding & SWR complex 14 4.50E-18
4 SGTC_163 1327-0036 5.39 7.5 µM 1.09E-8 58 3.44E-8
5 SGTC_379 3970-0972 4.89 5.9 µM 1.99E-7 tubulin folding & SWR complex 14 5.14E-7
6 SGTC_286 k060-0067 4.60 22.9 µM 9.06E-7 tubulin folding & SWR complex 15 2.11E-6
7 SGTC_367 0335-0881 4.58 7.1 µM 1.03E-6 ubiquinone biosynthesis & proteasome 53 2.37E-6
8 SGTC_442 cape 4.56 100.0 µM 1.11E-6 5 2.54E-6
9 SGTC_2895 9054447 4.18 58.4 µM 7.28E-6 7 1.47E-5
10 SGTC_3345 9148410 3.90 3.6 µM 2.58E-5 9 4.80E-5
11 SGTC_3049 9092223 3.85 49.5 µM 3.23E-5 26 5.91E-5
12 SGTC_2595 hexamethylquercetagetin 3.83 100.0 µM 3.47E-5 29 6.32E-5
13 SGTC_2989 9073649 3.67 71.4 µM 7.10E-5 49 1.23E-4
14 SGTC_2494 18-aminoabieta-8,11,13-triene sulfate 3.59 3.7 µM 9.68E-5 116 1.65E-4
15 SGTC_344 k064-0035 3.41 6.4 µM 1.99E-4 60S ribosome export 40 3.22E-4
16 SGTC_2034 5197015 3.35 53.1 µM 2.49E-4 SWF1 & branched chain AA biosynthesis 39 3.97E-4
17 SGTC_1229 0139-0193 3.33 12.2 µM 2.69E-4 RSC complex & mRNA processing 29 4.28E-4
18 SGTC_2696 st077691 3.27 74.3 µM 3.50E-4 59 5.46E-4
19 SGTC_451 chloral hydrate 3.23 100.0 µM 4.05E-4 93 6.26E-4
20 SGTC_2791 5663188 3.19 71.4 µM 4.66E-4 RPP1 & pyrimidine depletion 52 7.14E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.231 7.34E-42 YBL034C STU1 het Component of the mitotic spindle that binds to interpolar microtubules via its association with beta-tubulin (Tub2p); required for interpolar microtubules to provide an outward force on the spindle poles
0.210 1.25E-34 YBR220C_p YBR220C_p hom Putative protein of unknown function; YBR220C is not an essential gene
0.181 3.18E-26 YNL305C BXI1 hom Protein involved in apoptosis; variously described as containing a BCL-2 homology (BH3) domain or as a member of the BAX inhibitor family; reported to promote apoptosis under some conditions and to inhibit it in others; localizes to ER and vacuole; may link the unfolded protein response to apoptosis via regulation of calcium-mediated signaling; translocates to mitochondria under apoptosis-inducing conditions in a process involving Mir1p and Cor1p
0.148 5.81E-18 YGR193C PDX1 hom Dihydrolipoamide dehydrogenase (E3)-binding protein (E3BP) of the mitochondrial pyruvate dehydrogenase (PDH) complex, plays a structural role in the complex by binding and positioning E3 to the dihydrolipoamide acetyltransferase (E2) core
0.147 1.39E-17 YFL063W_d YFL063W_d hom Dubious open reading frame, based on available experimental and comparative sequence data
0.135 5.06E-15 YER007W PAC2 hom Microtubule effector required for tubulin heterodimer formation, binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomyl
0.132 1.65E-14 YML035C AMD1 hom AMP deaminase; tetrameric enzyme that catalyzes the deamination of AMP to form IMP and ammonia; thought to be involved in regulation of intracellular purine (adenine, guanine, and inosine) nucleotide pools
0.131 2.42E-14 YLL051C FRE6 hom Putative ferric reductase with similarity to Fre2p; expression induced by low iron levels
0.130 4.72E-14 YDL018C ERP3 hom Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport
0.128 1.23E-13 YMR007W_d YMR007W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.122 1.28E-12 YJR013W GPI14 het Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M
0.121 1.61E-12 YML081C-A ATP18 hom Subunit of the mitochondrial F1F0 ATP synthase, which is a large enzyme complex required for ATP synthesis; termed subunit I or subunit j; does not correspond to known ATP synthase subunits in other organisms
0.119 4.23E-12 YER030W CHZ1 hom Histone chaperone for Htz1p/H2A-H2B dimer; required for the stabilization of the Chz1p-Htz1-H2B complex; has overlapping function with Nap1p; null mutant displays weak sensitivity to MMS and benomyl; contains a highly conserved CHZ motif; protein abundance increases in response to DNA replication stress
0.119 5.42E-12 YIR008C PRI1 het Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair
0.117 1.08E-11 YKL171W NNK1 hom Protein kinase; implicated in proteasome function; interacts with TORC1, Ure2 and Gdh2; overexpression leads to hypersensitivity to rapamycin and nuclear accumulation of Gln3; epitope-tagged protein localizes to the cytoplasm